Restriction map for
pFN21AE3100
(Circular) (Six-base) MAPSORT of: pFN21AE3100.seq Check: 9361 from: 1 to: 10810
pFN21AE3100 10810 bp
With 182 enzymes: SgfI *
March 28, 2012 14:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3316 4444 6355 3316
Size: 1128 1911 7771
Fragments arranged by size:
7771 1911 1128
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2847 8620 278
Size: 53 83 186 936 1311 5773 2468
Fragments arranged by size:
5773 2468 1311 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2625 3887 6750 8153 1937
Size: 688 1262 2863 1403 4594
Fragments arranged by size:
4594 2863 1403 1262 688
Acc65I G'GTAC_C
Cuts at: 2508 3075 3722 8131 2508
Size: 567 647 4409 5187
Fragments arranged by size:
5187 4409 647 567
AclI AA'CG_TT
Cuts at: 1563 8937 9310 1563
Size: 7374 373 3063
Fragments arranged by size:
7374 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3854 4071 4190 4404 7577 7618 8873 10257
Size: 2222 217 119 214 3173 41 1255 1384
Cuts at: 10257 10525 1632
Size: 268 1917
Fragments arranged by size:
3173 2222 1917 1384 1255 268 217 214
119 41
AflII C'TTAA_G
Cuts at: 820 1017 4508 820
Size: 197 3491 7122
Fragments arranged by size:
7122 3491 197
AflIII A'CryG_T
Cuts at: 1203 2184 2808 3882 9709 10686 1203
Size: 981 624 1074 5827 977 1327
Fragments arranged by size:
5827 1327 1074 981 977 624
AgeI A'CCGG_T
Cuts at: 3473 6478 9641 3473
Size: 3005 3163 4642
Fragments arranged by size:
4642 3163 3005
AhdI GACnn_n'nnGTC
Cuts at: 4368 9539 4368
Size: 5171 5639
AleI CACnn'nnGTG
Cuts at: 1166 3731 3962 3992 4358 4409 5036 5138 5773
Size: 2565 231 30 366 51 627 102 635
Cuts at: 5773 5810 5839 6713 7286 7991 1166
Size: 37 29 874 573 705 3985
Fragments arranged by size:
3985 2565 874 705 635 627 573 366
231 102 51 37 30 29
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 5791 5857 6075 6239 1184
Size: 4607 66 218 164 5755
Fragments arranged by size:
5755 4607 218 164 66
AlwNI CAG_nnn'CTG
Cuts at: 2549 3322 3779 3923 4427 5816 6544 6562 6901
Size: 773 457 144 504 1389 728 18 339
Cuts at: 6901 7075 7102 7570 8553 10125 2549
Size: 174 27 468 983 1572 3234
Fragments arranged by size:
3234 1572 1389 983 773 728 504 468
457 339 174 144 27 18
ApaI G_GGCC'C
Cuts at: 2400 4149 4519 5560 6491 7632 7684 7869 2400
Size: 1749 370 1041 931 1141 52 185 5341
Fragments arranged by size:
5341 1749 1141 1041 931 370 185 52
ApaLI G'TGCA_C
Cuts at: 3908 8867 10023 3908
Size: 4959 1156 4695
Fragments arranged by size:
4959 4695 1156
ApoI r'AATT_y
Cuts at: 1493 8119 8345 8497 10606 1493
Size: 6626 226 152 2109 1697
Fragments arranged by size:
6626 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 9364 160
Size: 872 8332 1606
Fragments arranged by size:
8332 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10810
AvaI C'yCGr_G
Cuts at: 1870 1943 2417 2601 2967 3108 4519 5606 6038
Size: 73 474 184 366 141 1411 1087 432
Cuts at: 6038 6484 6830 8135 1870
Size: 446 346 1305 4545
Fragments arranged by size:
4545 1411 1305 1087 474 446 432 366
346 184 141 73
AvrII C'CTAG_G
Cuts at: 4651 4651
Size: 10810
BamHI G'GATC_C
Cuts at: 8140 8507 8140
Size: 367 10443
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2089 2508 2755 2824 3075
Size: 325 217 627 302 419 247 69 251
Cuts at: 3075 3403 3694 3722 4349 4532 4631 4667 4745
Size: 328 291 28 627 183 99 36 78
Cuts at: 4745 4897 5075 5261 5348 5387 5429 5697 5767
Size: 152 178 186 87 39 42 268 70
Cuts at: 5767 5903 5987 6101 6125 6190 6215 6287 6797
Size: 136 84 114 24 65 25 72 510
Cuts at: 6797 6947 7008 7463 7532 8030 8131 9586 618
Size: 150 61 455 69 498 101 1455 1842
Fragments arranged by size:
1842 1455 627 627 510 498 455 419
328 325 302 291 268 251 247 217
186 183 178 152 150 136 114 101
99 87 84 78 72 70 69 69
65 61 42 39 36 28 25 24
BanII G_rGCy'C
Cuts at: 1391 2136 2400 3287 4004 4149 4519 4836 5489
Size: 745 264 887 717 145 370 317 653
Cuts at: 5489 5560 5607 5760 6491 6633 6825 6960 7632
Size: 71 47 153 731 142 192 135 672
Cuts at: 7632 7684 7799 7818 7826 7869 8129 1391
Size: 52 115 19 8 43 260 4072
Fragments arranged by size:
4072 887 745 731 717 672 653 370
317 264 260 192 153 145 142 135
115 71 52 47 43 19 8
BbeI G_GCGC'C
Cuts at: 2759 4353 5391 5701 2759
Size: 1594 1038 310 7868
Fragments arranged by size:
7868 1594 1038 310
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3743 3842 928
Size: 971 1844 99 7896
Fragments arranged by size:
7896 1844 971 99
BbvCI CC'TCA_GC
Cuts at: 4183 4183
Size: 10810
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 7747 7781 9001 9035 1903
Size: 34 5810 34 1220 34 3678
Fragments arranged by size:
5810 3678 1220 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2902 2932 8702 9912 2902
Size: 30 5770 1210 3800
Fragments arranged by size:
5770 3800 1210 30
BclI T'GATC_A
Cuts at: 1548 4773 4890 1548
Size: 3225 117 7468
Fragments arranged by size:
7468 3225 117
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3527 3650 3659
Size: 107 122 71 631 605 1855 123 9
Cuts at: 3659 4232 5414 5465 5555 5831 6553 6911 7841
Size: 573 1182 51 90 276 722 358 930
Cuts at: 7841 9421 136
Size: 1580 1525
Fragments arranged by size:
1855 1580 1525 1182 930 722 631 605
573 358 276 123 122 107 90 71
51 9
BglII A'GATC_T
Cuts at: 10805 10805
Size: 10810
BlpI GC'TnA_GC
Cuts at: 5663 8227 5663
Size: 2564 8246
Bme1580I G_kGCm'C
Cuts at: 1790 2400 2424 3429 3601 3912 4149 4519 4554
Size: 610 24 1005 172 311 237 370 35
Cuts at: 4554 4748 5264 5351 5560 5846 5990 6029 6130
Size: 194 516 87 209 286 144 39 101
Cuts at: 6130 6290 6491 6950 7011 7466 7535 7632 7684
Size: 160 201 459 61 455 69 97 52
Cuts at: 7684 7869 8871 10027 1790
Size: 185 1002 1156 2573
Fragments arranged by size:
2573 1156 1005 1002 610 516 459 455
370 311 286 237 209 201 194 185
172 160 144 101 97 87 69 61
52 39 35 24
BmgBI CAC'GTC
Cuts at: 1318 2727 3885 7659 1318
Size: 1409 1158 3774 4469
Fragments arranged by size:
4469 3774 1409 1158
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2733 4272 5472
Size: 416 53 83 399 24 1316 1539 1200
Cuts at: 5472 5491 8541 9499 442
Size: 19 3050 958 1753
Fragments arranged by size:
3050 1753 1539 1316 1200 958 416 399
83 53 24 19
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 10810
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7287 7759 7287
Size: 472 10338
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2327 2571 2966 3164 3173 3179 4449 5492
Size: 1088 244 395 198 9 6 1270 1043
Cuts at: 5492 5771 5849 6394 6500 6905 7879 8049 9470
Size: 279 78 545 106 405 974 170 1421
Cuts at: 9470 1239
Size: 2579
Fragments arranged by size:
2579 1421 1270 1088 1043 974 545 405
395 279 244 198 170 106 78 9
6
Bpu10I CC'TnA_GC
Cuts at: 4183 6627 4183
Size: 2444 8366
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3429 4747 7802 8935 9800 10098 3429
Size: 1318 3055 1133 865 298 4141
Fragments arranged by size:
4141 3055 1318 1133 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2918 5054 5240 5327 5882 5966 6266
Size: 617 1419 2136 186 87 555 84 300
Cuts at: 6266 9473 882
Size: 3207 2219
Fragments arranged by size:
3207 2219 2136 1419 617 555 300 186
87 84
BsaAI yAC'GTr
Cuts at: 493 1204 2185 2809 8601 493
Size: 711 981 624 5792 2702
Fragments arranged by size:
5792 2702 981 711 624
BsaBI GATnn'nnATC
Cuts at: 5447 10804 5447
Size: 5357 5453
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2384 2756 2844 4106
Size: 53 83 186 936 851 372 88 1262
Cuts at: 4106 4350 5388 5698 6141 8617 8999 9634 275
Size: 244 1038 310 443 2476 382 635 1451
Fragments arranged by size:
2476 1451 1262 1038 936 851 635 443
382 372 310 244 186 88 83 53
BsaWI w'CCGG_w
Cuts at: 2086 3250 3473 5445 6478 7711 9243 9641 9915
Size: 1164 223 1972 1033 1233 1532 398 274
Cuts at: 9915 10062 10473 2086
Size: 147 411 2423
Fragments arranged by size:
2423 1972 1532 1233 1164 1033 411 398
274 223 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2414 2444 2861 2891 3007
Size: 30 165 30 1011 30 417 30 116
Cuts at: 3007 3037 3122 3152 3481 3511 1178
Size: 30 85 30 329 30 8477
Fragments arranged by size:
8477 1011 417 329 165 116 85 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3784 6848 1186
Size: 2598 3064 5148
Fragments arranged by size:
5148 3064 2598
BseYI C'CCAG_C
Cuts at: 2218 2689 2820 3103 3153 3292 3530 3715 3772
Size: 471 131 283 50 139 238 185 57
Cuts at: 3772 3936 4869 4917 5127 5750 5801 6194 7003
Size: 164 933 48 210 623 51 393 809
Cuts at: 7003 7119 7180 7226 7296 7318 7406 7805 7836
Size: 116 61 46 70 22 88 399 31
Cuts at: 7836 7868 10013 10509 2218
Size: 32 2145 496 2519
Fragments arranged by size:
2519 2145 933 809 623 496 471 399
393 283 238 210 185 164 139 131
116 88 70 61 57 51 50 48
46 32 31 22
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2165 2271 3335 5036 5162 5442 5651
Size: 573 429 106 1064 1701 126 280 209
Cuts at: 5651 7109 7907 1163
Size: 1458 798 4066
Fragments arranged by size:
4066 1701 1458 1064 798 573 429 280
209 126 106
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2016 3663 4803 5571 6639 7020
Size: 275 186 15 1647 1140 768 1068 381
Cuts at: 7020 8260 9021 10049 1540
Size: 1240 761 1028 2301
Fragments arranged by size:
2301 1647 1240 1140 1068 1028 768 761
381 275 186 15
BsiHKAI G_wGCw'C
Cuts at: 2136 2968 3912 4030 4435 5045 5567 5957 6257
Size: 832 944 118 405 610 522 390 300
Cuts at: 6257 6380 6633 6921 7723 7799 7818 7826 8871
Size: 123 253 288 802 76 19 8 1045
Cuts at: 8871 8956 10027 2136
Size: 85 1071 2919
Fragments arranged by size:
2919 1071 1045 944 832 802 610 522
405 390 300 288 253 123 118 85
76 19 8
BsmI GAATG_Cn'
Cuts at: 5935 8321 8414 5935
Size: 2386 93 8331
Fragments arranged by size:
8331 2386 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4276 4594 4678 5249 5336 5975 6275 4276
Size: 318 84 571 87 639 300 8811
Fragments arranged by size:
8811 639 571 318 300 87 84
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2136 2400 2424 2968 3287 3429 3601
Size: 399 346 264 24 544 319 142 172
Cuts at: 3601 3912 4004 4030 4149 4435 4519 4554 4748
Size: 311 92 26 119 286 84 35 194
Cuts at: 4748 4836 5045 5264 5351 5489 5560 5567 5607
Size: 88 209 219 87 138 71 7 40
Cuts at: 5607 5760 5846 5957 5990 6029 6130 6257 6290
Size: 153 86 111 33 39 101 127 33
Cuts at: 6290 6380 6491 6633 6825 6921 6950 6960 7011
Size: 90 111 142 192 96 29 10 51
Cuts at: 7011 7466 7535 7632 7684 7723 7799 7818 7826
Size: 455 69 97 52 39 76 19 8
Cuts at: 7826 7869 8129 8871 8956 10027 1391
Size: 43 260 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 544 455 399 346 319
311 286 264 260 219 209 194 192
172 153 142 142 138 127 119 111
111 101 97 96 92 90 88 87
86 85 84 76 71 69 52 51
43 40 39 39 35 33 33 29
26 24 19 10 8 7
BspEI T'CCGG_A
Cuts at: 5445 7711 10473 5445
Size: 2266 2762 5782
Fragments arranged by size:
5782 2762 2266
BspHI T'CATG_A
Cuts at: 8699 10429 8699
Size: 1730 9080
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3316 3526 4444 6355 7401 8165 844
Size: 2472 210 918 1911 1046 764 3489
Fragments arranged by size:
3489 2472 1911 1046 918 764 210
BsrBI CCG'CTC
Cuts at: 1394 1823 8697 1394
Size: 429 6874 3507
Fragments arranged by size:
6874 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2332 4749 7969 9305 9479 65
Size: 1183 1084 2417 3220 1336 174 1396
Fragments arranged by size:
3220 2417 1396 1336 1183 1084 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2352 3473 5570 6478 6689 6950 7214
Size: 110 542 1121 2097 908 211 261 264
Cuts at: 7214 7300 9454 9641 1700
Size: 86 2154 187 2869
Fragments arranged by size:
2869 2154 2097 1121 908 542 264 261
211 187 110 86
BsrGI T'GTAC_A
Cuts at: 96 2187 3006 96
Size: 2091 819 7900
Fragments arranged by size:
7900 2091 819
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 10810
BssSI C'ACGA_G
Cuts at: 2861 5066 5252 5339 5894 5978 6278 8870 9882
Size: 2205 186 87 555 84 300 2592 1012
Cuts at: 9882 2861
Size: 3789
Fragments arranged by size:
3789 2592 2205 1012 555 300 186 87
84
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2448 6562 7102 7570 794
Size: 1654 4114 540 468 4034
Fragments arranged by size:
4114 4034 1654 540 468
BstBI TT'CG_AA
Cuts at: 5231 9614 5231
Size: 4383 6427
BstEII G'GTnAC_C
Cuts at: 1184 3505 3952 3973 4177 1184
Size: 2321 447 21 204 7817
Fragments arranged by size:
7817 2321 447 204 21
BstXI CCAn_nnnn'nTGG
Cuts at: 2890 2935 3142 3964 3991 4255 5072 5207 6715
Size: 45 207 822 27 264 817 135 1508
Cuts at: 6715 6811 7234 7474 2890
Size: 96 423 240 6226
Fragments arranged by size:
6226 1508 822 817 423 264 240 207
135 96 45 27
BstYI r'GATC_y
Cuts at: 1972 3678 6646 8140 8507 8892 8909 10361 10447
Size: 1706 2968 1494 367 385 17 1452 86
Cuts at: 10447 10459 10805 1972
Size: 12 346 1977
Fragments arranged by size:
2968 1977 1706 1494 1452 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3480 3480
Size: 10810
BtgI C'CryG_G
Cuts at: 513 1065 2004 2093 2104 2118 2619 2913 3216
Size: 552 939 89 11 14 501 294 303
Cuts at: 3216 3699 3741 4110 4353 4477 4554 6162 6551
Size: 483 42 369 243 124 77 1608 389
Cuts at: 6551 6642 6882 6960 513
Size: 91 240 78 4363
Fragments arranged by size:
4363 1608 939 552 501 483 389 369
303 294 243 240 124 91 89 78
77 42 14 11
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4975 7936 487
Size: 683 56 3749 2961 3361
Fragments arranged by size:
3749 3361 2961 683 56
BtsI GCAGTG_nn'
Cuts at: 2977 3168 4030 4449 4782 5358 5834 6849 8326
Size: 191 862 419 333 576 476 1015 1477
Cuts at: 8326 9120 9140 2977
Size: 794 20 4647
Fragments arranged by size:
4647 1477 1015 862 794 576 476 419
333 191 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 10810
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4603 4638 4666 4701 5083 5118 7600
Size: 35 4039 35 28 35 382 35 2482
Cuts at: 7600 7635 7979 8014 529
Size: 35 344 35 3325
Fragments arranged by size:
4039 3325 2482 382 344 35 35 35
35 35 35 28
DraI TTT'AAA
Cuts at: 8114 8114
Size: 10810
DraIII CAC_nnn'GTG
Cuts at: 3596 4904 5057 5885 3596
Size: 1308 153 828 8521
Fragments arranged by size:
8521 1308 828 153
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6166 6430 9817 809
Size: 507 4850 264 3387 1802
Fragments arranged by size:
4850 3387 1802 507 264
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2013 2107 2121
Size: 54 1425 50 275 18 183 94 14
Cuts at: 2121 2469 2540 2853 2974 3642 3651 3660 4329
Size: 348 71 313 121 668 9 9 669
Cuts at: 4329 4474 4722 4800 5088 5298 5457 5547 5568
Size: 145 248 78 288 210 159 90 21
Cuts at: 5568 5850 6000 6300 6636 6705 6780 6903 7017
Size: 282 150 300 336 69 75 123 114
Cuts at: 7017 7212 7254 7311 7356 7621 7785 7983 8257
Size: 195 42 57 45 265 164 198 274
Cuts at: 8257 9146 10775 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 669 668 348 336 313
300 288 282 275 274 265 248 210
198 195 183 164 159 150 145 123
121 114 94 90 78 75 71 69
57 54 50 45 43 42 21 18
14 9 9
EagI C'GGCC_G
Cuts at: 1537 1812 2013 3660 4800 5568 6636 7017 8257
Size: 275 201 1647 1140 768 1068 381 1240
Cuts at: 8257 1537
Size: 4090
Fragments arranged by size:
4090 1647 1240 1140 1068 768 381 275
201
EarI CTCTTCn'nnn_
Cuts at: 1353 2360 2766 3992 8740 1353
Size: 1007 406 1226 4748 3423
Fragments arranged by size:
4748 3423 1226 1007 406
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3862 4582 6586 7013 9397 9771
Size: 1064 138 2273 720 2004 427 2384 374
Cuts at: 9771 9917 387
Size: 146 1280
Fragments arranged by size:
2384 2273 2004 1280 1064 720 427 374
146 138
EcoICRI GAG'CTC
Cuts at: 2134 6631 7797 7816 7824 2134
Size: 4497 1166 19 8 5120
Fragments arranged by size:
5120 4497 1166 19 8
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2327 2571 2966 3164 3173 3179 3854
Size: 393 695 244 395 198 9 6 675
Cuts at: 3854 4071 4190 4404 4449 5492 5771 5849 6394
Size: 217 119 214 45 1043 279 78 545
Cuts at: 6394 6500 6905 7577 7618 7879 8049 8873 9470
Size: 106 405 672 41 261 170 824 597
Cuts at: 9470 10257 10525 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1043 824 787 695 675 672 597
545 405 395 393 279 268 261 244
217 214 198 170 119 106 78 45
41 9 6
EcoNI CCTnn'n_nnAGG
Cuts at: 3350 3350
Size: 10810
EcoO109I rG'GnC_Cy
Cuts at: 2397 2485 2587 2695 3067 4115 4145 4146 4515
Size: 88 102 108 372 1048 30 1 369
Cuts at: 4515 4516 5556 5557 5683 6488 6530 6604 6898
Size: 1 1040 1 126 805 42 74 294
Cuts at: 6898 7150 7628 7629 7680 7681 2397
Size: 252 478 1 51 1 5526
Fragments arranged by size:
5526 1048 1040 805 478 372 369 294
252 126 108 102 88 74 51 42
30 1 1 1 1 1
EcoRI G'AATT_C
Cuts at: 8119 8497 8119
Size: 378 10432
FseI GG_CCGG'CC
Cuts at: 5574 5574
Size: 10810
FspI TGC'GCA
Cuts at: 2983 9316 2983
Size: 6333 4477
HaeII r_GCGC'y
Cuts at: 1678 2759 4353 5391 5471 5701 5943 7241 9957
Size: 1081 1594 1038 80 230 242 1298 2716
Cuts at: 9957 1678
Size: 2531
Fragments arranged by size:
2716 2531 1594 1298 1081 1038 242 230
80
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3463 3495 4024 4056 4354
Size: 32 135 32 1891 32 529 32 298
Cuts at: 4354 4386 6593 6625 6743 6775 9451 9483 9525
Size: 32 2207 32 118 32 2676 32 42
Cuts at: 9525 9557 1373
Size: 32 2626
Fragments arranged by size:
2676 2626 2207 1891 529 298 135 118
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 8154 8400 669
Size: 988 281 6216 246 3079
Fragments arranged by size:
6216 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 10810
HpaI GTT'AAC
Cuts at: 8400 8400
Size: 10810
KasI G'GCGC_C
Cuts at: 2755 4349 5387 5697 2755
Size: 1594 1038 310 7868
Fragments arranged by size:
7868 1594 1038 310
KpnI G_GTAC'C
Cuts at: 2512 3079 3726 8135 2512
Size: 567 647 4409 5187
Fragments arranged by size:
5187 4409 647 567
MfeI C'AATT_G
Cuts at: 7195 8409 7195
Size: 1214 9596
MluI A'CGCG_T
Cuts at: 10686 10686
Size: 10810
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5070 5316 5898 5982 6282 6342 7302 7929
Size: 3765 246 582 84 300 60 960 627
Cuts at: 7929 9924 10108 1305
Size: 1995 184 2007
Fragments arranged by size:
3765 2007 1995 960 627 582 300 246
184 84 60
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2123 2471 2855 2976 4476
Size: 54 1425 343 291 348 384 121 1500
Cuts at: 4476 4724 5459 5549 6707 6905 7313 7358 7787
Size: 248 735 90 1158 198 408 45 429
Cuts at: 7787 10
Size: 3033
Fragments arranged by size:
3033 1500 1425 1158 735 429 408 384
348 343 291 248 198 121 90 54
45
MslI CAynn'nnrTG
Cuts at: 518 1166 2141 2933 3422 3731 3746 3827 3905
Size: 648 975 792 489 309 15 81 78
Cuts at: 3905 3962 3992 4322 4358 4409 4793 4853 5036
Size: 57 30 330 36 51 384 60 183
Cuts at: 5036 5138 5534 5540 5706 5773 5810 5839 6107
Size: 102 396 6 166 67 37 29 268
Cuts at: 6107 6713 6809 7286 7853 7991 8768 9127 9286
Size: 606 96 477 567 138 777 359 159
Cuts at: 9286 518
Size: 2042
Fragments arranged by size:
2042 975 792 777 648 606 567 489
477 396 384 359 330 309 268 183
166 159 138 102 96 81 78 67
60 57 51 37 36 30 29 15
6
MspA1I CmG'CkG
Cuts at: 1606 2039 2058 2748 3322 3656 3665 3719 4157
Size: 433 19 690 574 334 9 54 438
Cuts at: 4157 4823 5171 5426 5723 6544 6866 7102 7555
Size: 666 348 255 297 821 322 236 453
Cuts at: 7555 7840 8226 8903 10051 10296 1606
Size: 285 386 677 1148 245 2120
Fragments arranged by size:
2120 1148 821 690 677 666 574 453
438 433 386 348 334 322 297 285
255 245 236 54 19 9
NaeI GCC'GGC
Cuts at: 5572 6691 7216 5572
Size: 1119 525 9166
Fragments arranged by size:
9166 1119 525
NarI GG'CG_CC
Cuts at: 2756 4350 5388 5698 2756
Size: 1594 1038 310 7868
Fragments arranged by size:
7868 1594 1038 310
NcoI C'CATG_G
Cuts at: 513 1065 2004 2913 3741 4554 6960 513
Size: 552 939 909 828 813 2406 4363
Fragments arranged by size:
4363 2406 939 909 828 813 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 10810
NgoMIV G'CCGG_C
Cuts at: 5570 6689 7214 5570
Size: 1119 525 9166
Fragments arranged by size:
9166 1119 525
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 10810
NotI GC'GGCC_GC
Cuts at: 3660 8257 3660
Size: 4597 6213
NspI r_CATG'y
Cuts at: 1132 1229 3832 8168 9713 1132
Size: 97 2603 4336 1545 2229
Fragments arranged by size:
4336 2603 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2243 3601 5482 6382 7375 7676 1348
Size: 895 1358 1881 900 993 301 4482
Fragments arranged by size:
4482 1881 1358 993 900 895 301
PciI A'CATG_T
Cuts at: 9709 9709
Size: 10810
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2208 2549 2919 5582 6901 1108
Size: 1100 341 370 2663 1319 5017
Fragments arranged by size:
5017 2663 1319 1100 370 341
PfoI T'CCnGG_A
Cuts at: 2529 2529
Size: 10810
PmeI GTTT'AAAC
Cuts at: 8114 8114
Size: 10810
PmlI CAC'GTG
Cuts at: 2185 2185
Size: 10810
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 8879 8911 10392 10424 8879
Size: 32 1481 32 9265
Fragments arranged by size:
9265 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2485 2587 2695 3067 4115 5683 6530 6604 2485
Size: 102 108 372 1048 1568 847 74 6691
Fragments arranged by size:
6691 1568 1048 847 372 108 102 74
PshAI GACnn'nnGTC
Cuts at: 1613 5864 1613
Size: 4251 6559
PsiI TTA'TAA
Cuts at: 8380 8380
Size: 10810
PspOMI G'GGCC_C
Cuts at: 2396 4145 4515 5556 6487 7628 7680 7865 2396
Size: 1749 370 1041 931 1141 52 185 5341
Fragments arranged by size:
5341 1749 1141 1041 931 370 185 52
PstI C_TGCA'G
Cuts at: 830 2052 2687 3844 6567 6859 7107 7332 7339
Size: 1222 635 1157 2723 292 248 225 7
Cuts at: 7339 7575 8007 8162 830
Size: 236 432 155 3478
Fragments arranged by size:
3478 2723 1222 1157 635 432 292 248
236 225 155 7
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 10810
PvuII CAG'CTG
Cuts at: 5171 5426 6544 6866 7102 7555 7840 5171
Size: 255 1118 322 236 453 285 8141
Fragments arranged by size:
8141 1118 453 322 285 255 236
SacI G_AGCT'C
Cuts at: 2136 6633 7799 7818 7826 2136
Size: 4497 1166 19 8 5120
Fragments arranged by size:
5120 4497 1166 19 8
SalI G'TCGA_C
Cuts at: 1936 8152 1936
Size: 6216 4594
SapI GCTCTTCn'nnn_
Cuts at: 3992 3992
Size: 10810
SbfI CC_TGCA'GG
Cuts at: 8162 8162
Size: 10810
ScaI AGT'ACT
Cuts at: 7772 9058 10770 7772
Size: 1286 1712 7812
Fragments arranged by size:
7812 1712 1286
SexAI A'CCwGG_T
Cuts at: 3051 3051
Size: 10810
SfcI C'TryA_G
Cuts at: 826 1045 2048 2683 3840 4962 5367 6563 6855
Size: 219 1003 635 1157 1122 405 1196 292
Cuts at: 6855 7103 7328 7335 7571 8003 8158 9293 9974
Size: 248 225 7 236 432 155 1135 681
Cuts at: 9974 10165 826
Size: 191 1471
Fragments arranged by size:
1471 1196 1157 1135 1122 1003 681 635
432 405 292 248 236 225 219 191
155 7
SfiI GGCCn_nnn'nGGCC
Cuts at: 3650 3659 5555 7841 3650
Size: 9 1896 2286 6619
Fragments arranged by size:
6619 2286 1896 9
SfoI GGC'GCC
Cuts at: 2757 4351 5389 5699 2757
Size: 1594 1038 310 7868
Fragments arranged by size:
7868 1594 1038 310
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10810
SgrAI Cr'CCGG_yG
Cuts at: 1700 6950 1700
Size: 5250 5560
SmaI CCC'GGG
Cuts at: 1872 2603 4521 5608 6486 8137 1872
Size: 731 1918 1087 878 1651 4545
Fragments arranged by size:
4545 1918 1651 1087 878 731
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3444 4508 4762 7817 8914 9815
Size: 197 926 1501 1064 254 3055 1097 901
Cuts at: 9815 10077 820
Size: 262 1553
Fragments arranged by size:
3055 1553 1501 1097 1064 926 901 262
254 197
SnaBI TAC'GTA
Cuts at: 493 8601 493
Size: 8108 2702
SpeI A'CTAG_T
Cuts at: 152 4709 152
Size: 4557 6253
SphI G_CATG'C
Cuts at: 1132 3832 8168 1132
Size: 2700 4336 3774
Fragments arranged by size:
4336 3774 2700
SspI AAT'ATT
Cuts at: 5 52 8734 5
Size: 47 8682 2081
Fragments arranged by size:
8682 2081 47
StuI AGG'CCT
Cuts at: 1517 3041 1517
Size: 1524 9286
StyI C'CwwG_G
Cuts at: 513 1065 2004 2504 2913 3741 4407 4554 4651
Size: 552 939 500 409 828 666 147 97
Cuts at: 4651 4740 5034 5113 5581 6960 8249 513
Size: 89 294 79 468 1379 1289 3074
Fragments arranged by size:
3074 1379 1289 939 828 666 552 500
468 409 294 147 97 89 79
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2235 2812 3259 3686 3712 4355 4892
Size: 1255 655 577 447 427 26 643 537
Cuts at: 4892 5150 5735 6008 6103 6113 6208 6395 6748
Size: 258 585 273 95 10 95 187 353
Cuts at: 6748 7364 7367 7420 8837 8854 9007 9192 9637
Size: 616 3 53 1417 17 153 185 445
Cuts at: 9637 325
Size: 1498
Fragments arranged by size:
1498 1417 1255 655 643 616 585 577
537 447 445 427 353 273 258 187
185 153 95 95 53 26 17 10
3
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2187 3006 3112
Size: 274 80 33 51 1443 210 819 106
Cuts at: 3112 7770 7857 9056 10768 96
Size: 4658 87 1199 1712 138
Fragments arranged by size:
4658 1712 1443 1199 819 274 210 138
106 87 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2414 2446 2861 2893 3007 3039 3433 3465 7421
Size: 32 415 32 114 32 394 32 3956
Cuts at: 7421 7453 7473 7505 10770 10802 2414
Size: 32 20 32 3265 32 2422
Fragments arranged by size:
3956 3265 2422 415 394 114 32 32
32 32 32 32 32 20
Tth111I GACn'n_nGTC
Cuts at: 1316 6469 1316
Size: 5153 5657
XbaI T'CTAG_A
Cuts at: 8146 8146
Size: 10810
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3784 4738 5101 5293 5467 5542 6041 6583 6808
Size: 954 363 192 174 75 499 542 225
Cuts at: 6808 7150 7264 7285 3784
Size: 342 114 21 7309
Fragments arranged by size:
7309 954 542 499 363 342 225 192
174 114 75 21
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 10810
XmaI C'CCGG_G
Cuts at: 1870 2601 4519 5606 6484 8135 1870
Size: 731 1918 1087 878 1651 4545
Fragments arranged by size:
4545 1918 1651 1087 878 731
XmnI GAAnn'nnTTC
Cuts at: 8939 8939
Size: 10810
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2845 8618 276
Size: 53 83 186 936 1311 5773 2468
Fragments arranged by size:
5773 2468 1311 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI BaeI BfrBI BsiWI BstZ17I EcoRV
FalI FspAI NruI NsiI PacI PspXI PsrI RsrII
SacII SanDI SrfI SwaI