Restriction map for
pFN21AE3110
(Circular) (Six-base) MAPSORT of: pFN21AE3110.seq Check: 6255 from: 1 to: 5548
pFN21AE3110 5548 bp
With 182 enzymes: SgfI *
December 28, 2011 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3358 278
Size: 53 83 186 936 1822 2468
Fragments arranged by size:
2468 1822 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2891 1937
Size: 954 4594
Acc65I G'GTAC_C
Cuts at: 2869 2869
Size: 5548
AclI AA'CG_TT
Cuts at: 1563 3675 4048 1563
Size: 2112 373 3063
Fragments arranged by size:
3063 2112 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2227 3611 4995 5263 1632
Size: 595 1384 1384 268 1917
Fragments arranged by size:
1917 1384 1384 595 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5351
AflIII A'CryG_T
Cuts at: 1203 2211 4447 5424 1203
Size: 1008 2236 977 1327
Fragments arranged by size:
2236 1327 1008 977
AgeI A'CCGG_T
Cuts at: 4379 4379
Size: 5548
AhdI GACnn_n'nnGTC
Cuts at: 4277 4277
Size: 5548
AleI CACnn'nnGTG
Cuts at: 1166 2618 1166
Size: 1452 4096
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2636 1184
Size: 1452 4096
AlwNI CAG_nnn'CTG
Cuts at: 3291 4863 3291
Size: 1572 3976
ApaLI G'TGCA_C
Cuts at: 2621 3605 4761 2621
Size: 984 1156 3408
Fragments arranged by size:
3408 1156 984
ApoI r'AATT_y
Cuts at: 1493 2857 3083 3235 5344 1493
Size: 1364 226 152 2109 1697
Fragments arranged by size:
2109 1697 1364 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4102 160
Size: 872 3070 1606
Fragments arranged by size:
3070 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5548
AvaI C'yCGr_G
Cuts at: 1870 1943 2873 1870
Size: 73 930 4545
Fragments arranged by size:
4545 930 73
BamHI G'GATC_C
Cuts at: 2271 2878 3245 2271
Size: 607 367 4574
Fragments arranged by size:
4574 607 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2869 4324 618
Size: 325 217 627 1082 1455 1842
Fragments arranged by size:
1842 1455 1082 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2012 2205 2810 2867 1391
Size: 621 193 605 57 4072
Fragments arranged by size:
4072 621 605 193 57
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4577
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2330 2364 3739 3773 1903
Size: 34 393 34 1375 34 3678
Fragments arranged by size:
3678 1375 393 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3440 4650 3440
Size: 1210 4338
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5548
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2741 4159 136
Size: 107 122 71 631 605 1069 1418 1525
Fragments arranged by size:
1525 1418 1069 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2550 2841 5543 2550
Size: 291 2702 2555
Fragments arranged by size:
2702 2555 291
BlpI GC'TnA_GC
Cuts at: 2965 2965
Size: 5548
Bme1580I G_kGCm'C
Cuts at: 1790 2625 3609 4765 1790
Size: 835 984 1156 2573
Fragments arranged by size:
2573 1156 984 835
BmgBI CAC'GTC
Cuts at: 1318 2077 2214 1318
Size: 759 137 4652
Fragments arranged by size:
4652 759 137
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3279 4237 442
Size: 416 53 83 399 24 1862 958 1753
Fragments arranged by size:
1862 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5548
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2117 2219 2239 2575 4208 1239
Size: 878 102 20 336 1633 2579
Fragments arranged by size:
2579 1633 878 336 102 20
Bpu10I CC'TnA_GC
Cuts at: 2265 2265
Size: 5548
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2033 2654 2759 3673 4538 4836 2033
Size: 621 105 914 865 298 2745
Fragments arranged by size:
2745 914 865 621 298 105
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4211 882
Size: 617 2712 2219
Fragments arranged by size:
2712 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3339 493
Size: 711 2135 2702
Fragments arranged by size:
2702 2135 711
BsaBI GATnn'nnATC
Cuts at: 5542 5542
Size: 5548
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3355 3737 4372 275
Size: 53 83 186 936 1822 382 635 1451
Fragments arranged by size:
1822 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2023 3981 4379 4653 4800 5211 2023
Size: 1958 398 274 147 411 2360
Fragments arranged by size:
2360 1958 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5323
Fragments arranged by size:
5323 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5548
BseYI C'CCAG_C
Cuts at: 4751 5247 4751
Size: 496 5052
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2218 2372 2596 1163
Size: 573 482 154 224 4115
Fragments arranged by size:
4115 573 482 224 154
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2185 2323 2719 2998 3759 4787
Size: 275 186 184 138 396 279 761 1028
Cuts at: 4787 1540
Size: 2301
Fragments arranged by size:
2301 1028 761 396 279 275 186 184
138
BsiHKAI G_wGCw'C
Cuts at: 2205 2529 2625 3609 3694 4765 2205
Size: 324 96 984 85 1071 2988
Fragments arranged by size:
2988 1071 984 324 96 85
BsiWI C'GTAC_G
Cuts at: 2130 2433 2130
Size: 303 5245
BsmI GAATG_Cn'
Cuts at: 3059 3152 3059
Size: 93 5455
BsmBI CGTCTCn'nnnn_
Cuts at: 2611 2611
Size: 5548
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2012 2205 2529 2625 2810 2867 3609
Size: 399 222 193 324 96 185 57 742
Cuts at: 3609 3694 4765 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 324 222 193 185
96 85 57
BspEI T'CCGG_A
Cuts at: 5211 5211
Size: 5548
BspHI T'CATG_A
Cuts at: 2706 3437 5167 2706
Size: 731 1730 3087
Fragments arranged by size:
3087 1730 731
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2052 2398 2903 844
Size: 1208 346 505 3489
Fragments arranged by size:
3489 1208 505 346
BsrBI CCG'CTC
Cuts at: 1394 1823 3435 1394
Size: 429 1612 3507
Fragments arranged by size:
3507 1612 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4043 4217 65
Size: 1183 2795 174 1396
Fragments arranged by size:
2795 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2184 2226 2382 4192 4379 1700
Size: 110 374 42 156 1810 187 2869
Fragments arranged by size:
2869 1810 374 187 156 110 42
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5548
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5548
BssSI C'ACGA_G
Cuts at: 3608 4620 3608
Size: 1012 4536
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5548
BstBI TT'CG_AA
Cuts at: 4352 4352
Size: 5548
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5548
BstXI CCAn_nnnn'nTGG
Cuts at: 2176 2176
Size: 5548
BstYI r'GATC_y
Cuts at: 1972 2091 2271 2367 2550 2841 2878 3245 3630
Size: 119 180 96 183 291 37 367 385
Cuts at: 3630 3647 5099 5185 5197 5543 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 385 367 346 291 183 180
119 96 86 37 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4057
Fragments arranged by size:
4057 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4809
Fragments arranged by size:
4809 683 56
BtsI GCAGTG_nn'
Cuts at: 2642 3064 3858 3878 2642
Size: 422 794 20 4312
Fragments arranged by size:
4312 794 422 20
ClaI AT'CG_AT
Cuts at: 1554 2340 1554
Size: 786 4762
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5513
DraI TTT'AAA
Cuts at: 2852 2852
Size: 5548
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4555 809
Size: 507 3239 1802
Fragments arranged by size:
3239 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2182 2685 2716
Size: 54 1425 50 275 18 352 503 31
Cuts at: 2716 2733 2995 3884 5513 8
Size: 17 262 889 1629 43
Fragments arranged by size:
1629 1425 889 503 352 275 262 54
50 43 31 18 17
EagI C'GGCC_G
Cuts at: 1537 1812 2182 2716 2995 1537
Size: 275 370 534 279 4090
Fragments arranged by size:
4090 534 370 279 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2024 3478 1353
Size: 671 1454 3423
Fragments arranged by size:
3423 1454 671
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4135 4509 4655 387
Size: 1064 138 2546 374 146 1280
Fragments arranged by size:
2546 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2203 2203
Size: 5548
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2117 2219 2227 2239 2575 3611 4208
Size: 393 485 102 8 12 336 1036 597
Cuts at: 4208 4995 5263 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1036 787 597 485 393 336 268
102 12 8
EcoNI CCTnn'n_nnAGG
Cuts at: 2254 2254
Size: 5548
EcoO109I rG'GnC_Cy
Cuts at: 2247 2410 2584 2247
Size: 163 174 5211
Fragments arranged by size:
5211 174 163
EcoRI G'AATT_C
Cuts at: 2857 3235 2857
Size: 378 5170
FspI TGC'GCA
Cuts at: 4054 4054
Size: 5548
HaeII r_GCGC'y
Cuts at: 1678 2265 4695 1678
Size: 587 2430 2531
Fragments arranged by size:
2531 2430 587
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4189 4221 4263 4295 1373
Size: 32 135 32 2617 32 42 32 2626
Fragments arranged by size:
2626 2617 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2892 3138 669
Size: 988 281 954 246 3079
Fragments arranged by size:
3079 988 954 281 246
HindIII A'AGCT_T
Cuts at: 748 2636 748
Size: 1888 3660
HpaI GTT'AAC
Cuts at: 3138 3138
Size: 5548
KpnI G_GTAC'C
Cuts at: 2873 2873
Size: 5548
MfeI C'AATT_G
Cuts at: 3147 3147
Size: 5548
MluI A'CGCG_T
Cuts at: 5424 5424
Size: 5548
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2356 4662 4846 1305
Size: 1051 2306 184 2007
Fragments arranged by size:
2306 2007 1051 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2687 2735 10
Size: 54 1425 343 855 48 2823
Fragments arranged by size:
2823 1425 855 343 54 48
MslI CAynn'nnrTG
Cuts at: 518 1166 2618 3506 3865 4024 518
Size: 648 1452 888 359 159 2042
Fragments arranged by size:
2042 1452 888 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2301 2325 2739 2964 3641 4789 5034 1606
Size: 695 24 414 225 677 1148 245 2120
Fragments arranged by size:
2120 1148 695 677 414 245 225 24
NaeI GCC'GGC
Cuts at: 2186 2186
Size: 5548
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4057
Fragments arranged by size:
4057 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5548
NgoMIV G'CCGG_C
Cuts at: 2184 2184
Size: 5548
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5548
NotI GC'GGCC_GC
Cuts at: 2995 2995
Size: 5548
NspI r_CATG'y
Cuts at: 1132 1229 2351 2906 4451 1132
Size: 97 1122 555 1545 2229
Fragments arranged by size:
2229 1545 1122 555 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5548
PciI A'CATG_T
Cuts at: 4447 4447
Size: 5548
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2301 1108
Size: 1193 4355
PfoI T'CCnGG_A
Cuts at: 2094 2094
Size: 5548
PmeI GTTT'AAAC
Cuts at: 2852 2852
Size: 5548
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3617 3649 5130 5162 3617
Size: 32 1481 32 4003
Fragments arranged by size:
4003 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2247 2410 2584 2247
Size: 163 174 5211
Fragments arranged by size:
5211 174 163
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5548
PsiI TTA'TAA
Cuts at: 3118 3118
Size: 5548
PstI C_TGCA'G
Cuts at: 830 2109 2583 2900 830
Size: 1279 474 317 3478
Fragments arranged by size:
3478 1279 474 317
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5548
SacI G_AGCT'C
Cuts at: 2205 2205
Size: 5548
SalI G'TCGA_C
Cuts at: 1936 2890 1936
Size: 954 4594
SapI GCTCTTCn'nnn_
Cuts at: 2024 2024
Size: 5548
SbfI CC_TGCA'GG
Cuts at: 2109 2583 2900 2109
Size: 474 317 4757
Fragments arranged by size:
4757 474 317
ScaI AGT'ACT
Cuts at: 3796 5508 3796
Size: 1712 3836
SfcI C'TryA_G
Cuts at: 826 1045 2105 2137 2579 2896 4031 4712 4903
Size: 219 1060 32 442 317 1135 681 191
Cuts at: 4903 826
Size: 1471
Fragments arranged by size:
1471 1135 1060 681 442 317 219 191
32
SfiI GGCCn_nnn'nGGCC
Cuts at: 2741 2741
Size: 5548
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5548
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5548
SmaI CCC'GGG
Cuts at: 1872 2875 1872
Size: 1003 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2048 2669 2774 3652 4553 4815
Size: 197 926 105 621 105 878 901 262
Cuts at: 4815 820
Size: 1553
Fragments arranged by size:
1553 926 901 878 621 262 197 105
105
SnaBI TAC'GTA
Cuts at: 493 3339 493
Size: 2846 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5548
SphI G_CATG'C
Cuts at: 1132 2351 2906 1132
Size: 1219 555 3774
Fragments arranged by size:
3774 1219 555
SspI AAT'ATT
Cuts at: 5 52 3472 5
Size: 47 3420 2081
Fragments arranged by size:
3420 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5548
StyI C'CwwG_G
Cuts at: 513 1065 2004 2987 513
Size: 552 939 983 3074
Fragments arranged by size:
3074 983 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3575 3592 3745 3930 4375 325
Size: 1255 1995 17 153 185 445 1498
Fragments arranged by size:
1995 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3794 5506 96
Size: 274 80 33 51 1443 1817 1712 138
Fragments arranged by size:
1817 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2343 2375 2657 2689 2705 2737 5508 5540 2343
Size: 32 282 32 16 32 2771 32 2351
Fragments arranged by size:
2771 2351 282 32 32 32 32 16
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5548
XbaI T'CTAG_A
Cuts at: 2884 2884
Size: 5548
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2173 2173
Size: 5548
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5548
XmaI C'CCGG_G
Cuts at: 1870 2873 1870
Size: 1003 4545
XmnI GAAnn'nnTTC
Cuts at: 3677 3677
Size: 5548
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3356 276
Size: 53 83 186 936 1822 2468
Fragments arranged by size:
2468 1822 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI
SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BstZ17I Bsu36I DraIII EcoRV FalI
FseI FspAI KasI NarI NruI NsiI PacI PmlI
PspOMI PspXI PsrI PvuII RsrII SacII SanDI SexAI
SfoI SrfI SwaI