Restriction map for
pFN21AE3102
(Circular) (Six-base) MAPSORT of: pFN21AE3102.seq Check: 3800 from: 1 to: 5512
pFN21AE3102 5512 bp
With 182 enzymes: SgfI *
March 28, 2012 12:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3322 278
Size: 53 83 186 936 1786 2468
Fragments arranged by size:
2468 1786 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2855 1937
Size: 918 4594
Acc65I G'GTAC_C
Cuts at: 2833 2833
Size: 5512
AclI AA'CG_TT
Cuts at: 1563 3639 4012 1563
Size: 2076 373 3063
Fragments arranged by size:
3063 2076 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2700 3575 4959 5227 1632
Size: 1068 875 1384 268 1917
Fragments arranged by size:
1917 1384 1068 875 268
AflII C'TTAA_G
Cuts at: 820 1017 2327 820
Size: 197 1310 4005
Fragments arranged by size:
4005 1310 197
AflIII A'CryG_T
Cuts at: 1203 4411 5388 1203
Size: 3208 977 1327
Fragments arranged by size:
3208 1327 977
AgeI A'CCGG_T
Cuts at: 4343 4343
Size: 5512
AhdI GACnn_n'nnGTC
Cuts at: 4241 4241
Size: 5512
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5512
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5512
AlwNI CAG_nnn'CTG
Cuts at: 3255 4827 3255
Size: 1572 3940
ApaLI G'TGCA_C
Cuts at: 3569 4725 3569
Size: 1156 4356
ApoI r'AATT_y
Cuts at: 1493 2821 3047 3199 5308 1493
Size: 1328 226 152 2109 1697
Fragments arranged by size:
2109 1697 1328 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4066 160
Size: 872 3034 1606
Fragments arranged by size:
3034 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5512
AvaI C'yCGr_G
Cuts at: 1870 1943 2837 1870
Size: 73 894 4545
Fragments arranged by size:
4545 894 73
BamHI G'GATC_C
Cuts at: 2842 3209 2842
Size: 367 5145
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2833 4288 618
Size: 325 217 627 1046 1455 1842
Fragments arranged by size:
1842 1455 1046 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2047 2831 1391
Size: 656 784 4072
Fragments arranged by size:
4072 784 656
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4541
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3703 3737 1903
Size: 34 50 34 1682 34 3678
Fragments arranged by size:
3678 1682 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2609 3404 4614 2609
Size: 795 1210 3507
Fragments arranged by size:
3507 1210 795
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5512
BfrBI ATG'CAT
Cuts at: 2008 2008
Size: 5512
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2237 4123 136
Size: 107 122 71 631 605 565 1886 1525
Fragments arranged by size:
1886 1525 631 605 565 122 107 71
BglII A'GATC_T
Cuts at: 5507 5507
Size: 5512
BlpI GC'TnA_GC
Cuts at: 2929 2929
Size: 5512
Bme1580I G_kGCm'C
Cuts at: 1790 3573 4729 1790
Size: 1783 1156 2573
Fragments arranged by size:
2573 1783 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5512
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2502 3243 4201
Size: 416 53 83 399 24 1085 741 958
Cuts at: 4201 442
Size: 1753
Fragments arranged by size:
1753 1085 958 741 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5512
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4172 1239
Size: 2933 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3637 4502 4800 3637
Size: 865 298 4349
Fragments arranged by size:
4349 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2028 2188 4175 882
Size: 617 529 160 1987 2219
Fragments arranged by size:
2219 1987 617 529 160
BsaAI yAC'GTr
Cuts at: 493 1204 3303 493
Size: 711 2099 2702
Fragments arranged by size:
2702 2099 711
BsaBI GATnn'nnATC
Cuts at: 5506 5506
Size: 5512
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3319 3701 4336 275
Size: 53 83 186 936 1786 382 635 1451
Fragments arranged by size:
1786 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2224 3945 4343 4617 4764 5175 2224
Size: 1721 398 274 147 411 2561
Fragments arranged by size:
2561 1721 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5287
Fragments arranged by size:
5287 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2333 1186
Size: 1147 4365
BseYI C'CCAG_C
Cuts at: 4715 5211 4715
Size: 496 5016
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4939
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2199 2962 3723 4751 1540
Size: 275 186 198 763 761 1028 2301
Fragments arranged by size:
2301 1028 763 761 275 198 186
BsiHKAI G_wGCw'C
Cuts at: 3573 3658 4729 3573
Size: 85 1071 4356
Fragments arranged by size:
4356 1071 85
BsmI GAATG_Cn'
Cuts at: 3023 3116 3023
Size: 93 5419
BsmBI CGTCTCn'nnnn_
Cuts at: 2064 2272 2064
Size: 208 5304
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2047 2831 3573 3658 4729 1391
Size: 399 257 784 742 85 1071 2174
Fragments arranged by size:
2174 1071 784 742 399 257 85
BspEI T'CCGG_A
Cuts at: 2224 5175 2224
Size: 2951 2561
BspHI T'CATG_A
Cuts at: 3401 5131 3401
Size: 1730 3782
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2867 844
Size: 2023 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2140 3399 1394
Size: 429 317 1259 3507
Fragments arranged by size:
3507 1259 429 317
BsrDI GCAATG_nn'
Cuts at: 65 1248 2482 4007 4181 65
Size: 1183 1234 1525 174 1396
Fragments arranged by size:
1525 1396 1234 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4156 4343 1700
Size: 110 2346 187 2869
Fragments arranged by size:
2869 2346 187 110
BsrGI T'GTAC_A
Cuts at: 96 2408 96
Size: 2312 3200
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5512
BssSI C'ACGA_G
Cuts at: 3572 4584 3572
Size: 1012 4500
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5512
BstBI TT'CG_AA
Cuts at: 4316 4316
Size: 5512
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5512
BstYI r'GATC_y
Cuts at: 1972 2368 2842 3209 3594 3611 5063 5149 5161
Size: 396 474 367 385 17 1452 86 12
Cuts at: 5161 5507 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 474 396 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2039 513
Size: 552 974 3986
Fragments arranged by size:
3986 974 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4773
Fragments arranged by size:
4773 683 56
BtsI GCAGTG_nn'
Cuts at: 3028 3822 3842 3028
Size: 794 20 4698
Fragments arranged by size:
4698 794 20
ClaI AT'CG_AT
Cuts at: 1554 2602 1554
Size: 1048 4464
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5477
DraI TTT'AAA
Cuts at: 2016 2816 2016
Size: 800 4712
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4519 809
Size: 507 3203 1802
Fragments arranged by size:
3203 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2196 2204 2238
Size: 54 1425 50 275 18 366 8 34
Cuts at: 2238 2959 3848 5477 8
Size: 721 889 1629 43
Fragments arranged by size:
1629 1425 889 721 366 275 54 50
43 34 18 8
EagI C'GGCC_G
Cuts at: 1537 1812 2196 2959 1537
Size: 275 384 763 4090
Fragments arranged by size:
4090 763 384 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2062 3442 1353
Size: 709 1380 3423
Fragments arranged by size:
3423 1380 709
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2163 2169 2296 4099 4473 4619
Size: 1064 138 574 6 127 1803 374 146
Cuts at: 4619 387
Size: 1280
Fragments arranged by size:
1803 1280 1064 574 374 146 138 127
6
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2700 3575 4172 4959 5227 1239
Size: 393 1068 875 597 787 268 1524
Fragments arranged by size:
1524 1068 875 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2094 2094
Size: 5512
EcoO109I rG'GnC_Cy
Cuts at: 2099 2282 2338 2099
Size: 183 56 5273
Fragments arranged by size:
5273 183 56
EcoRI G'AATT_C
Cuts at: 2821 3199 2821
Size: 378 5134
FspI TGC'GCA
Cuts at: 4018 4018
Size: 5512
HaeII r_GCGC'y
Cuts at: 1678 4659 1678
Size: 2981 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2162 2194 4153 4185 4227
Size: 32 135 32 590 32 1959 32 42
Cuts at: 4227 4259 1373
Size: 32 2626
Fragments arranged by size:
2626 1959 590 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2404 2856 3102 669
Size: 988 281 466 452 246 3079
Fragments arranged by size:
3079 988 466 452 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5512
HpaI GTT'AAC
Cuts at: 2404 3102 2404
Size: 698 4814
KpnI G_GTAC'C
Cuts at: 2837 2837
Size: 5512
MfeI C'AATT_G
Cuts at: 3111 3111
Size: 5512
MluI A'CGCG_T
Cuts at: 5388 5388
Size: 5512
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2689 4626 4810 1305
Size: 1384 1937 184 2007
Fragments arranged by size:
2007 1937 1384 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3690
Fragments arranged by size:
3690 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3470 3829 3988 518
Size: 648 2304 359 159 2042
Fragments arranged by size:
2304 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2928 3605 4753 4998 1606
Size: 1322 677 1148 245 2120
Fragments arranged by size:
2120 1322 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4960
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5512
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5512
NotI GC'GGCC_GC
Cuts at: 2196 2959 2196
Size: 763 4749
NsiI A_TGCA'T
Cuts at: 2010 2010
Size: 5512
NspI r_CATG'y
Cuts at: 1132 1229 2870 4415 1132
Size: 97 1641 1545 2229
Fragments arranged by size:
2229 1641 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2286 1348
Size: 938 4574
PciI A'CATG_T
Cuts at: 4411 4411
Size: 5512
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5512
PmeI GTTT'AAAC
Cuts at: 2816 2816
Size: 5512
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3581 3613 5094 5126 3581
Size: 32 1481 32 3967
Fragments arranged by size:
3967 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2099 2282 2338 2099
Size: 183 56 5273
Fragments arranged by size:
5273 183 56
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5512
PsiI TTA'TAA
Cuts at: 3082 3082
Size: 5512
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2743 2775 2743
Size: 32 5480
PstI C_TGCA'G
Cuts at: 830 2864 830
Size: 2034 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5512
SalI G'TCGA_C
Cuts at: 1936 2854 1936
Size: 918 4594
SbfI CC_TGCA'GG
Cuts at: 2864 2864
Size: 5512
ScaI AGT'ACT
Cuts at: 3760 5472 3760
Size: 1712 3800
SfcI C'TryA_G
Cuts at: 826 1045 2277 2649 2860 3995 4676 4867 826
Size: 219 1232 372 211 1135 681 191 1471
Fragments arranged by size:
1471 1232 1135 681 372 219 211 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2237 2237
Size: 5512
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5512
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5512
SmaI CCC'GGG
Cuts at: 1872 2839 1872
Size: 967 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2327 3616 4517 4779 820
Size: 197 926 384 1289 901 262 1553
Fragments arranged by size:
1553 1289 926 901 384 262 197
SnaBI TAC'GTA
Cuts at: 493 3303 493
Size: 2810 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5512
SphI G_CATG'C
Cuts at: 1132 2870 1132
Size: 1738 3774
SspI AAT'ATT
Cuts at: 5 52 3436 5
Size: 47 3384 2081
Fragments arranged by size:
3384 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5512
StyI C'CwwG_G
Cuts at: 513 1065 2611 2951 513
Size: 552 1546 340 3074
Fragments arranged by size:
3074 1546 552 340
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2170 2461 3539 3556 3709 3894 4339
Size: 1255 590 291 1078 17 153 185 445
Cuts at: 4339 325
Size: 1498
Fragments arranged by size:
1498 1255 1078 590 445 291 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2408 3758 5470
Size: 274 80 33 51 1443 431 1350 1712
Cuts at: 5470 96
Size: 138
Fragments arranged by size:
1712 1443 1350 431 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5472 5504 5472
Size: 32 5480
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5512
XbaI T'CTAG_A
Cuts at: 2848 2848
Size: 5512
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5512
XmaI C'CCGG_G
Cuts at: 1870 2837 1870
Size: 967 4545
XmnI GAAnn'nnTTC
Cuts at: 3641 3641
Size: 5512
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3320 276
Size: 53 83 186 936 1786 2468
Fragments arranged by size:
2468 1786 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PsrI
PstI PvuI SalI SbfI ScaI SfcI SfiI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PvuII
RsrII SacI SacII SanDI SapI SexAI SfoI SrfI
SwaI XcmI