Restriction map for
pFN21AE2737
(Circular) (Six-base) MAPSORT of: pFN21AE2737.seq Check: 3035 from: 1 to: 5548
pFN21AE2737 5548 bp
With 182 enzymes: SgfI *
December 20, 2018 17:18 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2370 3358 278
Size: 53 83 186 936 834 988 2468
Fragments arranged by size:
2468 988 936 834 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2823 2891 1937
Size: 886 68 4594
Fragments arranged by size:
4594 886 68
Acc65I G'GTAC_C
Cuts at: 2869 2869
Size: 5548
AclI AA'CG_TT
Cuts at: 1563 3675 4048 1563
Size: 2112 373 3063
Fragments arranged by size:
3063 2112 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3611 4995 5263 1632
Size: 1979 1384 268 1917
Fragments arranged by size:
1979 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5351
AflIII A'CryG_T
Cuts at: 1203 4447 5424 1203
Size: 3244 977 1327
Fragments arranged by size:
3244 1327 977
AgeI A'CCGG_T
Cuts at: 4379 4379
Size: 5548
AhdI GACnn_n'nnGTC
Cuts at: 4277 4277
Size: 5548
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5548
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2668 1184
Size: 1484 4064
AlwNI CAG_nnn'CTG
Cuts at: 3291 4863 3291
Size: 1572 3976
ApaI G_GGCC'C
Cuts at: 2242 2344 2242
Size: 102 5446
ApaLI G'TGCA_C
Cuts at: 3605 4761 3605
Size: 1156 4392
ApoI r'AATT_y
Cuts at: 1493 2857 3083 3235 5344 1493
Size: 1364 226 152 2109 1697
Fragments arranged by size:
2109 1697 1364 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4102 160
Size: 872 3070 1606
Fragments arranged by size:
3070 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5548
AvaI C'yCGr_G
Cuts at: 1870 1943 2405 2768 2774 2873 1870
Size: 73 462 363 6 99 4545
Fragments arranged by size:
4545 462 363 99 73 6
BamHI G'GATC_C
Cuts at: 2109 2878 3245 2109
Size: 769 367 4412
Fragments arranged by size:
4412 769 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2396 2869 4324 618
Size: 325 217 627 609 473 1455 1842
Fragments arranged by size:
1842 1455 627 609 473 325 217
BanII G_rGCy'C
Cuts at: 1391 2242 2253 2344 2775 2867 1391
Size: 851 11 91 431 92 4072
Fragments arranged by size:
4072 851 431 92 91 11
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4577
BbvCI CC'TCA_GC
Cuts at: 2027 2027
Size: 5548
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3739 3773 1903
Size: 34 1802 34 3678
Fragments arranged by size:
3678 1802 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3440 4650 3440
Size: 1210 4338
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5548
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2265 4159 136
Size: 107 122 71 631 605 593 1894 1525
Fragments arranged by size:
1894 1525 631 605 593 122 107 71
BglII A'GATC_T
Cuts at: 2169 5543 2169
Size: 3374 2174
BlpI GC'TnA_GC
Cuts at: 2606 2965 2606
Size: 359 5189
Bme1580I G_kGCm'C
Cuts at: 1790 2242 2344 2538 3609 4765 1790
Size: 452 102 194 1071 1156 2573
Fragments arranged by size:
2573 1156 1071 452 194 102
BmgBI CAC'GTC
Cuts at: 1318 2821 1318
Size: 1503 4045
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3279 4237 442
Size: 416 53 83 399 24 1862 958 1753
Fragments arranged by size:
1862 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5548
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2369 2690 4208 1239
Size: 1130 321 1518 2579
Fragments arranged by size:
2579 1518 1130 321
Bpu10I CC'TnA_GC
Cuts at: 2027 2027
Size: 5548
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2228 3673 4538 4836 2228
Size: 1445 865 298 2940
Fragments arranged by size:
2940 1445 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2405 4211 882
Size: 617 906 1806 2219
Fragments arranged by size:
2219 1806 906 617
BsaAI yAC'GTr
Cuts at: 493 1204 3339 493
Size: 711 2135 2702
Fragments arranged by size:
2702 2135 711
BsaBI GATnn'nnATC
Cuts at: 5542 5542
Size: 5548
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2367 2757 3355 3737
Size: 53 83 186 936 834 390 598 382
Cuts at: 3737 4372 275
Size: 635 1451
Fragments arranged by size:
1451 936 834 635 598 390 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3981 4379 4653 4800 5211 3981
Size: 398 274 147 411 4318
Fragments arranged by size:
4318 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5323
Fragments arranged by size:
5323 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2423 2438 1186
Size: 1237 15 4296
Fragments arranged by size:
4296 1237 15
BseYI C'CCAG_C
Cuts at: 2343 2430 2601 4751 5247 2343
Size: 87 171 2150 496 2644
Fragments arranged by size:
2644 2150 496 171 87
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2462 2664 2702 2824 1163
Size: 573 726 202 38 122 3887
Fragments arranged by size:
3887 726 573 202 122 38
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2421 2998 3759 4787 1540
Size: 275 186 420 577 761 1028 2301
Fragments arranged by size:
2301 1028 761 577 420 275 186
BsiHKAI G_wGCw'C
Cuts at: 2253 2775 3609 3694 4765 2253
Size: 522 834 85 1071 3036
Fragments arranged by size:
3036 1071 834 522 85
BsmI GAATG_Cn'
Cuts at: 3059 3152 3059
Size: 93 5455
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2242 2253 2344 2538 2775 2867 3609
Size: 399 452 11 91 194 237 92 742
Cuts at: 3609 3694 4765 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 452 399 237 194 92
91 85 11
BspEI T'CCGG_A
Cuts at: 5211 5211
Size: 5548
BspHI T'CATG_A
Cuts at: 2781 3437 5167 2781
Size: 656 1730 3162
Fragments arranged by size:
3162 1730 656
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2903 844
Size: 2059 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3435 1394
Size: 429 1612 3507
Fragments arranged by size:
3507 1612 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4043 4217 65
Size: 1183 2795 174 1396
Fragments arranged by size:
2795 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2829 4192 4379 1700
Size: 110 1019 1363 187 2869
Fragments arranged by size:
2869 1363 1019 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5548
BssHII G'CGCG_C
Cuts at: 1925 2694 1925
Size: 769 4779
BssSI C'ACGA_G
Cuts at: 3608 4620 3608
Size: 1012 4536
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2533 794
Size: 1739 3809
BstBI TT'CG_AA
Cuts at: 4352 4352
Size: 5548
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5548
BstXI CCAn_nnnn'nTGG
Cuts at: 2185 2185
Size: 5548
BstYI r'GATC_y
Cuts at: 1972 2109 2169 2878 3245 3630 3647 5099 5185
Size: 137 60 709 367 385 17 1452 86
Cuts at: 5185 5197 5543 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 709 385 367 346 137 86
60 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2208 2415 513
Size: 552 1143 207 3646
Fragments arranged by size:
3646 1143 552 207
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2200 487
Size: 683 56 974 3835
Fragments arranged by size:
3835 974 683 56
BtsI GCAGTG_nn'
Cuts at: 3064 3858 3878 3064
Size: 794 20 4734
Fragments arranged by size:
4734 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5548
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5513
DraI TTT'AAA
Cuts at: 2852 2852
Size: 5548
DraIII CAC_nnn'GTG
Cuts at: 2533 2533
Size: 5548
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4555 809
Size: 507 3239 1802
Fragments arranged by size:
3239 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2187 2418 2995
Size: 54 1425 50 275 18 357 231 577
Cuts at: 2995 3884 5513 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 577 357 275 231 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2418 2995 1537
Size: 275 606 577 4090
Fragments arranged by size:
4090 606 577 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2054 2069 3478 1353
Size: 701 15 1409 3423
Fragments arranged by size:
3423 1409 701 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4135 4509 4655 387
Size: 1064 138 2546 374 146 1280
Fragments arranged by size:
2546 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2251 2773 2251
Size: 522 5026
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2369 2690 3611 4208 4995 5263 1239
Size: 393 737 321 921 597 787 268 1524
Fragments arranged by size:
1524 921 787 737 597 393 321 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2585 2585
Size: 5548
EcoO109I rG'GnC_Cy
Cuts at: 2238 2239 2701 2238
Size: 1 462 5085
Fragments arranged by size:
5085 462 1
EcoRI G'AATT_C
Cuts at: 2857 3235 2857
Size: 378 5170
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2146 2611 2146
Size: 465 5083
FspI TGC'GCA
Cuts at: 4054 4054
Size: 5548
HaeII r_GCGC'y
Cuts at: 1678 4695 1678
Size: 3017 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4189 4221 4263 4295 1373
Size: 32 135 32 2617 32 42 32 2626
Fragments arranged by size:
2626 2617 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2892 3138 669
Size: 988 281 954 246 3079
Fragments arranged by size:
3079 988 954 281 246
HindIII A'AGCT_T
Cuts at: 748 2356 748
Size: 1608 3940
HpaI GTT'AAC
Cuts at: 3138 3138
Size: 5548
KpnI G_GTAC'C
Cuts at: 2873 2873
Size: 5548
MfeI C'AATT_G
Cuts at: 3147 3147
Size: 5548
MluI A'CGCG_T
Cuts at: 5424 5424
Size: 5548
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2499 4662 4846 1305
Size: 1194 2163 184 2007
Fragments arranged by size:
2163 2007 1194 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2189 10
Size: 54 1425 343 357 3369
Fragments arranged by size:
3369 1425 357 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3506 3865 4024 518
Size: 648 2340 359 159 2042
Fragments arranged by size:
2340 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2256 2338 2347 2417 2452 2964 3641 4789
Size: 650 82 9 70 35 512 677 1148
Cuts at: 4789 5034 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 650 512 245 82 70
35 9
NcoI C'CATG_G
Cuts at: 513 1065 2208 513
Size: 552 1143 3853
Fragments arranged by size:
3853 1143 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5548
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5548
NotI GC'GGCC_GC
Cuts at: 2995 2995
Size: 5548
NspI r_CATG'y
Cuts at: 1132 1229 2906 4451 1132
Size: 97 1677 1545 2229
Fragments arranged by size:
2229 1677 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2314 1348
Size: 966 4582
PciI A'CATG_T
Cuts at: 4447 4447
Size: 5548
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5548
PmeI GTTT'AAAC
Cuts at: 2852 2852
Size: 5548
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2762 2794 3617 3649 5130 5162 2762
Size: 32 823 32 1481 32 3148
Fragments arranged by size:
3148 1481 823 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5548
PsiI TTA'TAA
Cuts at: 3118 3118
Size: 5548
PspOMI G'GGCC_C
Cuts at: 2238 2340 2238
Size: 102 5446
PspXI vC'TCGA_Gb
Cuts at: 2768 2768
Size: 5548
PstI C_TGCA'G
Cuts at: 830 2169 2668 2900 830
Size: 1339 499 232 3478
Fragments arranged by size:
3478 1339 499 232
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5548
PvuII CAG'CTG
Cuts at: 2347 2452 2347
Size: 105 5443
SacI G_AGCT'C
Cuts at: 2253 2775 2253
Size: 522 5026
SacII CC_GC'GG
Cuts at: 2418 2418
Size: 5548
SalI G'TCGA_C
Cuts at: 1936 2890 1936
Size: 954 4594
SbfI CC_TGCA'GG
Cuts at: 2900 2900
Size: 5548
ScaI AGT'ACT
Cuts at: 2042 3796 5508 2042
Size: 1754 1712 2082
Fragments arranged by size:
2082 1754 1712
SfcI C'TryA_G
Cuts at: 826 1045 2165 2664 2896 4031 4712 4903 826
Size: 219 1120 499 232 1135 681 191 1471
Fragments arranged by size:
1471 1135 1120 681 499 232 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5548
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5548
SmaI CCC'GGG
Cuts at: 1872 2875 1872
Size: 1003 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2243 2768 3652 4553 4815 820
Size: 197 926 300 525 884 901 262 1553
Fragments arranged by size:
1553 926 901 884 525 300 262 197
SnaBI TAC'GTA
Cuts at: 493 3339 493
Size: 2846 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5548
SphI G_CATG'C
Cuts at: 1132 2906 1132
Size: 1774 3774
SspI AAT'ATT
Cuts at: 5 52 3472 5
Size: 47 3420 2081
Fragments arranged by size:
3420 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5548
StyI C'CwwG_G
Cuts at: 513 1065 2208 2987 513
Size: 552 1143 779 3074
Fragments arranged by size:
3074 1143 779 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3575 3592 3745 3930 4375 325
Size: 1255 1995 17 153 185 445 1498
Fragments arranged by size:
1995 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2040 3794 5506
Size: 274 80 33 51 1443 63 1754 1712
Cuts at: 5506 96
Size: 138
Fragments arranged by size:
1754 1712 1443 274 138 80 63 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5508 5540 5508
Size: 32 5516
Tth111I GACn'n_nGTC
Cuts at: 1316 2819 1316
Size: 1503 4045
XbaI T'CTAG_A
Cuts at: 2884 2884
Size: 5548
XhoI C'TCGA_G
Cuts at: 1943 2768 1943
Size: 825 4723
XmaI C'CCGG_G
Cuts at: 1870 2873 1870
Size: 1003 4545
XmnI GAAnn'nnTTC
Cuts at: 3677 3677
Size: 5548
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2368 3356 276
Size: 53 83 186 936 834 988 2468
Fragments arranged by size:
2468 988 936 834 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI
SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PpuMI PsrI RsrII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI