Restriction map for
pFN21AE3028
(Circular) (Six-base) MAPSORT of: pFN21AE3028.seq Check: 4674 from: 1 to: 5158
pFN21AE3028 5158 bp
With 182 enzymes: SgfI *
March 28, 2012 14:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2968 278
Size: 53 83 186 936 1432 2468
Fragments arranged by size:
2468 1432 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2501 1937
Size: 564 4594
Acc65I G'GTAC_C
Cuts at: 2479 2479
Size: 5158
AclI AA'CG_TT
Cuts at: 1563 3285 3658 1563
Size: 1722 373 3063
Fragments arranged by size:
3063 1722 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3221 4605 4873 1632
Size: 1589 1384 268 1917
Fragments arranged by size:
1917 1589 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4961
AflIII A'CryG_T
Cuts at: 1203 4057 5034 1203
Size: 2854 977 1327
Fragments arranged by size:
2854 1327 977
AgeI A'CCGG_T
Cuts at: 3989 3989
Size: 5158
AhdI GACnn_n'nnGTC
Cuts at: 3887 3887
Size: 5158
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5158
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5158
AlwNI CAG_nnn'CTG
Cuts at: 2147 2901 4473 2147
Size: 754 1572 2832
Fragments arranged by size:
2832 1572 754
ApaLI G'TGCA_C
Cuts at: 3215 4371 3215
Size: 1156 4002
ApoI r'AATT_y
Cuts at: 1493 2467 2693 2845 4954 1493
Size: 974 226 152 2109 1697
Fragments arranged by size:
2109 1697 974 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3712 160
Size: 872 2680 1606
Fragments arranged by size:
2680 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5158
AvaI C'yCGr_G
Cuts at: 1870 1943 2030 2483 1870
Size: 73 87 453 4545
Fragments arranged by size:
4545 453 87 73
BamHI G'GATC_C
Cuts at: 2488 2855 2488
Size: 367 4791
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2153 2314 2479 3934 618
Size: 325 217 627 366 161 165 1455 1842
Fragments arranged by size:
1842 1455 627 366 325 217 165 161
BanII G_rGCy'C
Cuts at: 1391 2477 1391
Size: 1086 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2136 928
Size: 971 237 3950
Fragments arranged by size:
3950 971 237
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3349 3383 1903
Size: 34 1412 34 3678
Fragments arranged by size:
3678 1412 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3050 4260 3050
Size: 1210 3948
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5158
BfrBI ATG'CAT
Cuts at: 2322 2322
Size: 5158
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 3769 136
Size: 107 122 71 631 605 337 1760 1525
Fragments arranged by size:
1760 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 2026 5153 2026
Size: 3127 2031
BlpI GC'TnA_GC
Cuts at: 2575 2575
Size: 5158
Bme1580I G_kGCm'C
Cuts at: 1790 2156 3219 4375 1790
Size: 366 1063 1156 2573
Fragments arranged by size:
2573 1156 1063 366
BmgBI CAC'GTC
Cuts at: 1318 2134 1318
Size: 816 4342
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2889 3847 442
Size: 416 53 83 399 24 1472 958 1753
Fragments arranged by size:
1753 1472 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5158
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3818 1239
Size: 2579 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3283 4148 4446 3283
Size: 865 298 3995
Fragments arranged by size:
3995 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2079 3821 882
Size: 617 580 1742 2219
Fragments arranged by size:
2219 1742 617 580
BsaAI yAC'GTr
Cuts at: 493 1204 2949 493
Size: 711 1745 2702
Fragments arranged by size:
2702 1745 711
BsaBI GATnn'nnATC
Cuts at: 2342 5152 2342
Size: 2810 2348
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2247 2965 3347 3982
Size: 53 83 186 936 714 718 382 635
Cuts at: 3982 275
Size: 1451
Fragments arranged by size:
1451 936 718 714 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3591 3989 4263 4410 4821 3591
Size: 398 274 147 411 3928
Fragments arranged by size:
3928 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4933
Fragments arranged by size:
4933 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5158
BseYI C'CCAG_C
Cuts at: 2242 4361 4857 2242
Size: 2119 496 2543
Fragments arranged by size:
2543 2119 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4585
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2608 3369 4397 1540
Size: 275 186 607 761 1028 2301
Fragments arranged by size:
2301 1028 761 607 275 186
BsiHKAI G_wGCw'C
Cuts at: 2022 3219 3304 4375 2022
Size: 1197 85 1071 2805
Fragments arranged by size:
2805 1197 1071 85
BsmI GAATG_Cn'
Cuts at: 2669 2762 2669
Size: 93 5065
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2022 2156 2477 3219 3304 4375 1391
Size: 399 232 134 321 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 321 232 134 85
BspEI T'CCGG_A
Cuts at: 4821 4821
Size: 5158
BspHI T'CATG_A
Cuts at: 3047 4777 3047
Size: 1730 3428
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2513 844
Size: 1669 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3045 1394
Size: 429 1222 3507
Fragments arranged by size:
3507 1222 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3653 3827 65
Size: 1183 2405 174 1396
Fragments arranged by size:
2405 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3802 3989 1700
Size: 110 1992 187 2869
Fragments arranged by size:
2869 1992 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5158
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5158
BssSI C'ACGA_G
Cuts at: 3218 4230 3218
Size: 1012 4146
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2388 794
Size: 1594 3564
BstBI TT'CG_AA
Cuts at: 3962 3962
Size: 5158
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5158
BstXI CCAn_nnnn'nTGG
Cuts at: 2242 2242
Size: 5158
BstYI r'GATC_y
Cuts at: 1972 2026 2343 2488 2855 3240 3257 4709 4795
Size: 54 317 145 367 385 17 1452 86
Cuts at: 4795 4807 5153 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 385 367 346 317 145 86
54 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4606
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4419
Fragments arranged by size:
4419 683 56
BtsI GCAGTG_nn'
Cuts at: 2056 2674 3468 3488 2056
Size: 618 794 20 3726
Fragments arranged by size:
3726 794 618 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5158
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5123
DraI TTT'AAA
Cuts at: 2462 2462
Size: 5158
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4165 809
Size: 507 2849 1802
Fragments arranged by size:
2849 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2605 3494 5123
Size: 54 1425 50 275 18 775 889 1629
Cuts at: 5123 8
Size: 43
Fragments arranged by size:
1629 1425 889 775 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2605 1537
Size: 275 793 4090
Fragments arranged by size:
4090 793 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2010 2405 3088 1353
Size: 657 395 683 3423
Fragments arranged by size:
3423 683 657 395
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3745 4119 4265 387
Size: 1064 138 2156 374 146 1280
Fragments arranged by size:
2156 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3221 3818 4605 4873 1239
Size: 393 1589 597 787 268 1524
Fragments arranged by size:
1589 1524 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2414 2414
Size: 5158
EcoRI G'AATT_C
Cuts at: 2467 2845 2467
Size: 378 4780
EcoRV GAT'ATC
Cuts at: 2053 2053
Size: 5158
FspI TGC'GCA
Cuts at: 3664 3664
Size: 5158
HaeII r_GCGC'y
Cuts at: 1678 4305 1678
Size: 2627 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3799 3831 3873 3905 1373
Size: 32 135 32 2227 32 42 32 2626
Fragments arranged by size:
2626 2227 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2502 2748 669
Size: 988 281 564 246 3079
Fragments arranged by size:
3079 988 564 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5158
HpaI GTT'AAC
Cuts at: 2748 2748
Size: 5158
KpnI G_GTAC'C
Cuts at: 2483 2483
Size: 5158
MfeI C'AATT_G
Cuts at: 2757 2757
Size: 5158
MluI A'CGCG_T
Cuts at: 5034 5034
Size: 5158
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4272 4456 1305
Size: 2967 184 2007
Fragments arranged by size:
2967 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3336
Fragments arranged by size:
3336 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2435 3116 3475 3634 518
Size: 648 1269 681 359 159 2042
Fragments arranged by size:
2042 1269 681 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2574 3251 4399 4644 1606
Size: 968 677 1148 245 2120
Fragments arranged by size:
2120 1148 968 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4606
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5158
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5158
NotI GC'GGCC_GC
Cuts at: 2605 2605
Size: 5158
NsiI A_TGCA'T
Cuts at: 2324 2324
Size: 5158
NspI r_CATG'y
Cuts at: 1132 1229 2516 4061 1132
Size: 97 1287 1545 2229
Fragments arranged by size:
2229 1545 1287 97
PasI CC'CwG_GG
Cuts at: 1348 2410 1348
Size: 1062 4096
PciI A'CATG_T
Cuts at: 4057 4057
Size: 5158
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2214 1108
Size: 1106 4052
PmeI GTTT'AAAC
Cuts at: 2462 2462
Size: 5158
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3227 3259 4740 4772 3227
Size: 32 1481 32 3613
Fragments arranged by size:
3613 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5158
PsiI TTA'TAA
Cuts at: 2728 2728
Size: 5158
PstI C_TGCA'G
Cuts at: 830 2426 2510 830
Size: 1596 84 3478
Fragments arranged by size:
3478 1596 84
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5158
SalI G'TCGA_C
Cuts at: 1936 2500 1936
Size: 564 4594
SapI GCTCTTCn'nnn_
Cuts at: 2010 2010
Size: 5158
SbfI CC_TGCA'GG
Cuts at: 2510 2510
Size: 5158
ScaI AGT'ACT
Cuts at: 2213 3406 5118 2213
Size: 1193 1712 2253
Fragments arranged by size:
2253 1712 1193
SexAI A'CCwGG_T
Cuts at: 2073 2073
Size: 5158
SfcI C'TryA_G
Cuts at: 826 1045 2158 2422 2506 3641 4322 4513 826
Size: 219 1113 264 84 1135 681 191 1471
Fragments arranged by size:
1471 1135 1113 681 264 219 191 84
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5158
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5158
SmaI CCC'GGG
Cuts at: 1872 2485 1872
Size: 613 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2030 3262 4163 4425 820
Size: 197 926 87 1232 901 262 1553
Fragments arranged by size:
1553 1232 926 901 262 197 87
SnaBI TAC'GTA
Cuts at: 493 2949 493
Size: 2456 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5158
SphI G_CATG'C
Cuts at: 1132 2516 1132
Size: 1384 3774
SspI AAT'ATT
Cuts at: 5 52 3082 5
Size: 47 3030 2081
Fragments arranged by size:
3030 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5158
StyI C'CwwG_G
Cuts at: 513 1065 2597 513
Size: 552 1532 3074
Fragments arranged by size:
3074 1532 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3185 3202 3355 3540 3985 325
Size: 1255 1605 17 153 185 445 1498
Fragments arranged by size:
1605 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2211 3404 5116
Size: 274 80 33 51 1443 234 1193 1712
Cuts at: 5116 96
Size: 138
Fragments arranged by size:
1712 1443 1193 274 234 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2361 2393 5118 5150 2361
Size: 32 2725 32 2369
Fragments arranged by size:
2725 2369 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2111 1316
Size: 795 4363
XbaI T'CTAG_A
Cuts at: 2494 2494
Size: 5158
XhoI C'TCGA_G
Cuts at: 1943 2030 1943
Size: 87 5071
XmaI C'CCGG_G
Cuts at: 1870 2483 1870
Size: 613 4545
XmnI GAAnn'nnTTC
Cuts at: 3287 3287
Size: 5158
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2966 276
Size: 53 83 186 936 1432 2468
Fragments arranged by size:
2468 1432 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoRI EcoRV FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoO109I FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SfiI SfoI
SrfI SwaI XcmI