Restriction map for
pFN21AE3109
(Circular) (Six-base) MAPSORT of: pFN21AE3109.seq Check: 1829 from: 1 to: 5539
pFN21AE3109 5539 bp
With 182 enzymes: SgfI *
December 28, 2011 15:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2398 2398
Size: 5539
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3349 278
Size: 53 83 186 936 1813 2468
Fragments arranged by size:
2468 1813 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2882 1937
Size: 945 4594
Acc65I G'GTAC_C
Cuts at: 2860 2860
Size: 5539
AclI AA'CG_TT
Cuts at: 1563 3666 4039 1563
Size: 2103 373 3063
Fragments arranged by size:
3063 2103 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2199 3602 4986 5254 1632
Size: 567 1403 1384 268 1917
Fragments arranged by size:
1917 1403 1384 567 268
AflII C'TTAA_G
Cuts at: 820 1017 2656 820
Size: 197 1639 3703
Fragments arranged by size:
3703 1639 197
AflIII A'CryG_T
Cuts at: 1203 4438 5415 1203
Size: 3235 977 1327
Fragments arranged by size:
3235 1327 977
AgeI A'CCGG_T
Cuts at: 4370 4370
Size: 5539
AhdI GACnn_n'nnGTC
Cuts at: 4268 4268
Size: 5539
AleI CACnn'nnGTG
Cuts at: 1166 2618 1166
Size: 1452 4087
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2636 1184
Size: 1452 4087
AlwNI CAG_nnn'CTG
Cuts at: 3282 4854 3282
Size: 1572 3967
ApaLI G'TGCA_C
Cuts at: 2621 3596 4752 2621
Size: 975 1156 3408
Fragments arranged by size:
3408 1156 975
ApoI r'AATT_y
Cuts at: 1493 2832 2848 3074 3226 5335 1493
Size: 1339 16 226 152 2109 1697
Fragments arranged by size:
2109 1697 1339 226 152 16
AseI AT'TA_AT
Cuts at: 160 1032 4093 160
Size: 872 3061 1606
Fragments arranged by size:
3061 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5539
AvaI C'yCGr_G
Cuts at: 1870 1943 2256 2864 1870
Size: 73 313 608 4545
Fragments arranged by size:
4545 608 313 73
BamHI G'GATC_C
Cuts at: 2869 3236 2869
Size: 367 5172
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2410 2860 4315 618
Size: 325 217 627 623 450 1455 1842
Fragments arranged by size:
1842 1455 627 623 450 325 217
BanII G_rGCy'C
Cuts at: 1391 2801 2858 1391
Size: 1410 57 4072
Fragments arranged by size:
4072 1410 57
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4568
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2330 2364 2551 2585 3730 3764 1903
Size: 34 393 34 187 34 1145 34 3678
Fragments arranged by size:
3678 1145 393 187 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2203 3431 4641 2203
Size: 1228 1210 3101
Fragments arranged by size:
3101 1228 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5539
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4150 136
Size: 107 122 71 631 605 2478 1525
Fragments arranged by size:
2478 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5534 5534
Size: 5539
BlpI GC'TnA_GC
Cuts at: 2573 2956 2573
Size: 383 5156
Bme1580I G_kGCm'C
Cuts at: 1790 2415 2625 3600 4756 1790
Size: 625 210 975 1156 2573
Fragments arranged by size:
2573 1156 975 625 210
BmgBI CAC'GTC
Cuts at: 1318 2077 1318
Size: 759 4780
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3270 4228 442
Size: 416 53 83 399 24 1853 958 1753
Fragments arranged by size:
1853 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5539
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4199 1239
Size: 2960 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2033 2654 3664 4529 4827 2033
Size: 621 1010 865 298 2745
Fragments arranged by size:
2745 1010 865 621 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4202 882
Size: 617 2703 2219
Fragments arranged by size:
2703 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3330 493
Size: 711 2126 2702
Fragments arranged by size:
2702 2126 711
BsaBI GATnn'nnATC
Cuts at: 5533 5533
Size: 5539
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3346 3728 4363 275
Size: 53 83 186 936 1813 382 635 1451
Fragments arranged by size:
1813 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3972 4370 4644 4791 5202 3972
Size: 398 274 147 411 4309
Fragments arranged by size:
4309 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5314
Fragments arranged by size:
5314 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5539
BseYI C'CCAG_C
Cuts at: 4742 5238 4742
Size: 496 5043
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2372 2596 2797 1163
Size: 573 636 224 201 3905
Fragments arranged by size:
3905 636 573 224 201
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2121 2561 2604 2989 3750 4778
Size: 275 186 120 440 43 385 761 1028
Cuts at: 4778 1540
Size: 2301
Fragments arranged by size:
2301 1028 761 440 385 275 186 120
43
BsiHKAI G_wGCw'C
Cuts at: 2625 3600 3685 4756 2625
Size: 975 85 1071 3408
Fragments arranged by size:
3408 1071 975 85
BsiWI C'GTAC_G
Cuts at: 2130 2130
Size: 5539
BsmI GAATG_Cn'
Cuts at: 2704 3050 3143 2704
Size: 346 93 5100
Fragments arranged by size:
5100 346 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2415 2625 2801 2858 3600 3685 4756
Size: 399 625 210 176 57 742 85 1071
Cuts at: 4756 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 625 399 210 176 85
57
BspEI T'CCGG_A
Cuts at: 5202 5202
Size: 5539
BspHI T'CATG_A
Cuts at: 3428 5158 3428
Size: 1730 3809
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2398 2894 844
Size: 1554 496 3489
Fragments arranged by size:
3489 1554 496
BsrBI CCG'CTC
Cuts at: 1394 1823 2247 2644 3426 1394
Size: 429 424 397 782 3507
Fragments arranged by size:
3507 782 429 424 397
BsrDI GCAATG_nn'
Cuts at: 65 1248 2289 2649 4034 4208 65
Size: 1183 1041 360 1385 174 1396
Fragments arranged by size:
1396 1385 1183 1041 360 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2547 2617 4183 4370 1700
Size: 110 737 70 1566 187 2869
Fragments arranged by size:
2869 1566 737 187 110 70
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5539
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5539
BssSI C'ACGA_G
Cuts at: 3599 4611 3599
Size: 1012 4527
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5539
BstBI TT'CG_AA
Cuts at: 4343 4343
Size: 5539
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5539
BstXI CCAn_nnnn'nTGG
Cuts at: 2176 2371 2176
Size: 195 5344
BstYI r'GATC_y
Cuts at: 1972 2091 2367 2869 3236 3621 3638 5090 5176
Size: 119 276 502 367 385 17 1452 86
Cuts at: 5176 5188 5534 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 502 385 367 346 276 119
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2415 513
Size: 552 1350 3637
Fragments arranged by size:
3637 1350 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4800
Fragments arranged by size:
4800 683 56
BtsI GCAGTG_nn'
Cuts at: 3055 3849 3869 3055
Size: 794 20 4725
Fragments arranged by size:
4725 794 20
ClaI AT'CG_AT
Cuts at: 1554 2323 2340 1554
Size: 769 17 4753
Fragments arranged by size:
4753 769 17
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5504
DraI TTT'AAA
Cuts at: 2843 2843
Size: 5539
DraIII CAC_nnn'GTG
Cuts at: 2270 2629 2270
Size: 359 5180
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4546 809
Size: 507 3230 1802
Fragments arranged by size:
3230 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2118 2134 2514
Size: 54 1425 50 275 18 288 16 380
Cuts at: 2514 2558 2685 2986 3875 5504 8
Size: 44 127 301 889 1629 43
Fragments arranged by size:
1629 1425 889 380 301 288 275 127
54 50 44 43 18 16
EagI C'GGCC_G
Cuts at: 1537 1812 2118 2558 2986 1537
Size: 275 306 440 428 4090
Fragments arranged by size:
4090 440 428 306 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3469 1353
Size: 2116 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2774 4126 4500 4646 387
Size: 1064 138 1185 1352 374 146 1280
Fragments arranged by size:
1352 1280 1185 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2199 3602 4199 4986 5254 1239
Size: 393 567 1403 597 787 268 1524
Fragments arranged by size:
1524 1403 787 597 567 393 268
EcoRI G'AATT_C
Cuts at: 2848 3226 2848
Size: 378 5161
FspI TGC'GCA
Cuts at: 4045 4045
Size: 5539
HaeII r_GCGC'y
Cuts at: 1678 2013 2446 4686 1678
Size: 335 433 2240 2531
Fragments arranged by size:
2531 2240 433 335
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4180 4212 4254 4286 1373
Size: 32 135 32 2608 32 42 32 2626
Fragments arranged by size:
2626 2608 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2883 3129 669
Size: 988 281 945 246 3079
Fragments arranged by size:
3079 988 945 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5539
HpaI GTT'AAC
Cuts at: 3129 3129
Size: 5539
KpnI G_GTAC'C
Cuts at: 2864 2864
Size: 5539
MfeI C'AATT_G
Cuts at: 3138 3138
Size: 5539
MluI A'CGCG_T
Cuts at: 5415 5415
Size: 5539
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4653 4837 1305
Size: 3348 184 2007
Fragments arranged by size:
3348 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2516 2687 10
Size: 54 1425 343 684 171 2862
Fragments arranged by size:
2862 1425 684 343 171 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2618 3497 3856 4015 518
Size: 648 1452 879 359 159 2042
Fragments arranged by size:
2042 1452 879 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2301 2826 2955 3632 4780 5025 1606
Size: 695 525 129 677 1148 245 2120
Fragments arranged by size:
2120 1148 695 677 525 245 129
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4987
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5539
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5539
NotI GC'GGCC_GC
Cuts at: 2986 2986
Size: 5539
NspI r_CATG'y
Cuts at: 1132 1229 2351 2897 4442 1132
Size: 97 1122 546 1545 2229
Fragments arranged by size:
2229 1545 1122 546 97
PasI CC'CwG_GG
Cuts at: 1348 2014 1348
Size: 666 4873
PciI A'CATG_T
Cuts at: 4438 4438
Size: 5539
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5539
PmeI GTTT'AAAC
Cuts at: 2843 2843
Size: 5539
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3608 3640 5121 5153 3608
Size: 32 1481 32 3994
Fragments arranged by size:
3994 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5539
PsiI TTA'TAA
Cuts at: 3109 3109
Size: 5539
PstI C_TGCA'G
Cuts at: 830 2109 2202 2891 830
Size: 1279 93 689 3478
Fragments arranged by size:
3478 1279 689 93
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5539
PvuII CAG'CTG
Cuts at: 2826 2826
Size: 5539
RsrII CG'GwC_CG
Cuts at: 2026 2026
Size: 5539
SalI G'TCGA_C
Cuts at: 1936 2881 1936
Size: 945 4594
SbfI CC_TGCA'GG
Cuts at: 2109 2891 2109
Size: 782 4757
ScaI AGT'ACT
Cuts at: 3787 5499 3787
Size: 1712 3827
SexAI A'CCwGG_T
Cuts at: 2625 2625
Size: 5539
SfcI C'TryA_G
Cuts at: 826 1045 2105 2198 2887 4022 4703 4894 826
Size: 219 1060 93 689 1135 681 191 1471
Fragments arranged by size:
1471 1135 1060 689 681 219 191 93
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5539
SgrAI Cr'CCGG_yG
Cuts at: 1700 2617 1700
Size: 917 4622
SmaI CCC'GGG
Cuts at: 1872 2258 2866 1872
Size: 386 608 4545
Fragments arranged by size:
4545 608 386
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2048 2656 2669 3643 4544 4806
Size: 197 926 105 608 13 974 901 262
Cuts at: 4806 820
Size: 1553
Fragments arranged by size:
1553 974 926 901 608 262 197 105
13
SnaBI TAC'GTA
Cuts at: 493 3330 493
Size: 2837 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5539
SphI G_CATG'C
Cuts at: 1132 2351 2897 1132
Size: 1219 546 3774
Fragments arranged by size:
3774 1219 546
SspI AAT'ATT
Cuts at: 5 52 3463 5
Size: 47 3411 2081
Fragments arranged by size:
3411 2081 47
StuI AGG'CCT
Cuts at: 1517 2432 1517
Size: 915 4624
StyI C'CwwG_G
Cuts at: 513 1065 2978 513
Size: 552 1913 3074
Fragments arranged by size:
3074 1913 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2762 3566 3583 3736 3921 4366 325
Size: 1255 1182 804 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1182 804 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3785 5497 96
Size: 274 80 33 51 1443 1808 1712 138
Fragments arranged by size:
1808 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2657 2689 2705 2737 5499 5531 2657
Size: 32 16 32 2762 32 2665
Fragments arranged by size:
2762 2665 32 32 32 16
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5539
XbaI T'CTAG_A
Cuts at: 2875 2875
Size: 5539
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2173 2371 2011
Size: 162 198 5179
Fragments arranged by size:
5179 198 162
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5539
XmaI C'CCGG_G
Cuts at: 1870 2256 2864 1870
Size: 386 608 4545
Fragments arranged by size:
4545 608 386
XmnI GAAnn'nnTTC
Cuts at: 3668 3668
Size: 5539
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3347 276
Size: 53 83 186 936 1813 2468
Fragments arranged by size:
2468 1813 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII
RsrII SalI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsmBI BstZ17I Bsu36I EcoICRI EcoNI
EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI SacI SacII SanDI SapI SfiI SfoI
SrfI SwaI