Restriction map for
pFN21AE2800
(Circular) (Six-base) MAPSORT of: pFN21AE2800.seq Check: 5857 from: 1 to: 6196
pFN21AE2800 6196 bp
With 182 enzymes: SgfI *
August 16, 2021 15:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4006 278
Size: 53 83 186 936 2470 2468
Fragments arranged by size:
2470 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3539 1937
Size: 1602 4594
Acc65I G'GTAC_C
Cuts at: 3517 3517
Size: 6196
AclI AA'CG_TT
Cuts at: 1563 4323 4696 1563
Size: 2760 373 3063
Fragments arranged by size:
3063 2760 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3175 3477 4259 5643 5911 1632
Size: 1543 302 782 1384 268 1917
Fragments arranged by size:
1917 1543 1384 782 302 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5999
AflIII A'CryG_T
Cuts at: 1203 3204 5095 6072 1203
Size: 2001 1891 977 1327
Fragments arranged by size:
2001 1891 1327 977
AgeI A'CCGG_T
Cuts at: 5027 5027
Size: 6196
AhdI GACnn_n'nnGTC
Cuts at: 2764 4925 2764
Size: 2161 4035
AleI CACnn'nnGTG
Cuts at: 1166 2501 1166
Size: 1335 4861
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6196
AlwNI CAG_nnn'CTG
Cuts at: 2365 2414 2959 3313 3939 5511 2365
Size: 49 545 354 626 1572 3050
Fragments arranged by size:
3050 1572 626 545 354 49
ApaI G_GGCC'C
Cuts at: 2914 2914
Size: 6196
ApaLI G'TGCA_C
Cuts at: 4253 5409 4253
Size: 1156 5040
ApoI r'AATT_y
Cuts at: 1493 2220 2387 3505 3731 3883 5992 1493
Size: 727 167 1118 226 152 2109 1697
Fragments arranged by size:
2109 1697 1118 727 226 167 152
AseI AT'TA_AT
Cuts at: 160 1032 4750 160
Size: 872 3718 1606
Fragments arranged by size:
3718 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6196
AvaI C'yCGr_G
Cuts at: 1870 1943 2073 3521 1870
Size: 73 130 1448 4545
Fragments arranged by size:
4545 1448 130 73
AvrII C'CTAG_G
Cuts at: 2527 2527
Size: 6196
BamHI G'GATC_C
Cuts at: 3526 3893 3526
Size: 367 5829
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3517 4972 618
Size: 325 217 627 1730 1455 1842
Fragments arranged by size:
1842 1730 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2157 2322 2522 2914 3515 1391
Size: 766 165 200 392 601 4072
Fragments arranged by size:
4072 766 601 392 200 165
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2203 3181 928
Size: 971 304 978 3943
Fragments arranged by size:
3943 978 971 304
BbvCI CC'TCA_GC
Cuts at: 3248 3248
Size: 6196
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2092 2126 2541 2575 4387 4421 1903
Size: 34 155 34 415 34 1812 34 3678
Fragments arranged by size:
3678 1812 415 155 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4088 5298 4088
Size: 1210 4986
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6196
BfrBI ATG'CAT
Cuts at: 3176 3176
Size: 6196
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4807 136
Size: 107 122 71 631 605 3135 1525
Fragments arranged by size:
3135 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6191 6191
Size: 6196
BlpI GC'TnA_GC
Cuts at: 2321 3613 2321
Size: 1292 4904
Bme1580I G_kGCm'C
Cuts at: 1790 2016 2914 4257 5413 1790
Size: 226 898 1343 1156 2573
Fragments arranged by size:
2573 1343 1156 898 226
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6196
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3041 3927 4885
Size: 416 53 83 399 24 1624 886 958
Cuts at: 4885 442
Size: 1753
Fragments arranged by size:
1753 1624 958 886 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6196
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4856 1239
Size: 3617 2579
Bpu10I CC'TnA_GC
Cuts at: 3248 3248
Size: 6196
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3465 4321 5186 5484 3465
Size: 856 865 298 4177
Fragments arranged by size:
4177 865 856 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4859 882
Size: 617 3360 2219
Fragments arranged by size:
3360 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3987 493
Size: 711 2783 2702
Fragments arranged by size:
2783 2702 711
BsaBI GATnn'nnATC
Cuts at: 6190 6190
Size: 6196
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4003 4385 5020 275
Size: 53 83 186 936 2470 382 635 1451
Fragments arranged by size:
2470 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2809 4629 5027 5301 5448 5859 2809
Size: 1820 398 274 147 411 3146
Fragments arranged by size:
3146 1820 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5971
Fragments arranged by size:
5971 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2804 2866 1186
Size: 1618 62 4516
Fragments arranged by size:
4516 1618 62
BseYI C'CCAG_C
Cuts at: 2176 2728 2751 5399 5895 2176
Size: 552 23 2648 496 2477
Fragments arranged by size:
2648 2477 552 496 23
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3447 1163
Size: 573 1711 3912
Fragments arranged by size:
3912 1711 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2106 3646 4407 5435 1540
Size: 275 186 105 1540 761 1028 2301
Fragments arranged by size:
2301 1540 1028 761 275 186 105
BsiHKAI G_wGCw'C
Cuts at: 2061 4257 4342 5413 2061
Size: 2196 85 1071 2844
Fragments arranged by size:
2844 2196 1071 85
BsmI GAATG_Cn'
Cuts at: 2093 3707 3800 2093
Size: 1614 93 4489
Fragments arranged by size:
4489 1614 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2834 2834
Size: 6196
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2016 2061 2157 2322 2522 2914 3515
Size: 399 226 45 96 165 200 392 601
Cuts at: 3515 4257 4342 5413 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 601 399 392 226 200
165 96 85 45
BspEI T'CCGG_A
Cuts at: 5859 5859
Size: 6196
BspHI T'CATG_A
Cuts at: 4085 5815 4085
Size: 1730 4466
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2181 2681 3551 844
Size: 1337 500 870 3489
Fragments arranged by size:
3489 1337 870 500
BsrBI CCG'CTC
Cuts at: 1394 1823 2072 4083 1394
Size: 429 249 2011 3507
Fragments arranged by size:
3507 2011 429 249
BsrDI GCAATG_nn'
Cuts at: 65 1248 3291 4691 4865 65
Size: 1183 2043 1400 174 1396
Fragments arranged by size:
2043 1400 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4840 5027 1700
Size: 110 3030 187 2869
Fragments arranged by size:
3030 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6196
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6196
BssSI C'ACGA_G
Cuts at: 4256 5268 4256
Size: 1012 5184
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3313 794
Size: 2519 3677
BstBI TT'CG_AA
Cuts at: 5000 5000
Size: 6196
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6196
BstXI CCAn_nnnn'nTGG
Cuts at: 2503 2747 2503
Size: 244 5952
BstYI r'GATC_y
Cuts at: 1972 3463 3526 3893 4278 4295 5747 5833 5845
Size: 1491 63 367 385 17 1452 86 12
Cuts at: 5845 6191 1972
Size: 346 1977
Fragments arranged by size:
1977 1491 1452 385 367 346 86 63
17 12
Bsu36I CC'TnA_GG
Cuts at: 2141 2141
Size: 6196
BtgI C'CryG_G
Cuts at: 513 1065 2004 2799 513
Size: 552 939 795 3910
Fragments arranged by size:
3910 939 795 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2386 3385 487
Size: 683 56 1160 999 3298
Fragments arranged by size:
3298 1160 999 683 56
BtsI GCAGTG_nn'
Cuts at: 3138 3302 3712 4506 4526 3138
Size: 164 410 794 20 4808
Fragments arranged by size:
4808 794 410 164 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6196
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2401 2436 529
Size: 35 1837 35 4289
Fragments arranged by size:
4289 1837 35 35
DraI TTT'AAA
Cuts at: 3500 3500
Size: 6196
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5203 809
Size: 507 3887 1802
Fragments arranged by size:
3887 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2049 2370 3417
Size: 54 1425 50 275 18 219 321 1047
Cuts at: 3417 3643 4532 6161 8
Size: 226 889 1629 43
Fragments arranged by size:
1629 1425 1047 889 321 275 226 219
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3643 1537
Size: 275 1831 4090
Fragments arranged by size:
4090 1831 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2636 2654 2892 4126 1353
Size: 1283 18 238 1234 3423
Fragments arranged by size:
3423 1283 1234 238 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4783 5157 5303 387
Size: 1064 138 3194 374 146 1280
Fragments arranged by size:
3194 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3175 3477 4259 4856 5643 5911 1239
Size: 393 1543 302 782 597 787 268 1524
Fragments arranged by size:
1543 1524 787 782 597 393 302 268
EcoO109I rG'GnC_Cy
Cuts at: 2911 2956 2911
Size: 45 6151
EcoRI G'AATT_C
Cuts at: 3505 3883 3505
Size: 378 5818
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2514 2665 2514
Size: 151 6045
FspI TGC'GCA
Cuts at: 4702 4702
Size: 6196
HaeII r_GCGC'y
Cuts at: 1678 5343 1678
Size: 3665 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2750 2782 2965 2997 3088
Size: 32 135 32 1178 32 183 32 91
Cuts at: 3088 3120 3455 3487 4837 4869 4911 4943 1373
Size: 32 335 32 1350 32 42 32 2626
Fragments arranged by size:
2626 1350 1178 335 183 135 91 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3081 3540 3786 669
Size: 988 281 1143 459 246 3079
Fragments arranged by size:
3079 1143 988 459 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6196
HpaI GTT'AAC
Cuts at: 3786 3786
Size: 6196
KpnI G_GTAC'C
Cuts at: 3521 3521
Size: 6196
MfeI C'AATT_G
Cuts at: 3795 3795
Size: 6196
MluI A'CGCG_T
Cuts at: 6072 6072
Size: 6196
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5310 5494 1305
Size: 4005 184 2007
Fragments arranged by size:
4005 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2372 3419 10
Size: 54 1425 343 540 1047 2787
Fragments arranged by size:
2787 1425 1047 540 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2501 3164 3203 3404 4154 4513
Size: 648 843 492 663 39 201 750 359
Cuts at: 4513 4672 518
Size: 159 2042
Fragments arranged by size:
2042 843 750 663 648 492 359 201
159 39
MspA1I CmG'CkG
Cuts at: 1606 3612 4289 5437 5682 1606
Size: 2006 677 1148 245 2120
Fragments arranged by size:
2120 2006 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4705
Fragments arranged by size:
4705 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6196
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6196
NotI GC'GGCC_GC
Cuts at: 3643 3643
Size: 6196
NruI TCG'CGA
Cuts at: 2085 2085
Size: 6196
NsiI A_TGCA'T
Cuts at: 3178 3178
Size: 6196
NspI r_CATG'y
Cuts at: 1132 1229 2464 3208 3554 5099 1132
Size: 97 1235 744 346 1545 2229
Fragments arranged by size:
2229 1545 1235 744 346 97
PasI CC'CwG_GG
Cuts at: 1348 2359 1348
Size: 1011 5185
PciI A'CATG_T
Cuts at: 3204 5095 3204
Size: 1891 4305
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6196
PmeI GTTT'AAAC
Cuts at: 3500 3500
Size: 6196
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4265 4297 5778 5810 4265
Size: 32 1481 32 4651
Fragments arranged by size:
4651 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6196
PsiI TTA'TAA
Cuts at: 2295 3766 2295
Size: 1471 4725
PspOMI G'GGCC_C
Cuts at: 2910 2910
Size: 6196
PstI C_TGCA'G
Cuts at: 830 2316 3133 3309 3548 830
Size: 1486 817 176 239 3478
Fragments arranged by size:
3478 1486 817 239 176
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6196
RsrII CG'GwC_CG
Cuts at: 2067 2067
Size: 6196
SalI G'TCGA_C
Cuts at: 1936 3538 1936
Size: 1602 4594
SapI GCTCTTCn'nnn_
Cuts at: 2636 2636
Size: 6196
SbfI CC_TGCA'GG
Cuts at: 3548 3548
Size: 6196
ScaI AGT'ACT
Cuts at: 2847 4444 6156 2847
Size: 1597 1712 2887
Fragments arranged by size:
2887 1712 1597
SexAI A'CCwGG_T
Cuts at: 2247 2247
Size: 6196
SfcI C'TryA_G
Cuts at: 826 1045 2312 2440 3129 3305 3544 4679 5360
Size: 219 1267 128 689 176 239 1135 681
Cuts at: 5360 5551 826
Size: 191 1471
Fragments arranged by size:
1471 1267 1135 689 681 239 219 191
176 128
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6196
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6196
SmaI CCC'GGG
Cuts at: 1872 3523 1872
Size: 1651 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3480 4300 5201 5463 820
Size: 197 926 1537 820 901 262 1553
Fragments arranged by size:
1553 1537 926 901 820 262 197
SnaBI TAC'GTA
Cuts at: 493 3987 493
Size: 3494 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6196
SphI G_CATG'C
Cuts at: 1132 3554 1132
Size: 2422 3774
SspI AAT'ATT
Cuts at: 5 52 4120 5
Size: 47 4068 2081
Fragments arranged by size:
4068 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6196
StyI C'CwwG_G
Cuts at: 513 1065 2004 2527 3346 3635 513
Size: 552 939 523 819 289 3074
Fragments arranged by size:
3074 939 819 552 523 289
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2213 3023 3154 4223 4240 4393 4578
Size: 1255 633 810 131 1069 17 153 185
Cuts at: 4578 5023 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1069 810 633 445 185 153
131 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2845 4442 6154
Size: 274 80 33 51 1443 868 1597 1712
Cuts at: 6154 96
Size: 138
Fragments arranged by size:
1712 1597 1443 868 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6156 6188 6156
Size: 32 6164
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6196
XbaI T'CTAG_A
Cuts at: 2383 3532 2383
Size: 1149 5047
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2684 2684
Size: 6196
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6196
XmaI C'CCGG_G
Cuts at: 1870 3521 1870
Size: 1651 4545
XmnI GAAnn'nnTTC
Cuts at: 2858 4325 2858
Size: 1467 4729
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4004 276
Size: 53 83 186 936 2470 2468
Fragments arranged by size:
2470 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NruI NsiI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI
RsrII SalI SapI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI BaeI BbeI BplI BsiWI
BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI
NaeI NarI NgoMIV PacI PfoI PmlI PpuMI PspXI
PsrI PvuII SacI SacII SanDI SfiI SfoI SrfI
SwaI