(Circular) (Six-base) MAPSORT of: pFN21AE3006.seq Check: 470 from: 1 to: 4960 pFN21AE3006 4960 bp With 182 enzymes: SgfI * December 28, 2011 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2039 2770 278 Size: 53 83 186 936 503 731 2468 Fragments arranged by size: 2468 936 731 503 186 83 53 AccI GT'mk_AC Cuts at: 1937 2303 1937 Size: 366 4594 Acc65I G'GTAC_C Cuts at: 2281 2281 Size: 4960 AclI AA'CG_TT Cuts at: 1563 3087 3460 1563 Size: 1524 373 3063 Fragments arranged by size: 3063 1524 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3023 4407 4675 1632 Size: 1391 1384 268 1917 Fragments arranged by size: 1917 1391 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4763 AflIII A'CryG_T Cuts at: 1203 3859 4836 1203 Size: 2656 977 1327 Fragments arranged by size: 2656 1327 977 AgeI A'CCGG_T Cuts at: 3791 3791 Size: 4960 AhdI GACnn_n'nnGTC Cuts at: 3689 3689 Size: 4960 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 4960 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 4960 AlwNI CAG_nnn'CTG Cuts at: 2703 4275 2703 Size: 1572 3388 ApaLI G'TGCA_C Cuts at: 3017 4173 3017 Size: 1156 3804 ApoI r'AATT_y Cuts at: 1493 2120 2269 2495 2647 4756 1493 Size: 627 149 226 152 2109 1697 Fragments arranged by size: 2109 1697 627 226 152 149 AseI AT'TA_AT Cuts at: 160 1032 3514 160 Size: 872 2482 1606 Fragments arranged by size: 2482 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 4960 AvaI C'yCGr_G Cuts at: 1870 1943 2285 1870 Size: 73 342 4545 Fragments arranged by size: 4545 342 73 BamHI G'GATC_C Cuts at: 2290 2657 2290 Size: 367 4593 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2281 3736 618 Size: 325 217 627 494 1455 1842 Fragments arranged by size: 1842 1455 627 494 325 217 BanII G_rGCy'C Cuts at: 1391 2279 1391 Size: 888 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 3989 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3151 3185 1903 Size: 34 1214 34 3678 Fragments arranged by size: 3678 1214 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2852 4062 2852 Size: 1210 3750 BclI T'GATC_A Cuts at: 1548 1548 Size: 4960 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3571 136 Size: 107 122 71 631 605 1899 1525 Fragments arranged by size: 1899 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4955 4955 Size: 4960 BlpI GC'TnA_GC Cuts at: 2377 2377 Size: 4960 Bme1580I G_kGCm'C Cuts at: 1790 3021 4177 1790 Size: 1231 1156 2573 Fragments arranged by size: 2573 1231 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 4960 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2691 3649 442 Size: 416 53 83 399 24 1274 958 1753 Fragments arranged by size: 1753 1274 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 4960 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3620 1239 Size: 2381 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3085 3950 4248 3085 Size: 865 298 3797 Fragments arranged by size: 3797 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3623 882 Size: 617 2124 2219 Fragments arranged by size: 2219 2124 617 BsaAI yAC'GTr Cuts at: 493 1204 2751 493 Size: 711 1547 2702 Fragments arranged by size: 2702 1547 711 BsaBI GATnn'nnATC Cuts at: 4954 4954 Size: 4960 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2036 2767 3149 3784 Size: 53 83 186 936 503 731 382 635 Cuts at: 3784 275 Size: 1451 Fragments arranged by size: 1451 936 731 635 503 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3393 3791 4065 4212 4623 3393 Size: 398 274 147 411 3730 Fragments arranged by size: 3730 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4735 Fragments arranged by size: 4735 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 4960 BseYI C'CCAG_C Cuts at: 4163 4659 4163 Size: 496 4464 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2166 1163 Size: 573 430 3957 Fragments arranged by size: 3957 573 430 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2410 3171 4199 1540 Size: 275 186 409 761 1028 2301 Fragments arranged by size: 2301 1028 761 409 275 186 BsiHKAI G_wGCw'C Cuts at: 3021 3106 4177 3021 Size: 85 1071 3804 Fragments arranged by size: 3804 1071 85 BsmI GAATG_Cn' Cuts at: 2471 2564 2471 Size: 93 4867 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2279 3021 3106 4177 1391 Size: 399 489 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 489 399 85 BspEI T'CCGG_A Cuts at: 4623 4623 Size: 4960 BspHI T'CATG_A Cuts at: 2849 4579 2849 Size: 1730 3230 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2315 844 Size: 1471 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2847 1394 Size: 429 1024 3507 Fragments arranged by size: 3507 1024 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3455 3629 65 Size: 1183 2207 174 1396 Fragments arranged by size: 2207 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3604 3791 1700 Size: 110 1794 187 2869 Fragments arranged by size: 2869 1794 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 4960 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 4960 BssSI C'ACGA_G Cuts at: 3020 4032 3020 Size: 1012 3948 BstAPI GCAn_nnn'nTGC Cuts at: 794 2144 2189 794 Size: 1350 45 3565 Fragments arranged by size: 3565 1350 45 BstBI TT'CG_AA Cuts at: 3764 3764 Size: 4960 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 4960 BstYI r'GATC_y Cuts at: 1972 2290 2657 3042 3059 4511 4597 4609 4955 Size: 318 367 385 17 1452 86 12 346 Cuts at: 4955 1972 Size: 1977 Fragments arranged by size: 1977 1452 385 367 346 318 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2045 513 Size: 552 980 3428 Fragments arranged by size: 3428 980 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4221 Fragments arranged by size: 4221 683 56 BtsI GCAGTG_nn' Cuts at: 2476 3270 3290 2476 Size: 794 20 4146 Fragments arranged by size: 4146 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 4960 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 4925 DraI TTT'AAA Cuts at: 2125 2264 2125 Size: 139 4821 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3967 809 Size: 507 2651 1802 Fragments arranged by size: 2651 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2407 3296 4925 Size: 54 1425 50 275 18 577 889 1629 Cuts at: 4925 8 Size: 43 Fragments arranged by size: 1629 1425 889 577 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2407 1537 Size: 275 595 4090 Fragments arranged by size: 4090 595 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2890 1353 Size: 1537 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3547 3921 4067 387 Size: 1064 138 1958 374 146 1280 Fragments arranged by size: 1958 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3023 3620 4407 4675 1239 Size: 393 1391 597 787 268 1524 Fragments arranged by size: 1524 1391 787 597 393 268 EcoRI G'AATT_C Cuts at: 2269 2647 2269 Size: 378 4582 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2035 2103 2035 Size: 68 4892 FspI TGC'GCA Cuts at: 3466 3466 Size: 4960 HaeII r_GCGC'y Cuts at: 1678 4107 1678 Size: 2429 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3601 3633 3675 3707 1373 Size: 32 135 32 2029 32 42 32 2626 Fragments arranged by size: 2626 2029 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2304 2550 669 Size: 988 281 366 246 3079 Fragments arranged by size: 3079 988 366 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 4960 HpaI GTT'AAC Cuts at: 2550 2550 Size: 4960 KpnI G_GTAC'C Cuts at: 2285 2285 Size: 4960 MfeI C'AATT_G Cuts at: 2559 2559 Size: 4960 MluI A'CGCG_T Cuts at: 4836 4836 Size: 4960 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2019 2230 4074 4258 1305 Size: 714 211 1844 184 2007 Fragments arranged by size: 2007 1844 714 211 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3138 Fragments arranged by size: 3138 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2918 3277 3436 518 Size: 648 1752 359 159 2042 Fragments arranged by size: 2042 1752 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2376 3053 4201 4446 1606 Size: 770 677 1148 245 2120 Fragments arranged by size: 2120 1148 770 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4408 NdeI CA'TA_TG Cuts at: 387 387 Size: 4960 NheI G'CTAG_C Cuts at: 1052 1052 Size: 4960 NotI GC'GGCC_GC Cuts at: 2407 2407 Size: 4960 NspI r_CATG'y Cuts at: 1132 1229 2318 3863 1132 Size: 97 1089 1545 2229 Fragments arranged by size: 2229 1545 1089 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 4960 PciI A'CATG_T Cuts at: 3859 3859 Size: 4960 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 4960 PmeI GTTT'AAAC Cuts at: 2264 2264 Size: 4960 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3029 3061 4542 4574 3029 Size: 32 1481 32 3415 Fragments arranged by size: 3415 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 4960 PsiI TTA'TAA Cuts at: 2530 2530 Size: 4960 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2055 2087 2055 Size: 32 4928 PstI C_TGCA'G Cuts at: 830 2312 830 Size: 1482 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 4960 SalI G'TCGA_C Cuts at: 1936 2302 1936 Size: 366 4594 SbfI CC_TGCA'GG Cuts at: 2312 2312 Size: 4960 ScaI AGT'ACT Cuts at: 2064 3208 4920 2064 Size: 1144 1712 2104 Fragments arranged by size: 2104 1712 1144 SfcI C'TryA_G Cuts at: 826 1045 2308 3443 4124 4315 826 Size: 219 1263 1135 681 191 1471 Fragments arranged by size: 1471 1263 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 4960 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 4960 SmaI CCC'GGG Cuts at: 1872 2287 1872 Size: 415 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3064 3965 4227 820 Size: 197 926 1121 901 262 1553 Fragments arranged by size: 1553 1121 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2751 493 Size: 2258 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 4960 SphI G_CATG'C Cuts at: 1132 2318 1132 Size: 1186 3774 SspI AAT'ATT Cuts at: 5 52 2884 5 Size: 47 2832 2081 Fragments arranged by size: 2832 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 4960 StyI C'CwwG_G Cuts at: 513 1065 2399 513 Size: 552 1334 3074 Fragments arranged by size: 3074 1334 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2987 3004 3157 3342 3787 325 Size: 1255 1407 17 153 185 445 1498 Fragments arranged by size: 1498 1407 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2062 2081 3206 Size: 274 80 33 51 1443 85 19 1125 Cuts at: 3206 4918 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1125 274 138 85 80 51 33 19 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4920 4952 4920 Size: 32 4928 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 4960 XbaI T'CTAG_A Cuts at: 2296 2296 Size: 4960 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 4960 XmaI C'CCGG_G Cuts at: 1870 2285 1870 Size: 415 4545 XmnI GAAnn'nnTTC Cuts at: 3089 3089 Size: 4960 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2037 2768 276 Size: 53 83 186 936 503 731 2468 Fragments arranged by size: 2468 936 731 503 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PsrI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI