Restriction map for
pFN21AE3098
(Circular) (Six-base) MAPSORT of: pFN21AE3098.seq Check: 1751 from: 1 to: 6010
pFN21AE3098 6010 bp
With 182 enzymes: SgfI *
September 4, 2015 17:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3153 3187 3153
Size: 34 5976
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3820 278
Size: 53 83 186 936 2284 2468
Fragments arranged by size:
2468 2284 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3291 3353 1937
Size: 1354 62 4594
Fragments arranged by size:
4594 1354 62
Acc65I G'GTAC_C
Cuts at: 3331 3331
Size: 6010
AclI AA'CG_TT
Cuts at: 1563 4137 4510 1563
Size: 2574 373 3063
Fragments arranged by size:
3063 2574 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2357 3158 4073 5457 5725 1632
Size: 725 801 915 1384 268 1917
Fragments arranged by size:
1917 1384 915 801 725 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5813
AflIII A'CryG_T
Cuts at: 1203 2727 4909 5886 1203
Size: 1524 2182 977 1327
Fragments arranged by size:
2182 1524 1327 977
AgeI A'CCGG_T
Cuts at: 4841 4841
Size: 6010
AhdI GACnn_n'nnGTC
Cuts at: 4739 4739
Size: 6010
AleI CACnn'nnGTG
Cuts at: 1166 3005 1166
Size: 1839 4171
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6010
AlwNI CAG_nnn'CTG
Cuts at: 2630 3753 5325 2630
Size: 1123 1572 3315
Fragments arranged by size:
3315 1572 1123
ApaI G_GGCC'C
Cuts at: 2064 2874 2936 3280 2064
Size: 810 62 344 4794
Fragments arranged by size:
4794 810 344 62
ApaLI G'TGCA_C
Cuts at: 2750 4067 5223 2750
Size: 1317 1156 3537
Fragments arranged by size:
3537 1317 1156
ApoI r'AATT_y
Cuts at: 1493 2825 3319 3545 3697 5806 1493
Size: 1332 494 226 152 2109 1697
Fragments arranged by size:
2109 1697 1332 494 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4564 160
Size: 872 3532 1606
Fragments arranged by size:
3532 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6010
AvaI C'yCGr_G
Cuts at: 1870 1943 2039 2049 2063 2831 2935 3149 3335
Size: 73 96 10 14 768 104 214 186
Cuts at: 3335 1870
Size: 4545
Fragments arranged by size:
4545 768 214 186 104 96 73 14
10
BamHI G'GATC_C
Cuts at: 3340 3707 3340
Size: 367 5643
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3331 4786 618
Size: 325 217 627 1544 1455 1842
Fragments arranged by size:
1842 1544 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2064 2114 2607 2859 2874 2936 3280 3329
Size: 673 50 493 252 15 62 344 49
Cuts at: 3329 1391
Size: 4072
Fragments arranged by size:
4072 673 493 344 252 62 50 49
15
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2258 2458 3241 928
Size: 971 359 200 783 3697
Fragments arranged by size:
3697 971 783 359 200
BbvCI CC'TCA_GC
Cuts at: 2623 2623
Size: 6010
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4201 4235 1903
Size: 34 2264 34 3678
Fragments arranged by size:
3678 2264 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3098 3902 5112 3098
Size: 804 1210 3996
Fragments arranged by size:
3996 1210 804
BclI T'GATC_A
Cuts at: 1548 3117 1548
Size: 1569 4441
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2647 4621 136
Size: 107 122 71 631 605 975 1974 1525
Fragments arranged by size:
1974 1525 975 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2814 6005 2814
Size: 3191 2819
BlpI GC'TnA_GC
Cuts at: 2591 3427 2591
Size: 836 5174
Bme1580I G_kGCm'C
Cuts at: 1790 2064 2754 2874 2936 3280 4071 5227 1790
Size: 274 690 120 62 344 791 1156 2573
Fragments arranged by size:
2573 1156 791 690 344 274 120 62
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6010
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3741 4699 442
Size: 416 53 83 399 24 2324 958 1753
Fragments arranged by size:
2324 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6010
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2509 2708 2813 3083 3197 4670 1239
Size: 1270 199 105 270 114 1473 2579
Fragments arranged by size:
2579 1473 1270 270 199 114 105
Bpu10I CC'TnA_GC
Cuts at: 2623 2623
Size: 6010
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4135 5000 5298 4135
Size: 865 298 4847
Fragments arranged by size:
4847 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2359 2712 3218 4673 882
Size: 617 860 353 506 1455 2219
Fragments arranged by size:
2219 1455 860 617 506 353
BsaAI yAC'GTr
Cuts at: 493 1204 2730 3801 493
Size: 711 1526 1071 2702
Fragments arranged by size:
2702 1526 1071 711
BsaBI GATnn'nnATC
Cuts at: 6004 6004
Size: 6010
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2009 2384 3817 4199
Size: 53 83 186 936 476 375 1433 382
Cuts at: 4199 4834 275
Size: 635 1451
Fragments arranged by size:
1451 1433 936 635 476 382 375 186
83 53
BsaWI w'CCGG_w
Cuts at: 4443 4841 5115 5262 5673 4443
Size: 398 274 147 411 4780
Fragments arranged by size:
4780 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2090 2120 2339 2369 3035
Size: 30 165 30 687 30 219 30 666
Cuts at: 3035 3065 1178
Size: 30 4123
Fragments arranged by size:
4123 687 666 219 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2659 1186
Size: 1473 4537
BseYI C'CCAG_C
Cuts at: 3279 5213 5709 3279
Size: 1934 496 3580
Fragments arranged by size:
3580 1934 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2732 1163
Size: 573 996 4441
Fragments arranged by size:
4441 996 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2167 2209 3460 4221 5249 1540
Size: 275 186 166 42 1251 761 1028 2301
Fragments arranged by size:
2301 1251 1028 761 275 186 166 42
BsiHKAI G_wGCw'C
Cuts at: 2754 2859 3014 3273 4071 4156 5227 2754
Size: 105 155 259 798 85 1071 3537
Fragments arranged by size:
3537 1071 798 259 155 105 85
BsmI GAATG_Cn'
Cuts at: 3521 3614 3521
Size: 93 5917
BsmBI CGTCTCn'nnnn_
Cuts at: 2413 3001 2413
Size: 588 5422
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2064 2114 2607 2754 2859 2874 2936
Size: 399 274 50 493 147 105 15 62
Cuts at: 2936 3014 3273 3280 3329 4071 4156 5227 1391
Size: 78 259 7 49 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 493 399 274 259 147
105 85 78 62 50 49 15 7
BspEI T'CCGG_A
Cuts at: 5673 5673
Size: 6010
BspHI T'CATG_A
Cuts at: 3899 5629 3899
Size: 1730 4280
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3153 3187 3365 844
Size: 2309 34 178 3489
Fragments arranged by size:
3489 2309 178 34
BsrBI CCG'CTC
Cuts at: 1394 1823 2617 3096 3897 1394
Size: 429 794 479 801 3507
Fragments arranged by size:
3507 801 794 479 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2661 3237 4505 4679 65
Size: 1183 1413 576 1268 174 1396
Fragments arranged by size:
1413 1396 1268 1183 576 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2166 4654 4841 1700
Size: 110 356 2488 187 2869
Fragments arranged by size:
2869 2488 356 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6010
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6010
BssSI C'ACGA_G
Cuts at: 3205 4070 5082 3205
Size: 865 1012 4133
Fragments arranged by size:
4133 1012 865
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6010
BstBI TT'CG_AA
Cuts at: 3252 4814 3252
Size: 1562 4448
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6010
BstXI CCAn_nnnn'nTGG
Cuts at: 2344 2344
Size: 6010
BstYI r'GATC_y
Cuts at: 1972 2814 3340 3707 4092 4109 5561 5647 5659
Size: 842 526 367 385 17 1452 86 12
Cuts at: 5659 6005 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 842 526 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4519
Fragments arranged by size:
4519 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5271
Fragments arranged by size:
5271 683 56
BtsI GCAGTG_nn'
Cuts at: 3526 4320 4340 3526
Size: 794 20 5196
Fragments arranged by size:
5196 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6010
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5975
DraI TTT'AAA
Cuts at: 3314 3314
Size: 6010
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5017 809
Size: 507 3701 1802
Fragments arranged by size:
3701 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2145 2164 2206
Size: 54 1425 50 275 18 315 19 42
Cuts at: 2206 2758 3457 4346 5975 8
Size: 552 699 889 1629 43
Fragments arranged by size:
1629 1425 889 699 552 315 275 54
50 43 42 19 18
EagI C'GGCC_G
Cuts at: 1537 1812 2164 2206 3457 1537
Size: 275 352 42 1251 4090
Fragments arranged by size:
4090 1251 352 275 42
EarI CTCTTCn'nnn_
Cuts at: 1353 3940 1353
Size: 2587 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2185 4597 4971 5117 387
Size: 1064 138 596 2412 374 146 1280
Fragments arranged by size:
2412 1280 1064 596 374 146 138
EcoICRI GAG'CTC
Cuts at: 2857 2857
Size: 6010
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2357 2509 2708 2813 3083 3158 3197
Size: 393 725 152 199 105 270 75 39
Cuts at: 3197 4073 4670 5457 5725 1239
Size: 876 597 787 268 1524
Fragments arranged by size:
1524 876 787 725 597 393 270 268
199 152 105 75 39
EcoNI CCTnn'n_nnAGG
Cuts at: 2075 2348 2075
Size: 273 5737
EcoO109I rG'GnC_Cy
Cuts at: 2068 2140 2870 2871 2932 3276 2068
Size: 72 730 1 61 344 4802
Fragments arranged by size:
4802 730 344 72 61 1
EcoRI G'AATT_C
Cuts at: 3319 3697 3319
Size: 378 5632
FspI TGC'GCA
Cuts at: 4516 4516
Size: 6010
HaeII r_GCGC'y
Cuts at: 1678 3076 5157 1678
Size: 1398 2081 2531
Fragments arranged by size:
2531 2081 1398
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2339 2371 4651 4683 4725
Size: 32 135 32 767 32 2280 32 42
Cuts at: 4725 4757 1373
Size: 32 2626
Fragments arranged by size:
2626 2280 767 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3354 3600 669
Size: 988 281 1416 246 3079
Fragments arranged by size:
3079 1416 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6010
HpaI GTT'AAC
Cuts at: 3600 3600
Size: 6010
KpnI G_GTAC'C
Cuts at: 3335 3335
Size: 6010
MfeI C'AATT_G
Cuts at: 3609 3609
Size: 6010
MluI A'CGCG_T
Cuts at: 5886 5886
Size: 6010
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2037 5124 5308 1305
Size: 732 3087 184 2007
Fragments arranged by size:
3087 2007 732 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2147 10
Size: 54 1425 343 315 3873
Fragments arranged by size:
3873 1425 343 315 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2708 3005 3968 4327 4486 518
Size: 648 1542 297 963 359 159 2042
Fragments arranged by size:
2042 1542 963 648 359 297 159
MspA1I CmG'CkG
Cuts at: 1606 2019 2627 3426 4103 5251 5496 1606
Size: 413 608 799 677 1148 245 2120
Fragments arranged by size:
2120 1148 799 677 608 413 245
NaeI GCC'GGC
Cuts at: 2168 2168
Size: 6010
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4519
Fragments arranged by size:
4519 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6010
NgoMIV G'CCGG_C
Cuts at: 2166 2166
Size: 6010
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6010
NotI GC'GGCC_GC
Cuts at: 3457 3457
Size: 6010
NspI r_CATG'y
Cuts at: 1132 1229 3368 4913 1132
Size: 97 2139 1545 2229
Fragments arranged by size:
2229 2139 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6010
PciI A'CATG_T
Cuts at: 4909 4909
Size: 6010
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2019 1108
Size: 911 5099
PfoI T'CCnGG_A
Cuts at: 2133 2685 2925 2133
Size: 552 240 5218
Fragments arranged by size:
5218 552 240
PmeI GTTT'AAAC
Cuts at: 3314 3314
Size: 6010
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4079 4111 5592 5624 4079
Size: 32 1481 32 4465
Fragments arranged by size:
4465 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6010
PsiI TTA'TAA
Cuts at: 3580 3580
Size: 6010
PspOMI G'GGCC_C
Cuts at: 2060 2870 2932 3276 2060
Size: 810 62 344 4794
Fragments arranged by size:
4794 810 344 62
PstI C_TGCA'G
Cuts at: 830 2427 3198 3362 830
Size: 1597 771 164 3478
Fragments arranged by size:
3478 1597 771 164
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6010
PvuII CAG'CTG
Cuts at: 2019 2627 2019
Size: 608 5402
SacI G_AGCT'C
Cuts at: 2859 2859
Size: 6010
SalI G'TCGA_C
Cuts at: 1936 3352 1936
Size: 1416 4594
SbfI CC_TGCA'GG
Cuts at: 3198 3362 3198
Size: 164 5846
ScaI AGT'ACT
Cuts at: 4258 5970 4258
Size: 1712 4298
SexAI A'CCwGG_T
Cuts at: 2319 2349 2319
Size: 30 5980
SfcI C'TryA_G
Cuts at: 826 1045 2423 3194 3358 4493 5174 5365 826
Size: 219 1378 771 164 1135 681 191 1471
Fragments arranged by size:
1471 1378 1135 771 681 219 191 164
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6010
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6010
SmaI CCC'GGG
Cuts at: 1872 2041 2051 2937 3337 1872
Size: 169 10 886 400 4545
Fragments arranged by size:
4545 886 400 169 10
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4114 5015 5277 820
Size: 197 926 2171 901 262 1553
Fragments arranged by size:
2171 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3801 493
Size: 3308 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6010
SphI G_CATG'C
Cuts at: 1132 3368 1132
Size: 2236 3774
SspI AAT'ATT
Cuts at: 5 52 3934 5
Size: 47 3882 2081
Fragments arranged by size:
3882 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6010
StyI C'CwwG_G
Cuts at: 513 1065 2004 2358 3003 3449 513
Size: 552 939 354 645 446 3074
Fragments arranged by size:
3074 939 645 552 446 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4037 4054 4207 4392 4837 325
Size: 1255 2457 17 153 185 445 1498
Fragments arranged by size:
2457 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2964 3111 3156
Size: 274 80 33 51 1443 987 147 45
Cuts at: 3156 4256 5968 96
Size: 1100 1712 138
Fragments arranged by size:
1712 1443 1100 987 274 147 138 80
51 45 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2090 2122 5970 6002 2090
Size: 32 3848 32 2098
Fragments arranged by size:
3848 2098 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2351 1316
Size: 1035 4975
XbaI T'CTAG_A
Cuts at: 3346 3346
Size: 6010
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3196 3196
Size: 6010
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6010
XmaI C'CCGG_G
Cuts at: 1870 2039 2049 2935 3335 1870
Size: 169 10 886 400 4545
Fragments arranged by size:
4545 886 400 169 10
XmnI GAAnn'nnTTC
Cuts at: 2432 4139 2432
Size: 1707 4303
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3818 276
Size: 53 83 186 936 2284 2468
Fragments arranged by size:
2468 2284 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI
KasI NarI NruI NsiI PacI PmlI PpuMI PspXI
PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI
SwaI