Restriction map for
pFN21AE3067
(Circular) (Six-base) MAPSORT of: pFN21AE3067.seq Check: 8440 from: 1 to: 5371
pFN21AE3067 5371 bp
With 182 enzymes: SgfI *
March 28, 2012 12:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3181 278
Size: 53 83 186 936 1645 2468
Fragments arranged by size:
2468 1645 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2714 1937
Size: 777 4594
Acc65I G'GTAC_C
Cuts at: 2692 2692
Size: 5371
AclI AA'CG_TT
Cuts at: 1563 3498 3871 1563
Size: 1935 373 3063
Fragments arranged by size:
3063 1935 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2550 3434 4818 5086 1632
Size: 918 884 1384 268 1917
Fragments arranged by size:
1917 1384 918 884 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5174
AflIII A'CryG_T
Cuts at: 1203 2335 4270 5247 1203
Size: 1132 1935 977 1327
Fragments arranged by size:
1935 1327 1132 977
AgeI A'CCGG_T
Cuts at: 4202 4202
Size: 5371
AhdI GACnn_n'nnGTC
Cuts at: 4100 4100
Size: 5371
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5371
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5371
AlwNI CAG_nnn'CTG
Cuts at: 3114 4686 3114
Size: 1572 3799
ApaI G_GGCC'C
Cuts at: 2153 2449 2487 2515 2557 2567 2153
Size: 296 38 28 42 10 4957
Fragments arranged by size:
4957 296 42 38 28 10
ApaLI G'TGCA_C
Cuts at: 3428 4584 3428
Size: 1156 4215
ApoI r'AATT_y
Cuts at: 1493 2680 2906 3058 5167 1493
Size: 1187 226 152 2109 1697
Fragments arranged by size:
2109 1697 1187 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3925 160
Size: 872 2893 1606
Fragments arranged by size:
2893 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5371
AvaI C'yCGr_G
Cuts at: 1870 1943 2378 2442 2566 2696 1870
Size: 73 435 64 124 130 4545
Fragments arranged by size:
4545 435 130 124 73 64
BamHI G'GATC_C
Cuts at: 2701 3068 2701
Size: 367 5004
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2270 2474 2692 4147 618
Size: 325 217 627 483 204 218 1455 1842
Fragments arranged by size:
1842 1455 627 483 325 218 217 204
BanII G_rGCy'C
Cuts at: 1391 2153 2449 2487 2515 2557 2567 2690 1391
Size: 762 296 38 28 42 10 123 4072
Fragments arranged by size:
4072 762 296 123 42 38 28 10
BbeI G_GCGC'C
Cuts at: 2274 2478 2274
Size: 204 5167
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4400
BbvCI CC'TCA_GC
Cuts at: 2577 2577
Size: 5371
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2417 2451 3562 3596 1903
Size: 34 480 34 1111 34 3678
Fragments arranged by size:
3678 1111 480 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3263 4473 3263
Size: 1210 4161
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5371
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2510 3982 136
Size: 107 122 71 631 605 838 1472 1525
Fragments arranged by size:
1525 1472 838 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2250 2283 5366 2250
Size: 33 3083 2255
Fragments arranged by size:
3083 2255 33
BlpI GC'TnA_GC
Cuts at: 2319 2788 2319
Size: 469 4902
Bme1580I G_kGCm'C
Cuts at: 1790 2153 2449 2487 2515 2557 2567 3432 4588
Size: 363 296 38 28 42 10 865 1156
Cuts at: 4588 1790
Size: 2573
Fragments arranged by size:
2573 1156 865 363 296 42 38 28
10
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5371
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3102 4060 442
Size: 416 53 83 399 24 1685 958 1753
Fragments arranged by size:
1753 1685 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5371
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2117 2193 4031 1239
Size: 878 76 1838 2579
Fragments arranged by size:
2579 1838 878 76
Bpu10I CC'TnA_GC
Cuts at: 2577 2577
Size: 5371
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2285 3496 4361 4659 2285
Size: 1211 865 298 2997
Fragments arranged by size:
2997 1211 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4034 882
Size: 617 2535 2219
Fragments arranged by size:
2535 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2338 3162 493
Size: 711 1134 824 2702
Fragments arranged by size:
2702 1134 824 711
BsaBI GATnn'nnATC
Cuts at: 5365 5365
Size: 5371
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2271 2475 3178 3560
Size: 53 83 186 936 738 204 703 382
Cuts at: 3560 4195 275
Size: 635 1451
Fragments arranged by size:
1451 936 738 703 635 382 204 186
83 53
BsaWI w'CCGG_w
Cuts at: 3804 4202 4476 4623 5034 3804
Size: 398 274 147 411 4141
Fragments arranged by size:
4141 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5146
Fragments arranged by size:
5146 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2129 2411 2444 1186
Size: 943 282 33 4113
Fragments arranged by size:
4113 943 282 33
BseYI C'CCAG_C
Cuts at: 2200 2323 2486 4574 5070 2200
Size: 123 163 2088 496 2501
Fragments arranged by size:
2501 2088 496 163 123
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2370 1163
Size: 573 634 4164
Fragments arranged by size:
4164 634 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2509 2821 3582 4610 1540
Size: 275 186 508 312 761 1028 2301
Fragments arranged by size:
2301 1028 761 508 312 275 186
BsiHKAI G_wGCw'C
Cuts at: 2301 3432 3517 4588 2301
Size: 1131 85 1071 3084
Fragments arranged by size:
3084 1131 1071 85
BsmI GAATG_Cn'
Cuts at: 2882 2975 2882
Size: 93 5278
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2153 2301 2449 2487 2515 2557 2567
Size: 399 363 148 148 38 28 42 10
Cuts at: 2567 2690 3432 3517 4588 1391
Size: 123 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 363 148 148 123
85 42 38 28 10
BspEI T'CCGG_A
Cuts at: 5034 5034
Size: 5371
BspHI T'CATG_A
Cuts at: 3260 4990 3260
Size: 1730 3641
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2726 844
Size: 1882 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3258 1394
Size: 429 1435 3507
Fragments arranged by size:
3507 1435 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3866 4040 65
Size: 1183 2618 174 1396
Fragments arranged by size:
2618 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2477 2504 4015 4202 1700
Size: 110 667 27 1511 187 2869
Fragments arranged by size:
2869 1511 667 187 110 27
BsrGI T'GTAC_A
Cuts at: 96 2052 96
Size: 1956 3415
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5371
BssSI C'ACGA_G
Cuts at: 3431 4443 3431
Size: 1012 4359
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5371
BstBI TT'CG_AA
Cuts at: 4175 4175
Size: 5371
BstEII G'GTnAC_C
Cuts at: 1184 2137 1184
Size: 953 4418
BstYI r'GATC_y
Cuts at: 1972 2250 2283 2463 2701 3068 3453 3470 4922
Size: 278 33 180 238 367 385 17 1452
Cuts at: 4922 5008 5020 5366 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 385 367 346 278 238 180
86 33 17 12
Bsu36I CC'TnA_GG
Cuts at: 2154 2154
Size: 5371
BtgI C'CryG_G
Cuts at: 513 1065 2467 2586 513
Size: 552 1402 119 3298
Fragments arranged by size:
3298 1402 552 119
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4632
Fragments arranged by size:
4632 683 56
BtsI GCAGTG_nn'
Cuts at: 2887 3681 3701 2887
Size: 794 20 4557
Fragments arranged by size:
4557 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5371
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5336
DraI TTT'AAA
Cuts at: 2675 2675
Size: 5371
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2135 4378 809
Size: 507 819 2243 1802
Fragments arranged by size:
2243 1802 819 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2517 2589 2628
Size: 54 1425 50 275 18 687 72 39
Cuts at: 2628 2818 3707 5336 8
Size: 190 889 1629 43
Fragments arranged by size:
1629 1425 889 687 275 190 72 54
50 43 39 18
EagI C'GGCC_G
Cuts at: 1537 1812 2818 1537
Size: 275 1006 4090
Fragments arranged by size:
4090 1006 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3301 1353
Size: 1948 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2033 3958 4332 4478 387
Size: 1064 138 444 1925 374 146 1280
Fragments arranged by size:
1925 1280 1064 444 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2117 2193 2550 3434 4031 4818 5086
Size: 393 485 76 357 884 597 787 268
Cuts at: 5086 1239
Size: 1524
Fragments arranged by size:
1524 884 787 597 485 393 357 268
76
EcoO109I rG'GnC_Cy
Cuts at: 2119 2149 2150 2160 2512 2553 2554 2563 2582
Size: 30 1 10 352 41 1 9 19
Cuts at: 2582 2119
Size: 4908
Fragments arranged by size:
4908 352 41 30 19 10 9 1
1
EcoRI G'AATT_C
Cuts at: 2680 3058 2680
Size: 378 4993
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2254 2254
Size: 5371
FspI TGC'GCA
Cuts at: 3877 3877
Size: 5371
HaeII r_GCGC'y
Cuts at: 1678 2274 2478 2660 4518 1678
Size: 596 204 182 1858 2531
Fragments arranged by size:
2531 1858 596 204 182
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4012 4044 4086 4118 1373
Size: 32 135 32 2440 32 42 32 2626
Fragments arranged by size:
2626 2440 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2293 2715 2961 669
Size: 988 281 355 422 246 3079
Fragments arranged by size:
3079 988 422 355 281 246
HindIII A'AGCT_T
Cuts at: 748 2532 748
Size: 1784 3587
HpaI GTT'AAC
Cuts at: 2961 2961
Size: 5371
KasI G'GCGC_C
Cuts at: 2270 2474 2270
Size: 204 5167
KpnI G_GTAC'C
Cuts at: 2696 2696
Size: 5371
MfeI C'AATT_G
Cuts at: 2970 2970
Size: 5371
MluI A'CGCG_T
Cuts at: 5247 5247
Size: 5371
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2037 2356 4485 4669 1305
Size: 732 319 2129 184 2007
Fragments arranged by size:
2129 2007 732 319 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2519 2591 10
Size: 54 1425 343 687 72 2790
Fragments arranged by size:
2790 1425 687 343 72 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3329 3688 3847 518
Size: 648 2163 359 159 2042
Fragments arranged by size:
2163 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2490 2787 3464 4612 4857 1606
Size: 884 297 677 1148 245 2120
Fragments arranged by size:
2120 1148 884 677 297 245
NaeI GCC'GGC
Cuts at: 2479 2479
Size: 5371
NarI GG'CG_CC
Cuts at: 2271 2475 2271
Size: 204 5167
NcoI C'CATG_G
Cuts at: 513 1065 2586 513
Size: 552 1521 3298
Fragments arranged by size:
3298 1521 552
NdeI CA'TA_TG
Cuts at: 387 2167 387
Size: 1780 3591
NgoMIV G'CCGG_C
Cuts at: 2477 2477
Size: 5371
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5371
NotI GC'GGCC_GC
Cuts at: 2818 2818
Size: 5371
NspI r_CATG'y
Cuts at: 1132 1229 2603 2729 4274 1132
Size: 97 1374 126 1545 2229
Fragments arranged by size:
2229 1545 1374 126 97
PasI CC'CwG_GG
Cuts at: 1348 2549 2558 1348
Size: 1201 9 4161
Fragments arranged by size:
4161 1201 9
PciI A'CATG_T
Cuts at: 4270 4270
Size: 5371
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2090 2171 1108
Size: 982 81 4308
Fragments arranged by size:
4308 982 81
PmeI GTTT'AAAC
Cuts at: 2675 2675
Size: 5371
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3440 3472 4953 4985 3440
Size: 32 1481 32 3826
Fragments arranged by size:
3826 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2119 2160 2582 2119
Size: 41 422 4908
Fragments arranged by size:
4908 422 41
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5371
PsiI TTA'TAA
Cuts at: 2941 2941
Size: 5371
PspOMI G'GGCC_C
Cuts at: 2149 2445 2483 2511 2553 2563 2149
Size: 296 38 28 42 10 4957
Fragments arranged by size:
4957 296 42 38 28 10
PstI C_TGCA'G
Cuts at: 830 2723 830
Size: 1893 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5371
SalI G'TCGA_C
Cuts at: 1936 2713 1936
Size: 777 4594
SanDI GG'GwC_CC
Cuts at: 2160 2582 2160
Size: 422 4949
SbfI CC_TGCA'GG
Cuts at: 2723 2723
Size: 5371
ScaI AGT'ACT
Cuts at: 3619 5331 3619
Size: 1712 3659
SfcI C'TryA_G
Cuts at: 826 1045 2719 3854 4535 4726 826
Size: 219 1674 1135 681 191 1471
Fragments arranged by size:
1674 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2272 2476 2272
Size: 204 5167
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5371
SgrAI Cr'CCGG_yG
Cuts at: 1700 2477 1700
Size: 777 4594
SmaI CCC'GGG
Cuts at: 1872 2568 2698 1872
Size: 696 130 4545
Fragments arranged by size:
4545 696 130
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2300 3475 4376 4638 820
Size: 197 926 357 1175 901 262 1553
Fragments arranged by size:
1553 1175 926 901 357 262 197
SnaBI TAC'GTA
Cuts at: 493 3162 493
Size: 2669 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5371
SphI G_CATG'C
Cuts at: 1132 2603 2729 1132
Size: 1471 126 3774
Fragments arranged by size:
3774 1471 126
SspI AAT'ATT
Cuts at: 5 52 3295 5
Size: 47 3243 2081
Fragments arranged by size:
3243 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5371
StyI C'CwwG_G
Cuts at: 513 1065 2229 2586 2810 513
Size: 552 1164 357 224 3074
Fragments arranged by size:
3074 1164 552 357 224
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2242 3398 3415 3568 3753 4198 325
Size: 1255 662 1156 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1156 662 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2052 3617 5329
Size: 274 80 33 51 1443 75 1565 1712
Cuts at: 5329 96
Size: 138
Fragments arranged by size:
1712 1565 1443 274 138 80 75 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5331 5363 5331
Size: 32 5339
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5371
XbaI T'CTAG_A
Cuts at: 2707 2707
Size: 5371
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5371
XmaI C'CCGG_G
Cuts at: 1870 2566 2696 1870
Size: 696 130 4545
Fragments arranged by size:
4545 696 130
XmnI GAAnn'nnTTC
Cuts at: 2210 3500 2210
Size: 1290 4081
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3179 276
Size: 53 83 186 936 1645 2468
Fragments arranged by size:
2468 1645 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV
FseI FspAI NruI NsiI PacI PfoI PmlI PspXI
PsrI PvuII RsrII SacI SacII SapI SexAI SfiI
SrfI SwaI XcmI