Restriction map for
pFN21AE2775
(Circular) (Six-base) MAPSORT of: pFN21AE2775.seq Check: 9046 from: 1 to: 6589
pFN21AE2775 6589 bp
With 182 enzymes: SgfI *
February 20, 2021 22:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2801 2882 3722 2801
Size: 81 840 5668
Fragments arranged by size:
5668 840 81
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4399 278
Size: 53 83 186 936 2863 2468
Fragments arranged by size:
2863 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3932 1937
Size: 1995 4594
Acc65I G'GTAC_C
Cuts at: 3910 3910
Size: 6589
AclI AA'CG_TT
Cuts at: 1563 4716 5089 1563
Size: 3153 373 3063
Fragments arranged by size:
3153 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2420 4652 6036 6304 1632
Size: 788 2232 1384 268 1917
Fragments arranged by size:
2232 1917 1384 788 268
AfeI AGC'GCT
Cuts at: 2034 2034
Size: 6589
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6392
AflIII A'CryG_T
Cuts at: 1203 2529 5488 6465 1203
Size: 1326 2959 977 1327
Fragments arranged by size:
2959 1327 1326 977
AgeI A'CCGG_T
Cuts at: 5420 5420
Size: 6589
AhdI GACnn_n'nnGTC
Cuts at: 3456 5318 3456
Size: 1862 4727
AleI CACnn'nnGTG
Cuts at: 1166 2876 1166
Size: 1710 4879
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6589
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3326 3358 3326
Size: 32 6557
AlwNI CAG_nnn'CTG
Cuts at: 2632 2694 3178 4332 5904 2632
Size: 62 484 1154 1572 3317
Fragments arranged by size:
3317 1572 1154 484 62
ApaI G_GGCC'C
Cuts at: 3557 3557
Size: 6589
ApaLI G'TGCA_C
Cuts at: 4646 5802 4646
Size: 1156 5433
ApoI r'AATT_y
Cuts at: 1493 2194 2298 2347 2419 2762 3035 3406 3898
Size: 701 104 49 72 343 273 371 492
Cuts at: 3898 4124 4276 6385 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 701 492 371 343 273 226
152 104 72 49
AseI AT'TA_AT
Cuts at: 160 1032 5143 160
Size: 872 4111 1606
Fragments arranged by size:
4111 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6589
AvaI C'yCGr_G
Cuts at: 1870 1943 2733 2846 3539 3914 1870
Size: 73 790 113 693 375 4545
Fragments arranged by size:
4545 790 693 375 113 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3724 3757 3724
Size: 33 6556
BamHI G'GATC_C
Cuts at: 3166 3919 4286 3166
Size: 753 367 5469
Fragments arranged by size:
5469 753 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2441 2867 3910 5365 618
Size: 325 217 627 654 426 1043 1455 1842
Fragments arranged by size:
1842 1455 1043 654 627 426 325 217
BanII G_rGCy'C
Cuts at: 1391 2077 3557 3564 3908 1391
Size: 686 1480 7 344 4072
Fragments arranged by size:
4072 1480 686 344 7
BbeI G_GCGC'C
Cuts at: 2445 2445
Size: 6589
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2970 928
Size: 971 1071 4547
Fragments arranged by size:
4547 1071 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4780 4814 1903
Size: 34 50 34 2759 34 3678
Fragments arranged by size:
3678 2759 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3752 4481 5691 3752
Size: 729 1210 4650
Fragments arranged by size:
4650 1210 729
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6589
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2939 5200 136
Size: 107 122 71 631 605 1267 2261 1525
Fragments arranged by size:
2261 1525 1267 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6584 6584
Size: 6589
BlpI GC'TnA_GC
Cuts at: 4006 4006
Size: 6589
Bme1580I G_kGCm'C
Cuts at: 1790 2132 2653 3557 3754 4650 5806 1790
Size: 342 521 904 197 896 1156 2573
Fragments arranged by size:
2573 1156 904 896 521 342 197
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6589
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2682 4320 5278
Size: 416 53 83 399 24 1265 1638 958
Cuts at: 5278 442
Size: 1753
Fragments arranged by size:
1753 1638 1265 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6589
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2091 2091
Size: 6589
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2099 2141 3650 5249 1239
Size: 860 42 1509 1599 2579
Fragments arranged by size:
2579 1599 1509 860 42
Bpu10I CC'TnA_GC
Cuts at: 2359 2359
Size: 6589
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3347 4714 5579 5877 3347
Size: 1367 865 298 4059
Fragments arranged by size:
4059 1367 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5252 882
Size: 617 3753 2219
Fragments arranged by size:
3753 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2781 2826 4380 493
Size: 711 1577 45 1554 2702
Fragments arranged by size:
2702 1577 1554 711 45
BsaBI GATnn'nnATC
Cuts at: 6583 6583
Size: 6589
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2442 2970 3840 4396
Size: 53 83 186 936 909 528 870 556
Cuts at: 4396 4778 5413 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 909 870 635 556 528 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 2393 3455 5022 5420 5694 5841 6252 2393
Size: 1062 1567 398 274 147 411 2730
Fragments arranged by size:
2730 1567 1062 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3035 3065 3077 3107 3139
Size: 30 165 30 1632 30 12 30 32
Cuts at: 3139 3169 1178
Size: 30 4598
Fragments arranged by size:
4598 1632 165 32 30 30 30 30
30 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2630 3147 3150 1186
Size: 1444 517 3 4625
Fragments arranged by size:
4625 1444 517 3
BseYI C'CCAG_C
Cuts at: 2601 2652 3549 5792 6288 2601
Size: 51 897 2243 496 2902
Fragments arranged by size:
2902 2243 897 496 51
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2234 2628 2707 3073 3700 3859 1163
Size: 573 498 394 79 366 627 159 3893
Fragments arranged by size:
3893 627 573 498 394 366 159 79
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2025 4039 4800 5828 1540
Size: 275 186 24 2014 761 1028 2301
Fragments arranged by size:
2301 2014 1028 761 275 186 24
BsiHKAI G_wGCw'C
Cuts at: 2663 3536 4650 4735 5806 2663
Size: 873 1114 85 1071 3446
Fragments arranged by size:
3446 1114 1071 873 85
BsmI GAATG_Cn'
Cuts at: 3611 4100 4193 3611
Size: 489 93 6007
Fragments arranged by size:
6007 489 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2763 3580 2763
Size: 817 5772
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2077 2132 2653 2663 3536 3557 3564
Size: 399 287 55 521 10 873 21 7
Cuts at: 3564 3754 3908 4650 4735 5806 1391
Size: 190 154 742 85 1071 2174
Fragments arranged by size:
2174 1071 873 742 521 399 287 190
154 85 55 21 10 7
BspEI T'CCGG_A
Cuts at: 6252 6252
Size: 6589
BspHI T'CATG_A
Cuts at: 4478 6208 4478
Size: 1730 4859
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2322 2801 2882 3722 3794 3944 844
Size: 1478 479 81 840 72 150 3489
Fragments arranged by size:
3489 1478 840 479 150 81 72
BsrBI CCG'CTC
Cuts at: 1394 1823 4476 1394
Size: 429 2653 3507
Fragments arranged by size:
3507 2653 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2274 5084 5258 65
Size: 1183 1026 2810 174 1396
Fragments arranged by size:
2810 1396 1183 1026 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3121 3842 5233 5420 1700
Size: 110 1311 721 1391 187 2869
Fragments arranged by size:
2869 1391 1311 721 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6589
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6589
BssSI C'ACGA_G
Cuts at: 2104 2920 4649 5661 2104
Size: 816 1729 1012 3032
Fragments arranged by size:
3032 1729 1012 816
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3606 3675 794
Size: 2812 69 3708
Fragments arranged by size:
3708 2812 69
BstBI TT'CG_AA
Cuts at: 5393 5393
Size: 6589
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6589
BstYI r'GATC_y
Cuts at: 1972 3166 3919 4286 4671 4688 6140 6226 6238
Size: 1194 753 367 385 17 1452 86 12
Cuts at: 6238 6584 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1194 753 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3696 3696
Size: 6589
BtgI C'CryG_G
Cuts at: 513 1065 2674 513
Size: 552 1609 4428
Fragments arranged by size:
4428 1609 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5850
Fragments arranged by size:
5850 683 56
BtsI GCAGTG_nn'
Cuts at: 2518 2663 3772 4105 4899 4919 2518
Size: 145 1109 333 794 20 4188
Fragments arranged by size:
4188 1109 794 333 145 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6589
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6554
DraI TTT'AAA
Cuts at: 3040 3893 3040
Size: 853 5736
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2750 5596 809
Size: 507 1434 2846 1802
Fragments arranged by size:
2846 1802 1434 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2022 3844 4036
Size: 54 1425 50 275 18 192 1822 192
Cuts at: 4036 4925 6554 8
Size: 889 1629 43
Fragments arranged by size:
1822 1629 1425 889 275 192 192 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2022 4036 1537
Size: 275 210 2014 4090
Fragments arranged by size:
4090 2014 275 210
EarI CTCTTCn'nnn_
Cuts at: 1353 4519 1353
Size: 3166 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5176 5550 5696 387
Size: 1064 138 3587 374 146 1280
Fragments arranged by size:
3587 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2099 2141 2420 3650 4652 5249 6036
Size: 393 467 42 279 1230 1002 597 787
Cuts at: 6036 6304 1239
Size: 268 1524
Fragments arranged by size:
1524 1230 1002 787 597 467 393 279
268 42
EcoNI CCTnn'n_nnAGG
Cuts at: 3629 3695 3629
Size: 66 6523
EcoO109I rG'GnC_Cy
Cuts at: 2729 2996 3450 3553 2729
Size: 267 454 103 5765
Fragments arranged by size:
5765 454 267 103
EcoRI G'AATT_C
Cuts at: 2298 3898 4276 2298
Size: 1600 378 4611
Fragments arranged by size:
4611 1600 378
FspI TGC'GCA
Cuts at: 5095 5095
Size: 6589
HaeII r_GCGC'y
Cuts at: 1678 2036 2045 2445 2671 5736 1678
Size: 358 9 400 226 3065 2531
Fragments arranged by size:
3065 2531 400 358 226 9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2903 2935 3035 3067 3442
Size: 32 135 32 1331 32 100 32 375
Cuts at: 3442 3474 3560 3592 5230 5262 5304 5336 1373
Size: 32 86 32 1638 32 42 32 2626
Fragments arranged by size:
2626 1638 1331 375 135 100 86 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3933 4179 669
Size: 988 281 1995 246 3079
Fragments arranged by size:
3079 1995 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6589
HpaI GTT'AAC
Cuts at: 4179 4179
Size: 6589
KasI G'GCGC_C
Cuts at: 2441 2441
Size: 6589
KpnI G_GTAC'C
Cuts at: 3914 3914
Size: 6589
MfeI C'AATT_G
Cuts at: 4188 4188
Size: 6589
MluI A'CGCG_T
Cuts at: 6465 6465
Size: 6589
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5703 5887 1305
Size: 4398 184 2007
Fragments arranged by size:
4398 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4767
Fragments arranged by size:
4767 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2534 2876 4547 4906 5065 518
Size: 648 1368 342 1671 359 159 2042
Fragments arranged by size:
2042 1671 1368 648 359 342 159
MspA1I CmG'CkG
Cuts at: 1606 2562 3498 4005 4682 5830 6075 1606
Size: 956 936 507 677 1148 245 2120
Fragments arranged by size:
2120 1148 956 936 677 507 245
NaeI GCC'GGC
Cuts at: 3844 3844
Size: 6589
NarI GG'CG_CC
Cuts at: 2442 2442
Size: 6589
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6037
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6589
NgoMIV G'CCGG_C
Cuts at: 3842 3842
Size: 6589
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6589
NotI GC'GGCC_GC
Cuts at: 4036 4036
Size: 6589
NspI r_CATG'y
Cuts at: 1132 1229 2533 3947 5492 1132
Size: 97 1304 1414 1545 2229
Fragments arranged by size:
2229 1545 1414 1304 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6589
PciI A'CATG_T
Cuts at: 2529 5488 2529
Size: 2959 3630
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2257 1108
Size: 1149 5440
PfoI T'CCnGG_A
Cuts at: 2015 2250 2015
Size: 235 6354
PmeI GTTT'AAAC
Cuts at: 3893 3893
Size: 6589
PmlI CAC'GTG
Cuts at: 2781 2781
Size: 6589
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4658 4690 6171 6203 4658
Size: 32 1481 32 5044
Fragments arranged by size:
5044 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3450 3450
Size: 6589
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6589
PsiI TTA'TAA
Cuts at: 3476 4159 3476
Size: 683 5906
PspOMI G'GGCC_C
Cuts at: 3553 3553
Size: 6589
PstI C_TGCA'G
Cuts at: 830 2319 2719 3941 830
Size: 1489 400 1222 3478
Fragments arranged by size:
3478 1489 1222 400
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6589
PvuII CAG'CTG
Cuts at: 2562 3498 2562
Size: 936 5653
SalI G'TCGA_C
Cuts at: 1936 3931 1936
Size: 1995 4594
SbfI CC_TGCA'GG
Cuts at: 3941 3941
Size: 6589
ScaI AGT'ACT
Cuts at: 4837 6549 4837
Size: 1712 4877
SfcI C'TryA_G
Cuts at: 826 1045 2315 2715 3937 5072 5753 5944 826
Size: 219 1270 400 1222 1135 681 191 1471
Fragments arranged by size:
1471 1270 1222 1135 681 400 219 191
SfoI GGC'GCC
Cuts at: 2443 2443
Size: 6589
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6589
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6589
SmaI CCC'GGG
Cuts at: 1872 2735 3916 1872
Size: 863 1181 4545
Fragments arranged by size:
4545 1181 863
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3362 4693 5594 5856 820
Size: 197 926 1419 1331 901 262 1553
Fragments arranged by size:
1553 1419 1331 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4380 493
Size: 3887 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6589
SphI G_CATG'C
Cuts at: 1132 3947 1132
Size: 2815 3774
SspI AAT'ATT
Cuts at: 5 52 3070 4513 5
Size: 47 3018 1443 2081
Fragments arranged by size:
3018 2081 1443 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6589
StyI C'CwwG_G
Cuts at: 513 1065 2098 2188 3345 3564 3641 4028 513
Size: 552 1033 90 1157 219 77 387 3074
Fragments arranged by size:
3074 1157 1033 552 387 219 90 77
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3696 4616 4633 4786 4971 5416 325
Size: 1255 2116 920 17 153 185 445 1498
Fragments arranged by size:
2116 1498 1255 920 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2337 4835 6547
Size: 274 80 33 51 1443 360 2498 1712
Cuts at: 6547 96
Size: 138
Fragments arranged by size:
2498 1712 1443 360 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3139 3171 6549 6581 3139
Size: 32 3378 32 3147
Fragments arranged by size:
3378 3147 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2504 2969 3572 1316
Size: 1188 465 603 4333
Fragments arranged by size:
4333 1188 603 465
XbaI T'CTAG_A
Cuts at: 3925 3925
Size: 6589
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2254 2258 2830 2254
Size: 4 572 6013
Fragments arranged by size:
6013 572 4
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6589
XmaI C'CCGG_G
Cuts at: 1870 2733 3914 1870
Size: 863 1181 4545
Fragments arranged by size:
4545 1181 863
XmnI GAAnn'nnTTC
Cuts at: 4718 4718
Size: 6589
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4397 276
Size: 53 83 186 936 2863 2468
Fragments arranged by size:
2863 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AscI AvrII BbvCI BfrBI BsiWI BstXI BstZ17I DraIII
EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI
PspXI PsrI RsrII SacI SacII SanDI SapI SexAI
SfiI SrfI SwaI