Restriction map for
pFN21AE2774
(Circular) (Six-base) MAPSORT of: pFN21AE2774.seq Check: 9218 from: 1 to: 6820
pFN21AE2774 6820 bp
With 182 enzymes: SgfI *
February 20, 2021 22:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2801 2882 3722 2801
Size: 81 840 5899
Fragments arranged by size:
5899 840 81
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4630 278
Size: 53 83 186 936 3094 2468
Fragments arranged by size:
3094 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4163 1937
Size: 2226 4594
Acc65I G'GTAC_C
Cuts at: 4141 4141
Size: 6820
AclI AA'CG_TT
Cuts at: 1563 4947 5320 1563
Size: 3384 373 3063
Fragments arranged by size:
3384 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2420 4883 6267 6535 1632
Size: 788 2463 1384 268 1917
Fragments arranged by size:
2463 1917 1384 788 268
AfeI AGC'GCT
Cuts at: 2034 2034
Size: 6820
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6623
AflIII A'CryG_T
Cuts at: 1203 2529 5719 6696 1203
Size: 1326 3190 977 1327
Fragments arranged by size:
3190 1327 1326 977
AgeI A'CCGG_T
Cuts at: 5651 5651
Size: 6820
AhdI GACnn_n'nnGTC
Cuts at: 3456 5549 3456
Size: 2093 4727
AleI CACnn'nnGTG
Cuts at: 1166 2876 1166
Size: 1710 5110
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6820
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3326 3358 3326
Size: 32 6788
AlwNI CAG_nnn'CTG
Cuts at: 2632 2694 3178 4563 6135 2632
Size: 62 484 1385 1572 3317
Fragments arranged by size:
3317 1572 1385 484 62
ApaI G_GGCC'C
Cuts at: 3557 3557
Size: 6820
ApaLI G'TGCA_C
Cuts at: 4877 6033 4877
Size: 1156 5664
ApoI r'AATT_y
Cuts at: 1493 2194 2298 2347 2419 2762 3035 3406 4129
Size: 701 104 49 72 343 273 371 723
Cuts at: 4129 4355 4507 6616 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 723 701 371 343 273 226
152 104 72 49
AseI AT'TA_AT
Cuts at: 160 1032 5374 160
Size: 872 4342 1606
Fragments arranged by size:
4342 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6820
AvaI C'yCGr_G
Cuts at: 1870 1943 2733 2846 3539 4145 1870
Size: 73 790 113 693 606 4545
Fragments arranged by size:
4545 790 693 606 113 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3724 3757 3724
Size: 33 6787
BamHI G'GATC_C
Cuts at: 3166 4014 4150 4517 3166
Size: 848 136 367 5469
Fragments arranged by size:
5469 848 367 136
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2441 2867 4141 5596 618
Size: 325 217 627 654 426 1274 1455 1842
Fragments arranged by size:
1842 1455 1274 654 627 426 325 217
BanII G_rGCy'C
Cuts at: 1391 2077 3557 3564 3986 4139 1391
Size: 686 1480 7 422 153 4072
Fragments arranged by size:
4072 1480 686 422 153 7
BbeI G_GCGC'C
Cuts at: 2445 2445
Size: 6820
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2970 928
Size: 971 1071 4778
Fragments arranged by size:
4778 1071 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5011 5045 1903
Size: 34 50 34 2990 34 3678
Fragments arranged by size:
3678 2990 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3752 4712 5922 3752
Size: 960 1210 4650
Fragments arranged by size:
4650 1210 960
BclI T'GATC_A
Cuts at: 1548 4095 1548
Size: 2547 4273
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2939 5431 136
Size: 107 122 71 631 605 1267 2492 1525
Fragments arranged by size:
2492 1525 1267 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6815 6815
Size: 6820
BlpI GC'TnA_GC
Cuts at: 4237 4237
Size: 6820
Bme1580I G_kGCm'C
Cuts at: 1790 2132 2653 3557 3754 4881 6037 1790
Size: 342 521 904 197 1127 1156 2573
Fragments arranged by size:
2573 1156 1127 904 521 342 197
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6820
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2682 4551 5509
Size: 416 53 83 399 24 1265 1869 958
Cuts at: 5509 442
Size: 1753
Fragments arranged by size:
1869 1753 1265 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6820
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2091 2091
Size: 6820
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2099 2141 3650 5480 1239
Size: 860 42 1509 1830 2579
Fragments arranged by size:
2579 1830 1509 860 42
Bpu10I CC'TnA_GC
Cuts at: 2359 3993 4006 2359
Size: 1634 13 5173
Fragments arranged by size:
5173 1634 13
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3347 4945 5810 6108 3347
Size: 1598 865 298 4059
Fragments arranged by size:
4059 1598 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5483 882
Size: 617 3984 2219
Fragments arranged by size:
3984 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2781 2826 4611 493
Size: 711 1577 45 1785 2702
Fragments arranged by size:
2702 1785 1577 711 45
BsaBI GATnn'nnATC
Cuts at: 6814 6814
Size: 6820
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2442 2970 3840 4627
Size: 53 83 186 936 909 528 870 787
Cuts at: 4627 5009 5644 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 909 870 787 635 528 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 2393 3455 5253 5651 5925 6072 6483 2393
Size: 1062 1798 398 274 147 411 2730
Fragments arranged by size:
2730 1798 1062 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3035 3065 3077 3107 3139
Size: 30 165 30 1632 30 12 30 32
Cuts at: 3139 3169 1178
Size: 30 4829
Fragments arranged by size:
4829 1632 165 32 30 30 30 30
30 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2630 3147 3150 4099 1186
Size: 1444 517 3 949 3907
Fragments arranged by size:
3907 1444 949 517 3
BseYI C'CCAG_C
Cuts at: 2601 2652 3549 3985 4045 6023 6519 2601
Size: 51 897 436 60 1978 496 2902
Fragments arranged by size:
2902 1978 897 496 436 60 51
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2234 2628 2707 3073 3700 3859 1163
Size: 573 498 394 79 366 627 159 4124
Fragments arranged by size:
4124 627 573 498 394 366 159 79
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2025 4270 5031 6059 1540
Size: 275 186 24 2245 761 1028 2301
Fragments arranged by size:
2301 2245 1028 761 275 186 24
BsiHKAI G_wGCw'C
Cuts at: 2663 3536 4881 4966 6037 2663
Size: 873 1345 85 1071 3446
Fragments arranged by size:
3446 1345 1071 873 85
BsmI GAATG_Cn'
Cuts at: 3611 4331 4424 3611
Size: 720 93 6007
Fragments arranged by size:
6007 720 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2763 3580 2763
Size: 817 6003
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2077 2132 2653 2663 3536 3557 3564
Size: 399 287 55 521 10 873 21 7
Cuts at: 3564 3754 3986 4139 4881 4966 6037 1391
Size: 190 232 153 742 85 1071 2174
Fragments arranged by size:
2174 1071 873 742 521 399 287 232
190 153 85 55 21 10 7
BspEI T'CCGG_A
Cuts at: 6483 6483
Size: 6820
BspHI T'CATG_A
Cuts at: 4709 6439 4709
Size: 1730 5090
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2322 2801 2882 3722 3794 4175 844
Size: 1478 479 81 840 72 381 3489
Fragments arranged by size:
3489 1478 840 479 381 81 72
BsrBI CCG'CTC
Cuts at: 1394 1823 3978 4707 1394
Size: 429 2155 729 3507
Fragments arranged by size:
3507 2155 729 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2274 5315 5489 65
Size: 1183 1026 3041 174 1396
Fragments arranged by size:
3041 1396 1183 1026 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3121 3842 5464 5651 1700
Size: 110 1311 721 1622 187 2869
Fragments arranged by size:
2869 1622 1311 721 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6820
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6820
BssSI C'ACGA_G
Cuts at: 2104 2920 4880 5892 2104
Size: 816 1960 1012 3032
Fragments arranged by size:
3032 1960 1012 816
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3606 3675 794
Size: 2812 69 3939
Fragments arranged by size:
3939 2812 69
BstBI TT'CG_AA
Cuts at: 5624 5624
Size: 6820
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6820
BstYI r'GATC_y
Cuts at: 1972 3166 4014 4150 4517 4902 4919 6371 6457
Size: 1194 848 136 367 385 17 1452 86
Cuts at: 6457 6469 6815 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1194 848 385 367 346 136
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3696 3696
Size: 6820
BtgI C'CryG_G
Cuts at: 513 1065 2674 513
Size: 552 1609 4659
Fragments arranged by size:
4659 1609 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6081
Fragments arranged by size:
6081 683 56
BtsI GCAGTG_nn'
Cuts at: 2518 2663 3772 4336 5130 5150 2518
Size: 145 1109 564 794 20 4188
Fragments arranged by size:
4188 1109 794 564 145 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6820
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6785
DraI TTT'AAA
Cuts at: 3040 3928 4124 3040
Size: 888 196 5736
Fragments arranged by size:
5736 888 196
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2750 5827 809
Size: 507 1434 3077 1802
Fragments arranged by size:
3077 1802 1434 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2022 3844 4267
Size: 54 1425 50 275 18 192 1822 423
Cuts at: 4267 5156 6785 8
Size: 889 1629 43
Fragments arranged by size:
1822 1629 1425 889 423 275 192 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2022 4267 1537
Size: 275 210 2245 4090
Fragments arranged by size:
4090 2245 275 210
EarI CTCTTCn'nnn_
Cuts at: 1353 4029 4750 1353
Size: 2676 721 3423
Fragments arranged by size:
3423 2676 721
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5407 5781 5927 387
Size: 1064 138 3818 374 146 1280
Fragments arranged by size:
3818 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2099 2141 2420 3650 4883 5480 6267
Size: 393 467 42 279 1230 1233 597 787
Cuts at: 6267 6535 1239
Size: 268 1524
Fragments arranged by size:
1524 1233 1230 787 597 467 393 279
268 42
EcoNI CCTnn'n_nnAGG
Cuts at: 3629 3695 3629
Size: 66 6754
EcoO109I rG'GnC_Cy
Cuts at: 2729 2996 3450 3553 2729
Size: 267 454 103 5996
Fragments arranged by size:
5996 454 267 103
EcoRI G'AATT_C
Cuts at: 2298 4129 4507 2298
Size: 1831 378 4611
Fragments arranged by size:
4611 1831 378
FspI TGC'GCA
Cuts at: 5326 5326
Size: 6820
HaeII r_GCGC'y
Cuts at: 1678 2036 2045 2445 2671 5967 1678
Size: 358 9 400 226 3296 2531
Fragments arranged by size:
3296 2531 400 358 226 9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2903 2935 3035 3067 3442
Size: 32 135 32 1331 32 100 32 375
Cuts at: 3442 3474 3560 3592 4006 4038 5461 5493 5535
Size: 32 86 32 414 32 1423 32 42
Cuts at: 5535 5567 1373
Size: 32 2626
Fragments arranged by size:
2626 1423 1331 414 375 135 100 86
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4164 4410 669
Size: 988 281 2226 246 3079
Fragments arranged by size:
3079 2226 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6820
HpaI GTT'AAC
Cuts at: 4410 4410
Size: 6820
KasI G'GCGC_C
Cuts at: 2441 2441
Size: 6820
KpnI G_GTAC'C
Cuts at: 4145 4145
Size: 6820
MfeI C'AATT_G
Cuts at: 4419 4419
Size: 6820
MluI A'CGCG_T
Cuts at: 6696 6696
Size: 6820
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5934 6118 1305
Size: 4629 184 2007
Fragments arranged by size:
4629 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4998
Fragments arranged by size:
4998 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2534 2876 4071 4778 5137 5296 518
Size: 648 1368 342 1195 707 359 159 2042
Fragments arranged by size:
2042 1368 1195 707 648 359 342 159
MspA1I CmG'CkG
Cuts at: 1606 2562 3498 3989 4045 4236 4913 6061 6306
Size: 956 936 491 56 191 677 1148 245
Cuts at: 6306 1606
Size: 2120
Fragments arranged by size:
2120 1148 956 936 677 491 245 191
56
NaeI GCC'GGC
Cuts at: 3844 3844
Size: 6820
NarI GG'CG_CC
Cuts at: 2442 2442
Size: 6820
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6268
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6820
NgoMIV G'CCGG_C
Cuts at: 3842 3842
Size: 6820
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6820
NotI GC'GGCC_GC
Cuts at: 4267 4267
Size: 6820
NspI r_CATG'y
Cuts at: 1132 1229 2533 4178 5723 1132
Size: 97 1304 1645 1545 2229
Fragments arranged by size:
2229 1645 1545 1304 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6820
PciI A'CATG_T
Cuts at: 2529 5719 2529
Size: 3190 3630
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2257 1108
Size: 1149 5671
PfoI T'CCnGG_A
Cuts at: 2015 2250 2015
Size: 235 6585
PmeI GTTT'AAAC
Cuts at: 4124 4124
Size: 6820
PmlI CAC'GTG
Cuts at: 2781 2781
Size: 6820
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4889 4921 6402 6434 4889
Size: 32 1481 32 5275
Fragments arranged by size:
5275 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3450 3450
Size: 6820
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6820
PsiI TTA'TAA
Cuts at: 3476 4390 3476
Size: 914 5906
PspOMI G'GGCC_C
Cuts at: 3553 3553
Size: 6820
PstI C_TGCA'G
Cuts at: 830 2319 2719 4172 830
Size: 1489 400 1453 3478
Fragments arranged by size:
3478 1489 1453 400
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6820
PvuII CAG'CTG
Cuts at: 2562 3498 3989 4045 2562
Size: 936 491 56 5337
Fragments arranged by size:
5337 936 491 56
SalI G'TCGA_C
Cuts at: 1936 4162 1936
Size: 2226 4594
SbfI CC_TGCA'GG
Cuts at: 4172 4172
Size: 6820
ScaI AGT'ACT
Cuts at: 5068 6780 5068
Size: 1712 5108
SfcI C'TryA_G
Cuts at: 826 1045 2315 2715 4168 5303 5984 6175 826
Size: 219 1270 400 1453 1135 681 191 1471
Fragments arranged by size:
1471 1453 1270 1135 681 400 219 191
SfoI GGC'GCC
Cuts at: 2443 2443
Size: 6820
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6820
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6820
SmaI CCC'GGG
Cuts at: 1872 2735 4147 1872
Size: 863 1412 4545
Fragments arranged by size:
4545 1412 863
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3362 4924 5825 6087 820
Size: 197 926 1419 1562 901 262 1553
Fragments arranged by size:
1562 1553 1419 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4611 493
Size: 4118 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6820
SphI G_CATG'C
Cuts at: 1132 4178 1132
Size: 3046 3774
SspI AAT'ATT
Cuts at: 5 52 3070 4744 5
Size: 47 3018 1674 2081
Fragments arranged by size:
3018 2081 1674 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6820
StyI C'CwwG_G
Cuts at: 513 1065 2098 2188 3345 3564 3641 4259 513
Size: 552 1033 90 1157 219 77 618 3074
Fragments arranged by size:
3074 1157 1033 618 552 219 90 77
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3696 4847 4864 5017 5202 5647 325
Size: 1255 2116 1151 17 153 185 445 1498
Fragments arranged by size:
2116 1498 1255 1151 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2337 5066 6778
Size: 274 80 33 51 1443 360 2729 1712
Cuts at: 6778 96
Size: 138
Fragments arranged by size:
2729 1712 1443 360 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3139 3171 6780 6812 3139
Size: 32 3609 32 3147
Fragments arranged by size:
3609 3147 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2504 2969 3572 1316
Size: 1188 465 603 4564
Fragments arranged by size:
4564 1188 603 465
XbaI T'CTAG_A
Cuts at: 4156 4156
Size: 6820
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2254 2258 2830 2254
Size: 4 572 6244
Fragments arranged by size:
6244 572 4
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6820
XmaI C'CCGG_G
Cuts at: 1870 2733 4145 1870
Size: 863 1412 4545
Fragments arranged by size:
4545 1412 863
XmnI GAAnn'nnTTC
Cuts at: 4949 4949
Size: 6820
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4628 276
Size: 53 83 186 936 3094 2468
Fragments arranged by size:
3094 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AscI AvrII BbvCI BfrBI BsiWI BstXI BstZ17I DraIII
EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI
PspXI PsrI RsrII SacI SacII SanDI SapI SexAI
SfiI SrfI SwaI