Restriction map for
pFN21AE3106
(Circular) (Six-base) MAPSORT of: pFN21AE3106.seq Check: 6848 from: 1 to: 5533
pFN21AE3106 5533 bp
With 182 enzymes: SgfI *
December 28, 2011 15:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2570 2570
Size: 5533
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3343 278
Size: 53 83 186 936 1807 2468
Fragments arranged by size:
2468 1807 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2876 1937
Size: 939 4594
Acc65I G'GTAC_C
Cuts at: 2854 2854
Size: 5533
AclI AA'CG_TT
Cuts at: 1563 3660 4033 1563
Size: 2097 373 3063
Fragments arranged by size:
3063 2097 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2840 3596 4980 5248 1632
Size: 1208 756 1384 268 1917
Fragments arranged by size:
1917 1384 1208 756 268
AfeI AGC'GCT
Cuts at: 2120 2120
Size: 5533
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5336
AflIII A'CryG_T
Cuts at: 1203 4432 5409 1203
Size: 3229 977 1327
Fragments arranged by size:
3229 1327 977
AgeI A'CCGG_T
Cuts at: 4364 4364
Size: 5533
AhdI GACnn_n'nnGTC
Cuts at: 4262 4262
Size: 5533
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5533
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5533
AlwNI CAG_nnn'CTG
Cuts at: 2818 3276 4848 2818
Size: 458 1572 3503
Fragments arranged by size:
3503 1572 458
ApaI G_GGCC'C
Cuts at: 2131 2192 2131
Size: 61 5472
ApaLI G'TGCA_C
Cuts at: 3590 4746 3590
Size: 1156 4377
ApoI r'AATT_y
Cuts at: 1493 2842 3068 3220 5329 1493
Size: 1349 226 152 2109 1697
Fragments arranged by size:
2109 1697 1349 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4087 160
Size: 872 3055 1606
Fragments arranged by size:
3055 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5533
AvaI C'yCGr_G
Cuts at: 1870 1943 2081 2185 2507 2858 1870
Size: 73 138 104 322 351 4545
Fragments arranged by size:
4545 351 322 138 104 73
BamHI G'GATC_C
Cuts at: 2863 3230 2863
Size: 367 5166
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2027 2076 2854 4309 618
Size: 325 217 627 240 49 778 1455 1842
Fragments arranged by size:
1842 1455 778 627 325 240 217 49
BanII G_rGCy'C
Cuts at: 1391 2131 2192 2224 2394 2448 2852 1391
Size: 740 61 32 170 54 404 4072
Fragments arranged by size:
4072 740 404 170 61 54 32
BbeI G_GCGC'C
Cuts at: 2031 2080 2031
Size: 49 5484
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2761 928
Size: 971 862 3700
Fragments arranged by size:
3700 971 862
BbvCI CC'TCA_GC
Cuts at: 2581 2581
Size: 5533
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3724 3758 1903
Size: 34 1787 34 3678
Fragments arranged by size:
3678 1787 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3425 4635 3425
Size: 1210 4323
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5533
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2036 2045 2273
Size: 107 122 71 631 605 364 9 228
Cuts at: 2273 4144 136
Size: 1871 1525
Fragments arranged by size:
1871 1525 631 605 364 228 122 107
71 9
BglII A'GATC_T
Cuts at: 5528 5528
Size: 5533
BlpI GC'TnA_GC
Cuts at: 2115 2950 2115
Size: 835 4698
Bme1580I G_kGCm'C
Cuts at: 1790 2057 2131 2192 2574 2631 3594 4750 1790
Size: 267 74 61 382 57 963 1156 2573
Fragments arranged by size:
2573 1156 963 382 267 74 61 57
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5533
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2319 3264 4222
Size: 416 53 83 399 24 902 945 958
Cuts at: 4222 442
Size: 1753
Fragments arranged by size:
1753 958 945 902 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5533
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2459 4193 1239
Size: 1220 1734 2579
Fragments arranged by size:
2579 1734 1220
Bpu10I CC'TnA_GC
Cuts at: 2531 2581 2531
Size: 50 5483
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3658 4523 4821 3658
Size: 865 298 4370
Fragments arranged by size:
4370 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2399 2534 4196 882
Size: 617 900 135 1662 2219
Fragments arranged by size:
2219 1662 900 617 135
BsaAI yAC'GTr
Cuts at: 493 1204 3324 493
Size: 711 2120 2702
Fragments arranged by size:
2702 2120 711
BsaBI GATnn'nnATC
Cuts at: 5527 5527
Size: 5533
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2028 2069 2077 2466
Size: 53 83 186 936 495 41 8 389
Cuts at: 2466 3340 3722 4357 275
Size: 874 382 635 1451
Fragments arranged by size:
1451 936 874 635 495 389 382 186
83 53 41 8
BsaWI w'CCGG_w
Cuts at: 3966 4364 4638 4785 5196 3966
Size: 398 274 147 411 4303
Fragments arranged by size:
4303 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5308
Fragments arranged by size:
5308 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2099 2232 2390 2552 2693 1186
Size: 913 133 158 162 141 4026
Fragments arranged by size:
4026 913 162 158 141 133
BseYI C'CCAG_C
Cuts at: 2336 2422 2575 4736 5232 2336
Size: 86 153 2161 496 2637
Fragments arranged by size:
2637 2161 496 153 86
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4960
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2789 2983 3744 4772 1540
Size: 275 186 788 194 761 1028 2301
Fragments arranged by size:
2301 1028 788 761 275 194 186
BsiHKAI G_wGCw'C
Cuts at: 3594 3679 4750 3594
Size: 85 1071 4377
Fragments arranged by size:
4377 1071 85
BsmI GAATG_Cn'
Cuts at: 3044 3137 3044
Size: 93 5440
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2057 2131 2192 2224 2394 2448 2574
Size: 399 267 74 61 32 170 54 126
Cuts at: 2574 2631 2852 3594 3679 4750 1391
Size: 57 221 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 267 221 170 126
85 74 61 57 54 32
BspEI T'CCGG_A
Cuts at: 5196 5196
Size: 5533
BspHI T'CATG_A
Cuts at: 3422 5152 3422
Size: 1730 3803
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2570 2787 2888 844
Size: 1726 217 101 3489
Fragments arranged by size:
3489 1726 217 101
BsrBI CCG'CTC
Cuts at: 1394 1823 3420 1394
Size: 429 1597 3507
Fragments arranged by size:
3507 1597 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4028 4202 65
Size: 1183 2780 174 1396
Fragments arranged by size:
2780 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2035 2549 4177 4364 1700
Size: 110 225 514 1628 187 2869
Fragments arranged by size:
2869 1628 514 225 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5533
BssHII G'CGCG_C
Cuts at: 1925 2237 2731 2733 1925
Size: 312 494 2 4725
Fragments arranged by size:
4725 494 312 2
BssSI C'ACGA_G
Cuts at: 3593 4605 3593
Size: 1012 4521
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5533
BstBI TT'CG_AA
Cuts at: 4337 4337
Size: 5533
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5533
BstYI r'GATC_y
Cuts at: 1972 2863 3230 3615 3632 5084 5170 5182 5528
Size: 891 367 385 17 1452 86 12 346
Cuts at: 5528 1972
Size: 1977
Fragments arranged by size:
1977 1452 891 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2100 2160 2209 2783 2789 513
Size: 552 1035 60 49 574 6 3257
Fragments arranged by size:
3257 1035 574 552 60 49 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2242 2701 487
Size: 683 56 1016 459 3319
Fragments arranged by size:
3319 1016 683 459 56
BtsI GCAGTG_nn'
Cuts at: 2280 3049 3843 3863 2280
Size: 769 794 20 3950
Fragments arranged by size:
3950 794 769 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5533
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5498
DraI TTT'AAA
Cuts at: 2837 2837
Size: 5533
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2765 4540 809
Size: 507 1449 1775 1802
Fragments arranged by size:
1802 1775 1449 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2037 2358 2786
Size: 54 1425 50 275 18 207 321 428
Cuts at: 2786 2980 3869 5498 8
Size: 194 889 1629 43
Fragments arranged by size:
1629 1425 889 428 321 275 207 194
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2786 2980 1537
Size: 275 974 194 4090
Fragments arranged by size:
4090 974 275 194
EarI CTCTTCn'nnn_
Cuts at: 1353 2645 2701 3463 1353
Size: 1292 56 762 3423
Fragments arranged by size:
3423 1292 762 56
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2243 4120 4494 4640 387
Size: 1064 138 654 1877 374 146 1280
Fragments arranged by size:
1877 1280 1064 654 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2459 2840 3596 4193 4980 5248 1239
Size: 393 827 381 756 597 787 268 1524
Fragments arranged by size:
1524 827 787 756 597 393 381 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2228 2534 2228
Size: 306 5227
EcoO109I rG'GnC_Cy
Cuts at: 2189 2479 2776 2189
Size: 290 297 4946
Fragments arranged by size:
4946 297 290
EcoRI G'AATT_C
Cuts at: 2842 3220 2842
Size: 378 5155
FseI GG_CCGG'CC
Cuts at: 2039 2039
Size: 5533
FspI TGC'GCA
Cuts at: 4039 4039
Size: 5533
HaeII r_GCGC'y
Cuts at: 1678 2031 2080 2122 4680 1678
Size: 353 49 42 2558 2531
Fragments arranged by size:
2558 2531 353 49 42
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2651 2683 4174 4206 4248
Size: 32 135 32 1079 32 1491 32 42
Cuts at: 4248 4280 1373
Size: 32 2626
Fragments arranged by size:
2626 1491 1079 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2877 3123 669
Size: 988 281 939 246 3079
Fragments arranged by size:
3079 988 939 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5533
HpaI GTT'AAC
Cuts at: 3123 3123
Size: 5533
KasI G'GCGC_C
Cuts at: 2027 2076 2027
Size: 49 5484
KpnI G_GTAC'C
Cuts at: 2858 2858
Size: 5533
MfeI C'AATT_G
Cuts at: 3132 3132
Size: 5533
MluI A'CGCG_T
Cuts at: 5409 5409
Size: 5533
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4647 4831 1305
Size: 3342 184 2007
Fragments arranged by size:
3342 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2360 10
Size: 54 1425 343 528 3183
Fragments arranged by size:
3183 1425 528 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3491 3850 4009 518
Size: 648 2325 359 159 2042
Fragments arranged by size:
2325 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2114 2162 2422 2791 2949 3626 4774 5019
Size: 508 48 260 369 158 677 1148 245
Cuts at: 5019 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 508 369 260 245 158
48
NaeI GCC'GGC
Cuts at: 2037 2551 2037
Size: 514 5019
NarI GG'CG_CC
Cuts at: 2028 2077 2028
Size: 49 5484
NcoI C'CATG_G
Cuts at: 513 1065 2209 513
Size: 552 1144 3837
Fragments arranged by size:
3837 1144 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5533
NgoMIV G'CCGG_C
Cuts at: 2035 2549 2035
Size: 514 5019
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5533
NotI GC'GGCC_GC
Cuts at: 2980 2980
Size: 5533
NspI r_CATG'y
Cuts at: 1132 1229 2731 2891 4436 1132
Size: 97 1502 160 1545 2229
Fragments arranged by size:
2229 1545 1502 160 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5533
PciI A'CATG_T
Cuts at: 4432 4432
Size: 5533
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5533
PfoI T'CCnGG_A
Cuts at: 2475 2475
Size: 5533
PmeI GTTT'AAAC
Cuts at: 2837 2837
Size: 5533
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3602 3634 5115 5147 3602
Size: 32 1481 32 3988
Fragments arranged by size:
3988 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2479 2776 2479
Size: 297 5236
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5533
PsiI TTA'TAA
Cuts at: 3103 3103
Size: 5533
PspOMI G'GGCC_C
Cuts at: 2127 2188 2127
Size: 61 5472
PstI C_TGCA'G
Cuts at: 830 2885 830
Size: 2055 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5533
PvuII CAG'CTG
Cuts at: 2422 2422
Size: 5533
SacII CC_GC'GG
Cuts at: 2163 2792 2163
Size: 629 4904
SalI G'TCGA_C
Cuts at: 1936 2875 1936
Size: 939 4594
SbfI CC_TGCA'GG
Cuts at: 2885 2885
Size: 5533
ScaI AGT'ACT
Cuts at: 3781 5493 3781
Size: 1712 3821
SfcI C'TryA_G
Cuts at: 826 1045 2881 4016 4697 4888 826
Size: 219 1836 1135 681 191 1471
Fragments arranged by size:
1836 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2029 2078 2029
Size: 49 5484
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5533
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5533
SmaI CCC'GGG
Cuts at: 1872 2187 2860 1872
Size: 315 673 4545
Fragments arranged by size:
4545 673 315
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3637 4538 4800 820
Size: 197 926 1694 901 262 1553
Fragments arranged by size:
1694 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3324 493
Size: 2831 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5533
SphI G_CATG'C
Cuts at: 1132 2731 2891 1132
Size: 1599 160 3774
Fragments arranged by size:
3774 1599 160
SrfI GCCC'GGGC
Cuts at: 2187 2187
Size: 5533
SspI AAT'ATT
Cuts at: 5 52 3457 5
Size: 47 3405 2081
Fragments arranged by size:
3405 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5533
StyI C'CwwG_G
Cuts at: 513 1065 2209 2972 513
Size: 552 1144 763 3074
Fragments arranged by size:
3074 1144 763 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2338 2350 2777 3560 3577 3730 3915
Size: 1255 758 12 427 783 17 153 185
Cuts at: 3915 4360 325
Size: 445 1498
Fragments arranged by size:
1498 1255 783 758 445 427 185 153
17 12
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2513 3779 5491
Size: 274 80 33 51 1443 536 1266 1712
Cuts at: 5491 96
Size: 138
Fragments arranged by size:
1712 1443 1266 536 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5493 5525 5493
Size: 32 5501
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5533
XbaI T'CTAG_A
Cuts at: 2869 2869
Size: 5533
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5533
XmaI C'CCGG_G
Cuts at: 1870 2185 2858 1870
Size: 315 673 4545
Fragments arranged by size:
4545 673 315
XmnI GAAnn'nnTTC
Cuts at: 3662 3662
Size: 5533
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3341 276
Size: 53 83 186 936 1807 2468
Fragments arranged by size:
2468 1807 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FseI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI
BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FspAI
NruI NsiI PacI PmlI PspXI PsrI RsrII SacI
SanDI SapI SexAI SfiI SwaI XcmI