Restriction map for
pFN21AE3131
(Circular) (Six-base) MAPSORT of: pFN21AE3131.seq Check: 1146 from: 1 to: 5692
pFN21AE3131 5692 bp
With 182 enzymes: SgfI *
March 28, 2012 13:15 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2622 3502 278
Size: 53 83 186 936 1086 880 2468
Fragments arranged by size:
2468 1086 936 880 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3035 1937
Size: 1098 4594
Acc65I G'GTAC_C
Cuts at: 3013 3013
Size: 5692
AclI AA'CG_TT
Cuts at: 1563 3819 4192 1563
Size: 2256 373 3063
Fragments arranged by size:
3063 2256 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3755 5139 5407 1632
Size: 2123 1384 268 1917
Fragments arranged by size:
2123 1917 1384 268
AfeI AGC'GCT
Cuts at: 2166 2546 2166
Size: 380 5312
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5495
AflIII A'CryG_T
Cuts at: 1203 2938 4591 5568 1203
Size: 1735 1653 977 1327
Fragments arranged by size:
1735 1653 1327 977
AgeI A'CCGG_T
Cuts at: 4523 4523
Size: 5692
AhdI GACnn_n'nnGTC
Cuts at: 4421 4421
Size: 5692
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5692
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5692
AlwNI CAG_nnn'CTG
Cuts at: 3435 5007 3435
Size: 1572 4120
ApaI G_GGCC'C
Cuts at: 2260 2914 2260
Size: 654 5038
ApaLI G'TGCA_C
Cuts at: 3749 4905 3749
Size: 1156 4536
ApoI r'AATT_y
Cuts at: 1493 3001 3227 3379 5488 1493
Size: 1508 226 152 2109 1697
Fragments arranged by size:
2109 1697 1508 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4246 160
Size: 872 3214 1606
Fragments arranged by size:
3214 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5692
AvaI C'yCGr_G
Cuts at: 1870 1943 2478 3017 1870
Size: 73 535 539 4545
Fragments arranged by size:
4545 539 535 73
BamHI G'GATC_C
Cuts at: 2337 3022 3389 2337
Size: 685 367 4640
Fragments arranged by size:
4640 685 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2554 3013 4468 618
Size: 325 217 627 767 459 1455 1842
Fragments arranged by size:
1842 1455 767 627 459 325 217
BanII G_rGCy'C
Cuts at: 1391 2104 2260 2479 2914 3011 1391
Size: 713 156 219 435 97 4072
Fragments arranged by size:
4072 713 435 219 156 97
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2077 2344 2719 928
Size: 971 178 267 375 3901
Fragments arranged by size:
3901 971 375 267 178
BbvCI CC'TCA_GC
Cuts at: 2889 2889
Size: 5692
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3883 3917 1903
Size: 34 50 34 1862 34 3678
Fragments arranged by size:
3678 1862 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3584 4794 3584
Size: 1210 4482
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5692
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4303 136
Size: 107 122 71 631 605 2631 1525
Fragments arranged by size:
2631 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2173 2751 5687 2173
Size: 578 2936 2178
Fragments arranged by size:
2936 2178 578
BlpI GC'TnA_GC
Cuts at: 3109 3109
Size: 5692
Bme1580I G_kGCm'C
Cuts at: 1790 2260 2559 2914 3753 4909 1790
Size: 470 299 355 839 1156 2573
Fragments arranged by size:
2573 1156 839 470 355 299
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5692
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2599 2916 3423
Size: 416 53 83 399 24 1182 317 507
Cuts at: 3423 4381 442
Size: 958 1753
Fragments arranged by size:
1753 1182 958 507 416 399 317 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5692
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2393 2393
Size: 5692
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2342 2414 2654 2660 2732 2833 4352 1239
Size: 1103 72 240 6 72 101 1519 2579
Fragments arranged by size:
2579 1519 1103 240 101 72 72 6
Bpu10I CC'TnA_GC
Cuts at: 2451 2889 2970 2451
Size: 438 81 5173
Fragments arranged by size:
5173 438 81
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2267 3817 4682 4980 2267
Size: 1550 865 298 2979
Fragments arranged by size:
2979 1550 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2312 2364 4355 882
Size: 617 813 52 1991 2219
Fragments arranged by size:
2219 1991 813 617 52
BsaAI yAC'GTr
Cuts at: 493 1204 3483 493
Size: 711 2279 2702
Fragments arranged by size:
2702 2279 711
BsaBI GATnn'nnATC
Cuts at: 5686 5686
Size: 5692
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2220 2619 3499 3881
Size: 53 83 186 936 687 399 880 382
Cuts at: 3881 4516 275
Size: 635 1451
Fragments arranged by size:
1451 936 880 687 635 399 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4125 4523 4797 4944 5355 4125
Size: 398 274 147 411 4462
Fragments arranged by size:
4462 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5467
Fragments arranged by size:
5467 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2735 1186
Size: 1549 4143
BseYI C'CCAG_C
Cuts at: 2095 2233 2534 2930 4895 5391 2095
Size: 138 301 396 1965 496 2396
Fragments arranged by size:
2396 1965 496 396 301 138
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2503 1163
Size: 573 767 4352
Fragments arranged by size:
4352 767 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3142 3903 4931 1540
Size: 275 186 1141 761 1028 2301
Fragments arranged by size:
2301 1141 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3753 3838 4909 3753
Size: 85 1071 4536
Fragments arranged by size:
4536 1071 85
BsmI GAATG_Cn'
Cuts at: 3203 3296 3203
Size: 93 5599
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2104 2260 2479 2559 2914 3011 3753
Size: 399 314 156 219 80 355 97 742
Cuts at: 3753 3838 4909 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 355 314 219 156
97 85 80
BspEI T'CCGG_A
Cuts at: 5355 5355
Size: 5692
BspHI T'CATG_A
Cuts at: 3581 5311 3581
Size: 1730 3962
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3047 844
Size: 2203 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3579 1394
Size: 429 1756 3507
Fragments arranged by size:
3507 1756 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4187 4361 65
Size: 1183 2939 174 1396
Fragments arranged by size:
2939 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4336 4523 1700
Size: 110 2526 187 2869
Fragments arranged by size:
2869 2526 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5692
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5692
BssSI C'ACGA_G
Cuts at: 2780 3752 4764 2780
Size: 972 1012 3708
Fragments arranged by size:
3708 1012 972
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5692
BstBI TT'CG_AA
Cuts at: 4496 4496
Size: 5692
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5692
BstXI CCAn_nnnn'nTGG
Cuts at: 2756 2756
Size: 5692
BstYI r'GATC_y
Cuts at: 1972 2173 2337 2751 3022 3389 3774 3791 5243
Size: 201 164 414 271 367 385 17 1452
Cuts at: 5243 5329 5341 5687 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 414 385 367 346 271 201
164 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2037 2037
Size: 5692
BtgI C'CryG_G
Cuts at: 513 1065 2877 513
Size: 552 1812 3328
Fragments arranged by size:
3328 1812 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2722 487
Size: 683 56 1496 3457
Fragments arranged by size:
3457 1496 683 56
BtsI GCAGTG_nn'
Cuts at: 2805 3208 4002 4022 2805
Size: 403 794 20 4475
Fragments arranged by size:
4475 794 403 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5692
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5657
DraI TTT'AAA
Cuts at: 2983 2996 2983
Size: 13 5679
DraIII CAC_nnn'GTG
Cuts at: 2607 2607
Size: 5692
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4699 809
Size: 507 3383 1802
Fragments arranged by size:
3383 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3139 4028 5657
Size: 54 1425 50 275 18 1309 889 1629
Cuts at: 5657 8
Size: 43
Fragments arranged by size:
1629 1425 1309 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3139 1537
Size: 275 1327 4090
Fragments arranged by size:
4090 1327 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2390 3622 1353
Size: 1037 1232 3423
Fragments arranged by size:
3423 1232 1037
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4279 4653 4799 387
Size: 1064 138 2690 374 146 1280
Fragments arranged by size:
2690 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2342 2414 2654 2660 2732 2833 3755
Size: 393 710 72 240 6 72 101 922
Cuts at: 3755 4352 5139 5407 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 922 787 710 597 393 268 240
101 72 72 6
EcoO109I rG'GnC_Cy
Cuts at: 2256 2257 2483 2578 2869 2885 2910 2911 2256
Size: 1 226 95 291 16 25 1 5037
Fragments arranged by size:
5037 291 226 95 25 16 1 1
EcoRI G'AATT_C
Cuts at: 3001 3379 3001
Size: 378 5314
FspI TGC'GCA
Cuts at: 4198 4198
Size: 5692
HaeII r_GCGC'y
Cuts at: 1678 2168 2548 4839 1678
Size: 490 380 2291 2531
Fragments arranged by size:
2531 2291 490 380
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2858 2890 4333 4365 4407
Size: 32 135 32 1286 32 1443 32 42
Cuts at: 4407 4439 1373
Size: 32 2626
Fragments arranged by size:
2626 1443 1286 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3036 3282 669
Size: 988 281 1098 246 3079
Fragments arranged by size:
3079 1098 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5692
HpaI GTT'AAC
Cuts at: 3282 3282
Size: 5692
KpnI G_GTAC'C
Cuts at: 3017 3017
Size: 5692
MfeI C'AATT_G
Cuts at: 3291 3291
Size: 5692
MluI A'CGCG_T
Cuts at: 5568 5568
Size: 5692
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2194 4806 4990 1305
Size: 889 2612 184 2007
Fragments arranged by size:
2612 2007 889 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3870
Fragments arranged by size:
3870 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2228 3650 4009 4168 518
Size: 648 1062 1422 359 159 2042
Fragments arranged by size:
2042 1422 1062 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3108 3785 4933 5178 1606
Size: 1502 677 1148 245 2120
Fragments arranged by size:
2120 1502 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5140
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5692
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5692
NotI GC'GGCC_GC
Cuts at: 3139 3139
Size: 5692
NspI r_CATG'y
Cuts at: 1132 1229 2942 3050 4595 1132
Size: 97 1713 108 1545 2229
Fragments arranged by size:
2229 1713 1545 108 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5692
PciI A'CATG_T
Cuts at: 2938 4591 2938
Size: 1653 4039
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2493 1108
Size: 1385 4307
PfoI T'CCnGG_A
Cuts at: 2262 2319 2262
Size: 57 5635
PmeI GTTT'AAAC
Cuts at: 2996 2996
Size: 5692
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3761 3793 5274 5306 3761
Size: 32 1481 32 4147
Fragments arranged by size:
4147 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2578 2869 2578
Size: 291 5401
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5692
PsiI TTA'TAA
Cuts at: 3262 3262
Size: 5692
PspOMI G'GGCC_C
Cuts at: 2256 2910 2256
Size: 654 5038
PstI C_TGCA'G
Cuts at: 830 2700 3044 830
Size: 1870 344 3478
Fragments arranged by size:
3478 1870 344
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5692
SalI G'TCGA_C
Cuts at: 1936 3034 1936
Size: 1098 4594
SanDI GG'GwC_CC
Cuts at: 2578 2578
Size: 5692
SbfI CC_TGCA'GG
Cuts at: 3044 3044
Size: 5692
ScaI AGT'ACT
Cuts at: 3940 5652 3940
Size: 1712 3980
SfcI C'TryA_G
Cuts at: 826 1045 2696 3040 4175 4856 5047 826
Size: 219 1651 344 1135 681 191 1471
Fragments arranged by size:
1651 1471 1135 681 344 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5692
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5692
SmaI CCC'GGG
Cuts at: 1872 3019 1872
Size: 1147 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2282 3796 4697 4959 820
Size: 197 926 339 1514 901 262 1553
Fragments arranged by size:
1553 1514 926 901 339 262 197
SnaBI TAC'GTA
Cuts at: 493 3483 493
Size: 2990 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5692
SphI G_CATG'C
Cuts at: 1132 3050 1132
Size: 1918 3774
SspI AAT'ATT
Cuts at: 5 52 3616 5
Size: 47 3564 2081
Fragments arranged by size:
3564 2081 47
StuI AGG'CCT
Cuts at: 1517 2184 2408 1517
Size: 667 224 4801
Fragments arranged by size:
4801 667 224
StyI C'CwwG_G
Cuts at: 513 1065 3131 513
Size: 552 2066 3074
Fragments arranged by size:
3074 2066 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2080 2294 2618 2944 2945 3719 3736
Size: 1255 500 214 324 326 1 774 17
Cuts at: 3736 3889 4074 4519 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1255 774 500 445 326 324 214
185 153 17 1
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3938 5650 96
Size: 274 80 33 51 1443 1961 1712 138
Fragments arranged by size:
1961 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5652 5684 5652
Size: 32 5660
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5692
XbaI T'CTAG_A
Cuts at: 3028 3028
Size: 5692
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2671 2671
Size: 5692
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5692
XmaI C'CCGG_G
Cuts at: 1870 3017 1870
Size: 1147 4545
XmnI GAAnn'nnTTC
Cuts at: 2060 2273 3821 2060
Size: 213 1548 3931
Fragments arranged by size:
3931 1548 213
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2620 3500 276
Size: 53 83 186 936 1086 880 2468
Fragments arranged by size:
2468 1086 936 880 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI
SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BfrBI BsiWI
BsmBI BstZ17I EcoICRI EcoNI EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PspXI PsrI PvuII RsrII SacI SacII SapI SexAI
SfiI SfoI SrfI SwaI