Restriction map for
pFN21AE2771
(Circular) (Six-base) MAPSORT of: pFN21AE2771.seq Check: 4849 from: 1 to: 6232
pFN21AE2771 6232 bp
With 182 enzymes: SgfI *
July 4, 2020 18:17 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4042 278
Size: 53 83 186 936 2506 2468
Fragments arranged by size:
2506 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3575 1937
Size: 1638 4594
Acc65I G'GTAC_C
Cuts at: 3553 3553
Size: 6232
AclI AA'CG_TT
Cuts at: 1563 4359 4732 1563
Size: 2796 373 3063
Fragments arranged by size:
3063 2796 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2239 4295 5679 5947 1632
Size: 607 2056 1384 268 1917
Fragments arranged by size:
2056 1917 1384 607 268
AfeI AGC'GCT
Cuts at: 2421 2421
Size: 6232
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6035
AflIII A'CryG_T
Cuts at: 1203 5131 6108 1203
Size: 3928 977 1327
Fragments arranged by size:
3928 1327 977
AgeI A'CCGG_T
Cuts at: 5063 5063
Size: 6232
AhdI GACnn_n'nnGTC
Cuts at: 4961 4961
Size: 6232
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6232
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6232
AlwNI CAG_nnn'CTG
Cuts at: 2217 3975 5547 2217
Size: 1758 1572 2902
Fragments arranged by size:
2902 1758 1572
ApaLI G'TGCA_C
Cuts at: 4289 5445 4289
Size: 1156 5076
ApoI r'AATT_y
Cuts at: 1493 3541 3767 3919 6028 1493
Size: 2048 226 152 2109 1697
Fragments arranged by size:
2109 2048 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3111 3308 4786 160
Size: 872 2079 197 1478 1606
Fragments arranged by size:
2079 1606 1478 872 197
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6232
AvaI C'yCGr_G
Cuts at: 1870 1943 3557 1870
Size: 73 1614 4545
Fragments arranged by size:
4545 1614 73
AvrII C'CTAG_G
Cuts at: 3217 3337 3217
Size: 120 6112
BamHI G'GATC_C
Cuts at: 2087 2168 3213 3562 3929 2087
Size: 81 1045 349 367 4390
Fragments arranged by size:
4390 1045 367 349 81
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3553 5008 618
Size: 325 217 627 1766 1455 1842
Fragments arranged by size:
1842 1766 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3551 1391
Size: 2160 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3272 928
Size: 971 1373 3888
Fragments arranged by size:
3888 1373 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4423 4457 1903
Size: 34 2486 34 3678
Fragments arranged by size:
3678 2486 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3081 3457 4124 5334 3081
Size: 376 667 1210 3979
Fragments arranged by size:
3979 1210 667 376
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6232
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4843 136
Size: 107 122 71 631 605 3171 1525
Fragments arranged by size:
3171 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2620 6227 2620
Size: 3607 2625
BlpI GC'TnA_GC
Cuts at: 3649 3649
Size: 6232
Bme1580I G_kGCm'C
Cuts at: 1790 4293 5449 1790
Size: 2503 1156 2573
Fragments arranged by size:
2573 2503 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6232
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3963 4921 442
Size: 416 53 83 399 24 2546 958 1753
Fragments arranged by size:
2546 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6232
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2295 2598 4892 1239
Size: 1056 303 2294 2579
Fragments arranged by size:
2579 2294 1056 303
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4357 5222 5520 4357
Size: 865 298 5069
Fragments arranged by size:
5069 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2563 4895 882
Size: 617 1064 2332 2219
Fragments arranged by size:
2332 2219 1064 617
BsaAI yAC'GTr
Cuts at: 493 1204 2388 4023 493
Size: 711 1184 1635 2702
Fragments arranged by size:
2702 1635 1184 711
BsaBI GATnn'nnATC
Cuts at: 6226 6226
Size: 6232
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4039 4421 5056 275
Size: 53 83 186 936 2506 382 635 1451
Fragments arranged by size:
2506 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4665 5063 5337 5484 5895 4665
Size: 398 274 147 411 5002
Fragments arranged by size:
5002 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6007
Fragments arranged by size:
6007 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6232
BseYI C'CCAG_C
Cuts at: 2040 3421 5435 5931 2040
Size: 1381 2014 496 2341
Fragments arranged by size:
2341 2014 1381 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5659
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2407 3682 4443 5471 1540
Size: 275 186 406 1275 761 1028 2301
Fragments arranged by size:
2301 1275 1028 761 406 275 186
BsiHKAI G_wGCw'C
Cuts at: 4293 4378 5449 4293
Size: 85 1071 5076
Fragments arranged by size:
5076 1071 85
BsmI GAATG_Cn'
Cuts at: 3743 3836 3743
Size: 93 6139
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3551 4293 4378 5449 1391
Size: 399 1761 742 85 1071 2174
Fragments arranged by size:
2174 1761 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5895 5895
Size: 6232
BspHI T'CATG_A
Cuts at: 4121 5851 4121
Size: 1730 4502
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3587 844
Size: 2743 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4119 1394
Size: 429 2296 3507
Fragments arranged by size:
3507 2296 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2934 4727 4901 65
Size: 1183 1686 1793 174 1396
Fragments arranged by size:
1793 1686 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4876 5063 1700
Size: 110 3066 187 2869
Fragments arranged by size:
3066 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2112 3510 96
Size: 2016 1398 2818
Fragments arranged by size:
2818 2016 1398
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6232
BssSI C'ACGA_G
Cuts at: 4292 5304 4292
Size: 1012 5220
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2482 794
Size: 1688 4544
BstBI TT'CG_AA
Cuts at: 5036 5036
Size: 6232
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6232
BstXI CCAn_nnnn'nTGG
Cuts at: 3299 3299
Size: 6232
BstYI r'GATC_y
Cuts at: 1972 2087 2168 2620 3213 3562 3929 4314 4331
Size: 115 81 452 593 349 367 385 17
Cuts at: 4331 5783 5869 5881 6227 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 593 452 385 367 349 346
115 86 81 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4741
Fragments arranged by size:
4741 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5493
Fragments arranged by size:
5493 683 56
BtsI GCAGTG_nn'
Cuts at: 2367 3748 4542 4562 2367
Size: 1381 794 20 4037
Fragments arranged by size:
4037 1381 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6232
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6197
DraI TTT'AAA
Cuts at: 2541 2908 3536 2541
Size: 367 628 5237
Fragments arranged by size:
5237 628 367
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5239 809
Size: 507 3923 1802
Fragments arranged by size:
3923 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3679 4568 6197
Size: 54 1425 50 275 18 1849 889 1629
Cuts at: 6197 8
Size: 43
Fragments arranged by size:
1849 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3679 1537
Size: 275 1867 4090
Fragments arranged by size:
4090 1867 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3015 4162 1353
Size: 1662 1147 3423
Fragments arranged by size:
3423 1662 1147
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4819 5193 5339 387
Size: 1064 138 3230 374 146 1280
Fragments arranged by size:
3230 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2239 2295 2598 4295 4892 5679 5947
Size: 393 607 56 303 1697 597 787 268
Cuts at: 5947 1239
Size: 1524
Fragments arranged by size:
1697 1524 787 607 597 393 303 268
56
EcoRI G'AATT_C
Cuts at: 3541 3919 3541
Size: 378 5854
FspI TGC'GCA
Cuts at: 4738 4738
Size: 6232
HaeII r_GCGC'y
Cuts at: 1678 2423 5379 1678
Size: 745 2956 2531
Fragments arranged by size:
2956 2531 745
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2621 2653 3333 3365 4873
Size: 32 135 32 1049 32 680 32 1508
Cuts at: 4873 4905 4947 4979 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1508 1049 680 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3576 3822 669
Size: 988 281 1638 246 3079
Fragments arranged by size:
3079 1638 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6232
HpaI GTT'AAC
Cuts at: 3822 3822
Size: 6232
KpnI G_GTAC'C
Cuts at: 3557 3557
Size: 6232
MfeI C'AATT_G
Cuts at: 3831 3831
Size: 6232
MluI A'CGCG_T
Cuts at: 6108 6108
Size: 6232
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2083 2146 3521 5346 5530 1305
Size: 778 63 1375 1825 184 2007
Fragments arranged by size:
2007 1825 1375 778 184 63
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4410
Fragments arranged by size:
4410 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3297 4190 4549 4708 518
Size: 648 2131 893 359 159 2042
Fragments arranged by size:
2131 2042 893 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3648 4325 5473 5718 1606
Size: 2042 677 1148 245 2120
Fragments arranged by size:
2120 2042 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4741
Fragments arranged by size:
4741 939 552
NdeI CA'TA_TG
Cuts at: 387 3253 387
Size: 2866 3366
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6232
NotI GC'GGCC_GC
Cuts at: 3679 3679
Size: 6232
NspI r_CATG'y
Cuts at: 1132 1229 3471 3590 5135 1132
Size: 97 2242 119 1545 2229
Fragments arranged by size:
2242 2229 1545 119 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6232
PciI A'CATG_T
Cuts at: 5131 5131
Size: 6232
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6232
PmeI GTTT'AAAC
Cuts at: 3536 3536
Size: 6232
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4301 4333 5814 5846 4301
Size: 32 1481 32 4687
Fragments arranged by size:
4687 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6232
PsiI TTA'TAA
Cuts at: 3802 3802
Size: 6232
PstI C_TGCA'G
Cuts at: 830 2213 2478 3584 830
Size: 1383 265 1106 3478
Fragments arranged by size:
3478 1383 1106 265
PvuI CG_AT'CG
Cuts at: 2001 2407 2001
Size: 406 5826
SalI G'TCGA_C
Cuts at: 1936 3574 1936
Size: 1638 4594
SbfI CC_TGCA'GG
Cuts at: 3584 3584
Size: 6232
ScaI AGT'ACT
Cuts at: 4480 6192 4480
Size: 1712 4520
SfcI C'TryA_G
Cuts at: 826 1045 2209 2474 2698 3580 4715 5396 5587
Size: 219 1164 265 224 882 1135 681 191
Cuts at: 5587 826
Size: 1471
Fragments arranged by size:
1471 1164 1135 882 681 265 224 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6232
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6232
SmaI CCC'GGG
Cuts at: 1872 3559 1872
Size: 1687 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4336 5237 5499 820
Size: 197 926 2393 901 262 1553
Fragments arranged by size:
2393 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4023 493
Size: 3530 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6232
SphI G_CATG'C
Cuts at: 1132 3590 1132
Size: 2458 3774
SspI AAT'ATT
Cuts at: 5 52 4156 5
Size: 47 4104 2081
Fragments arranged by size:
4104 2081 47
StuI AGG'CCT
Cuts at: 1517 2280 2415 1517
Size: 763 135 5334
Fragments arranged by size:
5334 763 135
StyI C'CwwG_G
Cuts at: 513 1065 2004 3217 3337 3671 513
Size: 552 939 1213 120 334 3074
Fragments arranged by size:
3074 1213 939 552 334 120
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2032 2516 3361 4259 4276 4429 4614
Size: 1255 452 484 845 898 17 153 185
Cuts at: 4614 5059 325
Size: 445 1498
Fragments arranged by size:
1498 1255 898 845 484 452 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2112 3510 4478
Size: 274 80 33 51 1443 135 1398 968
Cuts at: 4478 6190 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1398 968 274 138 135 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6192 6224 6192
Size: 32 6200
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6232
XbaI T'CTAG_A
Cuts at: 3568 3568
Size: 6232
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3268 3268
Size: 6232
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6232
XmaI C'CCGG_G
Cuts at: 1870 3557 1870
Size: 1687 4545
XmnI GAAnn'nnTTC
Cuts at: 4361 4361
Size: 6232
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4040 276
Size: 53 83 186 936 2506 2468
Fragments arranged by size:
2506 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI