Restriction map for
pFN21AE3014
(Circular) (Six-base) MAPSORT of: pFN21AE3014.seq Check: 6713 from: 1 to: 5017
pFN21AE3014 5017 bp
With 182 enzymes: SgfI *
March 28, 2012 12:59 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2827 278
Size: 53 83 186 936 1291 2468
Fragments arranged by size:
2468 1291 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2360 1937
Size: 423 4594
Acc65I G'GTAC_C
Cuts at: 2338 2338
Size: 5017
AclI AA'CG_TT
Cuts at: 1563 3144 3517 1563
Size: 1581 373 3063
Fragments arranged by size:
3063 1581 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2127 2133 2261 3080 4464 4732 1632
Size: 495 6 128 819 1384 268 1917
Fragments arranged by size:
1917 1384 819 495 268 128 6
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4820
AflIII A'CryG_T
Cuts at: 1203 3916 4893 1203
Size: 2713 977 1327
Fragments arranged by size:
2713 1327 977
AgeI A'CCGG_T
Cuts at: 3848 3848
Size: 5017
AhdI GACnn_n'nnGTC
Cuts at: 3746 3746
Size: 5017
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5017
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5017
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2042 2074 2042
Size: 32 4985
AlwNI CAG_nnn'CTG
Cuts at: 2137 2760 4332 2137
Size: 623 1572 2822
Fragments arranged by size:
2822 1572 623
ApaLI G'TGCA_C
Cuts at: 3074 4230 3074
Size: 1156 3861
ApoI r'AATT_y
Cuts at: 1493 2256 2326 2552 2704 4813 1493
Size: 763 70 226 152 2109 1697
Fragments arranged by size:
2109 1697 763 226 152 70
AseI AT'TA_AT
Cuts at: 160 1032 3571 160
Size: 872 2539 1606
Fragments arranged by size:
2539 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5017
AvaI C'yCGr_G
Cuts at: 1870 1943 2342 1870
Size: 73 399 4545
Fragments arranged by size:
4545 399 73
BamHI G'GATC_C
Cuts at: 2347 2714 2347
Size: 367 4650
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2338 3793 618
Size: 325 217 627 551 1455 1842
Fragments arranged by size:
1842 1455 627 551 325 217
BanII G_rGCy'C
Cuts at: 1391 2012 2336 1391
Size: 621 324 4072
Fragments arranged by size:
4072 621 324
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2153 928
Size: 971 254 3792
Fragments arranged by size:
3792 971 254
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3208 3242 1903
Size: 34 1271 34 3678
Fragments arranged by size:
3678 1271 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2909 4119 2909
Size: 1210 3807
BclI T'GATC_A
Cuts at: 1548 2193 1548
Size: 645 4372
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3628 136
Size: 107 122 71 631 605 1956 1525
Fragments arranged by size:
1956 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5012 5012
Size: 5017
BlpI GC'TnA_GC
Cuts at: 2434 2434
Size: 5017
Bme1580I G_kGCm'C
Cuts at: 1790 3078 4234 1790
Size: 1288 1156 2573
Fragments arranged by size:
2573 1288 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5017
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2748 3706 442
Size: 416 53 83 399 24 1331 958 1753
Fragments arranged by size:
1753 1331 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5017
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2207 3677 1239
Size: 968 1470 2579
Fragments arranged by size:
2579 1470 968
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3142 4007 4305 3142
Size: 865 298 3854
Fragments arranged by size:
3854 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3680 882
Size: 617 2181 2219
Fragments arranged by size:
2219 2181 617
BsaAI yAC'GTr
Cuts at: 493 1204 2808 493
Size: 711 1604 2702
Fragments arranged by size:
2702 1604 711
BsaBI GATnn'nnATC
Cuts at: 5011 5011
Size: 5017
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2824 3206 3841 275
Size: 53 83 186 936 1291 382 635 1451
Fragments arranged by size:
1451 1291 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3450 3848 4122 4269 4680 3450
Size: 398 274 147 411 3787
Fragments arranged by size:
3787 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2042 2072 1178
Size: 30 165 30 639 30 4123
Fragments arranged by size:
4123 639 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5017
BseYI C'CCAG_C
Cuts at: 4220 4716 4220
Size: 496 4521
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4444
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2467 3228 4256 1540
Size: 275 186 466 761 1028 2301
Fragments arranged by size:
2301 1028 761 466 275 186
BsiHKAI G_wGCw'C
Cuts at: 2012 3078 3163 4234 2012
Size: 1066 85 1071 2795
Fragments arranged by size:
2795 1071 1066 85
BsmI GAATG_Cn'
Cuts at: 2528 2621 2528
Size: 93 4924
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2012 2336 3078 3163 4234 1391
Size: 399 222 324 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 324 222 85
BspEI T'CCGG_A
Cuts at: 4680 4680
Size: 5017
BspHI T'CATG_A
Cuts at: 2906 4636 2906
Size: 1730 3287
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2136 2372 844
Size: 1292 236 3489
Fragments arranged by size:
3489 1292 236
BsrBI CCG'CTC
Cuts at: 1394 1823 2904 1394
Size: 429 1081 3507
Fragments arranged by size:
3507 1081 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3512 3686 65
Size: 1183 2264 174 1396
Fragments arranged by size:
2264 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3661 3848 1700
Size: 110 1851 187 2869
Fragments arranged by size:
2869 1851 187 110
BsrGI T'GTAC_A
Cuts at: 96 2283 96
Size: 2187 2830
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5017
BssSI C'ACGA_G
Cuts at: 3077 4089 3077
Size: 1012 4005
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2278 794
Size: 1484 3533
BstBI TT'CG_AA
Cuts at: 3821 3821
Size: 5017
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5017
BstYI r'GATC_y
Cuts at: 1972 2266 2347 2714 3099 3116 4568 4654 4666
Size: 294 81 367 385 17 1452 86 12
Cuts at: 4666 5012 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 385 367 346 294 86 81
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4465
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4278
Fragments arranged by size:
4278 683 56
BtsI GCAGTG_nn'
Cuts at: 2138 2209 2217 2533 3327 3347 2138
Size: 71 8 316 794 20 3808
Fragments arranged by size:
3808 794 316 71 20 8
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5017
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 4982
DraI TTT'AAA
Cuts at: 2321 2321
Size: 5017
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4024 809
Size: 507 2708 1802
Fragments arranged by size:
2708 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2464 3353 4982
Size: 54 1425 50 275 18 634 889 1629
Cuts at: 4982 8
Size: 43
Fragments arranged by size:
1629 1425 889 634 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2464 1537
Size: 275 652 4090
Fragments arranged by size:
4090 652 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2947 1353
Size: 1594 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3604 3978 4124 387
Size: 1064 138 2015 374 146 1280
Fragments arranged by size:
2015 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2010 2010
Size: 5017
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2127 2133 2207 2261 3080 3677 4464
Size: 393 495 6 74 54 819 597 787
Cuts at: 4464 4732 1239
Size: 268 1524
Fragments arranged by size:
1524 819 787 597 495 393 268 74
54 6
EcoO109I rG'GnC_Cy
Cuts at: 2022 2125 2022
Size: 103 4914
EcoRI G'AATT_C
Cuts at: 2326 2704 2326
Size: 378 4639
FspI TGC'GCA
Cuts at: 3523 3523
Size: 5017
HaeII r_GCGC'y
Cuts at: 1678 4164 1678
Size: 2486 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3658 3690 3732 3764 1373
Size: 32 135 32 2086 32 42 32 2626
Fragments arranged by size:
2626 2086 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2361 2607 669
Size: 988 281 423 246 3079
Fragments arranged by size:
3079 988 423 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5017
HpaI GTT'AAC
Cuts at: 2607 2607
Size: 5017
KpnI G_GTAC'C
Cuts at: 2342 2342
Size: 5017
MfeI C'AATT_G
Cuts at: 2616 2616
Size: 5017
MluI A'CGCG_T
Cuts at: 4893 4893
Size: 5017
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4131 4315 1305
Size: 2826 184 2007
Fragments arranged by size:
2826 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3195
Fragments arranged by size:
3195 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2975 3334 3493 518
Size: 648 1809 359 159 2042
Fragments arranged by size:
2042 1809 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2433 3110 4258 4503 1606
Size: 827 677 1148 245 2120
Fragments arranged by size:
2120 1148 827 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4465
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5017
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5017
NotI GC'GGCC_GC
Cuts at: 2464 2464
Size: 5017
NspI r_CATG'y
Cuts at: 1132 1229 2375 3920 1132
Size: 97 1146 1545 2229
Fragments arranged by size:
2229 1545 1146 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5017
PciI A'CATG_T
Cuts at: 3916 3916
Size: 5017
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5017
PmeI GTTT'AAAC
Cuts at: 2321 2321
Size: 5017
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2042 2074 3086 3118 4599 4631 2042
Size: 32 1012 32 1481 32 2428
Fragments arranged by size:
2428 1481 1012 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2022 2125 2022
Size: 103 4914
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5017
PsiI TTA'TAA
Cuts at: 2587 2587
Size: 5017
PstI C_TGCA'G
Cuts at: 830 2133 2369 830
Size: 1303 236 3478
Fragments arranged by size:
3478 1303 236
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5017
SacI G_AGCT'C
Cuts at: 2012 2012
Size: 5017
SalI G'TCGA_C
Cuts at: 1936 2359 1936
Size: 423 4594
SbfI CC_TGCA'GG
Cuts at: 2369 2369
Size: 5017
ScaI AGT'ACT
Cuts at: 3265 4977 3265
Size: 1712 3305
SfcI C'TryA_G
Cuts at: 826 1045 2129 2365 3500 4181 4372 826
Size: 219 1084 236 1135 681 191 1471
Fragments arranged by size:
1471 1135 1084 681 236 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5017
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5017
SmaI CCC'GGG
Cuts at: 1872 2344 1872
Size: 472 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3121 4022 4284 820
Size: 197 926 1178 901 262 1553
Fragments arranged by size:
1553 1178 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2808 493
Size: 2315 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5017
SphI G_CATG'C
Cuts at: 1132 2375 1132
Size: 1243 3774
SspI AAT'ATT
Cuts at: 5 52 2941 5
Size: 47 2889 2081
Fragments arranged by size:
2889 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5017
StyI C'CwwG_G
Cuts at: 513 1065 2456 513
Size: 552 1391 3074
Fragments arranged by size:
3074 1391 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3044 3061 3214 3399 3844 325
Size: 1255 1464 17 153 185 445 1498
Fragments arranged by size:
1498 1464 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2283 3263 4975
Size: 274 80 33 51 1443 306 980 1712
Cuts at: 4975 96
Size: 138
Fragments arranged by size:
1712 1443 980 306 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2117 2149 4977 5009 2117
Size: 32 2828 32 2125
Fragments arranged by size:
2828 2125 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5017
XbaI T'CTAG_A
Cuts at: 2353 2353
Size: 5017
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5017
XmaI C'CCGG_G
Cuts at: 1870 2342 1870
Size: 472 4545
XmnI GAAnn'nnTTC
Cuts at: 3146 3146
Size: 5017
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2825 276
Size: 53 83 186 936 1291 2468
Fragments arranged by size:
2468 1291 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI
PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI