Restriction map for
pFN21AE3103
(Circular) (Six-base) MAPSORT of: pFN21AE3103.seq Check: 547 from: 1 to: 5512
pFN21AE3103 5512 bp
With 182 enzymes: SgfI *
February 20, 2021 22:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2407 2407
Size: 5512
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3322 278
Size: 53 83 186 936 1786 2468
Fragments arranged by size:
2468 1786 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2855 1937
Size: 918 4594
Acc65I G'GTAC_C
Cuts at: 2833 2833
Size: 5512
AclI AA'CG_TT
Cuts at: 1563 3639 4012 1563
Size: 2076 373 3063
Fragments arranged by size:
3063 2076 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2005 2170 3575 4959 5227 1632
Size: 373 165 1405 1384 268 1917
Fragments arranged by size:
1917 1405 1384 373 268 165
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5315
AflIII A'CryG_T
Cuts at: 1203 4411 5388 1203
Size: 3208 977 1327
Fragments arranged by size:
3208 1327 977
AgeI A'CCGG_T
Cuts at: 4343 4343
Size: 5512
AhdI GACnn_n'nnGTC
Cuts at: 4241 4241
Size: 5512
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5512
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5512
AlwNI CAG_nnn'CTG
Cuts at: 3255 4827 3255
Size: 1572 3940
ApaI G_GGCC'C
Cuts at: 2128 2295 2336 2342 2564 2128
Size: 167 41 6 222 5076
Fragments arranged by size:
5076 222 167 41 6
ApaLI G'TGCA_C
Cuts at: 3569 4725 3569
Size: 1156 4356
ApoI r'AATT_y
Cuts at: 1493 2821 3047 3199 5308 1493
Size: 1328 226 152 2109 1697
Fragments arranged by size:
2109 1697 1328 226 152
AscI GG'CGCG_CC
Cuts at: 2046 2046
Size: 5512
AseI AT'TA_AT
Cuts at: 160 1032 4066 160
Size: 872 3034 1606
Fragments arranged by size:
3034 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5512
AvaI C'yCGr_G
Cuts at: 1870 1943 2335 2535 2579 2837 1870
Size: 73 392 200 44 258 4545
Fragments arranged by size:
4545 392 258 200 73 44
AvrII C'CTAG_G
Cuts at: 2128 2128
Size: 5512
BamHI G'GATC_C
Cuts at: 2842 3209 2842
Size: 367 5145
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2141 2833 4288 618
Size: 325 217 627 354 692 1455 1842
Fragments arranged by size:
1842 1455 692 627 354 325 217
BanII G_rGCy'C
Cuts at: 1391 2128 2247 2295 2336 2342 2373 2564 2831
Size: 737 119 48 41 6 31 191 267
Cuts at: 2831 1391
Size: 4072
Fragments arranged by size:
4072 737 267 191 119 48 41 31
6
BbeI G_GCGC'C
Cuts at: 2145 2145
Size: 5512
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4541
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2593 2627 3703 3737 1903
Size: 34 656 34 1076 34 3678
Fragments arranged by size:
3678 1076 656 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3404 4614 3404
Size: 1210 4302
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5512
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2031 2094 2331
Size: 107 122 71 631 605 359 63 237
Cuts at: 2331 4123 136
Size: 1792 1525
Fragments arranged by size:
1792 1525 631 605 359 237 122 107
71 63
BglII A'GATC_T
Cuts at: 5507 5507
Size: 5512
BlpI GC'TnA_GC
Cuts at: 2929 2929
Size: 5512
Bme1580I G_kGCm'C
Cuts at: 1790 2128 2220 2295 2336 2342 2564 3573 4729
Size: 338 92 75 41 6 222 1009 1156
Cuts at: 4729 1790
Size: 2573
Fragments arranged by size:
2573 1156 1009 338 222 92 75 41
6
BmgBI CAC'GTC
Cuts at: 1318 2481 1318
Size: 1163 4349
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2386 3243 4201
Size: 416 53 83 399 24 969 857 958
Cuts at: 4201 442
Size: 1753
Fragments arranged by size:
1753 969 958 857 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5512
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2246 2318 4172 1239
Size: 1007 72 1854 2579
Fragments arranged by size:
2579 1854 1007 72
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2345 3637 4502 4800 2345
Size: 1292 865 298 3057
Fragments arranged by size:
3057 1292 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4175 882
Size: 617 2676 2219
Fragments arranged by size:
2676 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3303 493
Size: 711 2099 2702
Fragments arranged by size:
2702 2099 711
BsaBI GATnn'nnATC
Cuts at: 5506 5506
Size: 5512
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2142 3319 3701 4336
Size: 53 83 186 936 609 1177 382 635
Cuts at: 4336 275
Size: 1451
Fragments arranged by size:
1451 1177 936 635 609 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3945 4343 4617 4764 5175 3945
Size: 398 274 147 411 4282
Fragments arranged by size:
4282 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2186 2216 1178
Size: 30 165 30 783 30 4474
Fragments arranged by size:
4474 783 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5512
BseYI C'CCAG_C
Cuts at: 2768 4715 5211 2768
Size: 1947 496 3069
Fragments arranged by size:
3069 1947 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2687 1163
Size: 573 951 3988
Fragments arranged by size:
3988 951 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2457 2790 2962 3723 4751 1540
Size: 275 186 456 333 172 761 1028 2301
Fragments arranged by size:
2301 1028 761 456 333 275 186 172
BsiHKAI G_wGCw'C
Cuts at: 2247 3573 3658 4729 2247
Size: 1326 85 1071 3030
Fragments arranged by size:
3030 1326 1071 85
BsmI GAATG_Cn'
Cuts at: 3023 3116 3023
Size: 93 5419
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2128 2220 2247 2295 2336 2342 2373
Size: 399 338 92 27 48 41 6 31
Cuts at: 2373 2564 2831 3573 3658 4729 1391
Size: 191 267 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 338 267 191 92
85 48 41 31 27 6
BspEI T'CCGG_A
Cuts at: 5175 5175
Size: 5512
BspHI T'CATG_A
Cuts at: 3401 5131 3401
Size: 1730 3782
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2407 2867 844
Size: 1563 460 3489
Fragments arranged by size:
3489 1563 460
BsrBI CCG'CTC
Cuts at: 1394 1823 2348 3399 1394
Size: 429 525 1051 3507
Fragments arranged by size:
3507 1051 525 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4007 4181 65
Size: 1183 2759 174 1396
Fragments arranged by size:
2759 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2549 2586 2738 2785 4156 4343 1700
Size: 110 739 37 152 47 1371 187 2869
Fragments arranged by size:
2869 1371 739 187 152 110 47 37
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5512
BssHII G'CGCG_C
Cuts at: 1925 2039 2046 2321 1925
Size: 114 7 275 5116
Fragments arranged by size:
5116 275 114 7
BssSI C'ACGA_G
Cuts at: 3572 4584 3572
Size: 1012 4500
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5512
BstBI TT'CG_AA
Cuts at: 4316 4316
Size: 5512
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5512
BstYI r'GATC_y
Cuts at: 1972 2842 3209 3594 3611 5063 5149 5161 5507
Size: 870 367 385 17 1452 86 12 346
Cuts at: 5507 1972
Size: 1977
Fragments arranged by size:
1977 1452 870 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2107 2205 2439 2569 513
Size: 552 1042 98 234 130 3456
Fragments arranged by size:
3456 1042 552 234 130 98
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2596 487
Size: 683 56 1370 3403
Fragments arranged by size:
3403 1370 683 56
BtsI GCAGTG_nn'
Cuts at: 3028 3822 3842 3028
Size: 794 20 4698
Fragments arranged by size:
4698 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5512
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5477
DraI TTT'AAA
Cuts at: 2816 2816
Size: 5512
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4519 809
Size: 507 3203 1802
Fragments arranged by size:
3203 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2208 2421 2454
Size: 54 1425 50 275 18 378 213 33
Cuts at: 2454 2736 2787 2959 3848 5477 8
Size: 282 51 172 889 1629 43
Fragments arranged by size:
1629 1425 889 378 282 275 213 172
54 51 50 43 33 18
EagI C'GGCC_G
Cuts at: 1537 1812 2454 2787 2959 1537
Size: 275 642 333 172 4090
Fragments arranged by size:
4090 642 333 275 172
EarI CTCTTCn'nnn_
Cuts at: 1353 2024 2252 2264 2537 3442 1353
Size: 671 228 12 273 905 3423
Fragments arranged by size:
3423 905 671 273 228 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4099 4473 4619 387
Size: 1064 138 2510 374 146 1280
Fragments arranged by size:
2510 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2245 2245
Size: 5512
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2005 2170 2246 2318 3575 4172 4959
Size: 393 373 165 76 72 1257 597 787
Cuts at: 4959 5227 1239
Size: 268 1524
Fragments arranged by size:
1524 1257 787 597 393 373 268 165
76 72
EcoO109I rG'GnC_Cy
Cuts at: 2124 2125 2291 2292 2339 2560 2731 2124
Size: 1 166 1 47 221 171 4905
Fragments arranged by size:
4905 221 171 166 47 1 1
EcoRI G'AATT_C
Cuts at: 2821 3199 2821
Size: 378 5134
FseI GG_CCGG'CC
Cuts at: 2742 2742
Size: 5512
FspI TGC'GCA
Cuts at: 2431 2621 4018 2431
Size: 190 1397 3925
Fragments arranged by size:
3925 1397 190
FspAI rTGC'GCAy
Cuts at: 2431 2431
Size: 5512
HaeII r_GCGC'y
Cuts at: 1678 2013 2145 2178 2193 2550 4659 1678
Size: 335 132 33 15 357 2109 2531
Fragments arranged by size:
2531 2109 357 335 132 33 15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4153 4185 4227 4259 1373
Size: 32 135 32 2581 32 42 32 2626
Fragments arranged by size:
2626 2581 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2856 3102 669
Size: 988 281 918 246 3079
Fragments arranged by size:
3079 988 918 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5512
HpaI GTT'AAC
Cuts at: 3102 3102
Size: 5512
KasI G'GCGC_C
Cuts at: 2141 2141
Size: 5512
KpnI G_GTAC'C
Cuts at: 2837 2837
Size: 5512
MfeI C'AATT_G
Cuts at: 3111 3111
Size: 5512
MluI A'CGCG_T
Cuts at: 5388 5388
Size: 5512
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4626 4810 1305
Size: 3321 184 2007
Fragments arranged by size:
3321 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3690
Fragments arranged by size:
3690 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2615 3470 3829 3988 518
Size: 648 1449 855 359 159 2042
Fragments arranged by size:
2042 1449 855 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2109 2571 2593 2764 2928 3605 4753 4998
Size: 503 462 22 171 164 677 1148 245
Cuts at: 4998 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 503 462 245 171 164
22
NaeI GCC'GGC
Cuts at: 2551 2740 2787 2551
Size: 189 47 5276
Fragments arranged by size:
5276 189 47
NarI GG'CG_CC
Cuts at: 2142 2142
Size: 5512
NcoI C'CATG_G
Cuts at: 513 1065 2205 513
Size: 552 1140 3820
Fragments arranged by size:
3820 1140 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5512
NgoMIV G'CCGG_C
Cuts at: 2549 2738 2785 2549
Size: 189 47 5276
Fragments arranged by size:
5276 189 47
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5512
NotI GC'GGCC_GC
Cuts at: 2454 2959 2454
Size: 505 5007
NspI r_CATG'y
Cuts at: 1132 1229 2870 4415 1132
Size: 97 1641 1545 2229
Fragments arranged by size:
2229 1641 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5512
PciI A'CATG_T
Cuts at: 4411 4411
Size: 5512
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2713 1108
Size: 1605 3907
PmeI GTTT'AAAC
Cuts at: 2816 2816
Size: 5512
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3581 3613 5094 5126 3581
Size: 32 1481 32 3967
Fragments arranged by size:
3967 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2731 2731
Size: 5512
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5512
PsiI TTA'TAA
Cuts at: 3082 3082
Size: 5512
PspOMI G'GGCC_C
Cuts at: 2124 2291 2332 2338 2560 2124
Size: 167 41 6 222 5076
Fragments arranged by size:
5076 222 167 41 6
PstI C_TGCA'G
Cuts at: 830 2864 830
Size: 2034 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5512
PvuII CAG'CTG
Cuts at: 2764 2764
Size: 5512
RsrII CG'GwC_CG
Cuts at: 2566 2566
Size: 5512
SacI G_AGCT'C
Cuts at: 2247 2247
Size: 5512
SacII CC_GC'GG
Cuts at: 2110 2572 2110
Size: 462 5050
SalI G'TCGA_C
Cuts at: 1936 2854 1936
Size: 918 4594
SapI GCTCTTCn'nnn_
Cuts at: 2024 2252 2537 2024
Size: 228 285 4999
Fragments arranged by size:
4999 285 228
SbfI CC_TGCA'GG
Cuts at: 2864 2864
Size: 5512
ScaI AGT'ACT
Cuts at: 3760 5472 3760
Size: 1712 3800
SfcI C'TryA_G
Cuts at: 826 1045 2860 3995 4676 4867 826
Size: 219 1815 1135 681 191 1471
Fragments arranged by size:
1815 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2143 2143
Size: 5512
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5512
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5512
SmaI CCC'GGG
Cuts at: 1872 2337 2537 2839 1872
Size: 465 200 302 4545
Fragments arranged by size:
4545 465 302 200
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2360 3616 4517 4779 820
Size: 197 926 417 1256 901 262 1553
Fragments arranged by size:
1553 1256 926 901 417 262 197
SnaBI TAC'GTA
Cuts at: 493 3303 493
Size: 2810 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5512
SphI G_CATG'C
Cuts at: 1132 2870 1132
Size: 1738 3774
SrfI GCCC'GGGC
Cuts at: 2337 2337
Size: 5512
SspI AAT'ATT
Cuts at: 5 52 3436 5
Size: 47 3384 2081
Fragments arranged by size:
3384 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5512
StyI C'CwwG_G
Cuts at: 513 1065 2128 2205 2328 2951 513
Size: 552 1063 77 123 623 3074
Fragments arranged by size:
3074 1063 623 552 123 77
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3539 3556 3709 3894 4339 325
Size: 1255 1959 17 153 185 445 1498
Fragments arranged by size:
1959 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3758 5470 96
Size: 274 80 33 51 1443 1781 1712 138
Fragments arranged by size:
1781 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5472 5504 5472
Size: 32 5480
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5512
XbaI T'CTAG_A
Cuts at: 2848 2848
Size: 5512
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5512
XmaI C'CCGG_G
Cuts at: 1870 2335 2535 2837 1870
Size: 465 200 302 4545
Fragments arranged by size:
4545 465 302 200
XmnI GAAnn'nnTTC
Cuts at: 3641 3641
Size: 5512
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3320 276
Size: 53 83 186 936 1786 2468
Fragments arranged by size:
2468 1786 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FseI
FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI BaeI BbvCI BfrBI BplI Bpu10I BsiWI
BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI
NruI NsiI PacI PfoI PmlI PspXI PsrI SanDI
SexAI SfiI SwaI XcmI