Restriction map for
pFN21AE3136
(Circular) (Six-base) MAPSORT of: pFN21AE3136.seq Check: 8613 from: 1 to: 5749
pFN21AE3136 5749 bp
With 182 enzymes: SgfI *
July 6, 2012 17:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2404 2404
Size: 5749
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3559 278
Size: 53 83 186 936 2023 2468
Fragments arranged by size:
2468 2023 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3092 1937
Size: 1155 4594
Acc65I G'GTAC_C
Cuts at: 3070 3070
Size: 5749
AclI AA'CG_TT
Cuts at: 1563 3876 4249 1563
Size: 2313 373 3063
Fragments arranged by size:
3063 2313 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2801 3812 5196 5464 1632
Size: 1169 1011 1384 268 1917
Fragments arranged by size:
1917 1384 1169 1011 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5552
AflIII A'CryG_T
Cuts at: 1203 4648 5625 1203
Size: 3445 977 1327
Fragments arranged by size:
3445 1327 977
AgeI A'CCGG_T
Cuts at: 4580 4580
Size: 5749
AhdI GACnn_n'nnGTC
Cuts at: 4478 4478
Size: 5749
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5749
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5749
AlwNI CAG_nnn'CTG
Cuts at: 2191 3492 5064 2191
Size: 1301 1572 2876
Fragments arranged by size:
2876 1572 1301
ApaLI G'TGCA_C
Cuts at: 3806 4962 3806
Size: 1156 4593
ApoI r'AATT_y
Cuts at: 1493 3058 3284 3436 5545 1493
Size: 1565 226 152 2109 1697
Fragments arranged by size:
2109 1697 1565 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4303 160
Size: 872 3271 1606
Fragments arranged by size:
3271 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5749
AvaI C'yCGr_G
Cuts at: 1870 1943 2205 2288 3074 1870
Size: 73 262 83 786 4545
Fragments arranged by size:
4545 786 262 83 73
BamHI G'GATC_C
Cuts at: 3079 3446 3079
Size: 367 5382
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2047 2108 2171 3070 4525
Size: 325 217 627 260 61 63 899 1455
Cuts at: 4525 618
Size: 1842
Fragments arranged by size:
1842 1455 899 627 325 260 217 63
61
BanII G_rGCy'C
Cuts at: 1391 3068 1391
Size: 1677 4072
BbeI G_GCGC'C
Cuts at: 2051 2051
Size: 5749
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2334 2376 928
Size: 971 435 42 4301
Fragments arranged by size:
4301 971 435 42
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3940 3974 1903
Size: 34 2003 34 3678
Fragments arranged by size:
3678 2003 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2273 2308 2716 3641 4851 2273
Size: 35 408 925 1210 3171
Fragments arranged by size:
3171 1210 925 408 35
BclI T'GATC_A
Cuts at: 1548 2358 1548
Size: 810 4939
BfrBI ATG'CAT
Cuts at: 2964 2964
Size: 5749
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4360 136
Size: 107 122 71 631 605 2688 1525
Fragments arranged by size:
2688 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5744 5744
Size: 5749
BlpI GC'TnA_GC
Cuts at: 3166 3166
Size: 5749
Bme1580I G_kGCm'C
Cuts at: 1790 2542 3810 4966 1790
Size: 752 1268 1156 2573
Fragments arranged by size:
2573 1268 1156 752
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5749
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2618 3480 4438
Size: 416 53 83 399 24 1201 862 958
Cuts at: 4438 442
Size: 1753
Fragments arranged by size:
1753 1201 958 862 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5749
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2204 4409 1239
Size: 965 2205 2579
Fragments arranged by size:
2579 2205 965
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3874 4739 5037 3874
Size: 865 298 4586
Fragments arranged by size:
4586 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4412 882
Size: 617 2913 2219
Fragments arranged by size:
2913 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3540 493
Size: 711 2336 2702
Fragments arranged by size:
2702 2336 711
BsaBI GATnn'nnATC
Cuts at: 5743 5743
Size: 5749
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2048 2067 2554 3556
Size: 53 83 186 936 515 19 487 1002
Cuts at: 3556 3938 4573 275
Size: 382 635 1451
Fragments arranged by size:
1451 1002 936 635 515 487 382 186
83 53 19
BsaWI w'CCGG_w
Cuts at: 4182 4580 4854 5001 5412 4182
Size: 398 274 147 411 4519
Fragments arranged by size:
4519 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3033 3063 1178
Size: 30 165 30 1630 30 3864
Fragments arranged by size:
3864 1630 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2532 2679 2838 2854 1186
Size: 1346 147 159 16 4081
Fragments arranged by size:
4081 1346 159 147 16
BseYI C'CCAG_C
Cuts at: 2467 4952 5448 2467
Size: 2485 496 2768
Fragments arranged by size:
2768 2485 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5176
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3199 3960 4988 1540
Size: 275 186 1198 761 1028 2301
Fragments arranged by size:
2301 1198 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3810 3895 4966 3810
Size: 85 1071 4593
Fragments arranged by size:
4593 1071 85
BsmI GAATG_Cn'
Cuts at: 3260 3353 3260
Size: 93 5656
BsmBI CGTCTCn'nnnn_
Cuts at: 2074 2545 2074
Size: 471 5278
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2542 3068 3810 3895 4966 1391
Size: 399 752 526 742 85 1071 2174
Fragments arranged by size:
2174 1071 752 742 526 399 85
BspEI T'CCGG_A
Cuts at: 5412 5412
Size: 5749
BspHI T'CATG_A
Cuts at: 3638 5368 3638
Size: 1730 4019
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2404 3104 844
Size: 1560 700 3489
Fragments arranged by size:
3489 1560 700
BsrBI CCG'CTC
Cuts at: 1394 1823 3636 1394
Size: 429 1813 3507
Fragments arranged by size:
3507 1813 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2314 4244 4418 65
Size: 1183 1066 1930 174 1396
Fragments arranged by size:
1930 1396 1183 1066 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4393 4580 1700
Size: 110 2583 187 2869
Fragments arranged by size:
2869 2583 187 110
BsrGI T'GTAC_A
Cuts at: 96 2887 96
Size: 2791 2958
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5749
BssSI C'ACGA_G
Cuts at: 3809 4821 3809
Size: 1012 4737
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5749
BstBI TT'CG_AA
Cuts at: 4553 4553
Size: 5749
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5749
BstXI CCAn_nnnn'nTGG
Cuts at: 2461 2461
Size: 5749
BstYI r'GATC_y
Cuts at: 1972 2299 3079 3446 3831 3848 5300 5386 5398
Size: 327 780 367 385 17 1452 86 12
Cuts at: 5398 5744 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 780 385 367 346 327 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2103 2460 513
Size: 552 1038 357 3802
Fragments arranged by size:
3802 1038 552 357
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5010
Fragments arranged by size:
5010 683 56
BtsI GCAGTG_nn'
Cuts at: 3265 4059 4079 3265
Size: 794 20 4935
Fragments arranged by size:
4935 794 20
ClaI AT'CG_AT
Cuts at: 1554 2681 1554
Size: 1127 4622
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5714
DraI TTT'AAA
Cuts at: 3053 3053
Size: 5749
DraIII CAC_nnn'GTG
Cuts at: 2142 2142
Size: 5749
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4756 809
Size: 507 3440 1802
Fragments arranged by size:
3440 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3196 4085 5714
Size: 54 1425 50 275 18 1366 889 1629
Cuts at: 5714 8
Size: 43
Fragments arranged by size:
1629 1425 1366 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3196 1537
Size: 275 1384 4090
Fragments arranged by size:
4090 1384 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3679 1353
Size: 2326 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4336 4710 4856 387
Size: 1064 138 2747 374 146 1280
Fragments arranged by size:
2747 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2204 2801 3812 4409 5196 5464 1239
Size: 393 572 597 1011 597 787 268 1524
Fragments arranged by size:
1524 1011 787 597 597 572 393 268
EcoRI G'AATT_C
Cuts at: 3058 3436 3058
Size: 378 5371
EcoRV GAT'ATC
Cuts at: 2679 2679
Size: 5749
FspI TGC'GCA
Cuts at: 4255 4255
Size: 5749
HaeII r_GCGC'y
Cuts at: 1678 2051 4896 1678
Size: 373 2845 2531
Fragments arranged by size:
2845 2531 373
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4390 4422 4464 4496 1373
Size: 32 135 32 2818 32 42 32 2626
Fragments arranged by size:
2818 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2944 3093 3339 669
Size: 988 281 1006 149 246 3079
Fragments arranged by size:
3079 1006 988 281 246 149
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5749
HpaI GTT'AAC
Cuts at: 3339 3339
Size: 5749
KasI G'GCGC_C
Cuts at: 2047 2047
Size: 5749
KpnI G_GTAC'C
Cuts at: 3074 3074
Size: 5749
MfeI C'AATT_G
Cuts at: 3348 3348
Size: 5749
MluI A'CGCG_T
Cuts at: 5625 5625
Size: 5749
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2257 4863 5047 1305
Size: 952 2606 184 2007
Fragments arranged by size:
2606 2007 952 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3927
Fragments arranged by size:
3927 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3707 4066 4225 518
Size: 648 2541 359 159 2042
Fragments arranged by size:
2541 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2105 2154 3165 3842 4990 5235 1606
Size: 499 49 1011 677 1148 245 2120
Fragments arranged by size:
2120 1148 1011 677 499 245 49
NarI GG'CG_CC
Cuts at: 2048 2048
Size: 5749
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5197
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5749
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5749
NotI GC'GGCC_GC
Cuts at: 3196 3196
Size: 5749
NsiI A_TGCA'T
Cuts at: 2966 2966
Size: 5749
NspI r_CATG'y
Cuts at: 1132 1229 2356 3014 3107 4652 1132
Size: 97 1127 658 93 1545 2229
Fragments arranged by size:
2229 1545 1127 658 97 93
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5749
PciI A'CATG_T
Cuts at: 4648 4648
Size: 5749
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5749
PmeI GTTT'AAAC
Cuts at: 3053 3053
Size: 5749
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3818 3850 5331 5363 3818
Size: 32 1481 32 4204
Fragments arranged by size:
4204 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5749
PsiI TTA'TAA
Cuts at: 3319 3319
Size: 5749
PstI C_TGCA'G
Cuts at: 830 3101 830
Size: 2271 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5749
RsrII CG'GwC_CG
Cuts at: 2130 2130
Size: 5749
SacII CC_GC'GG
Cuts at: 2106 2106
Size: 5749
SalI G'TCGA_C
Cuts at: 1936 3091 1936
Size: 1155 4594
SbfI CC_TGCA'GG
Cuts at: 3101 3101
Size: 5749
ScaI AGT'ACT
Cuts at: 2630 3997 5709 2630
Size: 1367 1712 2670
Fragments arranged by size:
2670 1712 1367
SfcI C'TryA_G
Cuts at: 826 1045 2593 3097 4232 4913 5104 826
Size: 219 1548 504 1135 681 191 1471
Fragments arranged by size:
1548 1471 1135 681 504 219 191
SfoI GGC'GCC
Cuts at: 2049 2049
Size: 5749
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5749
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5749
SmaI CCC'GGG
Cuts at: 1872 2207 3076 1872
Size: 335 869 4545
Fragments arranged by size:
4545 869 335
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3853 4754 5016 820
Size: 197 926 1910 901 262 1553
Fragments arranged by size:
1910 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3540 493
Size: 3047 2702
SpeI A'CTAG_T
Cuts at: 152 2492 152
Size: 2340 3409
SphI G_CATG'C
Cuts at: 1132 3107 1132
Size: 1975 3774
SspI AAT'ATT
Cuts at: 5 52 3673 5
Size: 47 3621 2081
Fragments arranged by size:
3621 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5749
StyI C'CwwG_G
Cuts at: 513 1065 2163 3015 3188 513
Size: 552 1098 852 173 3074
Fragments arranged by size:
3074 1098 852 552 173
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2147 2189 3776 3793 3946 4131 4576
Size: 1255 567 42 1587 17 153 185 445
Cuts at: 4576 325
Size: 1498
Fragments arranged by size:
1587 1498 1255 567 445 185 153 42
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2499 2628 2887
Size: 274 80 33 51 1443 522 129 259
Cuts at: 2887 3995 5707 96
Size: 1108 1712 138
Fragments arranged by size:
1712 1443 1108 522 274 259 138 129
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5709 5741 5709
Size: 32 5717
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5749
XbaI T'CTAG_A
Cuts at: 3085 3085
Size: 5749
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2048 3035 2048
Size: 987 4762
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5749
XmaI C'CCGG_G
Cuts at: 1870 2205 3074 1870
Size: 335 869 4545
Fragments arranged by size:
4545 869 335
XmnI GAAnn'nnTTC
Cuts at: 3878 3878
Size: 5749
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3557 276
Size: 53 83 186 936 2023 2468
Fragments arranged by size:
2468 2023 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI RsrII SacII SalI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbvCI BplI
Bpu10I BsiWI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I FalI
FseI FspAI NaeI NgoMIV NruI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI PvuII SacI SanDI SapI
SexAI SfiI SrfI SwaI