Restriction map for
pFN21AE2780
(Circular) (Six-base) MAPSORT of: pFN21AE2780.seq Check: 879 from: 1 to: 7129
pFN21AE2780 7129 bp
With 182 enzymes: SgfI *
July 4, 2020 18:17 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4939 278
Size: 53 83 186 936 3403 2468
Fragments arranged by size:
3403 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4472 1937
Size: 2535 4594
Acc65I G'GTAC_C
Cuts at: 2705 4450 2705
Size: 1745 5384
AclI AA'CG_TT
Cuts at: 1563 5256 5629 1563
Size: 3693 373 3063
Fragments arranged by size:
3693 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2158 4055 4110 4188 4352 5192 6576 6844
Size: 526 1897 55 78 164 840 1384 268
Cuts at: 6844 1632
Size: 1917
Fragments arranged by size:
1917 1897 1384 840 526 268 164 78
55
AfeI AGC'GCT
Cuts at: 2348 3527 2348
Size: 1179 5950
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6932
AflIII A'CryG_T
Cuts at: 1203 6028 7005 1203
Size: 4825 977 1327
Fragments arranged by size:
4825 1327 977
AgeI A'CCGG_T
Cuts at: 5960 5960
Size: 7129
AhdI GACnn_n'nnGTC
Cuts at: 5858 5858
Size: 7129
AleI CACnn'nnGTG
Cuts at: 1166 4358 1166
Size: 3192 3937
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7129
AlwNI CAG_nnn'CTG
Cuts at: 4277 4872 6444 4277
Size: 595 1572 4962
Fragments arranged by size:
4962 1572 595
ApaLI G'TGCA_C
Cuts at: 5186 6342 5186
Size: 1156 5973
ApoI r'AATT_y
Cuts at: 1493 3412 3654 4133 4438 4664 4816 6925 1493
Size: 1919 242 479 305 226 152 2109 1697
Fragments arranged by size:
2109 1919 1697 479 305 242 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2514 3284 3944 5683 160
Size: 872 1482 770 660 1739 1606
Fragments arranged by size:
1739 1606 1482 872 770 660
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7129
AvaI C'yCGr_G
Cuts at: 1870 1943 4454 1870
Size: 73 2511 4545
Fragments arranged by size:
4545 2511 73
BamHI G'GATC_C
Cuts at: 2393 4459 4826 2393
Size: 2066 367 4696
Fragments arranged by size:
4696 2066 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2156 2705 3223 4450 5905
Size: 325 217 627 369 549 518 1227 1455
Cuts at: 5905 618
Size: 1842
Fragments arranged by size:
1842 1455 1227 627 549 518 369 325
217
BanII G_rGCy'C
Cuts at: 1391 3576 4448 1391
Size: 2185 872 4072
Fragments arranged by size:
4072 2185 872
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3373 4061 4356 4408 928
Size: 971 1474 688 295 52 3649
Fragments arranged by size:
3649 1474 971 688 295 52
BbvCI CC'TCA_GC
Cuts at: 3542 3542
Size: 7129
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5320 5354 1903
Size: 34 3383 34 3678
Fragments arranged by size:
3678 3383 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5021 6231 5021
Size: 1210 5919
BclI T'GATC_A
Cuts at: 1548 3088 3916 1548
Size: 1540 828 4761
Fragments arranged by size:
4761 1540 828
BfrBI ATG'CAT
Cuts at: 3239 3420 3239
Size: 181 6948
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5740 136
Size: 107 122 71 631 605 4068 1525
Fragments arranged by size:
4068 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2085 7124 2085
Size: 5039 2090
BlpI GC'TnA_GC
Cuts at: 4546 4546
Size: 7129
Bme1580I G_kGCm'C
Cuts at: 1790 2140 2159 5190 6346 1790
Size: 350 19 3031 1156 2573
Fragments arranged by size:
3031 2573 1156 350 19
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7129
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4860 5818 442
Size: 416 53 83 399 24 3443 958 1753
Fragments arranged by size:
3443 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7129
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2915 3609 4326 5789 1239
Size: 1676 694 717 1463 2579
Fragments arranged by size:
2579 1676 1463 717 694
Bpu10I CC'TnA_GC
Cuts at: 3542 3542
Size: 7129
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5254 6119 6417 5254
Size: 865 298 5966
Fragments arranged by size:
5966 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5792 882
Size: 617 4293 2219
Fragments arranged by size:
4293 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4920 493
Size: 711 3716 2702
Fragments arranged by size:
3716 2702 711
BsaBI GATnn'nnATC
Cuts at: 7123 7123
Size: 7129
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2358 4936 5318 5953
Size: 53 83 186 936 825 2578 382 635
Cuts at: 5953 275
Size: 1451
Fragments arranged by size:
2578 1451 936 825 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5562 5960 6234 6381 6792 5562
Size: 398 274 147 411 5899
Fragments arranged by size:
5899 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2871 2901 2946 2976 3436
Size: 30 165 30 1468 30 45 30 460
Cuts at: 3436 3466 4212 4242 1178
Size: 30 746 30 4065
Fragments arranged by size:
4065 1468 746 460 165 45 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2390 2970 3875 1186
Size: 1204 580 905 4440
Fragments arranged by size:
4440 1204 905 580
BseYI C'CCAG_C
Cuts at: 2281 6332 6828 2281
Size: 4051 496 2582
Fragments arranged by size:
4051 2582 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6556
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4579 5340 6368 1540
Size: 275 186 2578 761 1028 2301
Fragments arranged by size:
2578 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2632 3576 5190 5275 6346 2632
Size: 944 1614 85 1071 3415
Fragments arranged by size:
3415 1614 1071 944 85
BsmI GAATG_Cn'
Cuts at: 2497 4640 4733 2497
Size: 2143 93 4893
Fragments arranged by size:
4893 2143 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2793 2963 2793
Size: 170 6959
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2140 2159 2632 3576 4448 5190 5275
Size: 399 350 19 473 944 872 742 85
Cuts at: 5275 6346 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 944 872 742 473 399 350
85 19
BspEI T'CCGG_A
Cuts at: 6792 6792
Size: 7129
BspHI T'CATG_A
Cuts at: 3846 5018 6748 3846
Size: 1172 1730 4227
Fragments arranged by size:
4227 1730 1172
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4484 844
Size: 3640 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 5016 1394
Size: 429 3193 3507
Fragments arranged by size:
3507 3193 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5624 5798 65
Size: 1183 4376 174 1396
Fragments arranged by size:
4376 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5773 5960 1700
Size: 110 3963 187 2869
Fragments arranged by size:
3963 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7129
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7129
BssSI C'ACGA_G
Cuts at: 2853 4251 5189 6201 2853
Size: 1398 938 1012 3781
Fragments arranged by size:
3781 1398 1012 938
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7129
BstBI TT'CG_AA
Cuts at: 5933 5933
Size: 7129
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7129
BstXI CCAn_nnnn'nTGG
Cuts at: 2309 4036 2309
Size: 1727 5402
BstYI r'GATC_y
Cuts at: 1972 2085 2393 3160 4394 4459 4826 5211 5228
Size: 113 308 767 1234 65 367 385 17
Cuts at: 5228 6680 6766 6778 7124 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1234 767 385 367 346 308
113 86 65 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2641 4177 513
Size: 552 1576 1536 3465
Fragments arranged by size:
3465 1576 1536 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6390
Fragments arranged by size:
6390 683 56
BtsI GCAGTG_nn'
Cuts at: 4645 5439 5459 4645
Size: 794 20 6315
Fragments arranged by size:
6315 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7129
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7094
DraI TTT'AAA
Cuts at: 3759 4433 3759
Size: 674 6455
DraIII CAC_nnn'GTG
Cuts at: 2858 2858
Size: 7129
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6136 809
Size: 507 4820 1802
Fragments arranged by size:
4820 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4576 5465 7094
Size: 54 1425 50 275 18 2746 889 1629
Cuts at: 7094 8
Size: 43
Fragments arranged by size:
2746 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4576 1537
Size: 275 2764 4090
Fragments arranged by size:
4090 2764 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2791 3684 5059 1353
Size: 1438 893 1375 3423
Fragments arranged by size:
3423 1438 1375 893
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5716 6090 6236 387
Size: 1064 138 4127 374 146 1280
Fragments arranged by size:
4127 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3574 3574
Size: 7129
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2158 2915 3609 4055 4110 4188 4326
Size: 393 526 757 694 446 55 78 138
Cuts at: 4326 4352 5192 5789 6576 6844 1239
Size: 26 840 597 787 268 1524
Fragments arranged by size:
1524 840 787 757 694 597 526 446
393 268 138 78 55 26
EcoO109I rG'GnC_Cy
Cuts at: 2788 3094 4094 4274 2788
Size: 306 1000 180 5643
Fragments arranged by size:
5643 1000 306 180
EcoRI G'AATT_C
Cuts at: 4133 4438 4816 4133
Size: 305 378 6446
Fragments arranged by size:
6446 378 305
EcoRV GAT'ATC
Cuts at: 2449 2449
Size: 7129
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2188 2413 2759 3338 4085 2188
Size: 225 346 579 747 5232
Fragments arranged by size:
5232 747 579 346 225
FspI TGC'GCA
Cuts at: 5635 5635
Size: 7129
HaeII r_GCGC'y
Cuts at: 1678 2350 3274 3529 6276 1678
Size: 672 924 255 2747 2531
Fragments arranged by size:
2747 2531 924 672 255
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2077 2109 2732 2764 2946
Size: 32 135 32 505 32 623 32 182
Cuts at: 2946 2978 5770 5802 5844 5876 1373
Size: 32 2792 32 42 32 2626
Fragments arranged by size:
2792 2626 623 505 182 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2053 2529 4473 4719 669
Size: 988 281 115 476 1944 246 3079
Fragments arranged by size:
3079 1944 988 476 281 246 115
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7129
HpaI GTT'AAC
Cuts at: 2053 4719 2053
Size: 2666 4463
KpnI G_GTAC'C
Cuts at: 2709 4454 2709
Size: 1745 5384
MfeI C'AATT_G
Cuts at: 2988 4728 2988
Size: 1740 5389
MluI A'CGCG_T
Cuts at: 7005 7005
Size: 7129
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3889 6243 6427 1305
Size: 2584 2354 184 2007
Fragments arranged by size:
2584 2354 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5307
Fragments arranged by size:
5307 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2307 4358 5087 5446 5605 518
Size: 648 1141 2051 729 359 159 2042
Fragments arranged by size:
2051 2042 1141 729 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2762 3398 4545 5222 6370 6615 1606
Size: 1156 636 1147 677 1148 245 2120
Fragments arranged by size:
2120 1156 1148 1147 677 636 245
NcoI C'CATG_G
Cuts at: 513 1065 2641 4177 513
Size: 552 1576 1536 3465
Fragments arranged by size:
3465 1576 1536 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7129
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7129
NotI GC'GGCC_GC
Cuts at: 4576 4576
Size: 7129
NsiI A_TGCA'T
Cuts at: 3241 3422 3241
Size: 181 6948
NspI r_CATG'y
Cuts at: 1132 1229 4487 6032 1132
Size: 97 3258 1545 2229
Fragments arranged by size:
3258 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7129
PciI A'CATG_T
Cuts at: 6028 6028
Size: 7129
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7129
PmeI GTTT'AAAC
Cuts at: 4433 4433
Size: 7129
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5198 5230 6711 6743 5198
Size: 32 1481 32 5584
Fragments arranged by size:
5584 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3094 4094 4274 3094
Size: 1000 180 5949
Fragments arranged by size:
5949 1000 180
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7129
PsiI TTA'TAA
Cuts at: 4025 4699 4025
Size: 674 6455
PstI C_TGCA'G
Cuts at: 830 3294 3562 4481 830
Size: 2464 268 919 3478
Fragments arranged by size:
3478 2464 919 268
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7129
PvuII CAG'CTG
Cuts at: 2762 3398 2762
Size: 636 6493
SacI G_AGCT'C
Cuts at: 3576 3576
Size: 7129
SalI G'TCGA_C
Cuts at: 1936 4471 1936
Size: 2535 4594
SapI GCTCTTCn'nnn_
Cuts at: 3684 3684
Size: 7129
SbfI CC_TGCA'GG
Cuts at: 4481 4481
Size: 7129
ScaI AGT'ACT
Cuts at: 5377 7089 5377
Size: 1712 5417
SexAI A'CCwGG_T
Cuts at: 3739 3739
Size: 7129
SfcI C'TryA_G
Cuts at: 826 1045 3290 3558 4477 5612 6293 6484 826
Size: 219 2245 268 919 1135 681 191 1471
Fragments arranged by size:
2245 1471 1135 919 681 268 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7129
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7129
SmaI CCC'GGG
Cuts at: 1872 4456 1872
Size: 2584 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5233 6134 6396 820
Size: 197 926 3290 901 262 1553
Fragments arranged by size:
3290 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4920 493
Size: 4427 2702
SpeI A'CTAG_T
Cuts at: 152 3638 152
Size: 3486 3643
SphI G_CATG'C
Cuts at: 1132 4487 1132
Size: 3355 3774
SspI AAT'ATT
Cuts at: 5 52 5053 5
Size: 47 5001 2081
Fragments arranged by size:
5001 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7129
StyI C'CwwG_G
Cuts at: 513 1065 2119 2532 2641 2791 4177 4568 513
Size: 552 1054 413 109 150 1386 391 3074
Fragments arranged by size:
3074 1386 1054 552 413 391 150 109
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5156 5173 5326 5511 5956 325
Size: 1255 3576 17 153 185 445 1498
Fragments arranged by size:
3576 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5375 7087 96
Size: 274 80 33 51 1443 3398 1712 138
Fragments arranged by size:
3398 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2844 2871 2876 2903 3436 3468 4212 4244 7089
Size: 27 5 27 533 32 744 32 2845
Cuts at: 7089 7121 2844
Size: 32 2852
Fragments arranged by size:
2852 2845 744 533 32 32 32 27
27 5
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7129
XbaI T'CTAG_A
Cuts at: 4397 4465 4397
Size: 68 7061
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2603 2603
Size: 7129
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7129
XmaI C'CCGG_G
Cuts at: 1870 4454 1870
Size: 2584 4545
XmnI GAAnn'nnTTC
Cuts at: 2171 5258 2171
Size: 3087 4042
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4937 276
Size: 53 83 186 936 3403 2468
Fragments arranged by size:
3403 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SacI SalI SapI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BplI
BsiWI BstZ17I Bsu36I EcoNI FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI
PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI