Restriction map for
pFN21AE2797
(Circular) (Six-base) MAPSORT of: pFN21AE2797.seq Check: 5286 from: 1 to: 7078
pFN21AE2797 7078 bp
With 182 enzymes: SgfI *
August 16, 2021 15:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2727 4888 278
Size: 53 83 186 936 1191 2161 2468
Fragments arranged by size:
2468 2161 1191 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3228 4421 1937
Size: 1291 1193 4594
Fragments arranged by size:
4594 1291 1193
Acc65I G'GTAC_C
Cuts at: 4399 4399
Size: 7078
AclI AA'CG_TT
Cuts at: 1563 5205 5578 1563
Size: 3642 373 3063
Fragments arranged by size:
3642 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2381 2607 3095 3788 3862 5141 6525 6793
Size: 749 226 488 693 74 1279 1384 268
Cuts at: 6793 1632
Size: 1917
Fragments arranged by size:
1917 1384 1279 749 693 488 268 226
74
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6881
AflIII A'CryG_T
Cuts at: 1203 3321 5977 6954 1203
Size: 2118 2656 977 1327
Fragments arranged by size:
2656 2118 1327 977
AgeI A'CCGG_T
Cuts at: 5909 5909
Size: 7078
AhdI GACnn_n'nnGTC
Cuts at: 5807 5807
Size: 7078
AleI CACnn'nnGTG
Cuts at: 1166 3185 1166
Size: 2019 5059
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7078
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3551 3583 3551
Size: 32 7046
AlwNI CAG_nnn'CTG
Cuts at: 2845 4821 6393 2845
Size: 1976 1572 3530
Fragments arranged by size:
3530 1976 1572
ApaLI G'TGCA_C
Cuts at: 2124 2316 5135 6291 2124
Size: 192 2819 1156 2911
Fragments arranged by size:
2911 2819 1156 192
ApoI r'AATT_y
Cuts at: 1493 4358 4387 4613 4765 6874 1493
Size: 2865 29 226 152 2109 1697
Fragments arranged by size:
2865 2109 1697 226 152 29
AseI AT'TA_AT
Cuts at: 160 1032 5632 160
Size: 872 4600 1606
Fragments arranged by size:
4600 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7078
AvaI C'yCGr_G
Cuts at: 1870 1943 2828 3393 4261 4403 1870
Size: 73 885 565 868 142 4545
Fragments arranged by size:
4545 885 868 565 142 73
AvrII C'CTAG_G
Cuts at: 3457 3457
Size: 7078
BamHI G'GATC_C
Cuts at: 2568 4408 4775 2568
Size: 1840 367 4871
Fragments arranged by size:
4871 1840 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2029 2448 2717 4080 4399
Size: 325 217 627 242 419 269 1363 319
Cuts at: 4399 5854 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1363 627 419 325 319 269
242 217
BanII G_rGCy'C
Cuts at: 1391 4397 1391
Size: 3006 4072
BbeI G_GCGC'C
Cuts at: 2452 2721 4084 2452
Size: 269 1363 5446
Fragments arranged by size:
5446 1363 269
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2185 2516 3607 4238 4263 928
Size: 971 286 331 1091 631 25 3743
Fragments arranged by size:
3743 1091 971 631 331 286 25
BbvCI CC'TCA_GC
Cuts at: 4136 4136
Size: 7078
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5269 5303 1903
Size: 34 3332 34 3678
Fragments arranged by size:
3678 3332 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3963 4970 6180 3963
Size: 1007 1210 4861
Fragments arranged by size:
4861 1210 1007
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7078
BfrBI ATG'CAT
Cuts at: 4340 4340
Size: 7078
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2744 5689 136
Size: 107 122 71 631 605 1072 2945 1525
Fragments arranged by size:
2945 1525 1072 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7073 7073
Size: 7078
BlpI GC'TnA_GC
Cuts at: 4495 4495
Size: 7078
Bme1580I G_kGCm'C
Cuts at: 1790 2128 2320 5139 6295 1790
Size: 338 192 2819 1156 2573
Fragments arranged by size:
2819 2573 1156 338 192
BmgBI CAC'GTC
Cuts at: 1318 4240 1318
Size: 2922 4156
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2055 2257 2307
Size: 416 53 83 399 24 638 202 50
Cuts at: 2307 3004 3151 4809 5767 442
Size: 697 147 1658 958 1753
Fragments arranged by size:
1753 1658 958 697 638 416 399 202
147 83 53 50 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7078
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4072 4072
Size: 7078
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2159 3023 3290 5738 1239
Size: 920 864 267 2448 2579
Fragments arranged by size:
2579 2448 920 864 267
Bpu10I CC'TnA_GC
Cuts at: 4136 4136
Size: 7078
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2256 5203 6068 6366 2256
Size: 2947 865 298 2968
Fragments arranged by size:
2968 2947 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2282 2999 3523 5741 882
Size: 617 783 717 524 2218 2219
Fragments arranged by size:
2219 2218 783 717 617 524
BsaAI yAC'GTr
Cuts at: 493 1204 4869 493
Size: 711 3665 2702
Fragments arranged by size:
3665 2702 711
BsaBI GATnn'nnATC
Cuts at: 7072 7072
Size: 7078
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2449 2718 2724 2795
Size: 53 83 186 936 916 269 6 71
Cuts at: 2795 4081 4885 5267 5902 275
Size: 1286 804 382 635 1451
Fragments arranged by size:
1451 1286 936 916 804 635 382 269
186 83 71 53 6
BsaWI w'CCGG_w
Cuts at: 3114 4149 5511 5909 6183 6330 6741 3114
Size: 1035 1362 398 274 147 411 3451
Fragments arranged by size:
3451 1362 1035 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2170 2200 2240 2270 3848
Size: 30 165 30 767 30 40 30 1578
Cuts at: 3848 3878 1178
Size: 30 4378
Fragments arranged by size:
4378 1578 767 165 40 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2175 2259 2356 2417 2648 3413 3416 3998
Size: 989 84 97 61 231 765 3 582
Cuts at: 3998 4001 4153 1186
Size: 3 152 4111
Fragments arranged by size:
4111 989 765 582 231 152 97 84
61 3 3
BseYI C'CCAG_C
Cuts at: 3678 6281 6777 3678
Size: 2603 496 3979
Fragments arranged by size:
3979 2603 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2858 3263 1163
Size: 573 1122 405 4978
Fragments arranged by size:
4978 1122 573 405
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4528 5289 6317 1540
Size: 275 186 2527 761 1028 2301
Fragments arranged by size:
2527 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2128 2320 2856 3477 5139 5224 6295 2128
Size: 192 536 621 1662 85 1071 2911
Fragments arranged by size:
2911 1662 1071 621 536 192 85
BsiWI C'GTAC_G
Cuts at: 3147 3147
Size: 7078
BsmI GAATG_Cn'
Cuts at: 4589 4682 4589
Size: 93 6985
BsmBI CGTCTCn'nnnn_
Cuts at: 2786 3382 4060 4246 2786
Size: 596 678 186 5618
Fragments arranged by size:
5618 678 596 186
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2128 2320 2856 3477 4397 5139 5224
Size: 399 338 192 536 621 920 742 85
Cuts at: 5224 6295 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 920 742 621 536 399 338
192 85
BspEI T'CCGG_A
Cuts at: 3114 4149 6741 3114
Size: 1035 2592 3451
Fragments arranged by size:
3451 2592 1035
BspHI T'CATG_A
Cuts at: 3735 4967 6697 3735
Size: 1232 1730 4116
Fragments arranged by size:
4116 1730 1232
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2522 2598 4433 844
Size: 1678 76 1835 3489
Fragments arranged by size:
3489 1835 1678 76
BsrBI CCG'CTC
Cuts at: 1394 1823 2677 4965 1394
Size: 429 854 2288 3507
Fragments arranged by size:
3507 2288 854 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3510 4023 5573 5747 65
Size: 1183 2262 513 1550 174 1396
Fragments arranged by size:
2262 1550 1396 1183 513 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2095 2714 3249 5722 5909 1700
Size: 110 285 619 535 2473 187 2869
Fragments arranged by size:
2869 2473 619 535 285 187 110
BsrGI T'GTAC_A
Cuts at: 96 3726 96
Size: 3630 3448
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7078
BssSI C'ACGA_G
Cuts at: 2855 2886 3656 5138 6150 2855
Size: 31 770 1482 1012 3783
Fragments arranged by size:
3783 1482 1012 770 31
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3259 3286 794
Size: 2465 27 4586
Fragments arranged by size:
4586 2465 27
BstBI TT'CG_AA
Cuts at: 5882 5882
Size: 7078
BstEII G'GTnAC_C
Cuts at: 1184 2914 1184
Size: 1730 5348
BstXI CCAn_nnnn'nTGG
Cuts at: 2026 2206 3673 3919 2026
Size: 180 1467 246 5185
Fragments arranged by size:
5185 1467 246 180
BstYI r'GATC_y
Cuts at: 1972 2568 3069 3538 4408 4775 5160 5177 6629
Size: 596 501 469 870 367 385 17 1452
Cuts at: 6629 6715 6727 7073 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 870 596 501 469 385 367
346 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2205 2919 3198 3666 3717 513
Size: 552 1140 714 279 468 51 3874
Fragments arranged by size:
3874 1140 714 552 468 279 51
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2650 2677 2692 487
Size: 683 56 1424 27 15 4873
Fragments arranged by size:
4873 1424 683 56 27 15
BtsI GCAGTG_nn'
Cuts at: 2231 3894 4594 5388 5408 2231
Size: 1663 700 794 20 3901
Fragments arranged by size:
3901 1663 794 700 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7078
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7043
DraI TTT'AAA
Cuts at: 4382 4382
Size: 7078
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6085 809
Size: 507 4769 1802
Fragments arranged by size:
4769 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2325 2922 3045
Size: 54 1425 50 275 18 495 597 123
Cuts at: 3045 3663 4173 4525 5414 7043 8
Size: 618 510 352 889 1629 43
Fragments arranged by size:
1629 1425 889 618 597 510 495 352
275 123 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4525 1537
Size: 275 2713 4090
Fragments arranged by size:
4090 2713 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2084 2114 2328 2583 3905 5008 1353
Size: 731 30 214 255 1322 1103 3423
Fragments arranged by size:
3423 1322 1103 731 255 214 30
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2114 3080 5665 6039 6185 387
Size: 1064 138 525 966 2585 374 146 1280
Fragments arranged by size:
2585 1280 1064 966 525 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2159 2381 2607 3023 3095 3290 3788
Size: 393 527 222 226 416 72 195 498
Cuts at: 3788 3862 5141 5738 6525 6793 1239
Size: 74 1279 597 787 268 1524
Fragments arranged by size:
1524 1279 787 597 527 498 416 393
268 226 222 195 74 72
EcoNI CCTnn'n_nnAGG
Cuts at: 2522 2522
Size: 7078
EcoO109I rG'GnC_Cy
Cuts at: 2745 4265 2745
Size: 1520 5558
EcoRI G'AATT_C
Cuts at: 4358 4387 4765 4358
Size: 29 378 6671
Fragments arranged by size:
6671 378 29
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2389 2764 3614 2389
Size: 375 850 5853
Fragments arranged by size:
5853 850 375
FspI TGC'GCA
Cuts at: 5584 5584
Size: 7078
HaeII r_GCGC'y
Cuts at: 1678 2452 2660 2721 4084 6225 1678
Size: 774 208 61 1363 2141 2531
Fragments arranged by size:
2531 2141 1363 774 208 61
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2017 2049 2170 2202 2240
Size: 32 135 32 445 32 121 32 38
Cuts at: 2240 2272 2279 2311 2858 2890 3530 3562 3848
Size: 32 7 32 547 32 640 32 286
Cuts at: 3848 3880 5719 5751 5793 5825 1373
Size: 32 1839 32 42 32 2626
Fragments arranged by size:
2626 1839 640 547 445 286 135 121
42 38 32 32 32 32 32 32
32 32 32 32 32 7
HincII GTy'rAC
Cuts at: 669 1657 1938 2728 4422 4668 669
Size: 988 281 790 1694 246 3079
Fragments arranged by size:
3079 1694 988 790 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7078
HpaI GTT'AAC
Cuts at: 4668 4668
Size: 7078
KasI G'GCGC_C
Cuts at: 2448 2717 4080 2448
Size: 269 1363 5446
Fragments arranged by size:
5446 1363 269
KpnI G_GTAC'C
Cuts at: 4403 4403
Size: 7078
MfeI C'AATT_G
Cuts at: 4677 4677
Size: 7078
MluI A'CGCG_T
Cuts at: 6954 6954
Size: 7078
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2238 2523 4213 6192 6376 1305
Size: 933 285 1690 1979 184 2007
Fragments arranged by size:
2007 1979 1690 933 285 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2327 3665 10
Size: 54 1425 343 495 1338 3423
Fragments arranged by size:
3423 1425 1338 495 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3185 3497 3722 5036 5395 5554 518
Size: 648 2019 312 225 1314 359 159 2042
Fragments arranged by size:
2042 2019 1314 648 359 312 225 159
MspA1I CmG'CkG
Cuts at: 1606 2323 2842 3037 3043 3277 4494 5171 6319
Size: 717 519 195 6 234 1217 677 1148
Cuts at: 6319 6564 1606
Size: 245 2120
Fragments arranged by size:
2120 1217 1148 717 677 519 245 234
195 6
NaeI GCC'GGC
Cuts at: 2097 2716 2097
Size: 619 6459
NarI GG'CG_CC
Cuts at: 2449 2718 4081 2449
Size: 269 1363 5446
Fragments arranged by size:
5446 1363 269
NcoI C'CATG_G
Cuts at: 513 1065 2205 3666 3717 513
Size: 552 1140 1461 51 3874
Fragments arranged by size:
3874 1461 1140 552 51
NdeI CA'TA_TG
Cuts at: 387 3294 387
Size: 2907 4171
NgoMIV G'CCGG_C
Cuts at: 2095 2714 2095
Size: 619 6459
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7078
NotI GC'GGCC_GC
Cuts at: 4525 4525
Size: 7078
NsiI A_TGCA'T
Cuts at: 4342 4342
Size: 7078
NspI r_CATG'y
Cuts at: 1132 1229 2817 2965 3325 3691 3727 4021 4436
Size: 97 1588 148 360 366 36 294 415
Cuts at: 4436 5981 1132
Size: 1545 2229
Fragments arranged by size:
2229 1588 1545 415 366 360 294 148
97 36
PasI CC'CwG_GG
Cuts at: 1348 3673 1348
Size: 2325 4753
PciI A'CATG_T
Cuts at: 3321 5977 3321
Size: 2656 4422
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3718 1108
Size: 2610 4468
PfoI T'CCnGG_A
Cuts at: 2136 3125 4310 4362 2136
Size: 989 1185 52 4852
Fragments arranged by size:
4852 1185 989 52
PmeI GTTT'AAAC
Cuts at: 4382 4382
Size: 7078
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5147 5179 6660 6692 5147
Size: 32 1481 32 5533
Fragments arranged by size:
5533 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3157 3374 4238 1613
Size: 1544 217 864 4453
Fragments arranged by size:
4453 1544 864 217
PsiI TTA'TAA
Cuts at: 4648 4648
Size: 7078
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3677 3709 3677
Size: 32 7046
PstI C_TGCA'G
Cuts at: 830 2433 3042 3453 3546 3863 4089 4430 830
Size: 1603 609 411 93 317 226 341 3478
Fragments arranged by size:
3478 1603 609 411 341 317 226 93
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7078
PvuII CAG'CTG
Cuts at: 2323 2842 3043 2323
Size: 519 201 6358
Fragments arranged by size:
6358 519 201
SalI G'TCGA_C
Cuts at: 1936 4420 1936
Size: 2484 4594
SapI GCTCTTCn'nnn_
Cuts at: 2328 2328
Size: 7078
SbfI CC_TGCA'GG
Cuts at: 4430 4430
Size: 7078
ScaI AGT'ACT
Cuts at: 5326 7038 5326
Size: 1712 5366
SfcI C'TryA_G
Cuts at: 826 1045 2429 3038 3449 3542 3859 4085 4426
Size: 219 1384 609 411 93 317 226 341
Cuts at: 4426 5561 6242 6433 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1384 1135 681 609 411 341 317
226 219 191 93
SfoI GGC'GCC
Cuts at: 2450 2719 4082 2450
Size: 269 1363 5446
Fragments arranged by size:
5446 1363 269
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7078
SgrAI Cr'CCGG_yG
Cuts at: 1700 2714 1700
Size: 1014 6064
SmaI CCC'GGG
Cuts at: 1872 3395 4263 4405 1872
Size: 1523 868 142 4545
Fragments arranged by size:
4545 1523 868 142
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2271 5182 6083 6345 820
Size: 197 926 328 2911 901 262 1553
Fragments arranged by size:
2911 1553 926 901 328 262 197
SnaBI TAC'GTA
Cuts at: 493 4869 493
Size: 4376 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7078
SphI G_CATG'C
Cuts at: 1132 2817 4436 1132
Size: 1685 1619 3774
Fragments arranged by size:
3774 1685 1619
SspI AAT'ATT
Cuts at: 5 52 5002 5
Size: 47 4950 2081
Fragments arranged by size:
4950 2081 47
StuI AGG'CCT
Cuts at: 1517 2528 2639 3918 1517
Size: 1011 111 1279 4677
Fragments arranged by size:
4677 1279 1011 111
StyI C'CwwG_G
Cuts at: 513 1065 2019 2205 2328 2553 2739 3420 3457
Size: 552 954 186 123 225 186 681 37
Cuts at: 3457 3666 3717 3816 4059 4517 513
Size: 209 51 99 243 458 3074
Fragments arranged by size:
3074 954 681 552 458 243 225 209
186 186 123 99 51 37
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3911 5105 5122 5275 5460 5905 325
Size: 1255 2331 1194 17 153 185 445 1498
Fragments arranged by size:
2331 1498 1255 1194 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2625 3078 3621
Size: 274 80 33 51 1443 648 453 543
Cuts at: 3621 3726 5324 7036 96
Size: 105 1598 1712 138
Fragments arranged by size:
1712 1598 1443 648 543 453 274 138
105 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2118 2150 7038 7070 2118
Size: 32 4888 32 2126
Fragments arranged by size:
4888 2126 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7078
XbaI T'CTAG_A
Cuts at: 4414 4414
Size: 7078
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2320 4102 2320
Size: 1782 5296
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7078
XmaI C'CCGG_G
Cuts at: 1870 3393 4261 4403 1870
Size: 1523 868 142 4545
Fragments arranged by size:
4545 1523 868 142
XmnI GAAnn'nnTTC
Cuts at: 5207 5207
Size: 7078
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2725 4886 276
Size: 53 83 186 936 1191 2161 2468
Fragments arranged by size:
2468 2161 1191 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII
SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI BaeI BstZ17I Bsu36I DraIII
EcoICRI EcoRV FseI FspAI NruI PacI PmlI PpuMI
PspOMI PspXI RsrII SacI SacII SanDI SexAI SfiI
SrfI SwaI