Restriction map for
pFN21AE3113
(Circular) (Six-base) MAPSORT of: pFN21AE3113.seq Check: 9833 from: 1 to: 5563
pFN21AE3113 5563 bp
With 182 enzymes: SgfI *
December 28, 2011 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3373 278
Size: 53 83 186 936 1837 2468
Fragments arranged by size:
2468 1837 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2373 2906 1937
Size: 436 533 4594
Fragments arranged by size:
4594 533 436
Acc65I G'GTAC_C
Cuts at: 2202 2884 2202
Size: 682 4881
AclI AA'CG_TT
Cuts at: 1563 3690 4063 1563
Size: 2127 373 3063
Fragments arranged by size:
3063 2127 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2329 3626 5010 5278 1632
Size: 697 1297 1384 268 1917
Fragments arranged by size:
1917 1384 1297 697 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5366
AflIII A'CryG_T
Cuts at: 1203 4462 5439 1203
Size: 3259 977 1327
Fragments arranged by size:
3259 1327 977
AgeI A'CCGG_T
Cuts at: 4394 4394
Size: 5563
AhdI GACnn_n'nnGTC
Cuts at: 4292 4292
Size: 5563
AleI CACnn'nnGTG
Cuts at: 1166 2825 1166
Size: 1659 3904
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5563
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2129 2161 2129
Size: 32 5531
AlwNI CAG_nnn'CTG
Cuts at: 2804 3306 4878 2804
Size: 502 1572 3489
Fragments arranged by size:
3489 1572 502
ApaI G_GGCC'C
Cuts at: 2495 2539 2495
Size: 44 5519
ApaLI G'TGCA_C
Cuts at: 3620 4776 3620
Size: 1156 4407
ApoI r'AATT_y
Cuts at: 1493 2691 2872 3098 3250 5359 1493
Size: 1198 181 226 152 2109 1697
Fragments arranged by size:
2109 1697 1198 226 181 152
AseI AT'TA_AT
Cuts at: 160 1032 4117 160
Size: 872 3085 1606
Fragments arranged by size:
3085 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5563
AvaI C'yCGr_G
Cuts at: 1870 1943 2020 2038 2086 2888 1870
Size: 73 77 18 48 802 4545
Fragments arranged by size:
4545 802 77 73 48 18
BamHI G'GATC_C
Cuts at: 2893 3260 2893
Size: 367 5196
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2011 2202 2884 4339 618
Size: 325 217 627 224 191 682 1455 1842
Fragments arranged by size:
1842 1455 682 627 325 224 217 191
BanII G_rGCy'C
Cuts at: 1391 2087 2093 2495 2539 2882 1391
Size: 696 6 402 44 343 4072
Fragments arranged by size:
4072 696 402 343 44 6
BbeI G_GCGC'C
Cuts at: 2015 2015
Size: 5563
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4592
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3754 3788 1903
Size: 34 1817 34 3678
Fragments arranged by size:
3678 1817 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3455 4665 3455
Size: 1210 4353
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5563
BfrBI ATG'CAT
Cuts at: 2471 2471
Size: 5563
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2024 2105
Size: 107 122 71 631 605 337 15 81
Cuts at: 2105 2217 2499 4174 136
Size: 112 282 1675 1525
Fragments arranged by size:
1675 1525 631 605 337 282 122 112
107 81 71 15
BglII A'GATC_T
Cuts at: 5558 5558
Size: 5563
BlpI GC'TnA_GC
Cuts at: 2980 2980
Size: 5563
Bme1580I G_kGCm'C
Cuts at: 1790 2495 2504 2539 3624 4780 1790
Size: 705 9 35 1085 1156 2573
Fragments arranged by size:
2573 1156 1085 705 35 9
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5563
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3294 4252 442
Size: 416 53 83 399 24 1877 958 1753
Fragments arranged by size:
1877 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5563
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2156 2177 2347 4223 1239
Size: 917 21 170 1876 2579
Fragments arranged by size:
2579 1876 917 170 21
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3688 4553 4851 3688
Size: 865 298 4400
Fragments arranged by size:
4400 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4226 882
Size: 617 2727 2219
Fragments arranged by size:
2727 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2327 3354 493
Size: 711 1123 1027 2702
Fragments arranged by size:
2702 1123 1027 711
BsaBI GATnn'nnATC
Cuts at: 5557 5557
Size: 5563
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2012 3370 3752 4387
Size: 53 83 186 936 479 1358 382 635
Cuts at: 4387 275
Size: 1451
Fragments arranged by size:
1451 1358 936 635 479 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3996 4394 4668 4815 5226 3996
Size: 398 274 147 411 4333
Fragments arranged by size:
4333 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2129 2159 1178
Size: 30 165 30 726 30 4582
Fragments arranged by size:
4582 726 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5563
BseYI C'CCAG_C
Cuts at: 2027 2123 2306 2569 2589 2794 4766 5262 2027
Size: 96 183 263 20 205 1972 496 2328
Fragments arranged by size:
2328 1972 496 263 205 183 96 20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2198 1163
Size: 573 462 4528
Fragments arranged by size:
4528 573 462
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3013 3774 4802 1540
Size: 275 186 1012 761 1028 2301
Fragments arranged by size:
2301 1028 1012 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2426 3624 3709 4780 2426
Size: 1198 85 1071 3209
Fragments arranged by size:
3209 1198 1071 85
BsmI GAATG_Cn'
Cuts at: 3074 3167 3074
Size: 93 5470
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2087 2093 2426 2495 2504 2539 2882
Size: 399 297 6 333 69 9 35 343
Cuts at: 2882 3624 3709 4780 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 343 333 297 85
69 35 9 6
BspEI T'CCGG_A
Cuts at: 5226 5226
Size: 5563
BspHI T'CATG_A
Cuts at: 3452 5182 3452
Size: 1730 3833
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2310 2918 844
Size: 1466 608 3489
Fragments arranged by size:
3489 1466 608
BsrBI CCG'CTC
Cuts at: 1394 1823 3450 1394
Size: 429 1627 3507
Fragments arranged by size:
3507 1627 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2412 2564 4058 4232 65
Size: 1183 1164 152 1494 174 1396
Fragments arranged by size:
1494 1396 1183 1164 174 152
BsrFI r'CCGG_y
Cuts at: 1700 1810 2014 2115 4207 4394 1700
Size: 110 204 101 2092 187 2869
Fragments arranged by size:
2869 2092 204 187 110 101
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5563
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5563
BssSI C'ACGA_G
Cuts at: 3623 4635 3623
Size: 1012 4551
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2804 794
Size: 2010 3553
BstBI TT'CG_AA
Cuts at: 4367 4367
Size: 5563
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5563
BstYI r'GATC_y
Cuts at: 1972 2893 3260 3645 3662 5114 5200 5212 5558
Size: 921 367 385 17 1452 86 12 346
Cuts at: 5558 1972
Size: 1977
Fragments arranged by size:
1977 1452 921 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2100 513
Size: 552 939 96 3976
Fragments arranged by size:
3976 939 552 96
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2235 487
Size: 683 56 1009 3815
Fragments arranged by size:
3815 1009 683 56
BtsI GCAGTG_nn'
Cuts at: 2517 2552 2581 3079 3873 3893 2517
Size: 35 29 498 794 20 4187
Fragments arranged by size:
4187 794 498 35 29 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5563
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5528
DraI TTT'AAA
Cuts at: 2867 2867
Size: 5563
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4570 809
Size: 507 3254 1802
Fragments arranged by size:
3254 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3010 3899 5528
Size: 54 1425 50 275 18 1180 889 1629
Cuts at: 5528 8
Size: 43
Fragments arranged by size:
1629 1425 1180 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3010 1537
Size: 275 1198 4090
Fragments arranged by size:
4090 1198 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2265 3493 1353
Size: 912 1228 3423
Fragments arranged by size:
3423 1228 912
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2257 4150 4524 4670 387
Size: 1064 138 668 1893 374 146 1280
Fragments arranged by size:
1893 1280 1064 668 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2156 2177 2329 2347 3626 4223 5010
Size: 393 524 21 152 18 1279 597 787
Cuts at: 5010 5278 1239
Size: 268 1524
Fragments arranged by size:
1524 1279 787 597 524 393 268 152
21 18
EcoO109I rG'GnC_Cy
Cuts at: 2491 2492 2536 2491
Size: 1 44 5518
Fragments arranged by size:
5518 44 1
EcoRI G'AATT_C
Cuts at: 2691 2872 3250 2691
Size: 181 378 5004
Fragments arranged by size:
5004 378 181
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2407 2803 2407
Size: 396 5167
FspI TGC'GCA
Cuts at: 2282 4069 2282
Size: 1787 3776
HaeII r_GCGC'y
Cuts at: 1678 2015 2049 4710 1678
Size: 337 34 2661 2531
Fragments arranged by size:
2661 2531 337 34
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4204 4236 4278 4310 1373
Size: 32 135 32 2632 32 42 32 2626
Fragments arranged by size:
2632 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2907 3153 669
Size: 988 281 969 246 3079
Fragments arranged by size:
3079 988 969 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5563
HpaI GTT'AAC
Cuts at: 3153 3153
Size: 5563
KasI G'GCGC_C
Cuts at: 2011 2011
Size: 5563
KpnI G_GTAC'C
Cuts at: 2206 2888 2206
Size: 682 4881
MfeI C'AATT_G
Cuts at: 3162 3162
Size: 5563
MluI A'CGCG_T
Cuts at: 5439 5439
Size: 5563
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4677 4861 1305
Size: 3372 184 2007
Fragments arranged by size:
3372 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3741
Fragments arranged by size:
3741 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2220 2825 3521 3880 4039 518
Size: 648 1054 605 696 359 159 2042
Fragments arranged by size:
2042 1054 696 648 605 359 159
MspA1I CmG'CkG
Cuts at: 1606 2102 2208 2979 3656 4804 5049 1606
Size: 496 106 771 677 1148 245 2120
Fragments arranged by size:
2120 1148 771 677 496 245 106
NaeI GCC'GGC
Cuts at: 2016 2117 2016
Size: 101 5462
NarI GG'CG_CC
Cuts at: 2012 2012
Size: 5563
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4072
Fragments arranged by size:
4072 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5563
NgoMIV G'CCGG_C
Cuts at: 2014 2115 2014
Size: 101 5462
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5563
NotI GC'GGCC_GC
Cuts at: 3010 3010
Size: 5563
NsiI A_TGCA'T
Cuts at: 2473 2473
Size: 5563
NspI r_CATG'y
Cuts at: 1132 1229 2342 2921 4466 1132
Size: 97 1113 579 1545 2229
Fragments arranged by size:
2229 1545 1113 579 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5563
PciI A'CATG_T
Cuts at: 4462 4462
Size: 5563
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5563
PfoI T'CCnGG_A
Cuts at: 2363 2363
Size: 5563
PmeI GTTT'AAAC
Cuts at: 2867 2867
Size: 5563
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2129 2161 3632 3664 5145 5177 2129
Size: 32 1471 32 1481 32 2515
Fragments arranged by size:
2515 1481 1471 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5563
PsiI TTA'TAA
Cuts at: 3133 3133
Size: 5563
PspOMI G'GGCC_C
Cuts at: 2491 2535 2491
Size: 44 5519
PstI C_TGCA'G
Cuts at: 830 2915 830
Size: 2085 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5563
SacII CC_GC'GG
Cuts at: 2103 2103
Size: 5563
SalI G'TCGA_C
Cuts at: 1936 2905 1936
Size: 969 4594
SbfI CC_TGCA'GG
Cuts at: 2915 2915
Size: 5563
ScaI AGT'ACT
Cuts at: 3811 5523 3811
Size: 1712 3851
SfcI C'TryA_G
Cuts at: 826 1045 2053 2911 4046 4727 4918 826
Size: 219 1008 858 1135 681 191 1471
Fragments arranged by size:
1471 1135 1008 858 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2105 2105
Size: 5563
SfoI GGC'GCC
Cuts at: 2013 2013
Size: 5563
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5563
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5563
SmaI CCC'GGG
Cuts at: 1872 2022 2890 1872
Size: 150 868 4545
Fragments arranged by size:
4545 868 150
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3667 4568 4830 820
Size: 197 926 1724 901 262 1553
Fragments arranged by size:
1724 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3354 493
Size: 2861 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5563
SphI G_CATG'C
Cuts at: 1132 2921 1132
Size: 1789 3774
SspI AAT'ATT
Cuts at: 5 52 3487 5
Size: 47 3435 2081
Fragments arranged by size:
3435 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5563
StyI C'CwwG_G
Cuts at: 513 1065 2004 2551 2842 3002 513
Size: 552 939 547 291 160 3074
Fragments arranged by size:
3074 939 552 547 291 160
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3590 3607 3760 3945 4390 325
Size: 1255 2010 17 153 185 445 1498
Fragments arranged by size:
2010 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3809 5521 96
Size: 274 80 33 51 1443 1832 1712 138
Fragments arranged by size:
1832 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2279 2311 2468 2500 5523 5555 2279
Size: 32 157 32 3023 32 2287
Fragments arranged by size:
3023 2287 157 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5563
XbaI T'CTAG_A
Cuts at: 2899 2899
Size: 5563
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2131 2131
Size: 5563
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5563
XmaI C'CCGG_G
Cuts at: 1870 2020 2888 1870
Size: 150 868 4545
Fragments arranged by size:
4545 868 150
XmnI GAAnn'nnTTC
Cuts at: 2691 3692 2691
Size: 1001 4562
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3371 276
Size: 53 83 186 936 1837 2468
Fragments arranged by size:
2468 1837 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI
SacII SalI SbfI ScaI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbvCI BplI Bpu10I
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FseI FspAI NruI PacI PmlI PpuMI PspXI
PsrI PvuII RsrII SacI SanDI SapI SexAI SrfI
SwaI