Restriction map for
pFN21AE3074
(Circular) (Six-base) MAPSORT of: pFN21AE3074.seq Check: 984 from: 1 to: 5407
pFN21AE3074 5407 bp
With 182 enzymes: SgfI *
March 28, 2012 12:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3217 278
Size: 53 83 186 936 1681 2468
Fragments arranged by size:
2468 1681 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2750 1937
Size: 813 4594
Acc65I G'GTAC_C
Cuts at: 2728 2728
Size: 5407
AclI AA'CG_TT
Cuts at: 1563 3534 3907 1563
Size: 1971 373 3063
Fragments arranged by size:
3063 1971 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3470 4854 5122 1632
Size: 1838 1384 268 1917
Fragments arranged by size:
1917 1838 1384 268
AfeI AGC'GCT
Cuts at: 2392 2392
Size: 5407
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5210
AflIII A'CryG_T
Cuts at: 1203 4306 5283 1203
Size: 3103 977 1327
Fragments arranged by size:
3103 1327 977
AgeI A'CCGG_T
Cuts at: 4238 4238
Size: 5407
AhdI GACnn_n'nnGTC
Cuts at: 4136 4136
Size: 5407
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5407
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2230 1184
Size: 1046 4361
AlwNI CAG_nnn'CTG
Cuts at: 2620 3150 4722 2620
Size: 530 1572 3305
Fragments arranged by size:
3305 1572 530
ApaLI G'TGCA_C
Cuts at: 3464 4620 3464
Size: 1156 4251
ApoI r'AATT_y
Cuts at: 1493 2716 2942 3094 5203 1493
Size: 1223 226 152 2109 1697
Fragments arranged by size:
2109 1697 1223 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3961 160
Size: 872 2929 1606
Fragments arranged by size:
2929 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5407
AvaI C'yCGr_G
Cuts at: 1870 1943 2047 2065 2732 1870
Size: 73 104 18 667 4545
Fragments arranged by size:
4545 667 104 73 18
BamHI G'GATC_C
Cuts at: 2340 2737 3104 2340
Size: 397 367 4643
Fragments arranged by size:
4643 397 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2069 2728 4183 618
Size: 325 217 627 282 659 1455 1842
Fragments arranged by size:
1842 1455 659 627 325 282 217
BanII G_rGCy'C
Cuts at: 1391 2575 2726 1391
Size: 1184 151 4072
Fragments arranged by size:
4072 1184 151
BbeI G_GCGC'C
Cuts at: 2073 2073
Size: 5407
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4436
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3598 3632 1903
Size: 34 50 34 1577 34 3678
Fragments arranged by size:
3678 1577 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3299 4509 3299
Size: 1210 4197
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5407
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4018 136
Size: 107 122 71 631 605 2346 1525
Fragments arranged by size:
2346 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5402 5402
Size: 5407
BlpI GC'TnA_GC
Cuts at: 2824 2824
Size: 5407
Bme1580I G_kGCm'C
Cuts at: 1790 3468 4624 1790
Size: 1678 1156 2573
Fragments arranged by size:
2573 1678 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5407
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3138 4096 442
Size: 416 53 83 399 24 1721 958 1753
Fragments arranged by size:
1753 1721 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5407
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4067 1239
Size: 2828 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2159 3532 4397 4695 2159
Size: 1373 865 298 2871
Fragments arranged by size:
2871 1373 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2216 4070 882
Size: 617 717 1854 2219
Fragments arranged by size:
2219 1854 717 617
BsaAI yAC'GTr
Cuts at: 493 1204 3198 493
Size: 711 1994 2702
Fragments arranged by size:
2702 1994 711
BsaBI GATnn'nnATC
Cuts at: 5401 5401
Size: 5407
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2070 2301 3214 3596
Size: 53 83 186 936 537 231 913 382
Cuts at: 3596 4231 275
Size: 635 1451
Fragments arranged by size:
1451 936 913 635 537 382 231 186
83 53
BsaWI w'CCGG_w
Cuts at: 3840 4238 4512 4659 5070 3840
Size: 398 274 147 411 4177
Fragments arranged by size:
4177 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5182
Fragments arranged by size:
5182 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2033 1186
Size: 847 4560
BseYI C'CCAG_C
Cuts at: 4610 5106 4610
Size: 496 4911
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2315 2540 1163
Size: 573 579 225 4030
Fragments arranged by size:
4030 579 573 225
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2170 2857 3618 4646 1540
Size: 275 186 169 687 761 1028 2301
Fragments arranged by size:
2301 1028 761 687 275 186 169
BsiHKAI G_wGCw'C
Cuts at: 2054 2555 3468 3553 4624 2054
Size: 501 913 85 1071 2837
Fragments arranged by size:
2837 1071 913 501 85
BsmI GAATG_Cn'
Cuts at: 2207 2918 3011 2207
Size: 711 93 4603
Fragments arranged by size:
4603 711 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2054 2555 2575 2726 3468 3553 4624
Size: 399 264 501 20 151 742 85 1071
Cuts at: 4624 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 501 399 264 151 85
20
BspEI T'CCGG_A
Cuts at: 5070 5070
Size: 5407
BspHI T'CATG_A
Cuts at: 3296 5026 3296
Size: 1730 3677
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2160 2337 2685 2762 844
Size: 1316 177 348 77 3489
Fragments arranged by size:
3489 1316 348 177 77
BsrBI CCG'CTC
Cuts at: 1394 1823 2013 3294 1394
Size: 429 190 1281 3507
Fragments arranged by size:
3507 1281 429 190
BsrDI GCAATG_nn'
Cuts at: 65 1248 3902 4076 65
Size: 1183 2654 174 1396
Fragments arranged by size:
2654 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2101 2126 4051 4238 1700
Size: 110 291 25 1925 187 2869
Fragments arranged by size:
2869 1925 291 187 110 25
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5407
BssHII G'CGCG_C
Cuts at: 1925 2160 1925
Size: 235 5172
BssSI C'ACGA_G
Cuts at: 3467 4479 3467
Size: 1012 4395
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2456 794
Size: 1662 3745
BstBI TT'CG_AA
Cuts at: 4211 4211
Size: 5407
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5407
BstXI CCAn_nnnn'nTGG
Cuts at: 2593 2593
Size: 5407
BstYI r'GATC_y
Cuts at: 1972 2340 2610 2737 3104 3489 3506 4958 5044
Size: 368 270 127 367 385 17 1452 86
Cuts at: 5044 5056 5402 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 385 368 367 346 270 127
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2116 513
Size: 552 1051 3804
Fragments arranged by size:
3804 1051 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4668
Fragments arranged by size:
4668 683 56
BtsI GCAGTG_nn'
Cuts at: 2287 2505 2523 2923 3717 3737 2287
Size: 218 18 400 794 20 3957
Fragments arranged by size:
3957 794 400 218 20 18
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5407
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5372
DraI TTT'AAA
Cuts at: 2433 2711 2433
Size: 278 5129
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4414 809
Size: 507 3098 1802
Fragments arranged by size:
3098 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2124 2167 2854
Size: 54 1425 50 275 18 294 43 687
Cuts at: 2854 3743 5372 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 687 294 275 54 50
43 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2167 2854 1537
Size: 275 355 687 4090
Fragments arranged by size:
4090 687 355 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3337 1353
Size: 1984 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2531 3994 4368 4514 387
Size: 1064 138 942 1463 374 146 1280
Fragments arranged by size:
1463 1280 1064 942 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3470 4067 4854 5122 1239
Size: 393 1838 597 787 268 1524
Fragments arranged by size:
1838 1524 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2716 3094 2716
Size: 378 5029
FseI GG_CCGG'CC
Cuts at: 2130 2130
Size: 5407
FspI TGC'GCA
Cuts at: 3913 3913
Size: 5407
HaeII r_GCGC'y
Cuts at: 1678 2073 2116 2394 2620 4554 1678
Size: 395 43 278 226 1934 2531
Fragments arranged by size:
2531 1934 395 278 226 43
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2523 2555 2674 2706 4048
Size: 32 135 32 951 32 119 32 1342
Cuts at: 4048 4080 4122 4154 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1342 951 135 119 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2377 2751 2997 669
Size: 988 281 439 374 246 3079
Fragments arranged by size:
3079 988 439 374 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5407
HpaI GTT'AAC
Cuts at: 2997 2997
Size: 5407
KasI G'GCGC_C
Cuts at: 2069 2069
Size: 5407
KpnI G_GTAC'C
Cuts at: 2732 2732
Size: 5407
MfeI C'AATT_G
Cuts at: 3006 3006
Size: 5407
MluI A'CGCG_T
Cuts at: 5283 5283
Size: 5407
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4521 4705 1305
Size: 3216 184 2007
Fragments arranged by size:
3216 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3585
Fragments arranged by size:
3585 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2057 3365 3724 3883 518
Size: 648 891 1308 359 159 2042
Fragments arranged by size:
2042 1308 891 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2118 2823 3500 4648 4893 1606
Size: 512 705 677 1148 245 2120
Fragments arranged by size:
2120 1148 705 677 512 245
NaeI GCC'GGC
Cuts at: 2128 2128
Size: 5407
NarI GG'CG_CC
Cuts at: 2070 2070
Size: 5407
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4855
NdeI CA'TA_TG
Cuts at: 387 2494 387
Size: 2107 3300
NgoMIV G'CCGG_C
Cuts at: 2126 2126
Size: 5407
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5407
NotI GC'GGCC_GC
Cuts at: 2854 2854
Size: 5407
NspI r_CATG'y
Cuts at: 1132 1229 2238 2765 4310 1132
Size: 97 1009 527 1545 2229
Fragments arranged by size:
2229 1545 1009 527 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5407
PciI A'CATG_T
Cuts at: 4306 4306
Size: 5407
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2620 1108
Size: 1512 3895
PmeI GTTT'AAAC
Cuts at: 2711 2711
Size: 5407
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3476 3508 4989 5021 3476
Size: 32 1481 32 3862
Fragments arranged by size:
3862 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2300 1613
Size: 687 4720
PsiI TTA'TAA
Cuts at: 2977 2977
Size: 5407
PspXI vC'TCGA_Gb
Cuts at: 2047 2047
Size: 5407
PstI C_TGCA'G
Cuts at: 830 2282 2759 830
Size: 1452 477 3478
Fragments arranged by size:
3478 1452 477
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5407
SacII CC_GC'GG
Cuts at: 2119 2119
Size: 5407
SalI G'TCGA_C
Cuts at: 1936 2749 1936
Size: 813 4594
SbfI CC_TGCA'GG
Cuts at: 2759 2759
Size: 5407
ScaI AGT'ACT
Cuts at: 3655 5367 3655
Size: 1712 3695
SfcI C'TryA_G
Cuts at: 826 1045 2278 2755 3890 4571 4762 826
Size: 219 1233 477 1135 681 191 1471
Fragments arranged by size:
1471 1233 1135 681 477 219 191
SfoI GGC'GCC
Cuts at: 2071 2071
Size: 5407
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5407
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5407
SmaI CCC'GGG
Cuts at: 1872 2734 1872
Size: 862 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2047 2174 3511 4412 4674 820
Size: 197 926 104 127 1337 901 262 1553
Fragments arranged by size:
1553 1337 926 901 262 197 127 104
SnaBI TAC'GTA
Cuts at: 493 3198 493
Size: 2705 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5407
SphI G_CATG'C
Cuts at: 1132 2238 2765 1132
Size: 1106 527 3774
Fragments arranged by size:
3774 1106 527
SspI AAT'ATT
Cuts at: 5 52 3331 5
Size: 47 3279 2081
Fragments arranged by size:
3279 2081 47
StuI AGG'CCT
Cuts at: 1517 2045 2253 1517
Size: 528 208 4671
Fragments arranged by size:
4671 528 208
StyI C'CwwG_G
Cuts at: 513 1065 2846 513
Size: 552 1781 3074
Fragments arranged by size:
3074 1781 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2683 3434 3451 3604 3789 4234 325
Size: 1255 1103 751 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1103 751 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3653 5365 96
Size: 274 80 33 51 1443 1676 1712 138
Fragments arranged by size:
1712 1676 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5367 5399 5367
Size: 32 5375
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5407
XbaI T'CTAG_A
Cuts at: 2743 2743
Size: 5407
XhoI C'TCGA_G
Cuts at: 1943 2047 1943
Size: 104 5303
XmaI C'CCGG_G
Cuts at: 1870 2732 1870
Size: 862 4545
XmnI GAAnn'nnTTC
Cuts at: 2507 3536 2507
Size: 1029 4378
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3215 276
Size: 53 83 186 936 1681 2468
Fragments arranged by size:
2468 1681 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FseI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PspXI PstI PvuI SacII SalI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoO109I EcoRV FalI FspAI NruI NsiI PacI
PfoI PmlI PpuMI PspOMI PsrI PvuII RsrII SacI
SanDI SapI SexAI SfiI SrfI SwaI XcmI