Restriction map for
pFN21AE2786
(Circular) (Six-base) MAPSORT of: pFN21AE2786.seq Check: 6801 from: 1 to: 7513
pFN21AE2786 7513 bp
With 182 enzymes: SgfI *
February 20, 2021 22:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2999 3992 4351 2999
Size: 993 359 6161
Fragments arranged by size:
6161 993 359
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5323 278
Size: 53 83 186 936 3787 2468
Fragments arranged by size:
3787 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3711 4856 1937
Size: 1774 1145 4594
Fragments arranged by size:
4594 1774 1145
Acc65I G'GTAC_C
Cuts at: 4834 4834
Size: 7513
AclI AA'CG_TT
Cuts at: 1563 5640 6013 1563
Size: 4077 373 3063
Fragments arranged by size:
4077 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2541 2750 3574 5576 6960 7228 1632
Size: 909 209 824 2002 1384 268 1917
Fragments arranged by size:
2002 1917 1384 909 824 268 209
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7316
AflIII A'CryG_T
Cuts at: 1203 6412 7389 1203
Size: 5209 977 1327
Fragments arranged by size:
5209 1327 977
AgeI A'CCGG_T
Cuts at: 2943 6344 2943
Size: 3401 4112
AhdI GACnn_n'nnGTC
Cuts at: 6242 6242
Size: 7513
AleI CACnn'nnGTG
Cuts at: 1166 3539 1166
Size: 2373 5140
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2111 1184
Size: 927 6586
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2165 2197 2165
Size: 32 7481
AlwNI CAG_nnn'CTG
Cuts at: 4392 5256 6828 4392
Size: 864 1572 5077
Fragments arranged by size:
5077 1572 864
ApaI G_GGCC'C
Cuts at: 2154 2848 3488 3953 4038 4215 4471 4663 2154
Size: 694 640 465 85 177 256 192 5004
Fragments arranged by size:
5004 694 640 465 256 192 177 85
ApaLI G'TGCA_C
Cuts at: 3119 3542 3911 5570 6726 3119
Size: 423 369 1659 1156 3906
Fragments arranged by size:
3906 1659 1156 423 369
ApoI r'AATT_y
Cuts at: 1493 2749 4822 5048 5200 7309 1493
Size: 1256 2073 226 152 2109 1697
Fragments arranged by size:
2109 2073 1697 1256 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6067 160
Size: 872 5035 1606
Fragments arranged by size:
5035 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7513
AvaI C'yCGr_G
Cuts at: 1870 1943 2017 2126 2607 3480 3841 3850 3893
Size: 73 74 109 481 873 361 9 43
Cuts at: 3893 3964 4064 4463 4470 4623 4804 4838 1870
Size: 71 100 399 7 153 181 34 4545
Fragments arranged by size:
4545 873 481 399 361 181 153 109
100 74 73 71 43 34 9 7
AvrII C'CTAG_G
Cuts at: 2422 2422
Size: 7513
BamHI G'GATC_C
Cuts at: 2616 4843 5210 2616
Size: 2227 367 4919
Fragments arranged by size:
4919 2227 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2025 2823 4834 6289 618
Size: 325 217 627 238 798 2011 1455 1842
Fragments arranged by size:
2011 1842 1455 798 627 325 238 217
BanII G_rGCy'C
Cuts at: 1391 2154 2213 2519 2848 3488 3815 3842 3884
Size: 763 59 306 329 640 327 27 42
Cuts at: 3884 3903 3953 4038 4065 4152 4215 4232 4254
Size: 19 50 85 27 87 63 17 22
Cuts at: 4254 4471 4642 4663 4832 1391
Size: 217 171 21 169 4072
Fragments arranged by size:
4072 763 640 329 327 306 217 171
169 87 85 63 59 50 42 27
27 22 21 19 17
BbeI G_GCGC'C
Cuts at: 2029 2827 2029
Size: 798 6715
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2529 928
Size: 971 630 5912
Fragments arranged by size:
5912 971 630
BbvCI CC'TCA_GC
Cuts at: 3434 3773 3434
Size: 339 7174
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5704 5738 1903
Size: 34 3767 34 3678
Fragments arranged by size:
3767 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4582 5405 6615 4582
Size: 823 1210 5480
Fragments arranged by size:
5480 1210 823
BclI T'GATC_A
Cuts at: 1548 2500 1548
Size: 952 6561
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2076 2217 2250
Size: 107 122 71 631 605 404 141 33
Cuts at: 2250 3830 4763 6124 136
Size: 1580 933 1361 1525
Fragments arranged by size:
1580 1525 1361 933 631 605 404 141
122 107 71 33
BglII A'GATC_T
Cuts at: 4778 7508 4778
Size: 2730 4783
BlpI GC'TnA_GC
Cuts at: 2279 4930 2279
Size: 2651 4862
Bme1580I G_kGCm'C
Cuts at: 1790 2154 2848 3123 3488 3546 3915 3953 4038
Size: 364 694 275 365 58 369 38 85
Cuts at: 4038 4215 4471 4663 4726 5574 6730 1790
Size: 177 256 192 63 848 1156 2573
Fragments arranged by size:
2573 1156 848 694 369 365 364 275
256 192 177 85 63 58 38
BmgBI CAC'GTC
Cuts at: 1318 2874 3582 1318
Size: 1556 708 5249
Fragments arranged by size:
5249 1556 708
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2324 3388 4249
Size: 416 53 83 399 24 907 1064 861
Cuts at: 4249 5244 6202 442
Size: 995 958 1753
Fragments arranged by size:
1753 1064 995 958 907 861 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7513
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4426 4426
Size: 7513
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2915 3329 3587 3679 4013 4043 4712 6173
Size: 1676 414 258 92 334 30 669 1461
Cuts at: 6173 1239
Size: 2579
Fragments arranged by size:
2579 1676 1461 669 414 334 258 92
30
Bpu10I CC'TnA_GC
Cuts at: 3434 3773 4478 3434
Size: 339 705 6469
Fragments arranged by size:
6469 705 339
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2516 5638 6503 6801 2516
Size: 3122 865 298 3228
Fragments arranged by size:
3228 3122 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2695 3047 3395 6176 882
Size: 617 1196 352 348 2781 2219
Fragments arranged by size:
2781 2219 1196 617 352 348
BsaAI yAC'GTr
Cuts at: 493 1204 3071 3547 5304 493
Size: 711 1867 476 1757 2702
Fragments arranged by size:
2702 1867 1757 711 476
BsaBI GATnn'nnATC
Cuts at: 2582 7507 2582
Size: 4925 2588
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2026 2824 5320 5702
Size: 53 83 186 936 493 798 2496 382
Cuts at: 5702 6337 275
Size: 635 1451
Fragments arranged by size:
2496 1451 936 798 635 493 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2082 2943 4684 5946 6344 6618 6765 7176 2082
Size: 861 1741 1262 398 274 147 411 2419
Fragments arranged by size:
2419 1741 1262 861 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2165 2195 3443 3473 3677
Size: 30 165 30 762 30 1248 30 204
Cuts at: 3677 3707 3802 3832 4241 4271 1178
Size: 30 95 30 409 30 4420
Fragments arranged by size:
4420 1248 762 409 204 165 95 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2622 2930 3911 4006 4646 4703 1186
Size: 1436 308 981 95 640 57 3996
Fragments arranged by size:
3996 1436 981 640 308 95 57
BseYI C'CCAG_C
Cuts at: 2361 3139 3328 3926 4152 4162 6716 7212 2361
Size: 778 189 598 226 10 2554 496 2662
Fragments arranged by size:
2662 2554 778 598 496 226 189 10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2107 2321 2580 2792 3640 3700 4442
Size: 573 371 214 259 212 848 60 742
Cuts at: 4442 1163
Size: 4234
Fragments arranged by size:
4234 848 742 573 371 259 214 212
60
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2145 3115 4963 5724 6752 1540
Size: 275 186 144 970 1848 761 1028 2301
Fragments arranged by size:
2301 1848 1028 970 761 275 186 144
BsiHKAI G_wGCw'C
Cuts at: 3123 3315 3546 3903 3915 4029 5574 5659 6730
Size: 192 231 357 12 114 1545 85 1071
Cuts at: 6730 3123
Size: 3906
Fragments arranged by size:
3906 1545 1071 357 231 192 114 85
12
BsmI GAATG_Cn'
Cuts at: 5024 5117 5024
Size: 93 7420
BsmBI CGTCTCn'nnnn_
Cuts at: 2168 2168
Size: 7513
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2154 2213 2519 2848 3123 3315 3488
Size: 399 364 59 306 329 275 192 173
Cuts at: 3488 3546 3815 3842 3884 3903 3915 3953 4029
Size: 58 269 27 42 19 12 38 76
Cuts at: 4029 4038 4065 4152 4215 4232 4254 4471 4642
Size: 9 27 87 63 17 22 217 171
Cuts at: 4642 4663 4726 4832 5574 5659 6730 1391
Size: 21 63 106 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 364 329 306 275
269 217 192 173 171 106 87 85
76 63 63 59 58 42 38 27
27 22 21 19 17 12 9
BspEI T'CCGG_A
Cuts at: 4684 7176 4684
Size: 2492 5021
BspHI T'CATG_A
Cuts at: 5402 7132 5402
Size: 1730 5783
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2999 3871 3992 4351 4868 844
Size: 2155 872 121 359 517 3489
Fragments arranged by size:
3489 2155 872 517 359 121
BsrBI CCG'CTC
Cuts at: 1394 1823 2530 3293 3510 3573 3612 4636 5400
Size: 429 707 763 217 63 39 1024 764
Cuts at: 5400 1394
Size: 3507
Fragments arranged by size:
3507 1024 764 763 707 429 217 63
39
BsrDI GCAATG_nn'
Cuts at: 65 1248 6008 6182 65
Size: 1183 4760 174 1396
Fragments arranged by size:
4760 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2240 2539 2943 3097 3204 6157 6344
Size: 110 430 299 404 154 107 2953 187
Cuts at: 6344 1700
Size: 2869
Fragments arranged by size:
2953 2869 430 404 299 187 154 110
107
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7513
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7513
BssSI C'ACGA_G
Cuts at: 4118 5573 6585 4118
Size: 1455 1012 5046
Fragments arranged by size:
5046 1455 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4392 794
Size: 3598 3915
BstBI TT'CG_AA
Cuts at: 6317 6317
Size: 7513
BstEII G'GTnAC_C
Cuts at: 1184 3088 1184
Size: 1904 5609
BstXI CCAn_nnnn'nTGG
Cuts at: 2893 2893
Size: 7513
BstYI r'GATC_y
Cuts at: 1972 2616 4778 4843 5210 5595 5612 7064 7150
Size: 644 2162 65 367 385 17 1452 86
Cuts at: 7150 7162 7508 1972
Size: 12 346 1977
Fragments arranged by size:
2162 1977 1452 644 385 367 346 86
65 17 12
Bsu36I CC'TnA_GG
Cuts at: 4094 4094
Size: 7513
BtgI C'CryG_G
Cuts at: 513 1065 2037 2074 2145 3172 3198 3537 4369
Size: 552 972 37 71 1027 26 339 832
Cuts at: 4369 4595 513
Size: 226 3431
Fragments arranged by size:
3431 1027 972 832 552 339 226 71
37 26
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6774
Fragments arranged by size:
6774 683 56
BtsI GCAGTG_nn'
Cuts at: 3520 3628 4752 5029 5823 5843 3520
Size: 108 1124 277 794 20 5190
Fragments arranged by size:
5190 1124 794 277 108 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7513
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3340 3375 529
Size: 35 2776 35 4667
Fragments arranged by size:
4667 2776 35 35
DraI TTT'AAA
Cuts at: 4817 4817
Size: 7513
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6520 809
Size: 507 5204 1802
Fragments arranged by size:
5204 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2142 3112 3288
Size: 54 1425 50 275 18 312 970 176
Cuts at: 3288 3361 3604 3633 3676 3944 4960 5849 7478
Size: 73 243 29 43 268 1016 889 1629
Cuts at: 7478 8
Size: 43
Fragments arranged by size:
1629 1425 1016 970 889 312 275 268
243 176 73 54 50 43 43 29
18
EagI C'GGCC_G
Cuts at: 1537 1812 2142 3112 4960 1537
Size: 275 330 970 1848 4090
Fragments arranged by size:
4090 1848 970 330 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2334 3533 4347 4623 5443 1353
Size: 981 1199 814 276 820 3423
Fragments arranged by size:
3423 1199 981 820 814 276
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4392 6100 6474 6620 387
Size: 1064 138 2803 1708 374 146 1280
Fragments arranged by size:
2803 1708 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3901 3901
Size: 7513
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2541 2750 2915 3329 3574 3587 3679
Size: 393 909 209 165 414 245 13 92
Cuts at: 3679 4013 4043 4712 5576 6173 6960 7228 1239
Size: 334 30 669 864 597 787 268 1524
Fragments arranged by size:
1524 909 864 787 669 597 414 393
334 268 245 209 165 92 30 13
EcoNI CCTnn'n_nnAGG
Cuts at: 2426 3343 4223 2426
Size: 917 880 5716
Fragments arranged by size:
5716 917 880
EcoO109I rG'GnC_Cy
Cuts at: 2150 2844 2845 3430 3484 3860 3950 4034 4101
Size: 694 1 585 54 376 90 84 67
Cuts at: 4101 4211 4212 4467 4474 4548 4609 4659 4660
Size: 110 1 255 7 74 61 50 1
Cuts at: 4660 2150
Size: 5003
Fragments arranged by size:
5003 694 585 376 255 110 90 84
74 67 61 54 50 7 1 1
1
EcoRI G'AATT_C
Cuts at: 2749 4822 5200 2749
Size: 2073 378 5062
Fragments arranged by size:
5062 2073 378
FspI TGC'GCA
Cuts at: 6019 6019
Size: 7513
HaeII r_GCGC'y
Cuts at: 1678 2029 2286 2827 4179 6660 1678
Size: 351 257 541 1352 2481 2531
Fragments arranged by size:
2531 2481 1352 541 351 257
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2601 2633 3443 3475 3802
Size: 32 135 32 1029 32 810 32 327
Cuts at: 3802 3834 3962 3994 4112 4144 4241 4273 6154
Size: 32 128 32 118 32 97 32 1881
Cuts at: 6154 6186 6228 6260 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1881 1029 810 327 135 128 118
97 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2590 4857 5103 669
Size: 988 281 652 2267 246 3079
Fragments arranged by size:
3079 2267 988 652 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7513
HpaI GTT'AAC
Cuts at: 5103 5103
Size: 7513
KasI G'GCGC_C
Cuts at: 2025 2823 2025
Size: 798 6715
KpnI G_GTAC'C
Cuts at: 4838 4838
Size: 7513
MfeI C'AATT_G
Cuts at: 5112 5112
Size: 7513
MluI A'CGCG_T
Cuts at: 7389 7389
Size: 7513
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2901 6627 6811 1305
Size: 1596 3726 184 2007
Fragments arranged by size:
3726 2007 1596 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5691
Fragments arranged by size:
5691 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3539 3918 5471 5830 5989 518
Size: 648 2373 379 1553 359 159 2042
Fragments arranged by size:
2373 2042 1553 648 379 359 159
MspA1I CmG'CkG
Cuts at: 1606 2076 2113 2119 2147 2449 2506 3300 3594
Size: 470 37 6 28 302 57 794 294
Cuts at: 3594 4187 4371 4389 4929 5606 6754 6999 1606
Size: 593 184 18 540 677 1148 245 2120
Fragments arranged by size:
2120 1148 794 677 593 540 470 302
294 245 184 57 37 28 18 6
NarI GG'CG_CC
Cuts at: 2026 2824 2026
Size: 798 6715
NcoI C'CATG_G
Cuts at: 513 1065 3198 513
Size: 552 2133 4828
Fragments arranged by size:
4828 2133 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7513
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7513
NotI GC'GGCC_GC
Cuts at: 2142 4960 2142
Size: 2818 4695
NspI r_CATG'y
Cuts at: 1132 1229 4871 6416 1132
Size: 97 3642 1545 2229
Fragments arranged by size:
3642 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2030 4270 1348
Size: 682 2240 4591
Fragments arranged by size:
4591 2240 682
PciI A'CATG_T
Cuts at: 6412 6412
Size: 7513
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3286 1108
Size: 2178 5335
PfoI T'CCnGG_A
Cuts at: 4728 4728
Size: 7513
PmeI GTTT'AAAC
Cuts at: 4817 4817
Size: 7513
PmlI CAC'GTG
Cuts at: 3071 3547 3071
Size: 476 7037
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2165 2197 2501 2533 5582 5614 7095 7127 2165
Size: 32 304 32 3049 32 1481 32 2551
Fragments arranged by size:
3049 2551 1481 304 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3860 4101 4474 4548 3860
Size: 241 373 74 6825
Fragments arranged by size:
6825 373 241 74
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7513
PsiI TTA'TAA
Cuts at: 5083 5083
Size: 7513
PspOMI G'GGCC_C
Cuts at: 2150 2844 3484 3949 4034 4211 4467 4659 2150
Size: 694 640 465 85 177 256 192 5004
Fragments arranged by size:
5004 694 640 465 256 192 177 85
PspXI vC'TCGA_Gb
Cuts at: 2607 4064 2607
Size: 1457 6056
PstI C_TGCA'G
Cuts at: 830 2464 2982 3989 4771 4865 830
Size: 1634 518 1007 782 94 3478
Fragments arranged by size:
3478 1634 1007 782 518 94
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7513
PvuII CAG'CTG
Cuts at: 2506 3594 4187 2506
Size: 1088 593 5832
Fragments arranged by size:
5832 1088 593
SacI G_AGCT'C
Cuts at: 3903 3903
Size: 7513
SacII CC_GC'GG
Cuts at: 2077 2148 4372 2077
Size: 71 2224 5218
Fragments arranged by size:
5218 2224 71
SalI G'TCGA_C
Cuts at: 1936 4855 1936
Size: 2919 4594
SanDI GG'GwC_CC
Cuts at: 4548 4548
Size: 7513
SapI GCTCTTCn'nnn_
Cuts at: 3533 3533
Size: 7513
SbfI CC_TGCA'GG
Cuts at: 4865 4865
Size: 7513
ScaI AGT'ACT
Cuts at: 5761 7473 5761
Size: 1712 5801
SfcI C'TryA_G
Cuts at: 826 1045 2460 2978 3985 4003 4767 4861 5996
Size: 219 1415 518 1007 18 764 94 1135
Cuts at: 5996 6677 6868 826
Size: 681 191 1471
Fragments arranged by size:
1471 1415 1135 1007 764 681 518 219
191 94 18
SfiI GGCCn_nnn'nGGCC
Cuts at: 2250 2250
Size: 7513
SfoI GGC'GCC
Cuts at: 2027 2825 2027
Size: 798 6715
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7513
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7513
SmaI CCC'GGG
Cuts at: 1872 3482 4465 4472 4625 4806 4840 1872
Size: 1610 983 7 153 181 34 4545
Fragments arranged by size:
4545 1610 983 181 153 34 7
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2531 2607 4064 5617 6518 6780
Size: 197 926 588 76 1457 1553 901 262
Cuts at: 6780 820
Size: 1553
Fragments arranged by size:
1553 1553 1457 926 901 588 262 197
76
SnaBI TAC'GTA
Cuts at: 493 5304 493
Size: 4811 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7513
SphI G_CATG'C
Cuts at: 1132 4871 1132
Size: 3739 3774
SspI AAT'ATT
Cuts at: 5 52 5437 5
Size: 47 5385 2081
Fragments arranged by size:
5385 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7513
StyI C'CwwG_G
Cuts at: 513 1065 2169 2422 2694 3198 3375 3941 4543
Size: 552 1104 253 272 504 177 566 602
Cuts at: 4543 4952 513
Size: 409 3074
Fragments arranged by size:
3074 1104 602 566 552 504 409 272
253 177
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2325 4789 5540 5557 5710 5895 6340
Size: 1255 745 2464 751 17 153 185 445
Cuts at: 6340 325
Size: 1498
Fragments arranged by size:
2464 1498 1255 751 745 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2443 2901 2936
Size: 274 80 33 51 1443 466 458 35
Cuts at: 2936 4425 5759 7471 96
Size: 1489 1334 1712 138
Fragments arranged by size:
1712 1489 1443 1334 466 458 274 138
80 51 35 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3677 3709 7473 7505 3677
Size: 32 3764 32 3685
Fragments arranged by size:
3764 3685 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2742 1316
Size: 1426 6087
XbaI T'CTAG_A
Cuts at: 2779 4849 2779
Size: 2070 5443
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2761 3703 3988 2761
Size: 942 285 6286
Fragments arranged by size:
6286 942 285
XhoI C'TCGA_G
Cuts at: 1943 2607 4064 1943
Size: 664 1457 5392
Fragments arranged by size:
5392 1457 664
XmaI C'CCGG_G
Cuts at: 1870 3480 4463 4470 4623 4804 4838 1870
Size: 1610 983 7 153 181 34 4545
Fragments arranged by size:
4545 1610 983 181 153 34 7
XmnI GAAnn'nnTTC
Cuts at: 5642 5642
Size: 7513
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5321 276
Size: 53 83 186 936 3787 2468
Fragments arranged by size:
3787 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI
PvuII SacI SacII SalI SanDI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI BaeI BfrBI BsiWI BstZ17I DraIII EcoRV
FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI
PsrI RsrII SexAI SrfI SwaI