Restriction map for
pFN21AE3020
(Circular) (Six-base) MAPSORT of: pFN21AE3020.seq Check: 4389 from: 1 to: 5080
pFN21AE3020 5080 bp
With 182 enzymes: SgfI *
December 8, 2014 13:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2274 2890 278
Size: 53 83 186 936 738 616 2468
Fragments arranged by size:
2468 936 738 616 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2423 1937
Size: 486 4594
Acc65I G'GTAC_C
Cuts at: 2401 2401
Size: 5080
AclI AA'CG_TT
Cuts at: 1563 3207 3580 1563
Size: 1644 373 3063
Fragments arranged by size:
3063 1644 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3143 4527 4795 1632
Size: 1511 1384 268 1917
Fragments arranged by size:
1917 1511 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4883
AflIII A'CryG_T
Cuts at: 1203 3979 4956 1203
Size: 2776 977 1327
Fragments arranged by size:
2776 1327 977
AgeI A'CCGG_T
Cuts at: 3911 3911
Size: 5080
AhdI GACnn_n'nnGTC
Cuts at: 3809 3809
Size: 5080
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5080
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2383 1184
Size: 1199 3881
AlwNI CAG_nnn'CTG
Cuts at: 2823 4395 2823
Size: 1572 3508
ApaLI G'TGCA_C
Cuts at: 3137 4293 3137
Size: 1156 3924
ApoI r'AATT_y
Cuts at: 1493 2389 2615 2767 4876 1493
Size: 896 226 152 2109 1697
Fragments arranged by size:
2109 1697 896 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3634 160
Size: 872 2602 1606
Fragments arranged by size:
2602 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5080
AvaI C'yCGr_G
Cuts at: 1870 1943 2197 2405 1870
Size: 73 254 208 4545
Fragments arranged by size:
4545 254 208 73
BamHI G'GATC_C
Cuts at: 2410 2777 2410
Size: 367 4713
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2059 2210 2401 3856 618
Size: 325 217 627 272 151 191 1455 1842
Fragments arranged by size:
1842 1455 627 325 272 217 191 151
BanII G_rGCy'C
Cuts at: 1391 2399 1391
Size: 1008 4072
BbeI G_GCGC'C
Cuts at: 2063 2214 2063
Size: 151 4929
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2335 928
Size: 971 436 3673
Fragments arranged by size:
3673 971 436
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3271 3305 1903
Size: 34 1334 34 3678
Fragments arranged by size:
3678 1334 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2972 4182 2972
Size: 1210 3870
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5080
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2039 3691 136
Size: 107 122 71 631 605 367 1652 1525
Fragments arranged by size:
1652 1525 631 605 367 122 107 71
BglII A'GATC_T
Cuts at: 5075 5075
Size: 5080
BlpI GC'TnA_GC
Cuts at: 2497 2497
Size: 5080
Bme1580I G_kGCm'C
Cuts at: 1790 2100 3141 4297 1790
Size: 310 1041 1156 2573
Fragments arranged by size:
2573 1156 1041 310
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5080
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2811 3769 442
Size: 416 53 83 399 24 1394 958 1753
Fragments arranged by size:
1753 1394 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5080
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3740 1239
Size: 2501 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3205 4070 4368 3205
Size: 865 298 3917
Fragments arranged by size:
3917 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3743 882
Size: 617 2244 2219
Fragments arranged by size:
2244 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2871 493
Size: 711 1667 2702
Fragments arranged by size:
2702 1667 711
BsaBI GATnn'nnATC
Cuts at: 5074 5074
Size: 5080
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2060 2211 2271 2887
Size: 53 83 186 936 527 151 60 616
Cuts at: 2887 3269 3904 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 635 616 527 382 186 151
83 60 53
BsaWI w'CCGG_w
Cuts at: 3513 3911 4185 4332 4743 3513
Size: 398 274 147 411 3850
Fragments arranged by size:
3850 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4855
Fragments arranged by size:
4855 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5080
BseYI C'CCAG_C
Cuts at: 2172 4283 4779 2172
Size: 2111 496 2473
Fragments arranged by size:
2473 2111 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2216 1163
Size: 573 480 4027
Fragments arranged by size:
4027 573 480
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2031 2314 2530 3291 4319 1540
Size: 275 186 30 283 216 761 1028 2301
Fragments arranged by size:
2301 1028 761 283 275 216 186 30
BsiHKAI G_wGCw'C
Cuts at: 3141 3226 4297 3141
Size: 85 1071 3924
Fragments arranged by size:
3924 1071 85
BsiWI C'GTAC_G
Cuts at: 2157 2157
Size: 5080
BsmI GAATG_Cn'
Cuts at: 2591 2684 2591
Size: 93 4987
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2100 2399 3141 3226 4297 1391
Size: 399 310 299 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 310 299 85
BspEI T'CCGG_A
Cuts at: 4743 4743
Size: 5080
BspHI T'CATG_A
Cuts at: 2969 4699 2969
Size: 1730 3350
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2139 2292 2435 844
Size: 1295 153 143 3489
Fragments arranged by size:
3489 1295 153 143
BsrBI CCG'CTC
Cuts at: 1394 1823 2299 2967 1394
Size: 429 476 668 3507
Fragments arranged by size:
3507 668 476 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3575 3749 65
Size: 1183 2327 174 1396
Fragments arranged by size:
2327 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2207 3724 3911 1700
Size: 110 397 1517 187 2869
Fragments arranged by size:
2869 1517 397 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5080
BssHII G'CGCG_C
Cuts at: 1925 2356 1925
Size: 431 4649
BssSI C'ACGA_G
Cuts at: 3140 4152 3140
Size: 1012 4068
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5080
BstBI TT'CG_AA
Cuts at: 3884 3884
Size: 5080
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5080
BstYI r'GATC_y
Cuts at: 1972 2410 2777 3162 3179 4631 4717 4729 5075
Size: 438 367 385 17 1452 86 12 346
Cuts at: 5075 1972
Size: 1977
Fragments arranged by size:
1977 1452 438 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2124 2314 513
Size: 552 939 120 190 3279
Fragments arranged by size:
3279 939 552 190 120
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4341
Fragments arranged by size:
4341 683 56
BtsI GCAGTG_nn'
Cuts at: 2596 3390 3410 2596
Size: 794 20 4266
Fragments arranged by size:
4266 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5080
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5045
DraI TTT'AAA
Cuts at: 2384 2384
Size: 5080
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4087 809
Size: 507 2771 1802
Fragments arranged by size:
2771 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2028 2311 2527
Size: 54 1425 50 275 18 198 283 216
Cuts at: 2527 3416 5045 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 283 275 216 198 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2028 2311 2527 1537
Size: 275 216 283 216 4090
Fragments arranged by size:
4090 283 275 216 216
EarI CTCTTCn'nnn_
Cuts at: 1353 3010 1353
Size: 1657 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2204 3667 4041 4187 387
Size: 1064 138 615 1463 374 146 1280
Fragments arranged by size:
1463 1280 1064 615 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3143 3740 4527 4795 1239
Size: 393 1511 597 787 268 1524
Fragments arranged by size:
1524 1511 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2151 2319 2151
Size: 168 4912
EcoRI G'AATT_C
Cuts at: 2389 2767 2389
Size: 378 4702
FspI TGC'GCA
Cuts at: 3586 3586
Size: 5080
HaeII r_GCGC'y
Cuts at: 1678 2045 2063 2170 2214 4227 1678
Size: 367 18 107 44 2013 2531
Fragments arranged by size:
2531 2013 367 107 44 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3721 3753 3795 3827 1373
Size: 32 135 32 2149 32 42 32 2626
Fragments arranged by size:
2626 2149 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2424 2670 669
Size: 988 281 486 246 3079
Fragments arranged by size:
3079 988 486 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5080
HpaI GTT'AAC
Cuts at: 2670 2670
Size: 5080
KasI G'GCGC_C
Cuts at: 2059 2210 2059
Size: 151 4929
KpnI G_GTAC'C
Cuts at: 2405 2405
Size: 5080
MfeI C'AATT_G
Cuts at: 2679 2679
Size: 5080
MluI A'CGCG_T
Cuts at: 4956 4956
Size: 5080
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4194 4378 1305
Size: 2889 184 2007
Fragments arranged by size:
2889 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3258
Fragments arranged by size:
3258 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3038 3397 3556 518
Size: 648 1872 359 159 2042
Fragments arranged by size:
2042 1872 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2090 2126 2316 2496 3173 4321 4566 1606
Size: 484 36 190 180 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 484 245 190 180 36
NaeI GCC'GGC
Cuts at: 2209 2209
Size: 5080
NarI GG'CG_CC
Cuts at: 2060 2211 2060
Size: 151 4929
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3589
Fragments arranged by size:
3589 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5080
NgoMIV G'CCGG_C
Cuts at: 2207 2207
Size: 5080
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5080
NotI GC'GGCC_GC
Cuts at: 2028 2527 2028
Size: 499 4581
NspI r_CATG'y
Cuts at: 1132 1229 2438 3983 1132
Size: 97 1209 1545 2229
Fragments arranged by size:
2229 1545 1209 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5080
PciI A'CATG_T
Cuts at: 3979 3979
Size: 5080
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5080
PfoI T'CCnGG_A
Cuts at: 2265 2265
Size: 5080
PmeI GTTT'AAAC
Cuts at: 2384 2384
Size: 5080
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3149 3181 4662 4694 3149
Size: 32 1481 32 3535
Fragments arranged by size:
3535 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2151 2319 2151
Size: 168 4912
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5080
PsiI TTA'TAA
Cuts at: 2650 2650
Size: 5080
PstI C_TGCA'G
Cuts at: 830 2289 2432 830
Size: 1459 143 3478
Fragments arranged by size:
3478 1459 143
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5080
RsrII CG'GwC_CG
Cuts at: 2013 2013
Size: 5080
SacII CC_GC'GG
Cuts at: 2127 2317 2127
Size: 190 4890
SalI G'TCGA_C
Cuts at: 1936 2422 1936
Size: 486 4594
SbfI CC_TGCA'GG
Cuts at: 2432 2432
Size: 5080
ScaI AGT'ACT
Cuts at: 3328 5040 3328
Size: 1712 3368
SfcI C'TryA_G
Cuts at: 826 1045 2179 2285 2428 3563 4244 4435 826
Size: 219 1134 106 143 1135 681 191 1471
Fragments arranged by size:
1471 1135 1134 681 219 191 143 106
SfoI GGC'GCC
Cuts at: 2061 2212 2061
Size: 151 4929
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5080
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5080
SmaI CCC'GGG
Cuts at: 1872 2199 2407 1872
Size: 327 208 4545
Fragments arranged by size:
4545 327 208
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3184 4085 4347 820
Size: 197 926 1241 901 262 1553
Fragments arranged by size:
1553 1241 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2871 493
Size: 2378 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5080
SphI G_CATG'C
Cuts at: 1132 2438 1132
Size: 1306 3774
SspI AAT'ATT
Cuts at: 5 52 3004 5
Size: 47 2952 2081
Fragments arranged by size:
2952 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5080
StyI C'CwwG_G
Cuts at: 513 1065 2004 2519 513
Size: 552 939 515 3074
Fragments arranged by size:
3074 939 552 515
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3107 3124 3277 3462 3907 325
Size: 1255 1527 17 153 185 445 1498
Fragments arranged by size:
1527 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3326 5038 96
Size: 274 80 33 51 1443 1349 1712 138
Fragments arranged by size:
1712 1443 1349 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5040 5072 5040
Size: 32 5048
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5080
XbaI T'CTAG_A
Cuts at: 2416 2416
Size: 5080
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5080
XmaI C'CCGG_G
Cuts at: 1870 2197 2405 1870
Size: 327 208 4545
Fragments arranged by size:
4545 327 208
XmnI GAAnn'nnTTC
Cuts at: 2342 3209 2342
Size: 867 4213
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2272 2888 276
Size: 53 83 186 936 738 616 2468
Fragments arranged by size:
2468 936 738 616 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI RsrII SacII SalI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI Bpu10I BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FalI FseI FspAI NruI NsiI
PacI PmlI PspOMI PspXI PsrI PvuII SacI SanDI
SapI SexAI SfiI SrfI SwaI XcmI