Restriction map for
pFN21AE3119
(Circular) (Six-base) MAPSORT of: pFN21AE3119.seq Check: 8227 from: 1 to: 5584
pFN21AE3119 5584 bp
With 182 enzymes: SgfI *
December 28, 2011 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3394 278
Size: 53 83 186 936 1858 2468
Fragments arranged by size:
2468 1858 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2927 1937
Size: 990 4594
Acc65I G'GTAC_C
Cuts at: 2905 2905
Size: 5584
AclI AA'CG_TT
Cuts at: 1563 3711 4084 1563
Size: 2148 373 3063
Fragments arranged by size:
3063 2148 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2307 3647 5031 5299 1632
Size: 675 1340 1384 268 1917
Fragments arranged by size:
1917 1384 1340 675 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5387
AflIII A'CryG_T
Cuts at: 1203 4483 5460 1203
Size: 3280 977 1327
Fragments arranged by size:
3280 1327 977
AgeI A'CCGG_T
Cuts at: 4415 4415
Size: 5584
AhdI GACnn_n'nnGTC
Cuts at: 4313 4313
Size: 5584
AleI CACnn'nnGTG
Cuts at: 1166 2084 1166
Size: 918 4666
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2195 1184
Size: 1011 4573
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2786 2818 2786
Size: 32 5552
AlwNI CAG_nnn'CTG
Cuts at: 2104 2133 3327 4899 2104
Size: 29 1194 1572 2789
Fragments arranged by size:
2789 1572 1194 29
ApaI G_GGCC'C
Cuts at: 2748 2748
Size: 5584
ApaLI G'TGCA_C
Cuts at: 3641 4797 3641
Size: 1156 4428
ApoI r'AATT_y
Cuts at: 1493 2893 3119 3271 5380 1493
Size: 1400 226 152 2109 1697
Fragments arranged by size:
2109 1697 1400 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4138 160
Size: 872 3106 1606
Fragments arranged by size:
3106 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5584
AvaI C'yCGr_G
Cuts at: 1870 1943 2054 2676 2748 2909 1870
Size: 73 111 622 72 161 4545
Fragments arranged by size:
4545 622 161 111 73 72
BamHI G'GATC_C
Cuts at: 2914 3281 2914
Size: 367 5217
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2905 4360 618
Size: 325 217 627 1118 1455 1842
Fragments arranged by size:
1842 1455 1118 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2362 2604 2748 2903 1391
Size: 971 242 144 155 4072
Fragments arranged by size:
4072 971 242 155 144
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4613
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2752 2786 3775 3809 1903
Size: 34 815 34 989 34 3678
Fragments arranged by size:
3678 989 815 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3476 4686 3476
Size: 1210 4374
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5584
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4195 136
Size: 107 122 71 631 605 2523 1525
Fragments arranged by size:
2523 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5579 5579
Size: 5584
BlpI GC'TnA_GC
Cuts at: 2814 3001 2814
Size: 187 5397
Bme1580I G_kGCm'C
Cuts at: 1790 2091 2748 3645 4801 1790
Size: 301 657 897 1156 2573
Fragments arranged by size:
2573 1156 897 657 301
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5584
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2215 2473 2716
Size: 416 53 83 399 24 798 258 243
Cuts at: 2716 2737 3315 4273 442
Size: 21 578 958 1753
Fragments arranged by size:
1753 958 798 578 416 399 258 243
83 53 24 21
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5584
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2090 2376 4244 1239
Size: 851 286 1868 2579
Fragments arranged by size:
2579 1868 851 286
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2411 3709 4574 4872 2411
Size: 1298 865 298 3123
Fragments arranged by size:
3123 1298 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2656 4247 882
Size: 617 1157 1591 2219
Fragments arranged by size:
2219 1591 1157 617
BsaAI yAC'GTr
Cuts at: 493 1204 2086 3375 493
Size: 711 882 1289 2702
Fragments arranged by size:
2702 1289 882 711
BsaBI GATnn'nnATC
Cuts at: 5578 5578
Size: 5584
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3391 3773 4408 275
Size: 53 83 186 936 1858 382 635 1451
Fragments arranged by size:
1858 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4017 4415 4689 4836 5247 4017
Size: 398 274 147 411 4354
Fragments arranged by size:
4354 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2786 2816 1178
Size: 30 165 30 1383 30 3946
Fragments arranged by size:
3946 1383 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2483 2794 2855 1186
Size: 1297 311 61 3915
Fragments arranged by size:
3915 1297 311 61
BseYI C'CCAG_C
Cuts at: 2433 4787 5283 2433
Size: 2354 496 2734
Fragments arranged by size:
2734 2354 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2524 2579 1163
Size: 573 788 55 4168
Fragments arranged by size:
4168 788 573 55
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2492 3034 3795 4823 1540
Size: 275 186 491 542 761 1028 2301
Fragments arranged by size:
2301 1028 761 542 491 275 186
BsiHKAI G_wGCw'C
Cuts at: 3645 3730 4801 3645
Size: 85 1071 4428
Fragments arranged by size:
4428 1071 85
BsmI GAATG_Cn'
Cuts at: 3095 3188 3095
Size: 93 5491
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2091 2362 2604 2748 2903 3645 3730
Size: 399 301 271 242 144 155 742 85
Cuts at: 3730 4801 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 301 271 242 155
144 85
BspEI T'CCGG_A
Cuts at: 5247 5247
Size: 5584
BspHI T'CATG_A
Cuts at: 2154 3473 5203 2154
Size: 1319 1730 2535
Fragments arranged by size:
2535 1730 1319
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2939 844
Size: 2095 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3471 1394
Size: 429 1648 3507
Fragments arranged by size:
3507 1648 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4079 4253 65
Size: 1183 2831 174 1396
Fragments arranged by size:
2831 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4228 4415 1700
Size: 110 2418 187 2869
Fragments arranged by size:
2869 2418 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5584
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5584
BssSI C'ACGA_G
Cuts at: 3644 4656 3644
Size: 1012 4572
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5584
BstBI TT'CG_AA
Cuts at: 4388 4388
Size: 5584
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5584
BstYI r'GATC_y
Cuts at: 1972 2914 3281 3666 3683 5135 5221 5233 5579
Size: 942 367 385 17 1452 86 12 346
Cuts at: 5579 1972
Size: 1977
Fragments arranged by size:
1977 1452 942 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2217 2362 513
Size: 552 939 213 145 3735
Fragments arranged by size:
3735 939 552 213 145
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4845
Fragments arranged by size:
4845 683 56
BtsI GCAGTG_nn'
Cuts at: 3100 3894 3914 3100
Size: 794 20 4770
Fragments arranged by size:
4770 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5584
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5549
DraI TTT'AAA
Cuts at: 2888 2888
Size: 5584
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2387 4591 809
Size: 507 1071 2204 1802
Fragments arranged by size:
2204 1802 1071 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2220 2489 3031
Size: 54 1425 50 275 18 390 269 542
Cuts at: 3031 3920 5549 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 542 390 275 269 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2489 3031 1537
Size: 275 677 542 4090
Fragments arranged by size:
4090 677 542 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2283 2702 3514 1353
Size: 930 419 812 3423
Fragments arranged by size:
3423 930 812 419
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4171 4545 4691 387
Size: 1064 138 2582 374 146 1280
Fragments arranged by size:
2582 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2090 2307 2376 3647 4244 5031 5299
Size: 393 458 217 69 1271 597 787 268
Cuts at: 5299 1239
Size: 1524
Fragments arranged by size:
1524 1271 787 597 458 393 268 217
69
EcoNI CCTnn'n_nnAGG
Cuts at: 2183 2183
Size: 5584
EcoO109I rG'GnC_Cy
Cuts at: 2140 2745 2140
Size: 605 4979
EcoRI G'AATT_C
Cuts at: 2893 3271 2893
Size: 378 5206
FspI TGC'GCA
Cuts at: 2273 4090 2273
Size: 1817 3767
HaeII r_GCGC'y
Cuts at: 1678 4731 1678
Size: 3053 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2856 2888 4225 4257 4299
Size: 32 135 32 1284 32 1337 32 42
Cuts at: 4299 4331 1373
Size: 32 2626
Fragments arranged by size:
2626 1337 1284 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2928 3174 669
Size: 988 281 990 246 3079
Fragments arranged by size:
3079 990 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5584
HpaI GTT'AAC
Cuts at: 3174 3174
Size: 5584
KpnI G_GTAC'C
Cuts at: 2909 2909
Size: 5584
MfeI C'AATT_G
Cuts at: 3183 3183
Size: 5584
MluI A'CGCG_T
Cuts at: 5460 5460
Size: 5584
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2644 4698 4882 1305
Size: 1339 2054 184 2007
Fragments arranged by size:
2054 2007 1339 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3762
Fragments arranged by size:
3762 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2084 3542 3901 4060 518
Size: 648 918 1458 359 159 2042
Fragments arranged by size:
2042 1458 918 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2101 2334 2437 2563 3000 3677 4825 5070
Size: 495 233 103 126 437 677 1148 245
Cuts at: 5070 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 495 437 245 233 126
103
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4093
Fragments arranged by size:
4093 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5584
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5584
NotI GC'GGCC_GC
Cuts at: 3031 3031
Size: 5584
NspI r_CATG'y
Cuts at: 1132 1229 2942 4487 1132
Size: 97 1713 1545 2229
Fragments arranged by size:
2229 1713 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5584
PciI A'CATG_T
Cuts at: 4483 4483
Size: 5584
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2205 2218 2440 1108
Size: 1097 13 222 4252
Fragments arranged by size:
4252 1097 222 13
PmeI GTTT'AAAC
Cuts at: 2888 2888
Size: 5584
PmlI CAC'GTG
Cuts at: 2086 2086
Size: 5584
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2786 2818 3653 3685 5166 5198 2786
Size: 32 835 32 1481 32 3172
Fragments arranged by size:
3172 1481 835 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2140 2140
Size: 5584
PshAI GACnn'nnGTC
Cuts at: 1613 2648 1613
Size: 1035 4549
PsiI TTA'TAA
Cuts at: 3154 3154
Size: 5584
PspOMI G'GGCC_C
Cuts at: 2744 2744
Size: 5584
PstI C_TGCA'G
Cuts at: 830 2129 2936 830
Size: 1299 807 3478
Fragments arranged by size:
3478 1299 807
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5584
PvuII CAG'CTG
Cuts at: 2101 2437 2563 2101
Size: 336 126 5122
Fragments arranged by size:
5122 336 126
SalI G'TCGA_C
Cuts at: 1936 2926 1936
Size: 990 4594
SbfI CC_TGCA'GG
Cuts at: 2936 2936
Size: 5584
ScaI AGT'ACT
Cuts at: 2726 3832 5544 2726
Size: 1106 1712 2766
Fragments arranged by size:
2766 1712 1106
SfcI C'TryA_G
Cuts at: 826 1045 2125 2932 4067 4748 4939 826
Size: 219 1080 807 1135 681 191 1471
Fragments arranged by size:
1471 1135 1080 807 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5584
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5584
SmaI CCC'GGG
Cuts at: 1872 2678 2911 1872
Size: 806 233 4545
Fragments arranged by size:
4545 806 233
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2426 3688 4589 4851 820
Size: 197 926 483 1262 901 262 1553
Fragments arranged by size:
1553 1262 926 901 483 262 197
SnaBI TAC'GTA
Cuts at: 493 3375 493
Size: 2882 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5584
SphI G_CATG'C
Cuts at: 1132 2942 1132
Size: 1810 3774
SspI AAT'ATT
Cuts at: 5 52 3508 5
Size: 47 3456 2081
Fragments arranged by size:
3456 2081 47
StuI AGG'CCT
Cuts at: 1517 2801 1517
Size: 1284 4300
StyI C'CwwG_G
Cuts at: 513 1065 2004 2199 3023 513
Size: 552 939 195 824 3074
Fragments arranged by size:
3074 939 824 552 195
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2735 3611 3628 3781 3966 4411 325
Size: 1255 1155 876 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1155 876 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2724 3830 5542
Size: 274 80 33 51 1443 747 1106 1712
Cuts at: 5542 96
Size: 138
Fragments arranged by size:
1712 1443 1106 747 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5544 5576 5544
Size: 32 5552
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5584
XbaI T'CTAG_A
Cuts at: 2920 2920
Size: 5584
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2029 2029
Size: 5584
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5584
XmaI C'CCGG_G
Cuts at: 1870 2676 2909 1870
Size: 806 233 4545
Fragments arranged by size:
4545 806 233
XmnI GAAnn'nnTTC
Cuts at: 2234 3713 2234
Size: 1479 4105
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3392 276
Size: 53 83 186 936 1858 2468
Fragments arranged by size:
2468 1858 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PspXI PsrI RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI