Restriction map for
pFN21AE2791
(Circular) (Six-base) MAPSORT of: pFN21AE2791.seq Check: 9104 from: 1 to: 8200
pFN21AE2791 8200 bp
With 182 enzymes: SgfI *
February 20, 2021 22:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2196 2946 3842 4481 2196
Size: 750 896 639 5915
Fragments arranged by size:
5915 896 750 639
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4623 6010 278
Size: 53 83 186 936 3087 1387 2468
Fragments arranged by size:
3087 2468 1387 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5543 1937
Size: 3606 4594
Acc65I G'GTAC_C
Cuts at: 5521 5521
Size: 8200
AclI AA'CG_TT
Cuts at: 1563 6327 6700 1563
Size: 4764 373 3063
Fragments arranged by size:
4764 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2070 3017 3988 4258 6263 7647 7915 1632
Size: 438 947 971 270 2005 1384 268 1917
Fragments arranged by size:
2005 1917 1384 971 947 438 270 268
AfeI AGC'GCT
Cuts at: 4994 4994
Size: 8200
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8003
AflIII A'CryG_T
Cuts at: 1203 4944 4974 7099 8076 1203
Size: 3741 30 2125 977 1327
Fragments arranged by size:
3741 2125 1327 977 30
AgeI A'CCGG_T
Cuts at: 7031 7031
Size: 8200
AhdI GACnn_n'nnGTC
Cuts at: 6929 6929
Size: 8200
AleI CACnn'nnGTG
Cuts at: 1166 4904 5445 1166
Size: 3738 541 3921
Fragments arranged by size:
3921 3738 541
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3299 4499 1184
Size: 2115 1200 4885
Fragments arranged by size:
4885 2115 1200
AlwNI CAG_nnn'CTG
Cuts at: 2938 3912 4051 4684 5943 7515 2938
Size: 974 139 633 1259 1572 3623
Fragments arranged by size:
3623 1572 1259 974 633 139
ApaI G_GGCC'C
Cuts at: 2519 3494 4295 4305 4586 2519
Size: 975 801 10 281 6133
Fragments arranged by size:
6133 975 801 281 10
ApaLI G'TGCA_C
Cuts at: 6257 7413 6257
Size: 1156 7044
ApoI r'AATT_y
Cuts at: 1493 4181 5509 5735 5887 7996 1493
Size: 2688 1328 226 152 2109 1697
Fragments arranged by size:
2688 2109 1697 1328 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6754 160
Size: 872 5722 1606
Fragments arranged by size:
5722 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8200
AvaI C'yCGr_G
Cuts at: 1870 1943 2039 2575 2770 3182 3357 3708 4304
Size: 73 96 536 195 412 175 351 596
Cuts at: 4304 5279 5471 5525 1870
Size: 975 192 54 4545
Fragments arranged by size:
4545 975 596 536 412 351 195 192
175 96 73 54
BamHI G'GATC_C
Cuts at: 2310 5530 5897 2310
Size: 3220 367 4613
Fragments arranged by size:
4613 3220 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2131 2184 2824 3344 4120
Size: 325 217 627 344 53 640 520 776
Cuts at: 4120 5521 6976 618
Size: 1401 1455 1842
Fragments arranged by size:
1842 1455 1401 776 640 627 520 344
325 217 53
BanII G_rGCy'C
Cuts at: 1391 2519 2972 3060 3258 3494 4079 4132 4295
Size: 1128 453 88 198 236 585 53 163
Cuts at: 4295 4305 4586 5399 5519 1391
Size: 10 281 813 120 4072
Fragments arranged by size:
4072 1128 813 585 453 281 236 198
163 120 88 53 10
BbeI G_GCGC'C
Cuts at: 4124 4124
Size: 8200
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2058 4150 4615 5077 928
Size: 971 159 2092 465 462 4051
Fragments arranged by size:
4051 2092 971 465 462 159
BbvCI CC'TCA_GC
Cuts at: 2609 2609
Size: 8200
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2774 2808 4498 4532 6391
Size: 34 50 34 753 34 1690 34 1859
Cuts at: 6391 6425 1903
Size: 34 3678
Fragments arranged by size:
3678 1859 1690 753 50 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6092 7302 6092
Size: 1210 6990
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8200
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3650 3704 3854
Size: 107 122 71 631 605 1978 54 150
Cuts at: 3854 6811 136
Size: 2957 1525
Fragments arranged by size:
2957 1978 1525 631 605 150 122 107
71 54
BglII A'GATC_T
Cuts at: 3105 4752 8195 3105
Size: 1647 3443 3110
Fragments arranged by size:
3443 3110 1647
BlpI GC'TnA_GC
Cuts at: 5054 5100 5617 5054
Size: 46 517 7637
Fragments arranged by size:
7637 517 46
Bme1580I G_kGCm'C
Cuts at: 1790 2326 2519 2657 2829 3347 3494 4295 4305
Size: 536 193 138 172 518 147 801 10
Cuts at: 4305 4586 6261 7417 1790
Size: 281 1675 1156 2573
Fragments arranged by size:
2573 1675 1156 801 536 518 281 193
172 147 138 10
BmgBI CAC'GTC
Cuts at: 1318 2388 3121 3220 5368 1318
Size: 1070 733 99 2148 4150
Fragments arranged by size:
4150 2148 1070 733 99
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5931 6889 442
Size: 416 53 83 399 24 4514 958 1753
Fragments arranged by size:
4514 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8200
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2740 3983 2740
Size: 1243 6957
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2477 2636 2710 3164 3707 3841 4055 4364
Size: 1238 159 74 454 543 134 214 309
Cuts at: 4364 4730 4844 5141 5339 6860 1239
Size: 366 114 297 198 1521 2579
Fragments arranged by size:
2579 1521 1238 543 454 366 309 297
214 198 159 134 114 74
Bpu10I CC'TnA_GC
Cuts at: 2609 4131 5063 5192 2609
Size: 1522 932 129 5617
Fragments arranged by size:
5617 1522 932 129
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3044 3398 6325 7190 7488 3044
Size: 354 2927 865 298 3756
Fragments arranged by size:
3756 2927 865 354 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3406 5121 6863 882
Size: 617 1907 1715 1742 2219
Fragments arranged by size:
2219 1907 1742 1715 617
BsaAI yAC'GTr
Cuts at: 493 1204 4151 5991 493
Size: 711 2947 1840 2702
Fragments arranged by size:
2947 2702 1840 711
BsaBI GATnn'nnATC
Cuts at: 4556 8194 4556
Size: 3638 4562
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3162 3432 3861 4121
Size: 53 83 186 936 1629 270 429 260
Cuts at: 4121 4620 6007 6389 7024 275
Size: 499 1387 382 635 1451
Fragments arranged by size:
1629 1451 1387 936 635 499 429 382
270 260 186 83 53
BsaWI w'CCGG_w
Cuts at: 6633 7031 7305 7452 7863 6633
Size: 398 274 147 411 6970
Fragments arranged by size:
6970 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3116 3146 3386 3416 3740
Size: 30 165 30 1713 30 240 30 324
Cuts at: 3740 3770 1178
Size: 30 5608
Fragments arranged by size:
5608 1713 324 240 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2240 2474 2990 3161 3329 4121 5227 1186
Size: 1054 234 516 171 168 792 1106 4159
Fragments arranged by size:
4159 1106 1054 792 516 234 171 168
BseYI C'CCAG_C
Cuts at: 2065 2428 2864 3348 4579 4844 5460 7403 7899
Size: 363 436 484 1231 265 616 1943 496
Cuts at: 7899 2065
Size: 2366
Fragments arranged by size:
2366 1943 1231 616 496 484 436 363
265
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2426 3377 3824 4673 4979 1163
Size: 573 690 951 447 849 306 4384
Fragments arranged by size:
4384 951 849 690 573 447 306
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3460 4705 5650 6411 7439 1540
Size: 275 186 1459 1245 945 761 1028 2301
Fragments arranged by size:
2301 1459 1245 1028 945 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2160 2300 3060 3258 4734 5106 5118 5349 5399
Size: 140 760 198 1476 372 12 231 50
Cuts at: 5399 6261 6346 7417 2160
Size: 862 85 1071 2943
Fragments arranged by size:
2943 1476 1071 862 760 372 231 198
140 85 50 12
BsmI GAATG_Cn'
Cuts at: 2451 5711 5804 2451
Size: 3260 93 4847
Fragments arranged by size:
4847 3260 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3683 3683
Size: 8200
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2160 2300 2326 2519 2657 2829 2972
Size: 399 370 140 26 193 138 172 143
Cuts at: 2972 3060 3258 3347 3494 4079 4132 4295 4305
Size: 88 198 89 147 585 53 163 10
Cuts at: 4305 4586 4734 5106 5118 5349 5399 5519 6261
Size: 281 148 372 12 231 50 120 742
Cuts at: 6261 6346 7417 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 585 399 372 370 281
231 198 193 172 163 148 147 143
140 138 120 89 88 85 53 50
26 12 10
BspEI T'CCGG_A
Cuts at: 7863 7863
Size: 8200
BspHI T'CATG_A
Cuts at: 4914 6089 7819 4914
Size: 1175 1730 5295
Fragments arranged by size:
5295 1730 1175
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2175 2196 2946 3303 3842 4050 4481 4755
Size: 1331 21 750 357 539 208 431 274
Cuts at: 4755 4929 5139 5555 844
Size: 174 210 416 3489
Fragments arranged by size:
3489 1331 750 539 431 416 357 274
210 208 174 21
BsrBI CCG'CTC
Cuts at: 1394 1823 2139 3264 3736 6087 1394
Size: 429 316 1125 472 2351 3507
Fragments arranged by size:
3507 2351 1125 472 429 316
BsrDI GCAATG_nn'
Cuts at: 65 1248 5265 6695 6869 65
Size: 1183 4017 1430 174 1396
Fragments arranged by size:
4017 1430 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2055 2191 3335 3379 3459 4737 6844
Size: 110 245 136 1144 44 80 1278 2107
Cuts at: 6844 7031 1700
Size: 187 2869
Fragments arranged by size:
2869 2107 1278 1144 245 187 136 110
80 44
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8200
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8200
BssSI C'ACGA_G
Cuts at: 3395 3596 4655 5018 5348 6260 7272 3395
Size: 201 1059 363 330 912 1012 4323
Fragments arranged by size:
4323 1059 1012 912 363 330 201
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2362 794
Size: 1568 6632
BstBI TT'CG_AA
Cuts at: 7004 7004
Size: 8200
BstEII G'GTnAC_C
Cuts at: 1184 4136 4309 4882 4897 1184
Size: 2952 173 573 15 4487
Fragments arranged by size:
4487 2952 573 173 15
BstXI CCAn_nnnn'nTGG
Cuts at: 2206 2713 2734 4864 5447 2206
Size: 507 21 2130 583 4959
Fragments arranged by size:
4959 2130 583 507 21
BstYI r'GATC_y
Cuts at: 1972 2310 2670 2721 3105 3909 4716 4752 4764
Size: 338 360 51 384 804 807 36 12
Cuts at: 4764 5530 5897 6282 6299 7751 7837 7849 8195
Size: 766 367 385 17 1452 86 12 346
Cuts at: 8195 1972
Size: 1977
Fragments arranged by size:
1977 1452 807 804 766 385 384 367
360 346 338 86 51 36 17 12
12
Bsu36I CC'TnA_GG
Cuts at: 2493 3039 4022 4297 2493
Size: 546 983 275 6396
Fragments arranged by size:
6396 983 546 275
BtgI C'CryG_G
Cuts at: 513 1065 2733 2750 3069 3369 3507 3621 4521
Size: 552 1668 17 319 300 138 114 900
Cuts at: 4521 5358 513
Size: 837 3355
Fragments arranged by size:
3355 1668 900 837 552 319 300 138
114 17
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2601 2764 3076 4912 487
Size: 683 56 1375 163 312 1836 3775
Fragments arranged by size:
3775 1836 1375 683 312 163 56
BtsI GCAGTG_nn'
Cuts at: 4814 5716 6510 6530 4814
Size: 902 794 20 6484
Fragments arranged by size:
6484 902 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8200
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8165
DraI TTT'AAA
Cuts at: 5504 5504
Size: 8200
DraIII CAC_nnn'GTG
Cuts at: 4390 5257 4390
Size: 867 7333
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3167 5153 7207 809
Size: 507 1851 1986 2054 1802
Fragments arranged by size:
2054 1986 1851 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2340 2379 2835
Size: 54 1425 50 275 18 510 39 456
Cuts at: 2835 3066 3222 3333 3457 3461 3495 3603 3651
Size: 231 156 111 124 4 34 108 48
Cuts at: 3651 3846 3927 4443 4504 4702 4759 5479 5647
Size: 195 81 516 61 198 57 720 168
Cuts at: 5647 6536 8165 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 720 516 510 456 275
231 198 195 168 156 124 111 108
81 61 57 54 50 48 43 39
34 18 4
EagI C'GGCC_G
Cuts at: 1537 1812 3457 4702 5647 1537
Size: 275 1645 1245 945 4090
Fragments arranged by size:
4090 1645 1245 945 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3293 3536 3758 4067 4247 4640 6130 1353
Size: 1940 243 222 309 180 393 1490 3423
Fragments arranged by size:
3423 1940 1490 393 309 243 222 180
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3497 4192 4712 5066 6787 7161
Size: 1064 138 1908 695 520 354 1721 374
Cuts at: 7161 7307 387
Size: 146 1280
Fragments arranged by size:
1908 1721 1280 1064 695 520 374 354
146 138
EcoICRI GAG'CTC
Cuts at: 3058 3256 5397 3058
Size: 198 2141 5861
Fragments arranged by size:
5861 2141 198
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2070 2477 2636 2710 3017 3164 3707
Size: 393 438 407 159 74 307 147 543
Cuts at: 3707 3841 3988 4055 4258 4364 4730 4844 5141
Size: 134 147 67 203 106 366 114 297
Cuts at: 5141 5339 6263 6860 7647 7915 1239
Size: 198 924 597 787 268 1524
Fragments arranged by size:
1524 924 787 597 543 438 407 393
366 307 297 268 203 198 159 147
147 134 114 106 74 67
EcoNI CCTnn'n_nnAGG
Cuts at: 2716 3146 3395 2716
Size: 430 249 7521
Fragments arranged by size:
7521 430 249
EcoO109I rG'GnC_Cy
Cuts at: 2021 2515 2516 2570 3490 3562 4039 4048 4291
Size: 494 1 54 920 72 477 9 243
Cuts at: 4291 4292 4301 4583 2021
Size: 1 9 282 5638
Fragments arranged by size:
5638 920 494 477 282 243 72 54
9 9 1 1
EcoRI G'AATT_C
Cuts at: 5509 5887 5509
Size: 378 7822
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3889 4639 5203 3889
Size: 750 564 6886
Fragments arranged by size:
6886 750 564
FseI GG_CCGG'CC
Cuts at: 3339 3463 3339
Size: 124 8076
FspI TGC'GCA
Cuts at: 2270 6706 2270
Size: 4436 3764
HaeII r_GCGC'y
Cuts at: 1678 3005 4124 4194 4685 4996 5026 7347 1678
Size: 1327 1119 70 491 311 30 2321 2531
Fragments arranged by size:
2531 2321 1327 1119 491 311 70 30
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2295 2327 3116 3148 3740
Size: 32 135 32 723 32 789 32 592
Cuts at: 3740 3772 4820 4852 6841 6873 6915 6947 1373
Size: 32 1048 32 1989 32 42 32 2626
Fragments arranged by size:
2626 1989 1048 789 723 592 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2089 5544 5790 669
Size: 988 281 151 3455 246 3079
Fragments arranged by size:
3455 3079 988 281 246 151
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8200
HpaI GTT'AAC
Cuts at: 5790 5790
Size: 8200
KasI G'GCGC_C
Cuts at: 4120 4120
Size: 8200
KpnI G_GTAC'C
Cuts at: 5525 5525
Size: 8200
MfeI C'AATT_G
Cuts at: 5799 5799
Size: 8200
MluI A'CGCG_T
Cuts at: 8076 8076
Size: 8200
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 7314 7498 1305
Size: 6009 184 2007
Fragments arranged by size:
6009 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2342 2381 3605 3848 4445
Size: 54 1425 343 510 39 1224 243 597
Cuts at: 4445 5481 10
Size: 1036 2729
Fragments arranged by size:
2729 1425 1224 1036 597 510 343 243
54 39
MslI CAynn'nnrTG
Cuts at: 518 1166 2558 4695 4904 4931 5445 6158 6517
Size: 648 1392 2137 209 27 514 713 359
Cuts at: 6517 6676 518
Size: 159 2042
Fragments arranged by size:
2137 2042 1392 713 648 514 359 209
159 27
MspA1I CmG'CkG
Cuts at: 1606 3304 3371 3816 4081 4579 5425 5616 6293
Size: 1698 67 445 265 498 846 191 677
Cuts at: 6293 7441 7686 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1698 1148 846 677 498 445 265
245 191 67
NaeI GCC'GGC
Cuts at: 2193 3337 3461 2193
Size: 1144 124 6932
Fragments arranged by size:
6932 1144 124
NarI GG'CG_CC
Cuts at: 4121 4121
Size: 8200
NcoI C'CATG_G
Cuts at: 513 1065 3621 4521 513
Size: 552 2556 900 4192
Fragments arranged by size:
4192 2556 900 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8200
NgoMIV G'CCGG_C
Cuts at: 2191 3335 3459 2191
Size: 1144 124 6932
Fragments arranged by size:
6932 1144 124
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8200
NotI GC'GGCC_GC
Cuts at: 5647 5647
Size: 8200
NspI r_CATG'y
Cuts at: 1132 1229 2351 4978 5558 7103 1132
Size: 97 1122 2627 580 1545 2229
Fragments arranged by size:
2627 2229 1545 1122 580 97
PasI CC'CwG_GG
Cuts at: 1348 4288 5377 1348
Size: 2940 1089 4171
Fragments arranged by size:
4171 2940 1089
PciI A'CATG_T
Cuts at: 4974 7099 4974
Size: 2125 6075
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2377 2821 3601 3912 5257 5428 1108
Size: 1269 444 780 311 1345 171 3880
Fragments arranged by size:
3880 1345 1269 780 444 311 171
PfoI T'CCnGG_A
Cuts at: 2029 2029
Size: 8200
PmeI GTTT'AAAC
Cuts at: 5504 5504
Size: 8200
PmlI CAC'GTG
Cuts at: 4151 4151
Size: 8200
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2909 2941 6269 6301 7782 7814 2909
Size: 32 3328 32 1481 32 3295
Fragments arranged by size:
3328 3295 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3562 4039 4048 3562
Size: 477 9 7714
Fragments arranged by size:
7714 477 9
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8200
PsiI TTA'TAA
Cuts at: 5770 5770
Size: 8200
PspOMI G'GGCC_C
Cuts at: 2515 3490 4291 4301 4582 2515
Size: 975 801 10 281 6133
Fragments arranged by size:
6133 975 801 281 10
PstI C_TGCA'G
Cuts at: 830 2916 3252 3303 3429 3588 4047 4095 4478
Size: 2086 336 51 126 159 459 48 383
Cuts at: 4478 4752 5552 830
Size: 274 800 3478
Fragments arranged by size:
3478 2086 800 459 383 336 274 159
126 51 48
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8200
PvuII CAG'CTG
Cuts at: 4579 5425 4579
Size: 846 7354
SacI G_AGCT'C
Cuts at: 3060 3258 5399 3060
Size: 198 2141 5861
Fragments arranged by size:
5861 2141 198
SacII CC_GC'GG
Cuts at: 3372 3372
Size: 8200
SalI G'TCGA_C
Cuts at: 1936 5542 1936
Size: 3606 4594
SapI GCTCTTCn'nnn_
Cuts at: 3293 3536 4067 3293
Size: 243 531 7426
Fragments arranged by size:
7426 531 243
SbfI CC_TGCA'GG
Cuts at: 3429 4047 4478 5552 3429
Size: 618 431 1074 6077
Fragments arranged by size:
6077 1074 618 431
ScaI AGT'ACT
Cuts at: 6448 8160 6448
Size: 1712 6488
SexAI A'CCwGG_T
Cuts at: 2202 4386 2202
Size: 2184 6016
SfcI C'TryA_G
Cuts at: 826 1045 2912 3248 3299 3425 3584 3766 4043
Size: 219 1867 336 51 126 159 182 277
Cuts at: 4043 4091 4474 4748 5548 6683 7364 7555 826
Size: 48 383 274 800 1135 681 191 1471
Fragments arranged by size:
1867 1471 1135 800 681 383 336 277
274 219 191 182 159 126 51 48
SfiI GGCCn_nnn'nGGCC
Cuts at: 3704 3704
Size: 8200
SfoI GGC'GCC
Cuts at: 4122 4122
Size: 8200
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8200
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8200
SmaI CCC'GGG
Cuts at: 1872 2041 2577 3359 5473 5527 1872
Size: 169 536 782 2114 54 4545
Fragments arranged by size:
4545 2114 782 536 169 54
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3059 3413 6304 7205 7467 820
Size: 197 926 1116 354 2891 901 262 1553
Fragments arranged by size:
2891 1553 1116 926 901 354 262 197
SnaBI TAC'GTA
Cuts at: 493 5991 493
Size: 5498 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8200
SphI G_CATG'C
Cuts at: 1132 5558 1132
Size: 4426 3774
SspI AAT'ATT
Cuts at: 5 52 6124 5
Size: 47 6072 2081
Fragments arranged by size:
6072 2081 47
StuI AGG'CCT
Cuts at: 1517 3869 3896 4032 1517
Size: 2352 27 136 5685
Fragments arranged by size:
5685 2352 136 27
StyI C'CwwG_G
Cuts at: 513 1065 2017 2394 2712 2829 3621 3864 4521
Size: 552 952 377 318 117 792 243 657
Cuts at: 4521 5639 513
Size: 1118 3074
Fragments arranged by size:
3074 1118 952 792 657 552 377 318
243 117
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2210 3362 4155 4454 4691 4823 4827
Size: 1255 630 1152 793 299 237 132 4
Cuts at: 4827 6227 6244 6397 6582 7027 325
Size: 1400 17 153 185 445 1498
Fragments arranged by size:
1498 1400 1255 1152 793 630 445 299
237 185 153 132 17 4
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4227 6446 8158
Size: 274 80 33 51 1443 2250 2219 1712
Cuts at: 8158 96
Size: 138
Fragments arranged by size:
2250 2219 1712 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3110 3121 3142 3153 3386 3418 4417 4449 8160
Size: 11 21 11 233 32 999 32 3711
Cuts at: 8160 8192 3110
Size: 32 3118
Fragments arranged by size:
3711 3118 999 233 32 32 32 21
11 11
Tth111I GACn'n_nGTC
Cuts at: 1316 2987 1316
Size: 1671 6529
XbaI T'CTAG_A
Cuts at: 5536 5536
Size: 8200
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2401 4528 4594 2401
Size: 2127 66 6007
Fragments arranged by size:
6007 2127 66
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8200
XmaI C'CCGG_G
Cuts at: 1870 2039 2575 3357 5471 5525 1870
Size: 169 536 782 2114 54 4545
Fragments arranged by size:
4545 2114 782 536 169 54
XmnI GAAnn'nnTTC
Cuts at: 4397 6329 4397
Size: 1932 6268
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4621 6008 276
Size: 53 83 186 936 3087 1387 2468
Fragments arranged by size:
3087 2468 1387 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I EcoRV
FspAI NruI NsiI PacI PspXI PsrI RsrII SanDI
SrfI SwaI