Restriction map for
pFN21AE2779
(Circular) (Six-base) MAPSORT of: pFN21AE2779.seq Check: 5602 from: 1 to: 7132
pFN21AE2779 7132 bp
With 182 enzymes: SgfI *
July 4, 2020 18:17 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4942 278
Size: 53 83 186 936 3406 2468
Fragments arranged by size:
3406 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4475 1937
Size: 2538 4594
Acc65I G'GTAC_C
Cuts at: 2705 4453 2705
Size: 1748 5384
AclI AA'CG_TT
Cuts at: 1563 5259 5632 1563
Size: 3696 373 3063
Fragments arranged by size:
3696 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2158 4107 4271 4331 5195 6579 6847 1632
Size: 526 1949 164 60 864 1384 268 1917
Fragments arranged by size:
1949 1917 1384 864 526 268 164 60
AfeI AGC'GCT
Cuts at: 2348 3527 2348
Size: 1179 5953
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6935
AflIII A'CryG_T
Cuts at: 1203 6031 7008 1203
Size: 4828 977 1327
Fragments arranged by size:
4828 1327 977
AgeI A'CCGG_T
Cuts at: 5963 5963
Size: 7132
AhdI GACnn_n'nnGTC
Cuts at: 5861 5861
Size: 7132
AleI CACnn'nnGTG
Cuts at: 1166 4361 1166
Size: 3195 3937
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7132
AlwNI CAG_nnn'CTG
Cuts at: 4196 4875 6447 4196
Size: 679 1572 4881
Fragments arranged by size:
4881 1572 679
ApaLI G'TGCA_C
Cuts at: 5189 6345 5189
Size: 1156 5976
ApoI r'AATT_y
Cuts at: 1493 3412 3654 4052 4289 4441 4667 4819 6928
Size: 1919 242 398 237 152 226 152 2109
Cuts at: 6928 1493
Size: 1697
Fragments arranged by size:
2109 1919 1697 398 242 237 226 152
152
AseI AT'TA_AT
Cuts at: 160 1032 2514 3284 3944 5686 160
Size: 872 1482 770 660 1742 1606
Fragments arranged by size:
1742 1606 1482 872 770 660
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7132
AvaI C'yCGr_G
Cuts at: 1870 1943 4457 1870
Size: 73 2514 4545
Fragments arranged by size:
4545 2514 73
BamHI G'GATC_C
Cuts at: 2393 4462 4829 2393
Size: 2069 367 4696
Fragments arranged by size:
4696 2069 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2156 2705 3223 4453 5908
Size: 325 217 627 369 549 518 1230 1455
Cuts at: 5908 618
Size: 1842
Fragments arranged by size:
1842 1455 1230 627 549 518 369 325
217
BanII G_rGCy'C
Cuts at: 1391 3576 4451 1391
Size: 2185 875 4072
Fragments arranged by size:
4072 2185 875
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3373 4411 928
Size: 971 1474 1038 3649
Fragments arranged by size:
3649 1474 1038 971
BbvCI CC'TCA_GC
Cuts at: 3542 3542
Size: 7132
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5323 5357 1903
Size: 34 3386 34 3678
Fragments arranged by size:
3678 3386 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5024 6234 5024
Size: 1210 5922
BclI T'GATC_A
Cuts at: 1548 3088 3916 1548
Size: 1540 828 4764
Fragments arranged by size:
4764 1540 828
BfrBI ATG'CAT
Cuts at: 3239 3420 3239
Size: 181 6951
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5743 136
Size: 107 122 71 631 605 4071 1525
Fragments arranged by size:
4071 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2085 7127 2085
Size: 5042 2090
BlpI GC'TnA_GC
Cuts at: 4549 4549
Size: 7132
Bme1580I G_kGCm'C
Cuts at: 1790 2140 2159 5193 6349 1790
Size: 350 19 3034 1156 2573
Fragments arranged by size:
3034 2573 1156 350 19
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7132
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4863 5821 442
Size: 416 53 83 399 24 3446 958 1753
Fragments arranged by size:
3446 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7132
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2915 3609 4245 5792 1239
Size: 1676 694 636 1547 2579
Fragments arranged by size:
2579 1676 1547 694 636
Bpu10I CC'TnA_GC
Cuts at: 3542 3542
Size: 7132
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5257 6122 6420 5257
Size: 865 298 5969
Fragments arranged by size:
5969 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5795 882
Size: 617 4296 2219
Fragments arranged by size:
4296 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4923 493
Size: 711 3719 2702
Fragments arranged by size:
3719 2702 711
BsaBI GATnn'nnATC
Cuts at: 7126 7126
Size: 7132
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2358 4939 5321 5956
Size: 53 83 186 936 825 2581 382 635
Cuts at: 5956 275
Size: 1451
Fragments arranged by size:
2581 1451 936 825 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5565 5963 6237 6384 6795 5565
Size: 398 274 147 411 5902
Fragments arranged by size:
5902 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2871 2901 2946 2976 3436
Size: 30 165 30 1468 30 45 30 460
Cuts at: 3436 3466 4131 4161 1178
Size: 30 665 30 4149
Fragments arranged by size:
4149 1468 665 460 165 45 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2390 2970 3875 1186
Size: 1204 580 905 4443
Fragments arranged by size:
4443 1204 905 580
BseYI C'CCAG_C
Cuts at: 2281 6335 6831 2281
Size: 4054 496 2582
Fragments arranged by size:
4054 2582 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6559
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4582 5343 6371 1540
Size: 275 186 2581 761 1028 2301
Fragments arranged by size:
2581 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2632 3576 5193 5278 6349 2632
Size: 944 1617 85 1071 3415
Fragments arranged by size:
3415 1617 1071 944 85
BsmI GAATG_Cn'
Cuts at: 2497 4643 4736 2497
Size: 2146 93 4893
Fragments arranged by size:
4893 2146 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2793 2963 2793
Size: 170 6962
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2140 2159 2632 3576 4451 5193 5278
Size: 399 350 19 473 944 875 742 85
Cuts at: 5278 6349 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 944 875 742 473 399 350
85 19
BspEI T'CCGG_A
Cuts at: 6795 6795
Size: 7132
BspHI T'CATG_A
Cuts at: 3846 5021 6751 3846
Size: 1175 1730 4227
Fragments arranged by size:
4227 1730 1175
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4487 844
Size: 3643 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 5019 1394
Size: 429 3196 3507
Fragments arranged by size:
3507 3196 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5627 5801 65
Size: 1183 4379 174 1396
Fragments arranged by size:
4379 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5776 5963 1700
Size: 110 3966 187 2869
Fragments arranged by size:
3966 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7132
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7132
BssSI C'ACGA_G
Cuts at: 2853 4170 5192 6204 2853
Size: 1317 1022 1012 3781
Fragments arranged by size:
3781 1317 1022 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7132
BstBI TT'CG_AA
Cuts at: 5936 5936
Size: 7132
BstEII G'GTnAC_C
Cuts at: 1184 4273 4282 1184
Size: 3089 9 4034
Fragments arranged by size:
4034 3089 9
BstXI CCAn_nnnn'nTGG
Cuts at: 2309 2309
Size: 7132
BstYI r'GATC_y
Cuts at: 1972 2085 2393 3160 4397 4462 4829 5214 5231
Size: 113 308 767 1237 65 367 385 17
Cuts at: 5231 6683 6769 6781 7127 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1237 767 385 367 346 308
113 86 65 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2641 4096 513
Size: 552 1576 1455 3549
Fragments arranged by size:
3549 1576 1455 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6393
Fragments arranged by size:
6393 683 56
BtsI GCAGTG_nn'
Cuts at: 4648 5442 5462 4648
Size: 794 20 6318
Fragments arranged by size:
6318 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7132
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7097
DraI TTT'AAA
Cuts at: 3759 4436 3759
Size: 677 6455
DraIII CAC_nnn'GTG
Cuts at: 2858 2858
Size: 7132
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6139 809
Size: 507 4823 1802
Fragments arranged by size:
4823 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4579 5468 7097
Size: 54 1425 50 275 18 2749 889 1629
Cuts at: 7097 8
Size: 43
Fragments arranged by size:
2749 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4579 1537
Size: 275 2767 4090
Fragments arranged by size:
4090 2767 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2791 3684 4263 5062 1353
Size: 1438 893 579 799 3423
Fragments arranged by size:
3423 1438 893 799 579
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5719 6093 6239 387
Size: 1064 138 4130 374 146 1280
Fragments arranged by size:
4130 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3574 3574
Size: 7132
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2158 2915 3609 4107 4245 4271 4331
Size: 393 526 757 694 498 138 26 60
Cuts at: 4331 5195 5792 6579 6847 1239
Size: 864 597 787 268 1524
Fragments arranged by size:
1524 864 787 757 694 597 526 498
393 268 138 60 26
EcoO109I rG'GnC_Cy
Cuts at: 2788 3094 4193 2788
Size: 306 1099 5727
Fragments arranged by size:
5727 1099 306
EcoRI G'AATT_C
Cuts at: 4052 4441 4819 4052
Size: 389 378 6365
Fragments arranged by size:
6365 389 378
EcoRV GAT'ATC
Cuts at: 2449 2449
Size: 7132
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2188 2413 2759 3338 2188
Size: 225 346 579 5982
Fragments arranged by size:
5982 579 346 225
FspI TGC'GCA
Cuts at: 5638 5638
Size: 7132
HaeII r_GCGC'y
Cuts at: 1678 2350 3274 3529 6279 1678
Size: 672 924 255 2750 2531
Fragments arranged by size:
2750 2531 924 672 255
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2077 2109 2732 2764 2946
Size: 32 135 32 505 32 623 32 182
Cuts at: 2946 2978 5773 5805 5847 5879 1373
Size: 32 2795 32 42 32 2626
Fragments arranged by size:
2795 2626 623 505 182 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2053 2529 4476 4722 669
Size: 988 281 115 476 1947 246 3079
Fragments arranged by size:
3079 1947 988 476 281 246 115
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7132
HpaI GTT'AAC
Cuts at: 2053 4722 2053
Size: 2669 4463
KpnI G_GTAC'C
Cuts at: 2709 4457 2709
Size: 1748 5384
MfeI C'AATT_G
Cuts at: 2988 4731 2988
Size: 1743 5389
MluI A'CGCG_T
Cuts at: 7008 7008
Size: 7132
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3889 6246 6430 1305
Size: 2584 2357 184 2007
Fragments arranged by size:
2584 2357 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5310
Fragments arranged by size:
5310 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2307 4361 5090 5449 5608 518
Size: 648 1141 2054 729 359 159 2042
Fragments arranged by size:
2054 2042 1141 729 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2762 3398 4548 5225 6373 6618 1606
Size: 1156 636 1150 677 1148 245 2120
Fragments arranged by size:
2120 1156 1150 1148 677 636 245
NcoI C'CATG_G
Cuts at: 513 1065 2641 4096 513
Size: 552 1576 1455 3549
Fragments arranged by size:
3549 1576 1455 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7132
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7132
NotI GC'GGCC_GC
Cuts at: 4579 4579
Size: 7132
NsiI A_TGCA'T
Cuts at: 3241 3422 3241
Size: 181 6951
NspI r_CATG'y
Cuts at: 1132 1229 4490 6035 1132
Size: 97 3261 1545 2229
Fragments arranged by size:
3261 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7132
PciI A'CATG_T
Cuts at: 6031 6031
Size: 7132
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7132
PmeI GTTT'AAAC
Cuts at: 4436 4436
Size: 7132
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5201 5233 6714 6746 5201
Size: 32 1481 32 5587
Fragments arranged by size:
5587 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3094 4193 3094
Size: 1099 6033
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7132
PsiI TTA'TAA
Cuts at: 4702 4702
Size: 7132
PstI C_TGCA'G
Cuts at: 830 3294 3562 4484 830
Size: 2464 268 922 3478
Fragments arranged by size:
3478 2464 922 268
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7132
PvuII CAG'CTG
Cuts at: 2762 3398 2762
Size: 636 6496
SacI G_AGCT'C
Cuts at: 3576 3576
Size: 7132
SalI G'TCGA_C
Cuts at: 1936 4474 1936
Size: 2538 4594
SapI GCTCTTCn'nnn_
Cuts at: 3684 3684
Size: 7132
SbfI CC_TGCA'GG
Cuts at: 4484 4484
Size: 7132
ScaI AGT'ACT
Cuts at: 5380 7092 5380
Size: 1712 5420
SexAI A'CCwGG_T
Cuts at: 3739 3739
Size: 7132
SfcI C'TryA_G
Cuts at: 826 1045 3290 3558 4009 4480 5615 6296 6487
Size: 219 2245 268 451 471 1135 681 191
Cuts at: 6487 826
Size: 1471
Fragments arranged by size:
2245 1471 1135 681 471 451 268 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7132
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7132
SmaI CCC'GGG
Cuts at: 1872 4459 1872
Size: 2587 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5236 6137 6399 820
Size: 197 926 3293 901 262 1553
Fragments arranged by size:
3293 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4923 493
Size: 4430 2702
SpeI A'CTAG_T
Cuts at: 152 3638 152
Size: 3486 3646
SphI G_CATG'C
Cuts at: 1132 4490 1132
Size: 3358 3774
SspI AAT'ATT
Cuts at: 5 52 5056 5
Size: 47 5004 2081
Fragments arranged by size:
5004 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7132
StyI C'CwwG_G
Cuts at: 513 1065 2119 2532 2641 2791 4096 4278 4571
Size: 552 1054 413 109 150 1305 182 293
Cuts at: 4571 513
Size: 3074
Fragments arranged by size:
3074 1305 1054 552 413 293 182 150
109
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5159 5176 5329 5514 5959 325
Size: 1255 3579 17 153 185 445 1498
Fragments arranged by size:
3579 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5378 7090 96
Size: 274 80 33 51 1443 3401 1712 138
Fragments arranged by size:
3401 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2844 2871 2876 2903 3436 3468 4131 4163 7092
Size: 27 5 27 533 32 663 32 2929
Cuts at: 7092 7124 2844
Size: 32 2852
Fragments arranged by size:
2929 2852 663 533 32 32 32 27
27 5
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7132
XbaI T'CTAG_A
Cuts at: 4400 4468 4400
Size: 68 7064
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2603 2603
Size: 7132
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7132
XmaI C'CCGG_G
Cuts at: 1870 4457 1870
Size: 2587 4545
XmnI GAAnn'nnTTC
Cuts at: 2171 5261 2171
Size: 3090 4042
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4940 276
Size: 53 83 186 936 3406 2468
Fragments arranged by size:
3406 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SacI SalI SapI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BplI
BsiWI BstZ17I Bsu36I EcoNI FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI
PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI