Restriction map for
pFN21AE2743
(Circular) (Six-base) MAPSORT of: pFN21AE2743.seq Check: 9037 from: 1 to: 8122
pFN21AE2743 8122 bp
With 182 enzymes: SgfI *
June 21, 2019 12:02 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3472 3472
Size: 8122
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5932 278
Size: 53 83 186 936 4396 2468
Fragments arranged by size:
4396 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5465 1937
Size: 3528 4594
Acc65I G'GTAC_C
Cuts at: 4800 5443 4800
Size: 643 7479
AclI AA'CG_TT
Cuts at: 1563 6249 6622 1563
Size: 4686 373 3063
Fragments arranged by size:
4686 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3626 3975 4625 4856 5013 6185 7569 7837
Size: 1994 349 650 231 157 1172 1384 268
Cuts at: 7837 1632
Size: 1917
Fragments arranged by size:
1994 1917 1384 1172 650 349 268 231
157
AfeI AGC'GCT
Cuts at: 5079 5079
Size: 8122
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7925
AflIII A'CryG_T
Cuts at: 1203 2190 4963 7021 7998 1203
Size: 987 2773 2058 977 1327
Fragments arranged by size:
2773 2058 1327 987 977
AgeI A'CCGG_T
Cuts at: 6953 6953
Size: 8122
AhdI GACnn_n'nnGTC
Cuts at: 6851 6851
Size: 8122
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8122
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4194 5149 1184
Size: 3010 955 4157
Fragments arranged by size:
4157 3010 955
AlwNI CAG_nnn'CTG
Cuts at: 2302 2320 2515 3424 3637 4018 4149 4834 5865
Size: 18 195 909 213 381 131 685 1031
Cuts at: 5865 7437 2302
Size: 1572 2987
Fragments arranged by size:
2987 1572 1031 909 685 381 213 195
131 18
ApaI G_GGCC'C
Cuts at: 4507 4507
Size: 8122
ApaLI G'TGCA_C
Cuts at: 3233 6179 7335 3233
Size: 2946 1156 4020
Fragments arranged by size:
4020 2946 1156
ApoI r'AATT_y
Cuts at: 1493 2083 2233 4364 4916 5431 5657 5809 7918
Size: 590 150 2131 552 515 226 152 2109
Cuts at: 7918 1493
Size: 1697
Fragments arranged by size:
2131 2109 1697 590 552 515 226 152
150
AseI AT'TA_AT
Cuts at: 160 1032 6676 160
Size: 872 5644 1606
Fragments arranged by size:
5644 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8122
AvaI C'yCGr_G
Cuts at: 1870 1943 2607 3224 4771 5447 1870
Size: 73 664 617 1547 676 4545
Fragments arranged by size:
4545 1547 676 664 617 73
BamHI G'GATC_C
Cuts at: 5008 5452 5819 5008
Size: 444 367 7311
Fragments arranged by size:
7311 444 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4800 5276 5443 6898 618
Size: 325 217 627 3013 476 167 1455 1842
Fragments arranged by size:
3013 1842 1455 627 476 325 217 167
BanII G_rGCy'C
Cuts at: 1391 2462 2695 4507 5359 5441 1391
Size: 1071 233 1812 852 82 4072
Fragments arranged by size:
4072 1812 1071 852 233 82
BbeI G_GCGC'C
Cuts at: 5280 5280
Size: 8122
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2345 2391 3513 4539 928
Size: 971 446 46 1122 1026 4511
Fragments arranged by size:
4511 1122 1026 971 446 46
BbvCI CC'TCA_GC
Cuts at: 2555 3485 4218 4590 4721 2555
Size: 930 733 372 131 5956
Fragments arranged by size:
5956 930 733 372 131
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4013 4047 6313 6347 1903
Size: 34 2076 34 2266 34 3678
Fragments arranged by size:
3678 2266 2076 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3460 3491 4811 4835 6014 7224 3460
Size: 31 1320 24 1179 1210 4358
Fragments arranged by size:
4358 1320 1210 1179 31 24
BclI T'GATC_A
Cuts at: 1548 3063 3933 1548
Size: 1515 870 5737
Fragments arranged by size:
5737 1515 870
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3038 3354 4502
Size: 107 122 71 631 605 1366 316 1148
Cuts at: 4502 6733 136
Size: 2231 1525
Fragments arranged by size:
2231 1525 1366 1148 631 605 316 122
107 71
BglII A'GATC_T
Cuts at: 2656 4908 8117 2656
Size: 2252 3209 2661
Fragments arranged by size:
3209 2661 2252
BlpI GC'TnA_GC
Cuts at: 5539 5539
Size: 8122
Bme1580I G_kGCm'C
Cuts at: 1790 3237 3927 4507 6183 7339 1790
Size: 1447 690 580 1676 1156 2573
Fragments arranged by size:
2573 1676 1447 1156 690 580
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8122
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3436 3844 3910
Size: 416 53 83 399 24 2019 408 66
Cuts at: 3910 5853 6811 442
Size: 1943 958 1753
Fragments arranged by size:
2019 1943 1753 958 416 408 399 83
66 53 24
BmtI G_CTAG'C
Cuts at: 1056 5313 1056
Size: 4257 3865
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3651 3651
Size: 8122
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2312 2345 2516 2546 2564 2951 4529 4666
Size: 1073 33 171 30 18 387 1578 137
Cuts at: 4666 4727 5243 5345 6782 1239
Size: 61 516 102 1437 2579
Fragments arranged by size:
2579 1578 1437 1073 516 387 171 137
102 61 33 30 18
Bpu10I CC'TnA_GC
Cuts at: 2555 2910 3485 4167 4218 4590 4721 4748 2555
Size: 355 575 682 51 372 131 27 5929
Fragments arranged by size:
5929 682 575 372 355 131 51 27
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3027 4175 6247 7112 7410 3027
Size: 1148 2072 865 298 3739
Fragments arranged by size:
3739 2072 1148 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3152 3268 4229 6785 882
Size: 617 1653 116 961 2556 2219
Fragments arranged by size:
2556 2219 1653 961 617 116
BsaAI yAC'GTr
Cuts at: 493 1204 2191 5913 493
Size: 711 987 3722 2702
Fragments arranged by size:
3722 2702 987 711
BsaBI GATnn'nnATC
Cuts at: 2100 8116 2100
Size: 6016 2106
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2346 3099 5277 5929
Size: 53 83 186 936 813 753 2178 652
Cuts at: 5929 6311 6946 275
Size: 382 635 1451
Fragments arranged by size:
2178 1451 936 813 753 652 635 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 5082 5191 6555 6953 7227 7374 7785 5082
Size: 109 1364 398 274 147 411 5419
Fragments arranged by size:
5419 1364 411 398 274 147 109
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4709 4739 1178
Size: 30 165 30 3306 30 4561
Fragments arranged by size:
4561 3306 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2935 3695 1186
Size: 1749 760 5613
Fragments arranged by size:
5613 1749 760
BseYI C'CCAG_C
Cuts at: 2261 2349 2986 3368 3468 3695 3907 4415 4500
Size: 88 637 382 100 227 212 508 85
Cuts at: 4500 4561 5220 7325 7821 2261
Size: 61 659 2105 496 2562
Fragments arranged by size:
2562 2105 659 637 508 496 382 227
212 100 88 85 61
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2288 3091 3861 4028 1163
Size: 573 552 803 770 167 5257
Fragments arranged by size:
5257 803 770 573 552 167
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5572 6333 7361 1540
Size: 275 186 3571 761 1028 2301
Fragments arranged by size:
3571 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2695 3237 3746 5359 6183 6268 7339 2695
Size: 542 509 1613 824 85 1071 3478
Fragments arranged by size:
3478 1613 1071 824 542 509 85
BsmI GAATG_Cn'
Cuts at: 2158 5633 5726 2158
Size: 3475 93 4554
Fragments arranged by size:
4554 3475 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3442 4669 3442
Size: 1227 6895
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2462 2695 3237 3746 3927 4507 5359
Size: 399 672 233 542 509 181 580 852
Cuts at: 5359 5441 6183 6268 7339 1391
Size: 82 742 85 1071 2174
Fragments arranged by size:
2174 1071 852 742 672 580 542 509
399 233 181 85 82
BspEI T'CCGG_A
Cuts at: 5082 5191 7785 5082
Size: 109 2594 5419
Fragments arranged by size:
5419 2594 109
BspHI T'CATG_A
Cuts at: 6011 7741 6011
Size: 1730 6392
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2622 3409 3472 3728 4074 4524 5477 844
Size: 1778 787 63 256 346 450 953 3489
Fragments arranged by size:
3489 1778 953 787 450 346 256 63
BsrBI CCG'CTC
Cuts at: 1394 1823 2599 6009 1394
Size: 429 776 3410 3507
Fragments arranged by size:
3507 3410 776 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3129 4029 4677 5052 6617 6791 65
Size: 1183 1881 900 648 375 1565 174 1396
Fragments arranged by size:
1881 1565 1396 1183 900 648 375 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4081 4584 5226 5315 6766 6953 1700
Size: 110 2271 503 642 89 1451 187 2869
Fragments arranged by size:
2869 2271 1451 642 503 187 110 89
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8122
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8122
BssSI C'ACGA_G
Cuts at: 6182 7194 6182
Size: 1012 7110
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2098 2302 794
Size: 1304 204 6614
Fragments arranged by size:
6614 1304 204
BstBI TT'CG_AA
Cuts at: 6926 6926
Size: 8122
BstEII G'GTnAC_C
Cuts at: 1184 5209 1184
Size: 4025 4097
BstXI CCAn_nnnn'nTGG
Cuts at: 2884 3310 4644 2884
Size: 426 1334 6362
Fragments arranged by size:
6362 1334 426
BstYI r'GATC_y
Cuts at: 1972 2164 2538 2656 4104 4314 4410 4908 5008
Size: 192 374 118 1448 210 96 498 100
Cuts at: 5008 5452 5819 6204 6221 7673 7759 7771 8117
Size: 444 367 385 17 1452 86 12 346
Cuts at: 8117 1972
Size: 1977
Fragments arranged by size:
1977 1452 1448 498 444 385 374 367
346 210 192 118 100 96 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2333 2566 3080 3678 3998 2333
Size: 233 514 598 320 6457
Fragments arranged by size:
6457 598 514 320 233
BtgI C'CryG_G
Cuts at: 513 1065 2056 2277 3135 4441 4551 5166 5202
Size: 552 991 221 858 1306 110 615 36
Cuts at: 5202 513
Size: 3433
Fragments arranged by size:
3433 1306 991 858 615 552 221 110
36
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7383
Fragments arranged by size:
7383 683 56
BtsI GCAGTG_nn'
Cuts at: 2156 2361 2652 3359 3510 4019 4188 4408 5240
Size: 205 291 707 151 509 169 220 832
Cuts at: 5240 5638 6432 6452 2156
Size: 398 794 20 3826
Fragments arranged by size:
3826 832 794 707 509 398 291 220
205 169 151 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8122
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8087
DraI TTT'AAA
Cuts at: 5426 5426
Size: 8122
DraIII CAC_nnn'GTG
Cuts at: 5019 5019
Size: 8122
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7129 809
Size: 507 5813 1802
Fragments arranged by size:
5813 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4341 4482 4932
Size: 54 1425 50 275 18 2511 141 450
Cuts at: 4932 5406 5569 6458 8087 8
Size: 474 163 889 1629 43
Fragments arranged by size:
2511 1629 1425 889 474 450 275 163
141 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5569 1537
Size: 275 3757 4090
Fragments arranged by size:
4090 3757 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3941 4465 4832 6052 1353
Size: 2588 524 367 1220 3423
Fragments arranged by size:
3423 2588 1220 524 367
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2138 6709 7083 7229 387
Size: 1064 138 549 4571 374 146 1280
Fragments arranged by size:
4571 1280 1064 549 374 146 138
EcoICRI GAG'CTC
Cuts at: 2693 5357 2693
Size: 2664 5458
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2312 2345 2516 2546 2564 2951 3626
Size: 393 680 33 171 30 18 387 675
Cuts at: 3626 3975 4529 4625 4666 4727 4856 5013 5243
Size: 349 554 96 41 61 129 157 230
Cuts at: 5243 5345 6185 6782 7569 7837 1239
Size: 102 840 597 787 268 1524
Fragments arranged by size:
1524 840 787 680 675 597 554 393
387 349 268 230 171 157 129 102
96 61 41 33 30 18
EcoNI CCTnn'n_nnAGG
Cuts at: 2546 2618 3257 5261 2546
Size: 72 639 2004 5407
Fragments arranged by size:
5407 2004 639 72
EcoO109I rG'GnC_Cy
Cuts at: 2329 2562 2611 3421 4253 4347 4504 4513 4831
Size: 233 49 810 832 94 157 9 318
Cuts at: 4831 4944 2329
Size: 113 5507
Fragments arranged by size:
5507 832 810 318 233 157 113 94
49 9
EcoRI G'AATT_C
Cuts at: 2233 4364 4916 5431 5809 2233
Size: 2131 552 515 378 4546
Fragments arranged by size:
4546 2131 552 515 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2416 3068 2416
Size: 652 7470
FspI TGC'GCA
Cuts at: 6628 6628
Size: 8122
HaeII r_GCGC'y
Cuts at: 1678 5081 5227 5270 5280 5316 7269 1678
Size: 3403 146 43 10 36 1953 2531
Fragments arranged by size:
3403 2531 1953 146 43 36 10
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2192 2224 2657 2689 4622
Size: 32 135 32 620 32 433 32 1933
Cuts at: 4622 4654 4709 4741 4809 4841 6763 6795 6837
Size: 32 55 32 68 32 1922 32 42
Cuts at: 6837 6869 1373
Size: 32 2626
Fragments arranged by size:
2626 1933 1922 620 433 135 68 55
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5466 5712 669
Size: 988 281 3528 246 3079
Fragments arranged by size:
3528 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8122
HpaI GTT'AAC
Cuts at: 5712 5712
Size: 8122
KasI G'GCGC_C
Cuts at: 5276 5276
Size: 8122
KpnI G_GTAC'C
Cuts at: 4804 5447 4804
Size: 643 7479
MfeI C'AATT_G
Cuts at: 5721 5721
Size: 8122
MluI A'CGCG_T
Cuts at: 7998 7998
Size: 8122
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2973 3156 3477 3936 7236 7420 1305
Size: 1668 183 321 459 3300 184 2007
Fragments arranged by size:
3300 2007 1668 459 321 184 183
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4343 4934 5408 10
Size: 54 1425 343 2511 591 474 2724
Fragments arranged by size:
2724 2511 1425 591 474 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2366 3134 3732 6080 6439 6598 518
Size: 648 1200 768 598 2348 359 159 2042
Fragments arranged by size:
2348 2042 1200 768 648 598 359 159
MspA1I CmG'CkG
Cuts at: 1606 2884 3703 4594 4714 5060 5168 5204 5248
Size: 1278 819 891 120 346 108 36 44
Cuts at: 5248 5538 6215 7363 7608 1606
Size: 290 677 1148 245 2120
Fragments arranged by size:
2120 1278 1148 891 819 677 346 290
245 120 108 44 36
NaeI GCC'GGC
Cuts at: 4083 4586 5228 4083
Size: 503 642 6977
Fragments arranged by size:
6977 642 503
NarI GG'CG_CC
Cuts at: 5277 5277
Size: 8122
NcoI C'CATG_G
Cuts at: 513 1065 2056 3135 4441 513
Size: 552 991 1079 1306 4194
Fragments arranged by size:
4194 1306 1079 991 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8122
NgoMIV G'CCGG_C
Cuts at: 4081 4584 5226 4081
Size: 503 642 6977
Fragments arranged by size:
6977 642 503
NheI G'CTAG_C
Cuts at: 1052 5309 1052
Size: 4257 3865
NotI GC'GGCC_GC
Cuts at: 5569 5569
Size: 8122
NspI r_CATG'y
Cuts at: 1132 1229 4636 4967 5480 7025 1132
Size: 97 3407 331 513 1545 2229
Fragments arranged by size:
3407 2229 1545 513 331 97
PasI CC'CwG_GG
Cuts at: 1348 3163 4113 4669 5185 1348
Size: 1815 950 556 516 4285
Fragments arranged by size:
4285 1815 950 556 516
PciI A'CATG_T
Cuts at: 4963 7021 4963
Size: 2058 6064
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2986 4119 5019 1108
Size: 1878 1133 900 4211
Fragments arranged by size:
4211 1878 1133 900
PfoI T'CCnGG_A
Cuts at: 2237 2702 3123 3768 4827 2237
Size: 465 421 645 1059 5532
Fragments arranged by size:
5532 1059 645 465 421
PmeI GTTT'AAAC
Cuts at: 5426 5426
Size: 8122
PmlI CAC'GTG
Cuts at: 2191 2191
Size: 8122
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6191 6223 7704 7736 6191
Size: 32 1481 32 6577
Fragments arranged by size:
6577 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2329 2562 2611 3421 4253 4347 4513 4831 4944
Size: 233 49 810 832 94 166 318 113
Cuts at: 4944 2329
Size: 5507
Fragments arranged by size:
5507 832 810 318 233 166 113 94
49
PshAI GACnn'nnGTC
Cuts at: 1613 3992 1613
Size: 2379 5743
PsiI TTA'TAA
Cuts at: 4904 5692 4904
Size: 788 7334
PspOMI G'GGCC_C
Cuts at: 4503 4503
Size: 8122
PstI C_TGCA'G
Cuts at: 830 2271 2647 2724 3395 4183 4958 5247 5253
Size: 1441 376 77 671 788 775 289 6
Cuts at: 5253 5474 830
Size: 221 3478
Fragments arranged by size:
3478 1441 788 775 671 376 289 221
77 6
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8122
PvuII CAG'CTG
Cuts at: 2884 3703 4594 4714 5060 5248 2884
Size: 819 891 120 346 188 5758
Fragments arranged by size:
5758 891 819 346 188 120
SacI G_AGCT'C
Cuts at: 2695 5359 2695
Size: 2664 5458
SacII CC_GC'GG
Cuts at: 5169 5205 5169
Size: 36 8086
SalI G'TCGA_C
Cuts at: 1936 5464 1936
Size: 3528 4594
SanDI GG'GwC_CC
Cuts at: 4253 4944 4253
Size: 691 7431
SapI GCTCTTCn'nnn_
Cuts at: 3941 4832 3941
Size: 891 7231
SbfI CC_TGCA'GG
Cuts at: 2271 5474 2271
Size: 3203 4919
ScaI AGT'ACT
Cuts at: 4266 6370 8082 4266
Size: 2104 1712 4306
Fragments arranged by size:
4306 2104 1712
SexAI A'CCwGG_T
Cuts at: 3258 3258
Size: 8122
SfcI C'TryA_G
Cuts at: 826 1045 2267 2643 2720 2838 3199 3391 3597
Size: 219 1222 376 77 118 361 192 206
Cuts at: 3597 4179 4293 4954 5243 5249 5470 6605 7286
Size: 582 114 661 289 6 221 1135 681
Cuts at: 7286 7477 826
Size: 191 1471
Fragments arranged by size:
1471 1222 1135 681 661 582 376 361
289 221 219 206 192 191 118 114
77 6
SfoI GGC'GCC
Cuts at: 5278 5278
Size: 8122
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8122
SgrAI Cr'CCGG_yG
Cuts at: 1700 5226 1700
Size: 3526 4596
SmaI CCC'GGG
Cuts at: 1872 4773 5449 1872
Size: 2901 676 4545
Fragments arranged by size:
4545 2901 676
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3042 4190 6226 7127 7389 820
Size: 197 926 1099 1148 2036 901 262 1553
Fragments arranged by size:
2036 1553 1148 1099 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5913 493
Size: 5420 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8122
SphI G_CATG'C
Cuts at: 1132 5480 1132
Size: 4348 3774
SspI AAT'ATT
Cuts at: 5 52 6046 5
Size: 47 5994 2081
Fragments arranged by size:
5994 2081 47
StuI AGG'CCT
Cuts at: 1517 3386 3676 1517
Size: 1869 290 5963
Fragments arranged by size:
5963 1869 290
StyI C'CwwG_G
Cuts at: 513 1065 2056 3135 3963 4441 5561 513
Size: 552 991 1079 828 478 1120 3074
Fragments arranged by size:
3074 1120 1079 991 828 552 478
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3395 6149 6166 6319 6504 6949 325
Size: 1255 1815 2754 17 153 185 445 1498
Fragments arranged by size:
2754 1815 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4041 4264 6368
Size: 274 80 33 51 1443 2064 223 2104
Cuts at: 6368 8080 96
Size: 1712 138
Fragments arranged by size:
2104 2064 1712 1443 274 223 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4291 4323 4387 4419 4505 4537 8082 8114 4291
Size: 32 64 32 86 32 3545 32 4299
Fragments arranged by size:
4299 3545 86 64 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2891 1316
Size: 1575 6547
XbaI T'CTAG_A
Cuts at: 5458 5458
Size: 8122
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2881 2983 4246 4735 2881
Size: 102 1263 489 6268
Fragments arranged by size:
6268 1263 489 102
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8122
XmaI C'CCGG_G
Cuts at: 1870 4771 5447 1870
Size: 2901 676 4545
Fragments arranged by size:
4545 2901 676
XmnI GAAnn'nnTTC
Cuts at: 3785 6251 3785
Size: 2466 5656
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5930 276
Size: 53 83 186 936 4396 2468
Fragments arranged by size:
4396 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I EcoRV
FseI FspAI NruI NsiI PacI PspXI PsrI RsrII
SfiI SrfI SwaI