Restriction map for
pFN21AE3135
(Circular) (Six-base) MAPSORT of: pFN21AE3135.seq Check: 2267 from: 1 to: 5740
pFN21AE3135 5740 bp
With 182 enzymes: SgfI *
December 28, 2011 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3550 278
Size: 53 83 186 936 2014 2468
Fragments arranged by size:
2468 2014 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3083 1937
Size: 1146 4594
Acc65I G'GTAC_C
Cuts at: 3061 3061
Size: 5740
AclI AA'CG_TT
Cuts at: 1563 3867 4240 1563
Size: 2304 373 3063
Fragments arranged by size:
3063 2304 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2237 3803 5187 5455 1632
Size: 605 1566 1384 268 1917
Fragments arranged by size:
1917 1566 1384 605 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5543
AflIII A'CryG_T
Cuts at: 1203 4639 5616 1203
Size: 3436 977 1327
Fragments arranged by size:
3436 1327 977
AgeI A'CCGG_T
Cuts at: 4571 4571
Size: 5740
AhdI GACnn_n'nnGTC
Cuts at: 4469 4469
Size: 5740
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5740
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5740
AlwNI CAG_nnn'CTG
Cuts at: 2897 3483 5055 2897
Size: 586 1572 3582
Fragments arranged by size:
3582 1572 586
ApaLI G'TGCA_C
Cuts at: 3797 4953 3797
Size: 1156 4584
ApoI r'AATT_y
Cuts at: 1493 2204 2269 2383 2432 2733 3049 3275 3427
Size: 711 65 114 49 301 316 226 152
Cuts at: 3427 5536 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 711 316 301 226 152 114
65 49
AseI AT'TA_AT
Cuts at: 160 1032 4294 160
Size: 872 3262 1606
Fragments arranged by size:
3262 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5740
AvaI C'yCGr_G
Cuts at: 1870 1943 2192 3065 1870
Size: 73 249 873 4545
Fragments arranged by size:
4545 873 249 73
BamHI G'GATC_C
Cuts at: 3070 3437 3070
Size: 367 5373
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3061 4516 618
Size: 325 217 627 1274 1455 1842
Fragments arranged by size:
1842 1455 1274 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2025 3059 1391
Size: 634 1034 4072
Fragments arranged by size:
4072 1034 634
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4769
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3931 3965 1903
Size: 34 50 34 1910 34 3678
Fragments arranged by size:
3678 1910 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3632 4842 3632
Size: 1210 4530
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5740
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2020 4351 136
Size: 107 122 71 631 605 348 2331 1525
Fragments arranged by size:
2331 1525 631 605 348 122 107 71
BglII A'GATC_T
Cuts at: 2910 5735 2910
Size: 2825 2915
BlpI GC'TnA_GC
Cuts at: 3157 3157
Size: 5740
Bme1580I G_kGCm'C
Cuts at: 1790 3801 4957 1790
Size: 2011 1156 2573
Fragments arranged by size:
2573 2011 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5740
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2185 3471 4429
Size: 416 53 83 399 24 768 1286 958
Cuts at: 4429 442
Size: 1753
Fragments arranged by size:
1753 1286 958 768 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5740
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4400 1239
Size: 3161 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2537 3865 4730 5028 2537
Size: 1328 865 298 3249
Fragments arranged by size:
3249 1328 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4403 882
Size: 617 2904 2219
Fragments arranged by size:
2904 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3531 493
Size: 711 2327 2702
Fragments arranged by size:
2702 2327 711
BsaBI GATnn'nnATC
Cuts at: 5734 5734
Size: 5740
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3547 3929 4564 275
Size: 53 83 186 936 2014 382 635 1451
Fragments arranged by size:
2014 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4173 4571 4845 4992 5403 4173
Size: 398 274 147 411 4510
Fragments arranged by size:
4510 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5515
Fragments arranged by size:
5515 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2237 1186
Size: 1051 4689
BseYI C'CCAG_C
Cuts at: 2062 4943 5439 2062
Size: 2881 496 2363
Fragments arranged by size:
2881 2363 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5167
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2015 2149 3190 3951 4979 1540
Size: 275 186 14 134 1041 761 1028 2301
Fragments arranged by size:
2301 1041 1028 761 275 186 134 14
BsiHKAI G_wGCw'C
Cuts at: 2515 3801 3886 4957 2515
Size: 1286 85 1071 3298
Fragments arranged by size:
3298 1286 1071 85
BsmI GAATG_Cn'
Cuts at: 3251 3344 3251
Size: 93 5647
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2025 2515 3059 3801 3886 4957 1391
Size: 399 235 490 544 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 544 490 399 235 85
BspEI T'CCGG_A
Cuts at: 5403 5403
Size: 5740
BspHI T'CATG_A
Cuts at: 2938 3629 5359 2938
Size: 691 1730 3319
Fragments arranged by size:
3319 1730 691
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2905 3095 844
Size: 2061 190 3489
Fragments arranged by size:
3489 2061 190
BsrBI CCG'CTC
Cuts at: 1394 1823 3627 1394
Size: 429 1804 3507
Fragments arranged by size:
3507 1804 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2310 2361 2571 4235 4409 65
Size: 1183 1062 51 210 1664 174 1396
Fragments arranged by size:
1664 1396 1183 1062 210 174 51
BsrFI r'CCGG_y
Cuts at: 1700 1810 2014 4384 4571 1700
Size: 110 204 2370 187 2869
Fragments arranged by size:
2869 2370 204 187 110
BsrGI T'GTAC_A
Cuts at: 96 2859 2871 96
Size: 2763 12 2965
Fragments arranged by size:
2965 2763 12
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5740
BssSI C'ACGA_G
Cuts at: 3800 4812 3800
Size: 1012 4728
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2372 794
Size: 1578 4162
BstBI TT'CG_AA
Cuts at: 4544 4544
Size: 5740
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5740
BstYI r'GATC_y
Cuts at: 1972 2287 2910 3070 3437 3822 3839 5291 5377
Size: 315 623 160 367 385 17 1452 86
Cuts at: 5377 5389 5735 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 623 385 367 346 315 160
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2110 513
Size: 552 1045 4143
Fragments arranged by size:
4143 1045 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5001
Fragments arranged by size:
5001 683 56
BtsI GCAGTG_nn'
Cuts at: 2661 2862 3256 4050 4070 2661
Size: 201 394 794 20 4331
Fragments arranged by size:
4331 794 394 201 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5740
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5705
DraI TTT'AAA
Cuts at: 2538 3044 2538
Size: 506 5234
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4747 809
Size: 507 3431 1802
Fragments arranged by size:
3431 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2012 2077 3187
Size: 54 1425 50 275 18 182 65 1110
Cuts at: 3187 4076 5705 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1110 889 275 182 65 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2012 3187 1537
Size: 275 200 1175 4090
Fragments arranged by size:
4090 1175 275 200
EarI CTCTTCn'nnn_
Cuts at: 1353 2296 3670 1353
Size: 943 1374 3423
Fragments arranged by size:
3423 1374 943
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4327 4701 4847 387
Size: 1064 138 2738 374 146 1280
Fragments arranged by size:
2738 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2237 3803 4400 5187 5455 1239
Size: 393 605 1566 597 787 268 1524
Fragments arranged by size:
1566 1524 787 605 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2219 2219
Size: 5740
EcoO109I rG'GnC_Cy
Cuts at: 2059 2059
Size: 5740
EcoRI G'AATT_C
Cuts at: 2432 3049 3427 2432
Size: 617 378 4745
Fragments arranged by size:
4745 617 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2453 2453
Size: 5740
FspI TGC'GCA
Cuts at: 4246 4246
Size: 5740
HaeII r_GCGC'y
Cuts at: 1678 2032 4887 1678
Size: 354 2855 2531
Fragments arranged by size:
2855 2531 354
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2541 2573 4381 4413 4455
Size: 32 135 32 969 32 1808 32 42
Cuts at: 4455 4487 1373
Size: 32 2626
Fragments arranged by size:
2626 1808 969 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2743 3084 3330 669
Size: 988 281 805 341 246 3079
Fragments arranged by size:
3079 988 805 341 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5740
HpaI GTT'AAC
Cuts at: 2743 3330 2743
Size: 587 5153
KpnI G_GTAC'C
Cuts at: 3065 3065
Size: 5740
MfeI C'AATT_G
Cuts at: 3339 3339
Size: 5740
MluI A'CGCG_T
Cuts at: 5616 5616
Size: 5740
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2209 2216 4854 5038 1305
Size: 904 7 2638 184 2007
Fragments arranged by size:
2638 2007 904 184 7
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3918
Fragments arranged by size:
3918 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3698 4057 4216 518
Size: 648 2532 359 159 2042
Fragments arranged by size:
2532 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2066 2112 2849 2960 3156 3833 4981 5226
Size: 460 46 737 111 196 677 1148 245
Cuts at: 5226 1606
Size: 2120
Fragments arranged by size:
2120 1148 737 677 460 245 196 111
46
NaeI GCC'GGC
Cuts at: 2016 2016
Size: 5740
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5188
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5740
NgoMIV G'CCGG_C
Cuts at: 2014 2014
Size: 5740
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5740
NotI GC'GGCC_GC
Cuts at: 3187 3187
Size: 5740
NspI r_CATG'y
Cuts at: 1132 1229 3098 4643 1132
Size: 97 1869 1545 2229
Fragments arranged by size:
2229 1869 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5740
PciI A'CATG_T
Cuts at: 4639 4639
Size: 5740
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5740
PfoI T'CCnGG_A
Cuts at: 2749 2749
Size: 5740
PmeI GTTT'AAAC
Cuts at: 3044 3044
Size: 5740
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3809 3841 5322 5354 3809
Size: 32 1481 32 4195
Fragments arranged by size:
4195 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2059 2059
Size: 5740
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5740
PsiI TTA'TAA
Cuts at: 3310 3310
Size: 5740
PstI C_TGCA'G
Cuts at: 830 3092 830
Size: 2262 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5740
PvuII CAG'CTG
Cuts at: 2849 2960 2849
Size: 111 5629
SacII CC_GC'GG
Cuts at: 2113 2113
Size: 5740
SalI G'TCGA_C
Cuts at: 1936 3082 1936
Size: 1146 4594
SanDI GG'GwC_CC
Cuts at: 2059 2059
Size: 5740
SbfI CC_TGCA'GG
Cuts at: 3092 3092
Size: 5740
ScaI AGT'ACT
Cuts at: 3988 5700 3988
Size: 1712 4028
SexAI A'CCwGG_T
Cuts at: 2463 2463
Size: 5740
SfcI C'TryA_G
Cuts at: 826 1045 3088 4223 4904 5095 826
Size: 219 2043 1135 681 191 1471
Fragments arranged by size:
2043 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5740
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5740
SmaI CCC'GGG
Cuts at: 1872 3067 1872
Size: 1195 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2516 3844 4745 5007 820
Size: 197 926 573 1328 901 262 1553
Fragments arranged by size:
1553 1328 926 901 573 262 197
SnaBI TAC'GTA
Cuts at: 493 3531 493
Size: 3038 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5740
SphI G_CATG'C
Cuts at: 1132 3098 1132
Size: 1966 3774
SspI AAT'ATT
Cuts at: 5 52 2140 2321 3664 5
Size: 47 2088 181 1343 2081
Fragments arranged by size:
2088 2081 1343 181 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5740
StyI C'CwwG_G
Cuts at: 513 1065 2045 3179 513
Size: 552 980 1134 3074
Fragments arranged by size:
3074 1134 980 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2482 2777 3767 3784 3937 4122 4567
Size: 1255 902 295 990 17 153 185 445
Cuts at: 4567 325
Size: 1498
Fragments arranged by size:
1498 1255 990 902 445 295 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2859 2871 3986
Size: 274 80 33 51 1443 882 12 1115
Cuts at: 3986 5698 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1115 882 274 138 80 51
33 12
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5700 5732 5700
Size: 32 5708
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5740
XbaI T'CTAG_A
Cuts at: 3076 3076
Size: 5740
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5740
XmaI C'CCGG_G
Cuts at: 1870 3065 1870
Size: 1195 4545
XmnI GAAnn'nnTTC
Cuts at: 2141 2436 3869 2141
Size: 295 1433 4012
Fragments arranged by size:
4012 1433 295
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3548 276
Size: 53 83 186 936 2014 2468
Fragments arranged by size:
2468 2014 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SacII SalI SanDI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI NarI
NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII
SacI SapI SfiI SfoI SrfI SwaI XcmI