Restriction map for
pFN21AE2787
(Circular) (Six-base) MAPSORT of: pFN21AE2787.seq Check: 9910 from: 1 to: 6436
pFN21AE2787 6436 bp
With 182 enzymes: SgfI *
February 20, 2021 22:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3355 3355
Size: 6436
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4246 278
Size: 53 83 186 936 2710 2468
Fragments arranged by size:
2710 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3333 3779 1937
Size: 1396 446 4594
Fragments arranged by size:
4594 1396 446
Acc65I G'GTAC_C
Cuts at: 3757 3757
Size: 6436
AclI AA'CG_TT
Cuts at: 1563 4563 4936 1563
Size: 3000 373 3063
Fragments arranged by size:
3063 3000 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3173 3230 4499 5883 6151 1632
Size: 1541 57 1269 1384 268 1917
Fragments arranged by size:
1917 1541 1384 1269 268 57
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6239
AflIII A'CryG_T
Cuts at: 1203 2781 5335 6312 1203
Size: 1578 2554 977 1327
Fragments arranged by size:
2554 1578 1327 977
AgeI A'CCGG_T
Cuts at: 5267 5267
Size: 6436
AhdI GACnn_n'nnGTC
Cuts at: 5165 5165
Size: 6436
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6436
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6436
AlwNI CAG_nnn'CTG
Cuts at: 4179 5751 4179
Size: 1572 4864
ApaLI G'TGCA_C
Cuts at: 4493 5649 4493
Size: 1156 5280
ApoI r'AATT_y
Cuts at: 1493 3063 3264 3425 3745 3971 4123 6232 1493
Size: 1570 201 161 320 226 152 2109 1697
Fragments arranged by size:
2109 1697 1570 320 226 201 161 152
AseI AT'TA_AT
Cuts at: 160 1032 3309 4990 160
Size: 872 2277 1681 1606
Fragments arranged by size:
2277 1681 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6436
AvaI C'yCGr_G
Cuts at: 1870 1943 3761 1870
Size: 73 1818 4545
Fragments arranged by size:
4545 1818 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2783 2816 2783
Size: 33 6403
BamHI G'GATC_C
Cuts at: 3766 4133 3766
Size: 367 6069
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2038 2197 2431 2540 3367
Size: 325 217 627 251 159 234 109 827
Cuts at: 3367 3757 5212 618
Size: 390 1455 1842
Fragments arranged by size:
1842 1455 827 627 390 325 251 234
217 159 109
BanII G_rGCy'C
Cuts at: 1391 2015 2270 3755 1391
Size: 624 255 1485 4072
Fragments arranged by size:
4072 1485 624 255
BbeI G_GCGC'C
Cuts at: 2042 2201 2435 2544 2042
Size: 159 234 109 5934
Fragments arranged by size:
5934 234 159 109
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2713 3035 3190 928
Size: 971 814 322 155 4174
Fragments arranged by size:
4174 971 814 322 155
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3386 3420 4627 4661 1903
Size: 34 1449 34 1207 34 3678
Fragments arranged by size:
3678 1449 1207 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4328 5538 4328
Size: 1210 5226
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6436
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2078 2231 5047
Size: 107 122 71 631 605 406 153 2816
Cuts at: 5047 136
Size: 1525
Fragments arranged by size:
2816 1525 631 605 406 153 122 107
71
BglII A'GATC_T
Cuts at: 3399 6431 3399
Size: 3032 3404
BlpI GC'TnA_GC
Cuts at: 2327 3853 2327
Size: 1526 4910
Bme1580I G_kGCm'C
Cuts at: 1790 3720 4497 5653 1790
Size: 1930 777 1156 2573
Fragments arranged by size:
2573 1930 1156 777
BmgBI CAC'GTC
Cuts at: 1318 2169 1318
Size: 851 5585
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4167 5125 442
Size: 416 53 83 399 24 2750 958 1753
Fragments arranged by size:
2750 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6436
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2414 3659 2414
Size: 1245 5191
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2162 5096 1239
Size: 923 2934 2579
Fragments arranged by size:
2934 2579 923
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4561 5426 5724 4561
Size: 865 298 5273
Fragments arranged by size:
5273 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3681 5099 882
Size: 617 2182 1418 2219
Fragments arranged by size:
2219 2182 1418 617
BsaAI yAC'GTr
Cuts at: 493 1204 4227 493
Size: 711 3023 2702
Fragments arranged by size:
3023 2702 711
BsaBI GATnn'nnATC
Cuts at: 3389 6430 3389
Size: 3041 3395
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2039 2198 2432 2445
Size: 53 83 186 936 506 159 234 13
Cuts at: 2445 2541 3495 4243 4625 5260 275
Size: 96 954 748 382 635 1451
Fragments arranged by size:
1451 954 936 748 635 506 382 234
186 159 96 83 53 13
BsaWI w'CCGG_w
Cuts at: 2566 4869 5267 5541 5688 6099 2566
Size: 2303 398 274 147 411 2903
Fragments arranged by size:
2903 2303 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2740 2770 3067 3097 1178
Size: 30 165 30 1337 30 297 30 4517
Fragments arranged by size:
4517 1337 297 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3075 1186
Size: 1889 4547
BseYI C'CCAG_C
Cuts at: 2047 2084 2208 2302 2347 5639 6135 2047
Size: 37 124 94 45 3292 496 2348
Fragments arranged by size:
3292 2348 496 124 94 45 37
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2410 1163
Size: 573 674 5189
Fragments arranged by size:
5189 674 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2073 3886 4647 5675 1540
Size: 275 186 72 1813 761 1028 2301
Fragments arranged by size:
2301 1813 1028 761 275 186 72
BsiHKAI G_wGCw'C
Cuts at: 3132 3248 4497 4582 5653 3132
Size: 116 1249 85 1071 3915
Fragments arranged by size:
3915 1249 1071 116 85
BsmI GAATG_Cn'
Cuts at: 3947 4040 3947
Size: 93 6343
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2015 2270 3132 3248 3720 3755 4497
Size: 399 225 255 862 116 472 35 742
Cuts at: 4497 4582 5653 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 862 742 472 399 255 225
116 85 35
BspEI T'CCGG_A
Cuts at: 2566 6099 2566
Size: 3533 2903
BspHI T'CATG_A
Cuts at: 4325 6055 4325
Size: 1730 4706
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2352 3355 3791 844
Size: 1508 1003 436 3489
Fragments arranged by size:
3489 1508 1003 436
BsrBI CCG'CTC
Cuts at: 1394 1823 2035 4323 1394
Size: 429 212 2288 3507
Fragments arranged by size:
3507 2288 429 212
BsrDI GCAATG_nn'
Cuts at: 65 1248 2640 4931 5105 65
Size: 1183 1392 2291 174 1396
Fragments arranged by size:
2291 1396 1392 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2100 2467 5080 5267 1700
Size: 110 290 367 2613 187 2869
Fragments arranged by size:
2869 2613 367 290 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6436
BssHII G'CGCG_C
Cuts at: 1925 2052 2104 2146 2217 2312 2314 2411 1925
Size: 127 52 42 71 95 2 97 5950
Fragments arranged by size:
5950 127 97 95 71 52 42 2
BssSI C'ACGA_G
Cuts at: 4496 5508 4496
Size: 1012 5424
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3379 794
Size: 2585 3851
BstBI TT'CG_AA
Cuts at: 5240 5240
Size: 6436
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6436
BstXI CCAn_nnnn'nTGG
Cuts at: 2843 2843
Size: 6436
BstYI r'GATC_y
Cuts at: 1972 3399 3766 4133 4518 4535 5987 6073 6085
Size: 1427 367 367 385 17 1452 86 12
Cuts at: 6085 6431 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1427 385 367 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2073 3222 513
Size: 552 939 69 1149 3727
Fragments arranged by size:
3727 1149 939 552 69
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5697
Fragments arranged by size:
5697 683 56
BtsI GCAGTG_nn'
Cuts at: 2773 3952 4746 4766 2773
Size: 1179 794 20 4443
Fragments arranged by size:
4443 1179 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6436
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6401
DraI TTT'AAA
Cuts at: 3740 3740
Size: 6436
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3164 5443 809
Size: 507 1848 2279 1802
Fragments arranged by size:
2279 1848 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2070 2289 2373
Size: 54 1425 50 275 18 240 219 84
Cuts at: 2373 2469 2508 2731 3883 4772 6401 8
Size: 96 39 223 1152 889 1629 43
Fragments arranged by size:
1629 1425 1152 889 275 240 223 219
96 84 54 50 43 39 18
EagI C'GGCC_G
Cuts at: 1537 1812 2070 3883 1537
Size: 275 258 1813 4090
Fragments arranged by size:
4090 1813 275 258
EarI CTCTTCn'nnn_
Cuts at: 1353 2249 2925 3206 4366 1353
Size: 896 676 281 1160 3423
Fragments arranged by size:
3423 1160 896 676 281
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5023 5397 5543 387
Size: 1064 138 3434 374 146 1280
Fragments arranged by size:
3434 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2162 3173 3230 4499 5096 5883 6151
Size: 393 530 1011 57 1269 597 787 268
Cuts at: 6151 1239
Size: 1524
Fragments arranged by size:
1524 1269 1011 787 597 530 393 268
57
EcoRI G'AATT_C
Cuts at: 3745 4123 3745
Size: 378 6058
FspI TGC'GCA
Cuts at: 4942 4942
Size: 6436
HaeII r_GCGC'y
Cuts at: 1678 2042 2201 2237 2339 2435 2544 2836 5583
Size: 364 159 36 102 96 109 292 2747
Cuts at: 5583 1678
Size: 2531
Fragments arranged by size:
2747 2531 364 292 159 109 102 96
36
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3547 3579 5077 5109 5151
Size: 32 135 32 1975 32 1498 32 42
Cuts at: 5151 5183 1373
Size: 32 2626
Fragments arranged by size:
2626 1975 1498 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3780 4026 669
Size: 988 281 1842 246 3079
Fragments arranged by size:
3079 1842 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6436
HpaI GTT'AAC
Cuts at: 4026 4026
Size: 6436
KasI G'GCGC_C
Cuts at: 2038 2197 2431 2540 2038
Size: 159 234 109 5934
Fragments arranged by size:
5934 234 159 109
KpnI G_GTAC'C
Cuts at: 3761 3761
Size: 6436
MfeI C'AATT_G
Cuts at: 4035 4035
Size: 6436
MluI A'CGCG_T
Cuts at: 6312 6312
Size: 6436
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2196 5550 5734 1305
Size: 891 3354 184 2007
Fragments arranged by size:
3354 2007 891 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2510 2733 10
Size: 54 1425 343 678 223 3713
Fragments arranged by size:
3713 1425 678 343 223 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2780 4394 4753 4912 518
Size: 648 1614 1614 359 159 2042
Fragments arranged by size:
2042 1614 1614 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2044 2075 2368 2457 3440 3852 4529 5677
Size: 438 31 293 89 983 412 677 1148
Cuts at: 5677 5922 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 983 677 438 412 293 245
89 31
NaeI GCC'GGC
Cuts at: 2102 2102
Size: 6436
NarI GG'CG_CC
Cuts at: 2039 2198 2432 2541 2039
Size: 159 234 109 5934
Fragments arranged by size:
5934 234 159 109
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4945
Fragments arranged by size:
4945 939 552
NdeI CA'TA_TG
Cuts at: 387 3028 387
Size: 2641 3795
NgoMIV G'CCGG_C
Cuts at: 2100 2100
Size: 6436
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6436
NotI GC'GGCC_GC
Cuts at: 2070 3883 2070
Size: 1813 4623
NspI r_CATG'y
Cuts at: 1132 1229 2356 2785 3232 3794 5339 1132
Size: 97 1127 429 447 562 1545 2229
Fragments arranged by size:
2229 1545 1127 562 447 429 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6436
PciI A'CATG_T
Cuts at: 2781 5335 2781
Size: 2554 3882
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3552 1108
Size: 2444 3992
PmeI GTTT'AAAC
Cuts at: 3740 3740
Size: 6436
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4505 4537 6018 6050 4505
Size: 32 1481 32 4891
Fragments arranged by size:
4891 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6436
PsiI TTA'TAA
Cuts at: 4006 4006
Size: 6436
PstI C_TGCA'G
Cuts at: 830 3103 3262 3788 830
Size: 2273 159 526 3478
Fragments arranged by size:
3478 2273 526 159
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6436
PvuII CAG'CTG
Cuts at: 3440 3440
Size: 6436
SacII CC_GC'GG
Cuts at: 2076 2076
Size: 6436
SalI G'TCGA_C
Cuts at: 1936 3778 1936
Size: 1842 4594
SapI GCTCTTCn'nnn_
Cuts at: 2249 2249
Size: 6436
SbfI CC_TGCA'GG
Cuts at: 3788 3788
Size: 6436
ScaI AGT'ACT
Cuts at: 3377 4684 6396 3377
Size: 1307 1712 3417
Fragments arranged by size:
3417 1712 1307
SexAI A'CCwGG_T
Cuts at: 2175 2547 2175
Size: 372 6064
SfcI C'TryA_G
Cuts at: 826 1045 3099 3258 3784 4919 5600 5791 826
Size: 219 2054 159 526 1135 681 191 1471
Fragments arranged by size:
2054 1471 1135 681 526 219 191 159
SfoI GGC'GCC
Cuts at: 2040 2199 2433 2542 2040
Size: 159 234 109 5934
Fragments arranged by size:
5934 234 159 109
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6436
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6436
SmaI CCC'GGG
Cuts at: 1872 3763 1872
Size: 1891 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4540 5441 5703 820
Size: 197 926 2597 901 262 1553
Fragments arranged by size:
2597 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4227 493
Size: 3734 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6436
SphI G_CATG'C
Cuts at: 1132 2356 3794 1132
Size: 1224 1438 3774
Fragments arranged by size:
3774 1438 1224
SspI AAT'ATT
Cuts at: 5 52 3073 3583 4360 5
Size: 47 3021 510 777 2081
Fragments arranged by size:
3021 2081 777 510 47
StuI AGG'CCT
Cuts at: 1517 3420 1517
Size: 1903 4533
StyI C'CwwG_G
Cuts at: 513 1065 2004 3875 513
Size: 552 939 1871 3074
Fragments arranged by size:
3074 1871 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4463 4480 4633 4818 5263 325
Size: 1255 2883 17 153 185 445 1498
Fragments arranged by size:
2883 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3375 4682 6394
Size: 274 80 33 51 1443 1398 1307 1712
Cuts at: 6394 96
Size: 138
Fragments arranged by size:
1712 1443 1398 1307 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2740 2772 6396 6428 2740
Size: 32 3624 32 2748
Fragments arranged by size:
3624 2748 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6436
XbaI T'CTAG_A
Cuts at: 3772 3772
Size: 6436
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2822 2822
Size: 6436
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6436
XmaI C'CCGG_G
Cuts at: 1870 3761 1870
Size: 1891 4545
XmnI GAAnn'nnTTC
Cuts at: 4565 4565
Size: 6436
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4244 276
Size: 53 83 186 936 2710 2468
Fragments arranged by size:
2710 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI PvuII SacII SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BbvCI BfrBI Bpu10I
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I
EcoRV FalI FseI FspAI NruI NsiI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SanDI
SfiI SrfI SwaI