Restriction map for
pFN21AE2778
(Circular) (Six-base) MAPSORT of: pFN21AE2778.seq Check: 2504 from: 1 to: 7048
pFN21AE2778 7048 bp
With 182 enzymes: SgfI *
July 4, 2020 18:17 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4858 278
Size: 53 83 186 936 3322 2468
Fragments arranged by size:
3322 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4391 1937
Size: 2454 4594
Acc65I G'GTAC_C
Cuts at: 2705 4369 2705
Size: 1664 5384
AclI AA'CG_TT
Cuts at: 1563 5175 5548 1563
Size: 3612 373 3063
Fragments arranged by size:
3612 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2158 4107 4271 5111 6495 6763 1632
Size: 526 1949 164 840 1384 268 1917
Fragments arranged by size:
1949 1917 1384 840 526 268 164
AfeI AGC'GCT
Cuts at: 2348 3527 2348
Size: 1179 5869
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6851
AflIII A'CryG_T
Cuts at: 1203 5947 6924 1203
Size: 4744 977 1327
Fragments arranged by size:
4744 1327 977
AgeI A'CCGG_T
Cuts at: 5879 5879
Size: 7048
AhdI GACnn_n'nnGTC
Cuts at: 5777 5777
Size: 7048
AleI CACnn'nnGTG
Cuts at: 1166 4277 1166
Size: 3111 3937
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7048
AlwNI CAG_nnn'CTG
Cuts at: 4196 4791 6363 4196
Size: 595 1572 4881
Fragments arranged by size:
4881 1572 595
ApaLI G'TGCA_C
Cuts at: 5105 6261 5105
Size: 1156 5892
ApoI r'AATT_y
Cuts at: 1493 3412 3654 4052 4357 4583 4735 6844 1493
Size: 1919 242 398 305 226 152 2109 1697
Fragments arranged by size:
2109 1919 1697 398 305 242 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2514 3284 3944 5602 160
Size: 872 1482 770 660 1658 1606
Fragments arranged by size:
1658 1606 1482 872 770 660
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7048
AvaI C'yCGr_G
Cuts at: 1870 1943 4373 1870
Size: 73 2430 4545
Fragments arranged by size:
4545 2430 73
BamHI G'GATC_C
Cuts at: 2393 4378 4745 2393
Size: 1985 367 4696
Fragments arranged by size:
4696 1985 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2156 2705 3223 4369 5824
Size: 325 217 627 369 549 518 1146 1455
Cuts at: 5824 618
Size: 1842
Fragments arranged by size:
1842 1455 1146 627 549 518 369 325
217
BanII G_rGCy'C
Cuts at: 1391 3576 4367 1391
Size: 2185 791 4072
Fragments arranged by size:
4072 2185 791
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3373 4275 4327 928
Size: 971 1474 902 52 3649
Fragments arranged by size:
3649 1474 971 902 52
BbvCI CC'TCA_GC
Cuts at: 3542 3542
Size: 7048
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5239 5273 1903
Size: 34 3302 34 3678
Fragments arranged by size:
3678 3302 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4940 6150 4940
Size: 1210 5838
BclI T'GATC_A
Cuts at: 1548 3088 3916 1548
Size: 1540 828 4680
Fragments arranged by size:
4680 1540 828
BfrBI ATG'CAT
Cuts at: 3239 3420 3239
Size: 181 6867
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5659 136
Size: 107 122 71 631 605 3987 1525
Fragments arranged by size:
3987 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2085 7043 2085
Size: 4958 2090
BlpI GC'TnA_GC
Cuts at: 4465 4465
Size: 7048
Bme1580I G_kGCm'C
Cuts at: 1790 2140 2159 5109 6265 1790
Size: 350 19 2950 1156 2573
Fragments arranged by size:
2950 2573 1156 350 19
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7048
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4779 5737 442
Size: 416 53 83 399 24 3362 958 1753
Fragments arranged by size:
3362 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7048
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2915 3609 4245 5708 1239
Size: 1676 694 636 1463 2579
Fragments arranged by size:
2579 1676 1463 694 636
Bpu10I CC'TnA_GC
Cuts at: 3542 3542
Size: 7048
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5173 6038 6336 5173
Size: 865 298 5885
Fragments arranged by size:
5885 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5711 882
Size: 617 4212 2219
Fragments arranged by size:
4212 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4839 493
Size: 711 3635 2702
Fragments arranged by size:
3635 2702 711
BsaBI GATnn'nnATC
Cuts at: 7042 7042
Size: 7048
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2358 4855 5237 5872
Size: 53 83 186 936 825 2497 382 635
Cuts at: 5872 275
Size: 1451
Fragments arranged by size:
2497 1451 936 825 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5481 5879 6153 6300 6711 5481
Size: 398 274 147 411 5818
Fragments arranged by size:
5818 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2871 2901 2946 2976 3436
Size: 30 165 30 1468 30 45 30 460
Cuts at: 3436 3466 4131 4161 1178
Size: 30 665 30 4065
Fragments arranged by size:
4065 1468 665 460 165 45 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2390 2970 3875 1186
Size: 1204 580 905 4359
Fragments arranged by size:
4359 1204 905 580
BseYI C'CCAG_C
Cuts at: 2281 6251 6747 2281
Size: 3970 496 2582
Fragments arranged by size:
3970 2582 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6475
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4498 5259 6287 1540
Size: 275 186 2497 761 1028 2301
Fragments arranged by size:
2497 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2632 3576 5109 5194 6265 2632
Size: 944 1533 85 1071 3415
Fragments arranged by size:
3415 1533 1071 944 85
BsmI GAATG_Cn'
Cuts at: 2497 4559 4652 2497
Size: 2062 93 4893
Fragments arranged by size:
4893 2062 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2793 2963 2793
Size: 170 6878
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2140 2159 2632 3576 4367 5109 5194
Size: 399 350 19 473 944 791 742 85
Cuts at: 5194 6265 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 944 791 742 473 399 350
85 19
BspEI T'CCGG_A
Cuts at: 6711 6711
Size: 7048
BspHI T'CATG_A
Cuts at: 3846 4937 6667 3846
Size: 1091 1730 4227
Fragments arranged by size:
4227 1730 1091
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4403 844
Size: 3559 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4935 1394
Size: 429 3112 3507
Fragments arranged by size:
3507 3112 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5543 5717 65
Size: 1183 4295 174 1396
Fragments arranged by size:
4295 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5692 5879 1700
Size: 110 3882 187 2869
Fragments arranged by size:
3882 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7048
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7048
BssSI C'ACGA_G
Cuts at: 2853 4170 5108 6120 2853
Size: 1317 938 1012 3781
Fragments arranged by size:
3781 1317 1012 938
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7048
BstBI TT'CG_AA
Cuts at: 5852 5852
Size: 7048
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7048
BstXI CCAn_nnnn'nTGG
Cuts at: 2309 2309
Size: 7048
BstYI r'GATC_y
Cuts at: 1972 2085 2393 3160 4313 4378 4745 5130 5147
Size: 113 308 767 1153 65 367 385 17
Cuts at: 5147 6599 6685 6697 7043 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1153 767 385 367 346 308
113 86 65 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2641 4096 513
Size: 552 1576 1455 3465
Fragments arranged by size:
3465 1576 1455 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6309
Fragments arranged by size:
6309 683 56
BtsI GCAGTG_nn'
Cuts at: 4564 5358 5378 4564
Size: 794 20 6234
Fragments arranged by size:
6234 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7048
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7013
DraI TTT'AAA
Cuts at: 3759 4352 3759
Size: 593 6455
DraIII CAC_nnn'GTG
Cuts at: 2858 2858
Size: 7048
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6055 809
Size: 507 4739 1802
Fragments arranged by size:
4739 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4495 5384 7013
Size: 54 1425 50 275 18 2665 889 1629
Cuts at: 7013 8
Size: 43
Fragments arranged by size:
2665 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4495 1537
Size: 275 2683 4090
Fragments arranged by size:
4090 2683 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2791 3684 4978 1353
Size: 1438 893 1294 3423
Fragments arranged by size:
3423 1438 1294 893
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5635 6009 6155 387
Size: 1064 138 4046 374 146 1280
Fragments arranged by size:
4046 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3574 3574
Size: 7048
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2158 2915 3609 4107 4245 4271 5111
Size: 393 526 757 694 498 138 26 840
Cuts at: 5111 5708 6495 6763 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 840 787 757 694 597 526 498
393 268 138 26
EcoO109I rG'GnC_Cy
Cuts at: 2788 3094 4193 2788
Size: 306 1099 5643
Fragments arranged by size:
5643 1099 306
EcoRI G'AATT_C
Cuts at: 4052 4357 4735 4052
Size: 305 378 6365
Fragments arranged by size:
6365 378 305
EcoRV GAT'ATC
Cuts at: 2449 2449
Size: 7048
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2188 2413 2759 3338 2188
Size: 225 346 579 5898
Fragments arranged by size:
5898 579 346 225
FspI TGC'GCA
Cuts at: 5554 5554
Size: 7048
HaeII r_GCGC'y
Cuts at: 1678 2350 3274 3529 6195 1678
Size: 672 924 255 2666 2531
Fragments arranged by size:
2666 2531 924 672 255
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2077 2109 2732 2764 2946
Size: 32 135 32 505 32 623 32 182
Cuts at: 2946 2978 5689 5721 5763 5795 1373
Size: 32 2711 32 42 32 2626
Fragments arranged by size:
2711 2626 623 505 182 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2053 2529 4392 4638 669
Size: 988 281 115 476 1863 246 3079
Fragments arranged by size:
3079 1863 988 476 281 246 115
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7048
HpaI GTT'AAC
Cuts at: 2053 4638 2053
Size: 2585 4463
KpnI G_GTAC'C
Cuts at: 2709 4373 2709
Size: 1664 5384
MfeI C'AATT_G
Cuts at: 2988 4647 2988
Size: 1659 5389
MluI A'CGCG_T
Cuts at: 6924 6924
Size: 7048
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3889 6162 6346 1305
Size: 2584 2273 184 2007
Fragments arranged by size:
2584 2273 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5226
Fragments arranged by size:
5226 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2307 4277 5006 5365 5524 518
Size: 648 1141 1970 729 359 159 2042
Fragments arranged by size:
2042 1970 1141 729 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2762 3398 4464 5141 6289 6534 1606
Size: 1156 636 1066 677 1148 245 2120
Fragments arranged by size:
2120 1156 1148 1066 677 636 245
NcoI C'CATG_G
Cuts at: 513 1065 2641 4096 513
Size: 552 1576 1455 3465
Fragments arranged by size:
3465 1576 1455 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7048
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7048
NotI GC'GGCC_GC
Cuts at: 4495 4495
Size: 7048
NsiI A_TGCA'T
Cuts at: 3241 3422 3241
Size: 181 6867
NspI r_CATG'y
Cuts at: 1132 1229 4406 5951 1132
Size: 97 3177 1545 2229
Fragments arranged by size:
3177 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7048
PciI A'CATG_T
Cuts at: 5947 5947
Size: 7048
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7048
PmeI GTTT'AAAC
Cuts at: 4352 4352
Size: 7048
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5117 5149 6630 6662 5117
Size: 32 1481 32 5503
Fragments arranged by size:
5503 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3094 4193 3094
Size: 1099 5949
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7048
PsiI TTA'TAA
Cuts at: 4618 4618
Size: 7048
PstI C_TGCA'G
Cuts at: 830 3294 3562 4400 830
Size: 2464 268 838 3478
Fragments arranged by size:
3478 2464 838 268
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7048
PvuII CAG'CTG
Cuts at: 2762 3398 2762
Size: 636 6412
SacI G_AGCT'C
Cuts at: 3576 3576
Size: 7048
SalI G'TCGA_C
Cuts at: 1936 4390 1936
Size: 2454 4594
SapI GCTCTTCn'nnn_
Cuts at: 3684 3684
Size: 7048
SbfI CC_TGCA'GG
Cuts at: 4400 4400
Size: 7048
ScaI AGT'ACT
Cuts at: 5296 7008 5296
Size: 1712 5336
SexAI A'CCwGG_T
Cuts at: 3739 3739
Size: 7048
SfcI C'TryA_G
Cuts at: 826 1045 3290 3558 4009 4396 5531 6212 6403
Size: 219 2245 268 451 387 1135 681 191
Cuts at: 6403 826
Size: 1471
Fragments arranged by size:
2245 1471 1135 681 451 387 268 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7048
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7048
SmaI CCC'GGG
Cuts at: 1872 4375 1872
Size: 2503 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5152 6053 6315 820
Size: 197 926 3209 901 262 1553
Fragments arranged by size:
3209 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4839 493
Size: 4346 2702
SpeI A'CTAG_T
Cuts at: 152 3638 152
Size: 3486 3562
SphI G_CATG'C
Cuts at: 1132 4406 1132
Size: 3274 3774
SspI AAT'ATT
Cuts at: 5 52 4972 5
Size: 47 4920 2081
Fragments arranged by size:
4920 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7048
StyI C'CwwG_G
Cuts at: 513 1065 2119 2532 2641 2791 4096 4487 513
Size: 552 1054 413 109 150 1305 391 3074
Fragments arranged by size:
3074 1305 1054 552 413 391 150 109
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5075 5092 5245 5430 5875 325
Size: 1255 3495 17 153 185 445 1498
Fragments arranged by size:
3495 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5294 7006 96
Size: 274 80 33 51 1443 3317 1712 138
Fragments arranged by size:
3317 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2844 2871 2876 2903 3436 3468 4131 4163 7008
Size: 27 5 27 533 32 663 32 2845
Cuts at: 7008 7040 2844
Size: 32 2852
Fragments arranged by size:
2852 2845 663 533 32 32 32 27
27 5
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7048
XbaI T'CTAG_A
Cuts at: 4316 4384 4316
Size: 68 6980
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2603 2603
Size: 7048
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7048
XmaI C'CCGG_G
Cuts at: 1870 4373 1870
Size: 2503 4545
XmnI GAAnn'nnTTC
Cuts at: 2171 5177 2171
Size: 3006 4042
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4856 276
Size: 53 83 186 936 3322 2468
Fragments arranged by size:
3322 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SacI SalI SapI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BplI
BsiWI BstZ17I Bsu36I EcoNI FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI
PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI