Restriction map for
pFN21AE2750
(Circular) (Six-base) MAPSORT of: pFN21AE2750.seq Check: 9321 from: 1 to: 10507
pFN21AE2750 10507 bp
With 182 enzymes: SgfI *
June 21, 2019 12:02 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3946 5662 7118 3946
Size: 1716 1456 7335
Fragments arranged by size:
7335 1716 1456
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 8317 278
Size: 53 83 186 936 6781 2468
Fragments arranged by size:
6781 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4202 4803 5856 6654 7328 7850 1937
Size: 2265 601 1053 798 674 522 4594
Fragments arranged by size:
4594 2265 1053 798 674 601 522
Acc65I G'GTAC_C
Cuts at: 7448 7828 7448
Size: 380 10127
AclI AA'CG_TT
Cuts at: 1563 3826 6793 8634 9007 1563
Size: 2263 2967 1841 373 3063
Fragments arranged by size:
3063 2967 2263 1841 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2267 3777 4581 4988 5138 5522 6167 7599
Size: 635 1510 804 407 150 384 645 1432
Cuts at: 7599 8570 9954 10222 1632
Size: 971 1384 268 1917
Fragments arranged by size:
1917 1510 1432 1384 971 804 645 635
407 384 268 150
AfeI AGC'GCT
Cuts at: 2269 2269
Size: 10507
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 10310
AflIII A'CryG_T
Cuts at: 1203 2430 4467 4527 9406 10383 1203
Size: 1227 2037 60 4879 977 1327
Fragments arranged by size:
4879 2037 1327 1227 977 60
AgeI A'CCGG_T
Cuts at: 4667 9338 4667
Size: 4671 5836
AhdI GACnn_n'nnGTC
Cuts at: 3473 6661 9236 3473
Size: 3188 2575 4744
Fragments arranged by size:
4744 3188 2575
AleI CACnn'nnGTG
Cuts at: 1166 2975 4625 4649 4880 1166
Size: 1809 1650 24 231 6793
Fragments arranged by size:
6793 1809 1650 231 24
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2286 2816 3383 1184
Size: 1102 530 567 8308
Fragments arranged by size:
8308 1102 567 530
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6778 6810 6778
Size: 32 10475
AlwNI CAG_nnn'CTG
Cuts at: 2294 4304 5500 6037 6082 6251 7474 8250 9822
Size: 2010 1196 537 45 169 1223 776 1572
Cuts at: 9822 2294
Size: 2979
Fragments arranged by size:
2979 2010 1572 1223 1196 776 537 169
45
ApaI G_GGCC'C
Cuts at: 4607 5330 7673 4607
Size: 723 2343 7441
Fragments arranged by size:
7441 2343 723
ApaLI G'TGCA_C
Cuts at: 8564 9720 8564
Size: 1156 9351
ApoI r'AATT_y
Cuts at: 1493 2749 3422 5829 7816 8042 8194 10303 1493
Size: 1256 673 2407 1987 226 152 2109 1697
Fragments arranged by size:
2407 2109 1987 1697 1256 673 226 152
AseI AT'TA_AT
Cuts at: 160 1032 9061 160
Size: 872 8029 1606
Fragments arranged by size:
8029 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10507
AvaI C'yCGr_G
Cuts at: 1870 1943 2077 2128 3760 4184 6667 7832 1870
Size: 73 134 51 1632 424 2483 1165 4545
Fragments arranged by size:
4545 2483 1632 1165 424 134 73 51
BamHI G'GATC_C
Cuts at: 4544 6937 7837 8204 4544
Size: 2393 900 367 6847
Fragments arranged by size:
6847 2393 900 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2019 3344 4312 4627 6490
Size: 325 217 627 232 1325 968 315 1863
Cuts at: 6490 6881 7339 7448 7828 9283 618
Size: 391 458 109 380 1455 1842
Fragments arranged by size:
1863 1842 1455 1325 968 627 458 391
380 325 315 232 217 109
BanII G_rGCy'C
Cuts at: 1391 2199 2241 3236 4607 4730 5330 5466 6340
Size: 808 42 995 1371 123 600 136 874
Cuts at: 6340 6771 6782 7510 7629 7673 7761 7826 1391
Size: 431 11 728 119 44 88 65 4072
Fragments arranged by size:
4072 1371 995 874 808 728 600 431
136 123 119 88 65 44 42 11
BbeI G_GCGC'C
Cuts at: 2023 2023
Size: 10507
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3795 4266 4401 5763 7266 7710 928
Size: 971 1896 471 135 1362 1503 444 3725
Fragments arranged by size:
3725 1896 1503 1362 971 471 444 135
BbvCI CC'TCA_GC
Cuts at: 3291 4240 7353 7630 3291
Size: 949 3113 277 6168
Fragments arranged by size:
6168 3113 949 277
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3660 3694 7070 7104 8698
Size: 34 50 34 1639 34 3376 34 1594
Cuts at: 8698 8732 1903
Size: 34 3678
Fragments arranged by size:
3678 3376 1639 1594 50 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5170 5432 5484 7002 8399 9609 5170
Size: 262 52 1518 1397 1210 6068
Fragments arranged by size:
6068 1518 1397 1210 262 52
BclI T'GATC_A
Cuts at: 1548 4518 4872 1548
Size: 2970 354 7183
Fragments arranged by size:
7183 2970 354
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2174 7097 7232
Size: 107 122 71 631 605 502 4923 135
Cuts at: 7232 7561 9118 136
Size: 329 1557 1525
Fragments arranged by size:
4923 1557 1525 631 605 502 329 135
122 107 71
BglII A'GATC_T
Cuts at: 5280 10502 5280
Size: 5222 5285
BlpI GC'TnA_GC
Cuts at: 5603 6072 7509 7924 5603
Size: 469 1437 415 8186
Fragments arranged by size:
8186 1437 469 415
Bme1580I G_kGCm'C
Cuts at: 1790 2265 4317 4607 5330 6636 6709 6753 7344
Size: 475 2052 290 723 1306 73 44 591
Cuts at: 7344 7673 8568 9724 1790
Size: 329 895 1156 2573
Fragments arranged by size:
2573 2052 1306 1156 895 723 591 475
329 290 73 44
BmgBI CAC'GTC
Cuts at: 1318 3328 6181 1318
Size: 2010 2853 5644
Fragments arranged by size:
5644 2853 2010
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2268 4056 5438
Size: 416 53 83 399 24 851 1788 1382
Cuts at: 5438 5921 6357 6447 6992 7012 7195 8238 9196
Size: 483 436 90 545 20 183 1043 958
Cuts at: 9196 442
Size: 1753
Fragments arranged by size:
1788 1753 1382 1043 958 851 545 483
436 416 399 183 90 83 53 24
20
BmtI G_CTAG'C
Cuts at: 1056 6441 1056
Size: 5385 5122
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5771 5892 6400 5771
Size: 121 508 9878
Fragments arranged by size:
9878 508 121
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2919 3148 3448 4076 5537 5714 5783 7694
Size: 1680 229 300 628 1461 177 69 1911
Cuts at: 7694 9167 1239
Size: 1473 2579
Fragments arranged by size:
2579 1911 1680 1473 1461 628 300 229
177 69
Bpu10I CC'TnA_GC
Cuts at: 3230 3291 4240 7353 7630 3230
Size: 61 949 3113 277 6107
Fragments arranged by size:
6107 3113 949 277 61
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3423 4097 4289 5290 5428 8632 9497 9795 3423
Size: 674 192 1001 138 3204 865 298 4135
Fragments arranged by size:
4135 3204 1001 865 674 298 192 138
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2929 3750 9170 882
Size: 617 1430 821 5420 2219
Fragments arranged by size:
5420 2219 1430 821 617
BsaAI yAC'GTr
Cuts at: 493 1204 2431 4468 6409 8298 493
Size: 711 1227 2037 1941 1889 2702
Fragments arranged by size:
2702 2037 1941 1889 1227 711
BsaBI GATnn'nnATC
Cuts at: 4866 5706 6716 6931 10501 4866
Size: 840 1010 215 3570 4872
Fragments arranged by size:
4872 3570 1010 840 215
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2020 2026 3599 3650
Size: 53 83 186 936 487 6 1573 51
Cuts at: 3650 4713 5535 6302 6903 7083 8314 8696 9331
Size: 1063 822 767 601 180 1231 382 635
Cuts at: 9331 275
Size: 1451
Fragments arranged by size:
1573 1451 1231 1063 936 822 767 635
601 487 382 186 180 83 53 51
6
BsaWI w'CCGG_w
Cuts at: 2203 4667 5012 5710 5878 8940 9338 9612 9759
Size: 2464 345 698 168 3062 398 274 147
Cuts at: 9759 10170 2203
Size: 411 2540
Fragments arranged by size:
3062 2540 2464 698 411 398 345 274
168 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3001 3031 3064 3094 6404
Size: 30 165 30 1598 30 33 30 3310
Cuts at: 6404 6434 7403 7433 1178
Size: 30 969 30 4252
Fragments arranged by size:
4252 3310 1598 969 165 33 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2954 3087 4740 5307 7061 7424 7714 7751
Size: 1768 133 1653 567 1754 363 290 37
Cuts at: 7751 7769 1186
Size: 18 3924
Fragments arranged by size:
3924 1768 1754 1653 567 363 290 133
37 18
BseYI C'CCAG_C
Cuts at: 2307 2408 5199 5595 5847 6514 6949 7032 7103
Size: 101 2791 396 252 667 435 83 71
Cuts at: 7103 7222 7678 9710 10206 2307
Size: 119 456 2032 496 2608
Fragments arranged by size:
2791 2608 2032 667 496 456 435 396
252 119 101 83 71
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2256 2979 3259 4290 4922 4977 5297
Size: 573 520 723 280 1031 632 55 320
Cuts at: 5297 5690 5781 6980 7811 1163
Size: 393 91 1199 831 3859
Fragments arranged by size:
3859 1199 1031 831 723 632 573 520
393 320 280 91 55
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2092 2174 3583 6523 7957 8718
Size: 275 186 91 82 1409 2940 1434 761
Cuts at: 8718 9746 1540
Size: 1028 2301
Fragments arranged by size:
2940 2301 1434 1409 1028 761 275 186
91 82
BsiHKAI G_wGCw'C
Cuts at: 2199 2241 4456 4658 6340 6408 6594 6782 7510
Size: 42 2215 202 1682 68 186 188 728
Cuts at: 7510 7761 7787 8568 8653 9724 2199
Size: 251 26 781 85 1071 2982
Fragments arranged by size:
2982 2215 1682 1071 781 728 251 202
188 186 85 68 42 26
BsiWI C'GTAC_G
Cuts at: 7569 7569
Size: 10507
BsmI GAATG_Cn'
Cuts at: 3062 3693 8018 8111 3062
Size: 631 4325 93 5458
Fragments arranged by size:
5458 4325 631 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3503 3948 5880 6171 3503
Size: 445 1932 291 7839
Fragments arranged by size:
7839 1932 445 291
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2199 2241 2265 3236 4317 4456 4607
Size: 399 409 42 24 971 1081 139 151
Cuts at: 4607 4658 4730 5330 5466 6340 6408 6594 6636
Size: 51 72 600 136 874 68 186 42
Cuts at: 6636 6709 6753 6771 6782 7344 7510 7629 7673
Size: 73 44 18 11 562 166 119 44
Cuts at: 7673 7761 7787 7826 8568 8653 9724 1391
Size: 88 26 39 742 85 1071 2174
Fragments arranged by size:
2174 1081 1071 971 874 742 600 562
409 399 186 166 151 139 136 119
88 85 73 72 68 51 44 44
42 42 39 26 24 18 11
BspEI T'CCGG_A
Cuts at: 2203 5878 10170 2203
Size: 3675 4292 2540
Fragments arranged by size:
4292 3675 2540
BspHI T'CATG_A
Cuts at: 6118 8396 10126 6118
Size: 2278 1730 6499
Fragments arranged by size:
6499 2278 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3946 4542 5662 6315 7118 7862 844
Size: 3102 596 1120 653 803 744 3489
Fragments arranged by size:
3489 3102 1120 803 744 653 596
BsrBI CCG'CTC
Cuts at: 1394 1823 2088 6348 8394 1394
Size: 429 265 4260 2046 3507
Fragments arranged by size:
4260 3507 2046 429 265
BsrDI GCAATG_nn'
Cuts at: 65 1248 3083 5841 7289 9002 9176 65
Size: 1183 1835 2758 1448 1713 174 1396
Fragments arranged by size:
2758 1835 1713 1448 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2173 3595 4667 6441 6855 9151 9338
Size: 110 363 1422 1072 1774 414 2296 187
Cuts at: 9338 1700
Size: 2869
Fragments arranged by size:
2869 2296 1774 1422 1072 414 363 187
110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 10507
BssHII G'CGCG_C
Cuts at: 1925 6616 1925
Size: 4691 5816
BssSI C'ACGA_G
Cuts at: 2179 2240 3307 7094 8567 9579 2179
Size: 61 1067 3787 1473 1012 3107
Fragments arranged by size:
3787 3107 1473 1067 1012 61
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3059 4304 794
Size: 2265 1245 6997
Fragments arranged by size:
6997 2265 1245
BstBI TT'CG_AA
Cuts at: 9311 9311
Size: 10507
BstEII G'GTnAC_C
Cuts at: 1184 5822 7682 1184
Size: 4638 1860 4009
Fragments arranged by size:
4638 4009 1860
BstXI CCAn_nnnn'nTGG
Cuts at: 3261 4705 5818 3261
Size: 1444 1113 7950
Fragments arranged by size:
7950 1444 1113
BstYI r'GATC_y
Cuts at: 1972 2404 3433 3491 4544 5280 6042 6201 6417
Size: 432 1029 58 1053 736 762 159 216
Cuts at: 6417 6459 6606 6726 6937 7398 7464 7837 8204
Size: 42 147 120 211 461 66 373 367
Cuts at: 8204 8589 8606 10058 10144 10156 10502 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1053 1029 762 736 461 432
385 373 367 346 216 211 159 147
120 86 66 58 42 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2040 2232 2510 2601 3495 3577 4157
Size: 552 975 192 278 91 894 82 580
Cuts at: 4157 4644 6628 6738 7698 513
Size: 487 1984 110 960 3322
Fragments arranged by size:
3322 1984 975 960 894 580 552 487
278 192 110 91 82
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3030 6581 6891 487
Size: 683 56 1804 3551 310 4103
Fragments arranged by size:
4103 3551 1804 683 310 56
BtsI GCAGTG_nn'
Cuts at: 3666 4302 4965 6131 6356 8023 8817 8837 3666
Size: 636 663 1166 225 1667 794 20 5336
Fragments arranged by size:
5336 1667 1166 794 663 636 225 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 10507
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2338 2373 4057 4092 529
Size: 35 1774 35 1684 35 6944
Fragments arranged by size:
6944 1774 1684 35 35 35
DraI TTT'AAA
Cuts at: 4116 7811 4116
Size: 3695 6812
DraIII CAC_nnn'GTG
Cuts at: 3264 6694 7203 3264
Size: 3430 509 6568
Fragments arranged by size:
6568 3430 509
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6260 9514 809
Size: 507 4944 3254 1802
Fragments arranged by size:
4944 3254 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2037 2089 2171
Size: 54 1425 50 275 18 207 52 82
Cuts at: 2171 2454 3303 3580 4887 6012 6741 7233 7717
Size: 283 849 277 1307 1125 729 492 484
Cuts at: 7717 7954 8843 10472 8
Size: 237 889 1629 43
Fragments arranged by size:
1629 1425 1307 1125 889 849 729 492
484 283 277 275 237 207 82 54
52 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2089 2171 3580 7954 1537
Size: 275 277 82 1409 4374 4090
Fragments arranged by size:
4374 4090 1409 277 275 82
EarI CTCTTCn'nnn_
Cuts at: 1353 3925 4062 4888 5861 6226 6264 6770 7179
Size: 2572 137 826 973 365 38 506 409
Cuts at: 7179 7588 8437 1353
Size: 409 849 3423
Fragments arranged by size:
3423 2572 973 849 826 506 409 409
365 137 38
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2092 2668 5641 6545 6599 7360
Size: 1064 138 503 576 2973 904 54 761
Cuts at: 7360 9094 9468 9614 387
Size: 1734 374 146 1280
Fragments arranged by size:
2973 1734 1280 1064 904 761 576 503
374 146 138 54
EcoICRI GAG'CTC
Cuts at: 2197 2239 6338 6780 7508 7759 2197
Size: 42 4099 442 728 251 4945
Fragments arranged by size:
4945 4099 728 442 251 42
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2267 2919 3148 3448 3777 4076 4581
Size: 393 635 652 229 300 329 299 505
Cuts at: 4581 4988 5138 5522 5537 5714 5783 6167 7599
Size: 407 150 384 15 177 69 384 1432
Cuts at: 7599 7694 8570 9167 9954 10222 1239
Size: 95 876 597 787 268 1524
Fragments arranged by size:
1524 1432 876 787 652 635 597 505
407 393 384 384 329 300 299 268
229 177 150 95 69 15
EcoNI CCTnn'n_nnAGG
Cuts at: 2825 6889 7478 2825
Size: 4064 589 5854
Fragments arranged by size:
5854 4064 589
EcoO109I rG'GnC_Cy
Cuts at: 5326 6140 6327 6448 6894 7136 7209 7669 7670
Size: 814 187 121 446 242 73 460 1
Cuts at: 7670 5326
Size: 8163
Fragments arranged by size:
8163 814 460 446 242 187 121 73
1
EcoRI G'AATT_C
Cuts at: 5829 7816 8194 5829
Size: 1987 378 8142
Fragments arranged by size:
8142 1987 378
EcoRV GAT'ATC
Cuts at: 4849 4849
Size: 10507
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4188 6010 4188
Size: 1822 8685
FseI GG_CCGG'CC
Cuts at: 2177 2177
Size: 10507
FspI TGC'GCA
Cuts at: 9013 9013
Size: 10507
HaeII r_GCGC'y
Cuts at: 1678 2023 2271 6131 6508 6628 9654 1678
Size: 345 248 3860 377 120 3026 2531
Fragments arranged by size:
3860 3026 2531 377 345 248 120
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2183 2215 2506 2538 3064
Size: 32 135 32 611 32 291 32 526
Cuts at: 3064 3096 3122 3154 3317 3349 3459 3491 3857
Size: 32 26 32 163 32 110 32 366
Cuts at: 3857 3889 4025 4057 5796 5828 6239 6271 6311
Size: 32 136 32 1739 32 411 32 40
Cuts at: 6311 6343 6404 6436 6647 6650 6679 6682 7403
Size: 32 61 32 211 3 29 3 721
Cuts at: 7403 7435 9148 9180 9222 9254 1373
Size: 32 1713 32 42 32 2626
Fragments arranged by size:
2626 1739 1713 721 611 526 411 366
291 211 163 136 135 110 61 42
40 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 32 29 26 3 3
HincII GTy'rAC
Cuts at: 669 1657 1938 4804 5857 6316 6826 7851 8097
Size: 988 281 2866 1053 459 510 1025 246
Cuts at: 8097 669
Size: 3079
Fragments arranged by size:
3079 2866 1053 1025 988 510 459 281
246
HindIII A'AGCT_T
Cuts at: 748 4165 5066 748
Size: 3417 901 6189
Fragments arranged by size:
6189 3417 901
HpaI GTT'AAC
Cuts at: 8097 8097
Size: 10507
KasI G'GCGC_C
Cuts at: 2019 2019
Size: 10507
KpnI G_GTAC'C
Cuts at: 7452 7832 7452
Size: 380 10127
MfeI C'AATT_G
Cuts at: 8106 8106
Size: 10507
MluI A'CGCG_T
Cuts at: 4527 10383 4527
Size: 5856 4651
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2759 5361 9621 9805 1305
Size: 1454 2602 4260 184 2007
Fragments arranged by size:
4260 2602 2007 1454 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3305 4889 6014 7719 10
Size: 54 1425 343 1473 1584 1125 1705 2798
Fragments arranged by size:
2798 1705 1584 1473 1425 1125 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2417 2975 3365 4124 4625 4649 4703
Size: 648 1251 558 390 759 501 24 54
Cuts at: 4703 4880 5048 5927 8465 8824 8983 518
Size: 177 168 879 2538 359 159 2042
Fragments arranged by size:
2538 2042 1251 879 759 648 558 501
390 359 177 168 159 54 24
MspA1I CmG'CkG
Cuts at: 1606 2109 2663 3898 3909 4051 5599 6630 7923
Size: 503 554 1235 11 142 1548 1031 1293
Cuts at: 7923 8600 9748 9993 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1548 1293 1235 1148 1031 677 554
503 245 142 11
NaeI GCC'GGC
Cuts at: 2175 3597 6443 2175
Size: 1422 2846 6239
Fragments arranged by size:
6239 2846 1422
NarI GG'CG_CC
Cuts at: 2020 2020
Size: 10507
NcoI C'CATG_G
Cuts at: 513 1065 2040 2232 2601 3495 4157 513
Size: 552 975 192 369 894 662 6863
Fragments arranged by size:
6863 975 894 662 552 369 192
NdeI CA'TA_TG
Cuts at: 387 2668 4837 387
Size: 2281 2169 6057
Fragments arranged by size:
6057 2281 2169
NgoMIV G'CCGG_C
Cuts at: 2173 3595 6441 2173
Size: 1422 2846 6239
Fragments arranged by size:
6239 2846 1422
NheI G'CTAG_C
Cuts at: 1052 6437 1052
Size: 5385 5122
NotI GC'GGCC_GC
Cuts at: 7954 7954
Size: 10507
NspI r_CATG'y
Cuts at: 1132 1229 7865 9410 1132
Size: 97 6636 1545 2229
Fragments arranged by size:
6636 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2562 7673 1348
Size: 1214 5111 4182
Fragments arranged by size:
5111 4182 1214
PciI A'CATG_T
Cuts at: 9406 9406
Size: 10507
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2741 7203 7307 1108
Size: 1633 4462 104 4308
Fragments arranged by size:
4462 4308 1633 104
PfoI T'CCnGG_A
Cuts at: 6729 7074 7640 6729
Size: 345 566 9596
Fragments arranged by size:
9596 566 345
PmeI GTTT'AAAC
Cuts at: 7811 7811
Size: 10507
PmlI CAC'GTG
Cuts at: 4468 6409 4468
Size: 1941 8566
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2568 2600 5117 5149 8576 8608 10089 10121 2568
Size: 32 2517 32 3427 32 1481 32 2954
Fragments arranged by size:
3427 2954 2517 1481 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 6140 6327 6448 7209 6140
Size: 187 121 761 9438
Fragments arranged by size:
9438 761 187 121
PshAI GACnn'nnGTC
Cuts at: 1613 4379 1613
Size: 2766 7741
PsiI TTA'TAA
Cuts at: 5057 8077 5057
Size: 3020 7487
PspOMI G'GGCC_C
Cuts at: 4603 5326 7669 4603
Size: 723 2343 7441
Fragments arranged by size:
7441 2343 723
PstI C_TGCA'G
Cuts at: 830 4041 5454 5656 6042 7250 7326 7668 7859
Size: 3211 1413 202 386 1208 76 342 191
Cuts at: 7859 830
Size: 3478
Fragments arranged by size:
3478 3211 1413 1208 386 342 202 191
76
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 10507
PvuII CAG'CTG
Cuts at: 4051 5599 4051
Size: 1548 8959
SacI G_AGCT'C
Cuts at: 2199 2241 6340 6782 7510 7761 2199
Size: 42 4099 442 728 251 4945
Fragments arranged by size:
4945 4099 728 442 251 42
SacII CC_GC'GG
Cuts at: 6631 6631
Size: 10507
SalI G'TCGA_C
Cuts at: 1936 4802 5855 7849 1936
Size: 2866 1053 1994 4594
Fragments arranged by size:
4594 2866 1994 1053
SanDI GG'GwC_CC
Cuts at: 6448 6448
Size: 10507
SapI GCTCTTCn'nnn_
Cuts at: 4888 6770 4888
Size: 1882 8625
SbfI CC_TGCA'GG
Cuts at: 7859 7859
Size: 10507
ScaI AGT'ACT
Cuts at: 4935 5151 8755 10467 4935
Size: 216 3604 1712 4975
Fragments arranged by size:
4975 3604 1712 216
SexAI A'CCwGG_T
Cuts at: 6819 7300 6819
Size: 481 10026
SfcI C'TryA_G
Cuts at: 826 1045 4037 5450 5646 5652 6038 6886 7216
Size: 219 2992 1413 196 6 386 848 330
Cuts at: 7216 7246 7322 7329 7664 7855 8990 9671 9862
Size: 30 76 7 335 191 1135 681 191
Cuts at: 9862 826
Size: 1471
Fragments arranged by size:
2992 1471 1413 1135 848 681 386 335
330 219 196 191 191 76 30 7
6
SfiI GGCCn_nnn'nGGCC
Cuts at: 2174 7561 2174
Size: 5387 5120
SfoI GGC'GCC
Cuts at: 2021 2021
Size: 10507
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10507
SgrAI Cr'CCGG_yG
Cuts at: 1700 4667 1700
Size: 2967 7540
SmaI CCC'GGG
Cuts at: 1872 2079 2130 7834 1872
Size: 207 51 5704 4545
Fragments arranged by size:
5704 4545 207 51
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3438 4076 4184 4268 5305 5407
Size: 197 926 1495 638 108 84 1037 102
Cuts at: 5407 8611 9512 9774 820
Size: 3204 901 262 1553
Fragments arranged by size:
3204 1553 1495 1037 926 901 638 262
197 108 102 84
SnaBI TAC'GTA
Cuts at: 493 8298 493
Size: 7805 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 10507
SphI G_CATG'C
Cuts at: 1132 7865 1132
Size: 6733 3774
SspI AAT'ATT
Cuts at: 5 52 8431 5
Size: 47 8379 2081
Fragments arranged by size:
8379 2081 47
StuI AGG'CCT
Cuts at: 1517 5217 7367 1517
Size: 3700 2150 4657
Fragments arranged by size:
4657 3700 2150
StyI C'CwwG_G
Cuts at: 513 1065 2040 2232 2377 2601 2913 3495 3999
Size: 552 975 192 145 224 312 582 504
Cuts at: 3999 4157 7607 7946 513
Size: 158 3450 339 3074
Fragments arranged by size:
3450 3074 975 582 552 504 339 312
224 192 158 145
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2691 3670 4633 4697 5213 6537 7214
Size: 1255 1111 979 963 64 516 1324 677
Cuts at: 7214 8534 8551 8704 8889 9334 325
Size: 1320 17 153 185 445 1498
Fragments arranged by size:
1498 1324 1320 1255 1111 979 963 677
516 445 185 153 64 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2396 4908 4933
Size: 274 80 33 51 1443 419 2512 25
Cuts at: 4933 5149 7020 8753 10465 96
Size: 216 1871 1733 1712 138
Fragments arranged by size:
2512 1871 1733 1712 1443 419 274 216
138 80 51 33 25
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3550 3582 4669 4701 5430 5462 6483 6515 10467
Size: 32 1087 32 729 32 1021 32 3952
Cuts at: 10467 10499 3550
Size: 32 3558
Fragments arranged by size:
3952 3558 1087 1021 729 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 10507
XbaI T'CTAG_A
Cuts at: 3488 4456 7843 3488
Size: 968 3387 6152
Fragments arranged by size:
6152 3387 968
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2416 2496 2512 2943 3313 2416
Size: 80 16 431 370 9610
Fragments arranged by size:
9610 431 370 80 16
XhoI C'TCGA_G
Cuts at: 1943 4184 1943
Size: 2241 8266
XmaI C'CCGG_G
Cuts at: 1870 2077 2128 7832 1870
Size: 207 51 5704 4545
Fragments arranged by size:
5704 4545 207 51
XmnI GAAnn'nnTTC
Cuts at: 5565 6059 6235 8636 5565
Size: 494 176 2401 7436
Fragments arranged by size:
7436 2401 494 176
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 8315 276
Size: 53 83 186 936 6781 2468
Fragments arranged by size:
6781 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FseI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BfrBI BstZ17I Bsu36I FspAI NruI
NsiI PacI PspXI PsrI RsrII SrfI SwaI