Restriction map for
pFN21AE3121
(Circular) (Six-base) MAPSORT of: pFN21AE3121.seq Check: 8868 from: 1 to: 5626
pFN21AE3121 5626 bp
With 182 enzymes: SgfI *
March 28, 2012 13:15 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2358 3436 278
Size: 53 83 186 936 822 1078 2468
Fragments arranged by size:
2468 1078 936 822 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2969 1937
Size: 1032 4594
Acc65I G'GTAC_C
Cuts at: 2947 2947
Size: 5626
AclI AA'CG_TT
Cuts at: 1563 3753 4126 1563
Size: 2190 373 3063
Fragments arranged by size:
3063 2190 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3689 5073 5341 1632
Size: 2057 1384 268 1917
Fragments arranged by size:
2057 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5429
AflIII A'CryG_T
Cuts at: 1203 2649 4525 5502 1203
Size: 1446 1876 977 1327
Fragments arranged by size:
1876 1446 1327 977
AgeI A'CCGG_T
Cuts at: 4457 4457
Size: 5626
AhdI GACnn_n'nnGTC
Cuts at: 2069 4355 2069
Size: 2286 3340
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5626
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5626
AlwNI CAG_nnn'CTG
Cuts at: 2822 3369 4941 2822
Size: 547 1572 3507
Fragments arranged by size:
3507 1572 547
ApaLI G'TGCA_C
Cuts at: 3683 4839 3683
Size: 1156 4470
ApoI r'AATT_y
Cuts at: 1493 2333 2935 3161 3313 5422 1493
Size: 840 602 226 152 2109 1697
Fragments arranged by size:
2109 1697 840 602 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4180 160
Size: 872 3148 1606
Fragments arranged by size:
3148 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5626
AvaI C'yCGr_G
Cuts at: 1870 1943 2103 2163 2216 2951 1870
Size: 73 160 60 53 735 4545
Fragments arranged by size:
4545 735 160 73 60 53
BamHI G'GATC_C
Cuts at: 2039 2114 2956 3323 2039
Size: 75 842 367 4342
Fragments arranged by size:
4342 842 367 75
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2032 2049 2947 4402 618
Size: 325 217 627 245 17 898 1455 1842
Fragments arranged by size:
1842 1455 898 627 325 245 217 17
BanII G_rGCy'C
Cuts at: 1391 2204 2945 1391
Size: 813 741 4072
Fragments arranged by size:
4072 813 741
BbeI G_GCGC'C
Cuts at: 2036 2053 2036
Size: 17 5609
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2324 928
Size: 971 425 4230
Fragments arranged by size:
4230 971 425
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3817 3851 1903
Size: 34 50 34 1796 34 3678
Fragments arranged by size:
3678 1796 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3518 4728 3518
Size: 1210 4416
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5626
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2165 4237 136
Size: 107 122 71 631 605 493 2072 1525
Fragments arranged by size:
2072 1525 631 605 493 122 107 71
BglII A'GATC_T
Cuts at: 5621 5621
Size: 5626
BlpI GC'TnA_GC
Cuts at: 3043 3043
Size: 5626
Bme1580I G_kGCm'C
Cuts at: 1790 3687 4843 1790
Size: 1897 1156 2573
Fragments arranged by size:
2573 1897 1156
BmgBI CAC'GTC
Cuts at: 1318 2650 1318
Size: 1332 4294
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2918 3357 4315
Size: 416 53 83 399 24 1501 439 958
Cuts at: 4315 442
Size: 1753
Fragments arranged by size:
1753 1501 958 439 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5626
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2886 4286 1239
Size: 1647 1400 2579
Fragments arranged by size:
2579 1647 1400
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2656 3751 4616 4914 2656
Size: 1095 865 298 3368
Fragments arranged by size:
3368 1095 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2021 4289 882
Size: 617 522 2268 2219
Fragments arranged by size:
2268 2219 617 522
BsaAI yAC'GTr
Cuts at: 493 1204 3417 493
Size: 711 2213 2702
Fragments arranged by size:
2702 2213 711
BsaBI GATnn'nnATC
Cuts at: 5620 5620
Size: 5626
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2033 2050 2355 3433
Size: 53 83 186 936 500 17 305 1078
Cuts at: 3433 3815 4450 275
Size: 382 635 1451
Fragments arranged by size:
1451 1078 936 635 500 382 305 186
83 53 17
BsaWI w'CCGG_w
Cuts at: 4059 4457 4731 4878 5289 4059
Size: 398 274 147 411 4396
Fragments arranged by size:
4396 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2363 2393 2846 2876 1178
Size: 30 165 30 960 30 453 30 3928
Fragments arranged by size:
3928 960 453 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2744 2795 1186
Size: 1558 51 4017
Fragments arranged by size:
4017 1558 51
BseYI C'CCAG_C
Cuts at: 2154 2442 4829 5325 2154
Size: 288 2387 496 2455
Fragments arranged by size:
2455 2387 496 288
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2252 2489 1163
Size: 573 516 237 4300
Fragments arranged by size:
4300 573 516 237
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2012 3076 3837 4865 1540
Size: 275 186 11 1064 761 1028 2301
Fragments arranged by size:
2301 1064 1028 761 275 186 11
BsiHKAI G_wGCw'C
Cuts at: 3687 3772 4843 3687
Size: 85 1071 4470
Fragments arranged by size:
4470 1071 85
BsmI GAATG_Cn'
Cuts at: 3137 3230 3137
Size: 93 5533
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2204 2945 3687 3772 4843 1391
Size: 399 414 741 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 741 414 399 85
BspEI T'CCGG_A
Cuts at: 5289 5289
Size: 5626
BspHI T'CATG_A
Cuts at: 3515 5245 3515
Size: 1730 3896
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2981 844
Size: 2137 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2120 3513 1394
Size: 429 297 1393 3507
Fragments arranged by size:
3507 1393 429 297
BsrDI GCAATG_nn'
Cuts at: 65 1248 2917 4121 4295 65
Size: 1183 1669 1204 174 1396
Fragments arranged by size:
1669 1396 1204 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4270 4457 1700
Size: 110 2460 187 2869
Fragments arranged by size:
2869 2460 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5626
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5626
BssSI C'ACGA_G
Cuts at: 3686 4698 3686
Size: 1012 4614
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5626
BstBI TT'CG_AA
Cuts at: 4430 4430
Size: 5626
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5626
BstYI r'GATC_y
Cuts at: 1972 2039 2114 2631 2956 3323 3708 3725 5177
Size: 67 75 517 325 367 385 17 1452
Cuts at: 5177 5263 5275 5621 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 517 385 367 346 325 86
75 67 17 12
Bsu36I CC'TnA_GG
Cuts at: 2209 2502 2209
Size: 293 5333
BtgI C'CryG_G
Cuts at: 513 1065 2222 2398 2811 513
Size: 552 1157 176 413 3328
Fragments arranged by size:
3328 1157 552 413 176
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4887
Fragments arranged by size:
4887 683 56
BtsI GCAGTG_nn'
Cuts at: 3142 3936 3956 3142
Size: 794 20 4812
Fragments arranged by size:
4812 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5626
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5591
DraI TTT'AAA
Cuts at: 2930 2930
Size: 5626
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4633 809
Size: 507 3317 1802
Fragments arranged by size:
3317 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2141 2401 3073
Size: 54 1425 50 275 18 311 260 672
Cuts at: 3073 3962 5591 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 672 311 275 260 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3073 1537
Size: 275 1261 4090
Fragments arranged by size:
4090 1261 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2708 2721 3556 1353
Size: 1355 13 835 3423
Fragments arranged by size:
3423 1355 835 13
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4213 4587 4733 387
Size: 1064 138 2624 374 146 1280
Fragments arranged by size:
2624 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2886 3689 4286 5073 5341 1239
Size: 393 1254 803 597 787 268 1524
Fragments arranged by size:
1524 1254 803 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2213 2213
Size: 5626
EcoRI G'AATT_C
Cuts at: 2333 2935 3313 2333
Size: 602 378 4646
Fragments arranged by size:
4646 602 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2819 2819
Size: 5626
FspI TGC'GCA
Cuts at: 4132 4132
Size: 5626
HaeII r_GCGC'y
Cuts at: 1678 2036 2053 2171 2239 4773 1678
Size: 358 17 118 68 2534 2531
Fragments arranged by size:
2534 2531 358 118 68 17
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2001 2033 2055 2087 2264
Size: 32 135 32 429 32 22 32 177
Cuts at: 2264 2296 2363 2395 4267 4299 4341 4373 1373
Size: 32 67 32 1872 32 42 32 2626
Fragments arranged by size:
2626 1872 429 177 135 67 42 32
32 32 32 32 32 32 32 22
HincII GTy'rAC
Cuts at: 669 1657 1938 2367 2970 3216 669
Size: 988 281 429 603 246 3079
Fragments arranged by size:
3079 988 603 429 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5626
HpaI GTT'AAC
Cuts at: 2367 3216 2367
Size: 849 4777
KasI G'GCGC_C
Cuts at: 2032 2049 2032
Size: 17 5609
KpnI G_GTAC'C
Cuts at: 2951 2951
Size: 5626
MfeI C'AATT_G
Cuts at: 3225 3225
Size: 5626
MluI A'CGCG_T
Cuts at: 5502 5502
Size: 5626
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2367 2595 4740 4924 1305
Size: 1062 228 2145 184 2007
Fragments arranged by size:
2145 2007 1062 228 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2403 10
Size: 54 1425 343 571 3233
Fragments arranged by size:
3233 1425 571 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2648 2775 3584 3943 4102 518
Size: 648 1482 127 809 359 159 2042
Fragments arranged by size:
2042 1482 809 648 359 159 127
MspA1I CmG'CkG
Cuts at: 1606 2146 2224 2746 3042 3719 4867 5112 1606
Size: 540 78 522 296 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 540 522 296 245 78
NarI GG'CG_CC
Cuts at: 2033 2050 2033
Size: 17 5609
NcoI C'CATG_G
Cuts at: 513 1065 2811 513
Size: 552 1746 3328
Fragments arranged by size:
3328 1746 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5626
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5626
NotI GC'GGCC_GC
Cuts at: 3073 3073
Size: 5626
NspI r_CATG'y
Cuts at: 1132 1229 2328 2984 4529 1132
Size: 97 1099 656 1545 2229
Fragments arranged by size:
2229 1545 1099 656 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5626
PciI A'CATG_T
Cuts at: 4525 4525
Size: 5626
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2060 2851 1108
Size: 952 791 3883
Fragments arranged by size:
3883 952 791
PmeI GTTT'AAAC
Cuts at: 2930 2930
Size: 5626
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3695 3727 5208 5240 3695
Size: 32 1481 32 4081
Fragments arranged by size:
4081 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2354 1613
Size: 741 4885
PsiI TTA'TAA
Cuts at: 3196 3196
Size: 5626
PstI C_TGCA'G
Cuts at: 830 2978 830
Size: 2148 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5626
SacII CC_GC'GG
Cuts at: 2225 2225
Size: 5626
SalI G'TCGA_C
Cuts at: 1936 2968 1936
Size: 1032 4594
SbfI CC_TGCA'GG
Cuts at: 2978 2978
Size: 5626
ScaI AGT'ACT
Cuts at: 3874 5586 3874
Size: 1712 3914
SfcI C'TryA_G
Cuts at: 826 1045 2456 2974 4109 4790 4981 826
Size: 219 1411 518 1135 681 191 1471
Fragments arranged by size:
1471 1411 1135 681 518 219 191
SfoI GGC'GCC
Cuts at: 2034 2051 2034
Size: 17 5609
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5626
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5626
SmaI CCC'GGG
Cuts at: 1872 2105 2165 2218 2953 1872
Size: 233 60 53 735 4545
Fragments arranged by size:
4545 735 233 60 53
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2635 3730 4631 4893 820
Size: 197 926 692 1095 901 262 1553
Fragments arranged by size:
1553 1095 926 901 692 262 197
SnaBI TAC'GTA
Cuts at: 493 3417 493
Size: 2924 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5626
SphI G_CATG'C
Cuts at: 1132 2984 1132
Size: 1852 3774
SrfI GCCC'GGGC
Cuts at: 2165 2165
Size: 5626
SspI AAT'ATT
Cuts at: 5 52 2305 2895 3550 5
Size: 47 2253 590 655 2081
Fragments arranged by size:
2253 2081 655 590 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5626
StyI C'CwwG_G
Cuts at: 513 1065 2811 3065 513
Size: 552 1746 254 3074
Fragments arranged by size:
3074 1746 552 254
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3653 3670 3823 4008 4453 325
Size: 1255 2073 17 153 185 445 1498
Fragments arranged by size:
2073 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3872 5584 96
Size: 274 80 33 51 1443 1895 1712 138
Fragments arranged by size:
1895 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5586 5618 5586
Size: 32 5594
Tth111I GACn'n_nGTC
Cuts at: 1316 2013 1316
Size: 697 4929
XbaI T'CTAG_A
Cuts at: 2962 2962
Size: 5626
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5626
XmaI C'CCGG_G
Cuts at: 1870 2103 2163 2216 2951 1870
Size: 233 60 53 735 4545
Fragments arranged by size:
4545 735 233 60 53
XmnI GAAnn'nnTTC
Cuts at: 2763 3755 2763
Size: 992 4634
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2356 3434 276
Size: 53 83 186 936 822 1078 2468
Fragments arranged by size:
2468 1078 936 822 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI SacII SalI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII
EcoICRI EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI
NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacI SanDI SapI SexAI SfiI SwaI
XcmI