Restriction map for
pFN21AE2769
(Circular) (Six-base) MAPSORT of: pFN21AE2769.seq Check: 2380 from: 1 to: 6118
pFN21AE2769 6118 bp
With 182 enzymes: SgfI *
July 4, 2020 18:17 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3928 278
Size: 53 83 186 936 2392 2468
Fragments arranged by size:
2468 2392 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3461 1937
Size: 1524 4594
Acc65I G'GTAC_C
Cuts at: 3361 3439 3361
Size: 78 6040
AclI AA'CG_TT
Cuts at: 1563 4245 4618 1563
Size: 2682 373 3063
Fragments arranged by size:
3063 2682 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2777 2813 4181 5565 5833 1632
Size: 1145 36 1368 1384 268 1917
Fragments arranged by size:
1917 1384 1368 1145 268 36
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5921
AflIII A'CryG_T
Cuts at: 1203 3168 5017 5994 1203
Size: 1965 1849 977 1327
Fragments arranged by size:
1965 1849 1327 977
AgeI A'CCGG_T
Cuts at: 4949 4949
Size: 6118
AhdI GACnn_n'nnGTC
Cuts at: 4847 4847
Size: 6118
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6118
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6118
AlwNI CAG_nnn'CTG
Cuts at: 2179 3052 3861 5433 2179
Size: 873 809 1572 2864
Fragments arranged by size:
2864 1572 873 809
ApaLI G'TGCA_C
Cuts at: 4175 5331 4175
Size: 1156 4962
ApoI r'AATT_y
Cuts at: 1493 3427 3653 3805 5914 1493
Size: 1934 226 152 2109 1697
Fragments arranged by size:
2109 1934 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4672 160
Size: 872 3640 1606
Fragments arranged by size:
3640 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6118
AvaI C'yCGr_G
Cuts at: 1870 1943 3443 1870
Size: 73 1500 4545
Fragments arranged by size:
4545 1500 73
BamHI G'GATC_C
Cuts at: 3448 3815 3448
Size: 367 5751
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2031 2200 2323 2347 3361
Size: 325 217 627 244 169 123 24 1014
Cuts at: 3361 3439 4894 618
Size: 78 1455 1842
Fragments arranged by size:
1842 1455 1014 627 325 244 217 169
123 78 24
BanII G_rGCy'C
Cuts at: 1391 2384 2732 2994 3190 3437 1391
Size: 993 348 262 196 247 4072
Fragments arranged by size:
4072 993 348 262 247 196
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2071 928
Size: 971 172 4975
Fragments arranged by size:
4975 971 172
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4309 4343 1903
Size: 34 2372 34 3678
Fragments arranged by size:
3678 2372 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4010 5220 4010
Size: 1210 4908
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6118
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2134 2321 4729
Size: 107 122 71 631 605 462 187 2408
Cuts at: 4729 136
Size: 1525
Fragments arranged by size:
2408 1525 631 605 462 187 122 107
71
BglII A'GATC_T
Cuts at: 6113 6113
Size: 6118
BlpI GC'TnA_GC
Cuts at: 3535 3535
Size: 6118
Bme1580I G_kGCm'C
Cuts at: 1790 2352 3042 4179 5335 1790
Size: 562 690 1137 1156 2573
Fragments arranged by size:
2573 1156 1137 690 562
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6118
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3256 3849 4807
Size: 416 53 83 399 24 1839 593 958
Cuts at: 4807 442
Size: 1753
Fragments arranged by size:
1839 1753 958 593 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6118
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3204 3204
Size: 6118
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2518 2867 2908 3018 4778 1239
Size: 1279 349 41 110 1760 2579
Fragments arranged by size:
2579 1760 1279 349 110 41
Bpu10I CC'TnA_GC
Cuts at: 2036 2036
Size: 6118
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4243 5108 5406 4243
Size: 865 298 4955
Fragments arranged by size:
4955 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2172 4781 882
Size: 617 673 2609 2219
Fragments arranged by size:
2609 2219 673 617
BsaAI yAC'GTr
Cuts at: 493 1204 3169 3909 493
Size: 711 1965 740 2702
Fragments arranged by size:
2702 1965 740 711
BsaBI GATnn'nnATC
Cuts at: 6112 6112
Size: 6118
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2046 3925 4307 4942
Size: 53 83 186 936 513 1879 382 635
Cuts at: 4942 275
Size: 1451
Fragments arranged by size:
1879 1451 936 635 513 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2294 3084 4551 4949 5223 5370 5781 2294
Size: 790 1467 398 274 147 411 2631
Fragments arranged by size:
2631 1467 790 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5893
Fragments arranged by size:
5893 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2348 1186
Size: 1162 4956
BseYI C'CCAG_C
Cuts at: 2274 3042 3074 5321 5817 2274
Size: 768 32 2247 496 2575
Fragments arranged by size:
2575 2247 768 496 32
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3029 3333 3357 1163
Size: 573 1293 304 24 3924
Fragments arranged by size:
3924 1293 573 304 24
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3568 4329 5357 1540
Size: 275 186 1567 761 1028 2301
Fragments arranged by size:
2301 1567 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2994 3190 4179 4264 5335 2994
Size: 196 989 85 1071 3777
Fragments arranged by size:
3777 1071 989 196 85
BsmI GAATG_Cn'
Cuts at: 2616 3629 3722 2616
Size: 1013 93 5012
Fragments arranged by size:
5012 1013 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2352 2384 2732 2994 3042 3190 3437
Size: 399 562 32 348 262 48 148 247
Cuts at: 3437 4179 4264 5335 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 562 399 348 262 247
148 85 48 32
BspEI T'CCGG_A
Cuts at: 5781 5781
Size: 6118
BspHI T'CATG_A
Cuts at: 4007 5737 4007
Size: 1730 4388
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3473 844
Size: 2629 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3183 4005 1394
Size: 429 1360 822 3507
Fragments arranged by size:
3507 1360 822 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4613 4787 65
Size: 1183 3365 174 1396
Fragments arranged by size:
3365 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4762 4949 1700
Size: 110 2952 187 2869
Fragments arranged by size:
2952 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6118
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6118
BssSI C'ACGA_G
Cuts at: 4178 5190 4178
Size: 1012 5106
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2611 3052 794
Size: 1817 441 3860
Fragments arranged by size:
3860 1817 441
BstBI TT'CG_AA
Cuts at: 2814 4922 2814
Size: 2108 4010
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6118
BstYI r'GATC_y
Cuts at: 1972 3448 3815 4200 4217 5669 5755 5767 6113
Size: 1476 367 385 17 1452 86 12 346
Cuts at: 6113 1972
Size: 1977
Fragments arranged by size:
1977 1476 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2682 2697 2682
Size: 15 6103
BtgI C'CryG_G
Cuts at: 513 1065 2400 3102 513
Size: 552 1335 702 3529
Fragments arranged by size:
3529 1335 702 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5379
Fragments arranged by size:
5379 683 56
BtsI GCAGTG_nn'
Cuts at: 2637 3634 4428 4448 2637
Size: 997 794 20 4307
Fragments arranged by size:
4307 997 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6118
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6083
DraI TTT'AAA
Cuts at: 3422 3422
Size: 6118
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5125 809
Size: 507 3809 1802
Fragments arranged by size:
3809 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3060 3565 4454
Size: 54 1425 50 275 18 1230 505 889
Cuts at: 4454 6083 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1230 889 505 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3565 1537
Size: 275 1753 4090
Fragments arranged by size:
4090 1753 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2093 2372 2468 2918 4048 1353
Size: 740 279 96 450 1130 3423
Fragments arranged by size:
3423 1130 740 450 279 96
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4705 5079 5225 387
Size: 1064 138 3116 374 146 1280
Fragments arranged by size:
3116 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2992 3188 2992
Size: 196 5922
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2518 2777 2813 2867 2908 3018 4181
Size: 393 886 259 36 54 41 110 1163
Cuts at: 4181 4778 5565 5833 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1163 886 787 597 393 268 259
110 54 41 36
EcoNI CCTnn'n_nnAGG
Cuts at: 2449 2449
Size: 6118
EcoO109I rG'GnC_Cy
Cuts at: 2010 2010
Size: 6118
EcoRI G'AATT_C
Cuts at: 3427 3805 3427
Size: 378 5740
FspI TGC'GCA
Cuts at: 4624 4624
Size: 6118
HaeII r_GCGC'y
Cuts at: 1678 5265 1678
Size: 3587 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2450 2482 3142 3174 4759
Size: 32 135 32 878 32 660 32 1585
Cuts at: 4759 4791 4833 4865 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1585 878 660 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2637 3462 3708 669
Size: 988 281 699 825 246 3079
Fragments arranged by size:
3079 988 825 699 281 246
HindIII A'AGCT_T
Cuts at: 748 3331 748
Size: 2583 3535
HpaI GTT'AAC
Cuts at: 3708 3708
Size: 6118
KpnI G_GTAC'C
Cuts at: 3365 3443 3365
Size: 78 6040
MfeI C'AATT_G
Cuts at: 3717 3717
Size: 6118
MluI A'CGCG_T
Cuts at: 5994 5994
Size: 6118
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5232 5416 1305
Size: 3927 184 2007
Fragments arranged by size:
3927 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3062 10
Size: 54 1425 343 1230 3066
Fragments arranged by size:
3066 1425 1230 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2467 4076 4435 4594 518
Size: 648 1301 1609 359 159 2042
Fragments arranged by size:
2042 1609 1301 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3052 3074 3534 4211 5359 5604 1606
Size: 1446 22 460 677 1148 245 2120
Fragments arranged by size:
2120 1446 1148 677 460 245 22
NcoI C'CATG_G
Cuts at: 513 1065 2400 513
Size: 552 1335 4231
Fragments arranged by size:
4231 1335 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6118
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6118
NotI GC'GGCC_GC
Cuts at: 3565 3565
Size: 6118
NspI r_CATG'y
Cuts at: 1132 1229 3476 5021 1132
Size: 97 2247 1545 2229
Fragments arranged by size:
2247 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6118
PciI A'CATG_T
Cuts at: 5017 5017
Size: 6118
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2978 1108
Size: 1870 4248
PfoI T'CCnGG_A
Cuts at: 2534 2916 2534
Size: 382 5736
PmeI GTTT'AAAC
Cuts at: 3422 3422
Size: 6118
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4187 4219 5700 5732 4187
Size: 32 1481 32 4573
Fragments arranged by size:
4573 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2010 2010
Size: 6118
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6118
PsiI TTA'TAA
Cuts at: 3688 3688
Size: 6118
PstI C_TGCA'G
Cuts at: 830 3470 830
Size: 2640 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6118
PvuII CAG'CTG
Cuts at: 3052 3052
Size: 6118
SacI G_AGCT'C
Cuts at: 2994 3190 2994
Size: 196 5922
SalI G'TCGA_C
Cuts at: 1936 3460 1936
Size: 1524 4594
SapI GCTCTTCn'nnn_
Cuts at: 2372 2468 2372
Size: 96 6022
SbfI CC_TGCA'GG
Cuts at: 3470 3470
Size: 6118
ScaI AGT'ACT
Cuts at: 4366 6078 4366
Size: 1712 4406
SfcI C'TryA_G
Cuts at: 826 1045 2171 3466 4601 5282 5473 826
Size: 219 1126 1295 1135 681 191 1471
Fragments arranged by size:
1471 1295 1135 1126 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6118
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6118
SmaI CCC'GGG
Cuts at: 1872 3445 1872
Size: 1573 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4222 5123 5385 820
Size: 197 926 2279 901 262 1553
Fragments arranged by size:
2279 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3909 493
Size: 3416 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6118
SphI G_CATG'C
Cuts at: 1132 3476 1132
Size: 2344 3774
SspI AAT'ATT
Cuts at: 5 52 4042 5
Size: 47 3990 2081
Fragments arranged by size:
3990 2081 47
StuI AGG'CCT
Cuts at: 1517 2826 1517
Size: 1309 4809
StyI C'CwwG_G
Cuts at: 513 1065 2041 2280 2400 3003 3557 513
Size: 552 976 239 120 603 554 3074
Fragments arranged by size:
3074 976 603 554 552 239 120
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2218 2434 3391 4145 4162 4315 4500
Size: 1255 638 216 957 754 17 153 185
Cuts at: 4500 4945 325
Size: 445 1498
Fragments arranged by size:
1498 1255 957 754 638 445 216 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4364 6076 96
Size: 274 80 33 51 1443 2387 1712 138
Fragments arranged by size:
2387 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6078 6110 6078
Size: 32 6086
Tth111I GACn'n_nGTC
Cuts at: 1316 2542 3262 1316
Size: 1226 720 4172
Fragments arranged by size:
4172 1226 720
XbaI T'CTAG_A
Cuts at: 3454 3454
Size: 6118
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6118
XmaI C'CCGG_G
Cuts at: 1870 3443 1870
Size: 1573 4545
XmnI GAAnn'nnTTC
Cuts at: 3334 4247 3334
Size: 913 5205
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3926 276
Size: 53 83 186 936 2392 2468
Fragments arranged by size:
2468 2392 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SacI SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BsiWI BsmBI BstXI BstZ17I DraIII EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII
SanDI SexAI SfiI SfoI SrfI SwaI XcmI