Restriction map for
pFN21AE2776
(Circular) (Six-base) MAPSORT of: pFN21AE2776.seq Check: 9648 from: 1 to: 5920
pFN21AE2776 5920 bp
With 182 enzymes: SgfI *
February 20, 2021 22:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2801 2882 2801
Size: 81 5839
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3730 278
Size: 53 83 186 936 2194 2468
Fragments arranged by size:
2468 2194 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3263 1937
Size: 1326 4594
Acc65I G'GTAC_C
Cuts at: 3241 3241
Size: 5920
AclI AA'CG_TT
Cuts at: 1563 4047 4420 1563
Size: 2484 373 3063
Fragments arranged by size:
3063 2484 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2420 3983 5367 5635 1632
Size: 788 1563 1384 268 1917
Fragments arranged by size:
1917 1563 1384 788 268
AfeI AGC'GCT
Cuts at: 2034 2034
Size: 5920
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5723
AflIII A'CryG_T
Cuts at: 1203 2529 4819 5796 1203
Size: 1326 2290 977 1327
Fragments arranged by size:
2290 1327 1326 977
AgeI A'CCGG_T
Cuts at: 4751 4751
Size: 5920
AhdI GACnn_n'nnGTC
Cuts at: 4649 4649
Size: 5920
AleI CACnn'nnGTG
Cuts at: 1166 2876 1166
Size: 1710 4210
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5920
AlwNI CAG_nnn'CTG
Cuts at: 2632 2694 3178 3663 5235 2632
Size: 62 484 485 1572 3317
Fragments arranged by size:
3317 1572 485 484 62
ApaLI G'TGCA_C
Cuts at: 3977 5133 3977
Size: 1156 4764
ApoI r'AATT_y
Cuts at: 1493 2194 2298 2347 2419 2762 3035 3229 3455
Size: 701 104 49 72 343 273 194 226
Cuts at: 3455 3607 5716 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 701 343 273 226 194 152
104 72 49
AseI AT'TA_AT
Cuts at: 160 1032 4474 160
Size: 872 3442 1606
Fragments arranged by size:
3442 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5920
AvaI C'yCGr_G
Cuts at: 1870 1943 2733 2846 3245 1870
Size: 73 790 113 399 4545
Fragments arranged by size:
4545 790 399 113 73
BamHI G'GATC_C
Cuts at: 3166 3250 3617 3166
Size: 84 367 5469
Fragments arranged by size:
5469 367 84
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2441 2867 3241 4696 618
Size: 325 217 627 654 426 374 1455 1842
Fragments arranged by size:
1842 1455 654 627 426 374 325 217
BanII G_rGCy'C
Cuts at: 1391 2077 3239 1391
Size: 686 1162 4072
Fragments arranged by size:
4072 1162 686
BbeI G_GCGC'C
Cuts at: 2445 2445
Size: 5920
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2970 928
Size: 971 1071 3878
Fragments arranged by size:
3878 1071 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4111 4145 1903
Size: 34 50 34 2090 34 3678
Fragments arranged by size:
3678 2090 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3812 5022 3812
Size: 1210 4710
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5920
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2939 4531 136
Size: 107 122 71 631 605 1267 1592 1525
Fragments arranged by size:
1592 1525 1267 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5915 5915
Size: 5920
BlpI GC'TnA_GC
Cuts at: 3337 3337
Size: 5920
Bme1580I G_kGCm'C
Cuts at: 1790 2132 2653 3981 5137 1790
Size: 342 521 1328 1156 2573
Fragments arranged by size:
2573 1328 1156 521 342
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5920
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2682 3651 4609
Size: 416 53 83 399 24 1265 969 958
Cuts at: 4609 442
Size: 1753
Fragments arranged by size:
1753 1265 969 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5920
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2091 2091
Size: 5920
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2099 2141 4580 1239
Size: 860 42 2439 2579
Fragments arranged by size:
2579 2439 860 42
Bpu10I CC'TnA_GC
Cuts at: 2359 2359
Size: 5920
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4045 4910 5208 4045
Size: 865 298 4757
Fragments arranged by size:
4757 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4583 882
Size: 617 3084 2219
Fragments arranged by size:
3084 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2781 2826 3711 493
Size: 711 1577 45 885 2702
Fragments arranged by size:
2702 1577 885 711 45
BsaBI GATnn'nnATC
Cuts at: 5914 5914
Size: 5920
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2442 2970 3727 4109
Size: 53 83 186 936 909 528 757 382
Cuts at: 4109 4744 275
Size: 635 1451
Fragments arranged by size:
1451 936 909 757 635 528 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2393 4353 4751 5025 5172 5583 2393
Size: 1960 398 274 147 411 2730
Fragments arranged by size:
2730 1960 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3035 3065 3077 3107 3139
Size: 30 165 30 1632 30 12 30 32
Cuts at: 3139 3169 1178
Size: 30 3929
Fragments arranged by size:
3929 1632 165 32 30 30 30 30
30 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2630 3147 3150 1186
Size: 1444 517 3 3956
Fragments arranged by size:
3956 1444 517 3
BseYI C'CCAG_C
Cuts at: 2601 2652 5123 5619 2601
Size: 51 2471 496 2902
Fragments arranged by size:
2902 2471 496 51
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2234 2628 2707 3073 1163
Size: 573 498 394 79 366 4010
Fragments arranged by size:
4010 573 498 394 366 79
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2025 3370 4131 5159 1540
Size: 275 186 24 1345 761 1028 2301
Fragments arranged by size:
2301 1345 1028 761 275 186 24
BsiHKAI G_wGCw'C
Cuts at: 2663 3981 4066 5137 2663
Size: 1318 85 1071 3446
Fragments arranged by size:
3446 1318 1071 85
BsmI GAATG_Cn'
Cuts at: 3431 3524 3431
Size: 93 5827
BsmBI CGTCTCn'nnnn_
Cuts at: 2763 2763
Size: 5920
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2077 2132 2653 2663 3239 3981 4066
Size: 399 287 55 521 10 576 742 85
Cuts at: 4066 5137 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 576 521 399 287 85
55 10
BspEI T'CCGG_A
Cuts at: 5583 5583
Size: 5920
BspHI T'CATG_A
Cuts at: 3809 5539 3809
Size: 1730 4190
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2322 2801 2882 3275 844
Size: 1478 479 81 393 3489
Fragments arranged by size:
3489 1478 479 393 81
BsrBI CCG'CTC
Cuts at: 1394 1823 3807 1394
Size: 429 1984 3507
Fragments arranged by size:
3507 1984 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2274 4415 4589 65
Size: 1183 1026 2141 174 1396
Fragments arranged by size:
2141 1396 1183 1026 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3121 4564 4751 1700
Size: 110 1311 1443 187 2869
Fragments arranged by size:
2869 1443 1311 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5920
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5920
BssSI C'ACGA_G
Cuts at: 2104 2920 3980 4992 2104
Size: 816 1060 1012 3032
Fragments arranged by size:
3032 1060 1012 816
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5920
BstBI TT'CG_AA
Cuts at: 4724 4724
Size: 5920
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5920
BstYI r'GATC_y
Cuts at: 1972 3166 3250 3617 4002 4019 5471 5557 5569
Size: 1194 84 367 385 17 1452 86 12
Cuts at: 5569 5915 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1194 385 367 346 86 84
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2674 513
Size: 552 1609 3759
Fragments arranged by size:
3759 1609 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5181
Fragments arranged by size:
5181 683 56
BtsI GCAGTG_nn'
Cuts at: 2518 2663 3436 4230 4250 2518
Size: 145 773 794 20 4188
Fragments arranged by size:
4188 794 773 145 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5920
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5885
DraI TTT'AAA
Cuts at: 3040 3224 3040
Size: 184 5736
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2750 4927 809
Size: 507 1434 2177 1802
Fragments arranged by size:
2177 1802 1434 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2022 3367 4256
Size: 54 1425 50 275 18 192 1345 889
Cuts at: 4256 5885 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1345 889 275 192 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2022 3367 1537
Size: 275 210 1345 4090
Fragments arranged by size:
4090 1345 275 210
EarI CTCTTCn'nnn_
Cuts at: 1353 3850 1353
Size: 2497 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4507 4881 5027 387
Size: 1064 138 2918 374 146 1280
Fragments arranged by size:
2918 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2099 2141 2420 3983 4580 5367 5635
Size: 393 467 42 279 1563 597 787 268
Cuts at: 5635 1239
Size: 1524
Fragments arranged by size:
1563 1524 787 597 467 393 279 268
42
EcoO109I rG'GnC_Cy
Cuts at: 2729 2996 2729
Size: 267 5653
EcoRI G'AATT_C
Cuts at: 2298 3229 3607 2298
Size: 931 378 4611
Fragments arranged by size:
4611 931 378
FspI TGC'GCA
Cuts at: 4426 4426
Size: 5920
HaeII r_GCGC'y
Cuts at: 1678 2036 2045 2445 2671 5067 1678
Size: 358 9 400 226 2396 2531
Fragments arranged by size:
2531 2396 400 358 226 9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2903 2935 3035 3067 4561
Size: 32 135 32 1331 32 100 32 1494
Cuts at: 4561 4593 4635 4667 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1494 1331 135 100 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3264 3510 669
Size: 988 281 1326 246 3079
Fragments arranged by size:
3079 1326 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5920
HpaI GTT'AAC
Cuts at: 3510 3510
Size: 5920
KasI G'GCGC_C
Cuts at: 2441 2441
Size: 5920
KpnI G_GTAC'C
Cuts at: 3245 3245
Size: 5920
MfeI C'AATT_G
Cuts at: 3519 3519
Size: 5920
MluI A'CGCG_T
Cuts at: 5796 5796
Size: 5920
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5034 5218 1305
Size: 3729 184 2007
Fragments arranged by size:
3729 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4098
Fragments arranged by size:
4098 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2534 2876 3878 4237 4396 518
Size: 648 1368 342 1002 359 159 2042
Fragments arranged by size:
2042 1368 1002 648 359 342 159
MspA1I CmG'CkG
Cuts at: 1606 2562 3336 4013 5161 5406 1606
Size: 956 774 677 1148 245 2120
Fragments arranged by size:
2120 1148 956 774 677 245
NarI GG'CG_CC
Cuts at: 2442 2442
Size: 5920
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5368
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5920
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5920
NotI GC'GGCC_GC
Cuts at: 3367 3367
Size: 5920
NspI r_CATG'y
Cuts at: 1132 1229 2533 3278 4823 1132
Size: 97 1304 745 1545 2229
Fragments arranged by size:
2229 1545 1304 745 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5920
PciI A'CATG_T
Cuts at: 2529 4819 2529
Size: 2290 3630
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2257 1108
Size: 1149 4771
PfoI T'CCnGG_A
Cuts at: 2015 2250 2015
Size: 235 5685
PmeI GTTT'AAAC
Cuts at: 3224 3224
Size: 5920
PmlI CAC'GTG
Cuts at: 2781 2781
Size: 5920
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3989 4021 5502 5534 3989
Size: 32 1481 32 4375
Fragments arranged by size:
4375 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5920
PsiI TTA'TAA
Cuts at: 3490 3490
Size: 5920
PstI C_TGCA'G
Cuts at: 830 2319 2719 3272 830
Size: 1489 400 553 3478
Fragments arranged by size:
3478 1489 553 400
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5920
PvuII CAG'CTG
Cuts at: 2562 2562
Size: 5920
SalI G'TCGA_C
Cuts at: 1936 3262 1936
Size: 1326 4594
SbfI CC_TGCA'GG
Cuts at: 3272 3272
Size: 5920
ScaI AGT'ACT
Cuts at: 4168 5880 4168
Size: 1712 4208
SfcI C'TryA_G
Cuts at: 826 1045 2315 2715 3268 4403 5084 5275 826
Size: 219 1270 400 553 1135 681 191 1471
Fragments arranged by size:
1471 1270 1135 681 553 400 219 191
SfoI GGC'GCC
Cuts at: 2443 2443
Size: 5920
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5920
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5920
SmaI CCC'GGG
Cuts at: 1872 2735 3247 1872
Size: 863 512 4545
Fragments arranged by size:
4545 863 512
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4024 4925 5187 820
Size: 197 926 2081 901 262 1553
Fragments arranged by size:
2081 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3711 493
Size: 3218 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5920
SphI G_CATG'C
Cuts at: 1132 3278 1132
Size: 2146 3774
SspI AAT'ATT
Cuts at: 5 52 3070 3844 5
Size: 47 3018 774 2081
Fragments arranged by size:
3018 2081 774 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5920
StyI C'CwwG_G
Cuts at: 513 1065 2098 2188 3359 513
Size: 552 1033 90 1171 3074
Fragments arranged by size:
3074 1171 1033 552 90
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3947 3964 4117 4302 4747 325
Size: 1255 2367 17 153 185 445 1498
Fragments arranged by size:
2367 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2337 4166 5878
Size: 274 80 33 51 1443 360 1829 1712
Cuts at: 5878 96
Size: 138
Fragments arranged by size:
1829 1712 1443 360 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3139 3171 5880 5912 3139
Size: 32 2709 32 3147
Fragments arranged by size:
3147 2709 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2504 2969 1316
Size: 1188 465 4267
Fragments arranged by size:
4267 1188 465
XbaI T'CTAG_A
Cuts at: 3256 3256
Size: 5920
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2254 2258 2830 2254
Size: 4 572 5344
Fragments arranged by size:
5344 572 4
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5920
XmaI C'CCGG_G
Cuts at: 1870 2733 3245 1870
Size: 863 512 4545
Fragments arranged by size:
4545 863 512
XmnI GAAnn'nnTTC
Cuts at: 4049 4049
Size: 5920
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3728 276
Size: 53 83 186 936 2194 2468
Fragments arranged by size:
2468 2194 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PshAI PsiI PstI PvuI PvuII SalI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbvCI BfrBI BsiWI
BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI
FseI FspAI NaeI NgoMIV NruI NsiI PacI PpuMI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI
SexAI SfiI SrfI SwaI