Restriction map for
pFN21AE3031
(Circular) (Six-base) MAPSORT of: pFN21AE3031.seq Check: 2978 from: 1 to: 5173
pFN21AE3031 5173 bp
With 182 enzymes: SgfI *
December 28, 2011 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2983 278
Size: 53 83 186 936 1447 2468
Fragments arranged by size:
2468 1447 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2516 1937
Size: 579 4594
Acc65I G'GTAC_C
Cuts at: 2494 2494
Size: 5173
AclI AA'CG_TT
Cuts at: 1563 3300 3673 1563
Size: 1737 373 3063
Fragments arranged by size:
3063 1737 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3236 4620 4888 1632
Size: 1604 1384 268 1917
Fragments arranged by size:
1917 1604 1384 268
AfeI AGC'GCT
Cuts at: 2255 2255
Size: 5173
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4976
AflIII A'CryG_T
Cuts at: 1203 4072 5049 1203
Size: 2869 977 1327
Fragments arranged by size:
2869 1327 977
AgeI A'CCGG_T
Cuts at: 4004 4004
Size: 5173
AhdI GACnn_n'nnGTC
Cuts at: 3902 3902
Size: 5173
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5173
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5173
AlwNI CAG_nnn'CTG
Cuts at: 2916 4488 2916
Size: 1572 3601
ApaLI G'TGCA_C
Cuts at: 3230 4386 3230
Size: 1156 4017
ApoI r'AATT_y
Cuts at: 1493 2482 2708 2860 4969 1493
Size: 989 226 152 2109 1697
Fragments arranged by size:
2109 1697 989 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3727 160
Size: 872 2695 1606
Fragments arranged by size:
2695 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5173
AvaI C'yCGr_G
Cuts at: 1870 1943 2016 2349 2447 2498 1870
Size: 73 73 333 98 51 4545
Fragments arranged by size:
4545 333 98 73 73 51
BamHI G'GATC_C
Cuts at: 2503 2870 2503
Size: 367 4806
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2034 2443 2494 3949 618
Size: 325 217 627 247 409 51 1455 1842
Fragments arranged by size:
1842 1455 627 409 325 247 217 51
BanII G_rGCy'C
Cuts at: 1391 2064 2492 1391
Size: 673 428 4072
Fragments arranged by size:
4072 673 428
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4202
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3364 3398 1903
Size: 34 50 34 1343 34 3678
Fragments arranged by size:
3678 1343 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3065 4275 3065
Size: 1210 3963
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5173
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2183 3784 136
Size: 107 122 71 631 605 511 1601 1525
Fragments arranged by size:
1601 1525 631 605 511 122 107 71
BglII A'GATC_T
Cuts at: 2424 5168 2424
Size: 2744 2429
BlpI GC'TnA_GC
Cuts at: 2590 2590
Size: 5173
Bme1580I G_kGCm'C
Cuts at: 1790 3234 4390 1790
Size: 1444 1156 2573
Fragments arranged by size:
2573 1444 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5173
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2236 2904 3862
Size: 416 53 83 399 24 819 668 958
Cuts at: 3862 442
Size: 1753
Fragments arranged by size:
1753 958 819 668 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5173
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2048 2219 3833 1239
Size: 809 171 1614 2579
Fragments arranged by size:
2579 1614 809 171
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3298 4163 4461 3298
Size: 865 298 4010
Fragments arranged by size:
4010 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2332 3836 882
Size: 617 833 1504 2219
Fragments arranged by size:
2219 1504 833 617
BsaAI yAC'GTr
Cuts at: 493 1204 2964 493
Size: 711 1760 2702
Fragments arranged by size:
2702 1760 711
BsaBI GATnn'nnATC
Cuts at: 5167 5167
Size: 5173
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2980 3362 3997 275
Size: 53 83 186 936 1447 382 635 1451
Fragments arranged by size:
1451 1447 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2430 3606 4004 4278 4425 4836 2430
Size: 1176 398 274 147 411 2767
Fragments arranged by size:
2767 1176 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4948
Fragments arranged by size:
4948 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2057 1186
Size: 871 4302
BseYI C'CCAG_C
Cuts at: 4376 4872 4376
Size: 496 4677
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4600
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2263 2623 3384 4412 1540
Size: 275 186 262 360 761 1028 2301
Fragments arranged by size:
2301 1028 761 360 275 262 186
BsiHKAI G_wGCw'C
Cuts at: 2017 2064 3234 3319 4390 2017
Size: 47 1170 85 1071 2800
Fragments arranged by size:
2800 1170 1071 85 47
BsmI GAATG_Cn'
Cuts at: 2684 2777 2684
Size: 93 5080
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2017 2064 2492 3234 3319 4390 1391
Size: 399 227 47 428 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 428 399 227 85 47
BspEI T'CCGG_A
Cuts at: 4836 4836
Size: 5173
BspHI T'CATG_A
Cuts at: 3062 4792 3062
Size: 1730 3443
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2461 2528 844
Size: 1617 67 3489
Fragments arranged by size:
3489 1617 67
BsrBI CCG'CTC
Cuts at: 1394 1823 3060 1394
Size: 429 1237 3507
Fragments arranged by size:
3507 1237 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2364 3668 3842 65
Size: 1183 1116 1304 174 1396
Fragments arranged by size:
1396 1304 1183 1116 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2031 3817 4004 1700
Size: 110 221 1786 187 2869
Fragments arranged by size:
2869 1786 221 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5173
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5173
BssSI C'ACGA_G
Cuts at: 3233 4245 3233
Size: 1012 4161
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5173
BstBI TT'CG_AA
Cuts at: 3977 3977
Size: 5173
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5173
BstYI r'GATC_y
Cuts at: 1972 2424 2503 2870 3255 3272 4724 4810 4822
Size: 452 79 367 385 17 1452 86 12
Cuts at: 4822 5168 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 452 385 367 346 86 79
17 12
Bsu36I CC'TnA_GG
Cuts at: 2040 2040
Size: 5173
BtgI C'CryG_G
Cuts at: 513 1065 2187 513
Size: 552 1122 3499
Fragments arranged by size:
3499 1122 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4434
Fragments arranged by size:
4434 683 56
BtsI GCAGTG_nn'
Cuts at: 2689 3483 3503 2689
Size: 794 20 4359
Fragments arranged by size:
4359 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5173
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5138
DraI TTT'AAA
Cuts at: 2477 2477
Size: 5173
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4180 809
Size: 507 2864 1802
Fragments arranged by size:
2864 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2184 2620 3509
Size: 54 1425 50 275 18 354 436 889
Cuts at: 3509 5138 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 436 354 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2620 1537
Size: 275 808 4090
Fragments arranged by size:
4090 808 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2231 3103 1353
Size: 878 872 3423
Fragments arranged by size:
3423 878 872
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3760 4134 4280 387
Size: 1064 138 2171 374 146 1280
Fragments arranged by size:
2171 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2062 2062
Size: 5173
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2048 2219 3236 3833 4620 4888 1239
Size: 393 416 171 1017 597 787 268 1524
Fragments arranged by size:
1524 1017 787 597 416 393 268 171
EcoRI G'AATT_C
Cuts at: 2482 2860 2482
Size: 378 4795
FspI TGC'GCA
Cuts at: 3679 3679
Size: 5173
HaeII r_GCGC'y
Cuts at: 1678 2195 2257 4320 1678
Size: 517 62 2063 2531
Fragments arranged by size:
2531 2063 517 62
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3814 3846 3888 3920 1373
Size: 32 135 32 2242 32 42 32 2626
Fragments arranged by size:
2626 2242 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2517 2763 669
Size: 988 281 579 246 3079
Fragments arranged by size:
3079 988 579 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5173
HpaI GTT'AAC
Cuts at: 2763 2763
Size: 5173
KpnI G_GTAC'C
Cuts at: 2498 2498
Size: 5173
MfeI C'AATT_G
Cuts at: 2772 2772
Size: 5173
MluI A'CGCG_T
Cuts at: 5049 5049
Size: 5173
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4287 4471 1305
Size: 2982 184 2007
Fragments arranged by size:
2982 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3351
Fragments arranged by size:
3351 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3131 3490 3649 518
Size: 648 1965 359 159 2042
Fragments arranged by size:
2042 1965 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2189 2339 2589 3266 4414 4659 1606
Size: 583 150 250 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 583 250 245 150
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4621
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5173
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5173
NotI GC'GGCC_GC
Cuts at: 2620 2620
Size: 5173
NspI r_CATG'y
Cuts at: 1132 1229 2531 4076 1132
Size: 97 1302 1545 2229
Fragments arranged by size:
2229 1545 1302 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5173
PciI A'CATG_T
Cuts at: 4072 4072
Size: 5173
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5173
PmeI GTTT'AAAC
Cuts at: 2477 2477
Size: 5173
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3242 3274 4755 4787 3242
Size: 32 1481 32 3628
Fragments arranged by size:
3628 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5173
PsiI TTA'TAA
Cuts at: 2743 2743
Size: 5173
PstI C_TGCA'G
Cuts at: 830 2525 830
Size: 1695 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5173
PvuII CAG'CTG
Cuts at: 2339 2339
Size: 5173
SacI G_AGCT'C
Cuts at: 2064 2064
Size: 5173
SacII CC_GC'GG
Cuts at: 2190 2190
Size: 5173
SalI G'TCGA_C
Cuts at: 1936 2515 1936
Size: 579 4594
SbfI CC_TGCA'GG
Cuts at: 2525 2525
Size: 5173
ScaI AGT'ACT
Cuts at: 2246 3421 5133 2246
Size: 1175 1712 2286
Fragments arranged by size:
2286 1712 1175
SfcI C'TryA_G
Cuts at: 826 1045 2521 3656 4337 4528 826
Size: 219 1476 1135 681 191 1471
Fragments arranged by size:
1476 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5173
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5173
SmaI CCC'GGG
Cuts at: 1872 2351 2449 2500 1872
Size: 479 98 51 4545
Fragments arranged by size:
4545 479 98 51
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2016 3277 4178 4440 820
Size: 197 926 73 1261 901 262 1553
Fragments arranged by size:
1553 1261 926 901 262 197 73
SnaBI TAC'GTA
Cuts at: 493 2964 493
Size: 2471 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5173
SphI G_CATG'C
Cuts at: 1132 2531 1132
Size: 1399 3774
SspI AAT'ATT
Cuts at: 5 52 3097 5
Size: 47 3045 2081
Fragments arranged by size:
3045 2081 47
StuI AGG'CCT
Cuts at: 1517 2087 1517
Size: 570 4603
StyI C'CwwG_G
Cuts at: 513 1065 2612 513
Size: 552 1547 3074
Fragments arranged by size:
3074 1547 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2255 2277 3200 3217 3370 3555 4000
Size: 1255 675 22 923 17 153 185 445
Cuts at: 4000 325
Size: 1498
Fragments arranged by size:
1498 1255 923 675 445 185 153 22
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2244 3419 5131
Size: 274 80 33 51 1443 267 1175 1712
Cuts at: 5131 96
Size: 138
Fragments arranged by size:
1712 1443 1175 274 267 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2205 2237 5133 5165 2205
Size: 32 2896 32 2213
Fragments arranged by size:
2896 2213 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5173
XbaI T'CTAG_A
Cuts at: 2509 2509
Size: 5173
XhoI C'TCGA_G
Cuts at: 1943 2016 1943
Size: 73 5100
XmaI C'CCGG_G
Cuts at: 1870 2349 2447 2498 1870
Size: 479 98 51 4545
Fragments arranged by size:
4545 479 98 51
XmnI GAAnn'nnTTC
Cuts at: 3302 3302
Size: 5173
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2981 276
Size: 53 83 186 936 1447 2468
Fragments arranged by size:
2468 1447 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI
SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII
EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI RsrII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI