Restriction map for
pFN21AE2733
(Circular) (Six-base) MAPSORT of: pFN21AE2733.seq Check: 5385 from: 1 to: 5749
pFN21AE2733 5749 bp
With 182 enzymes: SgfI *
September 11, 2018 19:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2610 2610
Size: 5749
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3559 278
Size: 53 83 186 936 2023 2468
Fragments arranged by size:
2468 2023 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2592 3092 1937
Size: 655 500 4594
Fragments arranged by size:
4594 655 500
Acc65I G'GTAC_C
Cuts at: 2189 3070 2189
Size: 881 4868
AclI AA'CG_TT
Cuts at: 1563 3876 4249 1563
Size: 2313 373 3063
Fragments arranged by size:
3063 2313 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3812 5196 5464 1632
Size: 2180 1384 268 1917
Fragments arranged by size:
2180 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5552
AflIII A'CryG_T
Cuts at: 1203 4648 5625 1203
Size: 3445 977 1327
Fragments arranged by size:
3445 1327 977
AgeI A'CCGG_T
Cuts at: 4580 4580
Size: 5749
AhdI GACnn_n'nnGTC
Cuts at: 2962 4478 2962
Size: 1516 4233
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5749
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2064 1184
Size: 880 4869
AlwNI CAG_nnn'CTG
Cuts at: 2150 2176 2695 2835 2861 3492 5064 2150
Size: 26 519 140 26 631 1572 2835
Fragments arranged by size:
2835 1572 631 519 140 26 26
ApaI G_GGCC'C
Cuts at: 2032 2032
Size: 5749
ApaLI G'TGCA_C
Cuts at: 3806 4962 3806
Size: 1156 4593
ApoI r'AATT_y
Cuts at: 1493 2139 3058 3284 3436 5545 1493
Size: 646 919 226 152 2109 1697
Fragments arranged by size:
2109 1697 919 646 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4303 160
Size: 872 3271 1606
Fragments arranged by size:
3271 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5749
AvaI C'yCGr_G
Cuts at: 1870 1943 2086 2358 2463 2515 2810 3074 1870
Size: 73 143 272 105 52 295 264 4545
Fragments arranged by size:
4545 295 272 264 143 105 73 52
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2724 2757 2724
Size: 33 5716
BamHI G'GATC_C
Cuts at: 3079 3446 3079
Size: 367 5382
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2189 2798 3070 4525 618
Size: 325 217 627 402 609 272 1455 1842
Fragments arranged by size:
1842 1455 627 609 402 325 272 217
BanII G_rGCy'C
Cuts at: 1391 2032 2337 2478 2940 3068 1391
Size: 641 305 141 462 128 4072
Fragments arranged by size:
4072 641 462 305 141 128
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4778
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3940 3974 1903
Size: 34 2003 34 3678
Fragments arranged by size:
3678 2003 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3641 4851 3641
Size: 1210 4539
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5749
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2897 3029 4360
Size: 107 122 71 631 605 1225 132 1331
Cuts at: 4360 136
Size: 1525
Fragments arranged by size:
1525 1331 1225 631 605 132 122 107
71
BglII A'GATC_T
Cuts at: 5744 5744
Size: 5749
BlpI GC'TnA_GC
Cuts at: 3166 3166
Size: 5749
Bme1580I G_kGCm'C
Cuts at: 1790 2032 3810 4966 1790
Size: 242 1778 1156 2573
Fragments arranged by size:
2573 1778 1156 242
BmgBI CAC'GTC
Cuts at: 1318 2740 2848 1318
Size: 1422 108 4219
Fragments arranged by size:
4219 1422 108
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2186 2255 2400
Size: 416 53 83 399 24 769 69 145
Cuts at: 2400 3480 4438 442
Size: 1080 958 1753
Fragments arranged by size:
1753 1080 958 769 416 399 145 83
69 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5749
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2410 2437 2981 4409 1239
Size: 1171 27 544 1428 2579
Fragments arranged by size:
2579 1428 1171 544 27
Bpu10I CC'TnA_GC
Cuts at: 2522 2839 2522
Size: 317 5432
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3874 4739 5037 3874
Size: 865 298 4586
Fragments arranged by size:
4586 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2098 2495 4412 882
Size: 617 599 397 1917 2219
Fragments arranged by size:
2219 1917 617 599 397
BsaAI yAC'GTr
Cuts at: 493 1204 3540 493
Size: 711 2336 2702
Fragments arranged by size:
2702 2336 711
BsaBI GATnn'nnATC
Cuts at: 5743 5743
Size: 5749
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2274 3556 3938 4573
Size: 53 83 186 936 741 1282 382 635
Cuts at: 4573 275
Size: 1451
Fragments arranged by size:
1451 1282 936 741 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4182 4580 4854 5001 5412 4182
Size: 398 274 147 411 4519
Fragments arranged by size:
4519 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2173 2203 2526 2556 1178
Size: 30 165 30 770 30 323 30 4371
Fragments arranged by size:
4371 770 323 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2486 2552 2635 1186
Size: 1300 66 83 4300
Fragments arranged by size:
4300 1300 83 66
BseYI C'CCAG_C
Cuts at: 2205 2255 2791 2854 2882 4952 5448 2205
Size: 50 536 63 28 2070 496 2506
Fragments arranged by size:
2506 2070 536 496 63 50 28
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2234 2477 2639 2930 1163
Size: 573 498 243 162 291 3982
Fragments arranged by size:
3982 573 498 291 243 162
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3199 3960 4988 1540
Size: 275 186 1198 761 1028 2301
Fragments arranged by size:
2301 1198 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2337 2426 2982 3810 3895 4966 2337
Size: 89 556 828 85 1071 3120
Fragments arranged by size:
3120 1071 828 556 89 85
BsmI GAATG_Cn'
Cuts at: 3260 3353 3260
Size: 93 5656
BsmBI CGTCTCn'nnnn_
Cuts at: 2281 2838 2281
Size: 557 5192
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2032 2337 2426 2478 2940 2982 3068
Size: 399 242 305 89 52 462 42 86
Cuts at: 3068 3810 3895 4966 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 462 399 305 242 89
86 85 52 42
BspEI T'CCGG_A
Cuts at: 5412 5412
Size: 5749
BspHI T'CATG_A
Cuts at: 3638 5368 3638
Size: 1730 4019
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2610 3104 844
Size: 1766 494 3489
Fragments arranged by size:
3489 1766 494
BsrBI CCG'CTC
Cuts at: 1394 1823 2392 2509 2942 3636 1394
Size: 429 569 117 433 694 3507
Fragments arranged by size:
3507 694 569 433 429 117
BsrDI GCAATG_nn'
Cuts at: 65 1248 4244 4418 65
Size: 1183 2996 174 1396
Fragments arranged by size:
2996 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2570 2605 2795 4393 4580 1700
Size: 110 760 35 190 1598 187 2869
Fragments arranged by size:
2869 1598 760 190 187 110 35
BsrGI T'GTAC_A
Cuts at: 96 2016 96
Size: 1920 3829
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5749
BssSI C'ACGA_G
Cuts at: 2933 3809 4821 2933
Size: 876 1012 3861
Fragments arranged by size:
3861 1012 876
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2835 794
Size: 2041 3708
BstBI TT'CG_AA
Cuts at: 4553 4553
Size: 5749
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5749
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2115 2011
Size: 104 5645
BstYI r'GATC_y
Cuts at: 1972 2400 2831 3079 3446 3831 3848 5300 5386
Size: 428 431 248 367 385 17 1452 86
Cuts at: 5386 5398 5744 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 431 428 385 367 346 248
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2679 2679
Size: 5749
BtgI C'CryG_G
Cuts at: 513 1065 2496 513
Size: 552 1431 3766
Fragments arranged by size:
3766 1431 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5010
Fragments arranged by size:
5010 683 56
BtsI GCAGTG_nn'
Cuts at: 2755 2922 3008 3265 4059 4079 2755
Size: 167 86 257 794 20 4425
Fragments arranged by size:
4425 794 257 167 86 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5749
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5714
DraI TTT'AAA
Cuts at: 3053 3053
Size: 5749
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4756 809
Size: 507 3440 1802
Fragments arranged by size:
3440 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3196 4085 5714
Size: 54 1425 50 275 18 1366 889 1629
Cuts at: 5714 8
Size: 43
Fragments arranged by size:
1629 1425 1366 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3196 1537
Size: 275 1384 4090
Fragments arranged by size:
4090 1384 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3679 1353
Size: 2326 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2601 4336 4710 4856 387
Size: 1064 138 1012 1735 374 146 1280
Fragments arranged by size:
1735 1280 1064 1012 374 146 138
EcoICRI GAG'CTC
Cuts at: 2335 2335
Size: 5749
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2410 2437 2981 3812 4409 5196 5464
Size: 393 778 27 544 831 597 787 268
Cuts at: 5464 1239
Size: 1524
Fragments arranged by size:
1524 831 787 778 597 544 393 268
27
EcoO109I rG'GnC_Cy
Cuts at: 2028 2029 2147 2412 2675 2028
Size: 1 118 265 263 5102
Fragments arranged by size:
5102 265 263 118 1
EcoRI G'AATT_C
Cuts at: 3058 3436 3058
Size: 378 5371
FspI TGC'GCA
Cuts at: 4255 4255
Size: 5749
HaeII r_GCGC'y
Cuts at: 1678 2782 4896 1678
Size: 1104 2114 2531
Fragments arranged by size:
2531 2114 1104
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2948 2980 4390 4422 4464
Size: 32 135 32 1376 32 1410 32 42
Cuts at: 4464 4496 1373
Size: 32 2626
Fragments arranged by size:
2626 1410 1376 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3093 3339 669
Size: 988 281 1155 246 3079
Fragments arranged by size:
3079 1155 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5749
HpaI GTT'AAC
Cuts at: 3339 3339
Size: 5749
KpnI G_GTAC'C
Cuts at: 2193 3074 2193
Size: 881 4868
MfeI C'AATT_G
Cuts at: 3348 3348
Size: 5749
MluI A'CGCG_T
Cuts at: 5625 5625
Size: 5749
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2081 4863 5047 1305
Size: 776 2782 184 2007
Fragments arranged by size:
2782 2007 776 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3927
Fragments arranged by size:
3927 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2952 3707 4066 4225 518
Size: 648 1786 755 359 159 2042
Fragments arranged by size:
2042 1786 755 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2430 2629 2765 3165 3842 4990 5235 1606
Size: 824 199 136 400 677 1148 245 2120
Fragments arranged by size:
2120 1148 824 677 400 245 199 136
NaeI GCC'GGC
Cuts at: 2572 2607 2797 2572
Size: 35 190 5524
Fragments arranged by size:
5524 190 35
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5197
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5749
NgoMIV G'CCGG_C
Cuts at: 2570 2605 2795 2570
Size: 35 190 5524
Fragments arranged by size:
5524 190 35
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5749
NotI GC'GGCC_GC
Cuts at: 3196 3196
Size: 5749
NspI r_CATG'y
Cuts at: 1132 1229 3107 4652 1132
Size: 97 1878 1545 2229
Fragments arranged by size:
2229 1878 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2903 1348
Size: 1555 4194
PciI A'CATG_T
Cuts at: 4648 4648
Size: 5749
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3019 1108
Size: 1911 3838
PfoI T'CCnGG_A
Cuts at: 2394 2448 2394
Size: 54 5695
PmeI GTTT'AAAC
Cuts at: 3053 3053
Size: 5749
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3818 3850 5331 5363 3818
Size: 32 1481 32 4204
Fragments arranged by size:
4204 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2412 2675 2412
Size: 263 5486
PshAI GACnn'nnGTC
Cuts at: 1613 2588 1613
Size: 975 4774
PsiI TTA'TAA
Cuts at: 3319 3319
Size: 5749
PspOMI G'GGCC_C
Cuts at: 2028 2028
Size: 5749
PstI C_TGCA'G
Cuts at: 830 2439 2623 2831 3003 3101 830
Size: 1609 184 208 172 98 3478
Fragments arranged by size:
3478 1609 208 184 172 98
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5749
PvuII CAG'CTG
Cuts at: 2430 2430
Size: 5749
SacI G_AGCT'C
Cuts at: 2337 2337
Size: 5749
SalI G'TCGA_C
Cuts at: 1936 3091 1936
Size: 1155 4594
SbfI CC_TGCA'GG
Cuts at: 2439 3101 2439
Size: 662 5087
ScaI AGT'ACT
Cuts at: 3997 5709 3997
Size: 1712 4037
SfcI C'TryA_G
Cuts at: 826 1045 2435 2619 2827 2999 3097 4232 4913
Size: 219 1390 184 208 172 98 1135 681
Cuts at: 4913 5104 826
Size: 191 1471
Fragments arranged by size:
1471 1390 1135 681 219 208 191 184
172 98
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5749
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5749
SmaI CCC'GGG
Cuts at: 1872 2088 2465 2517 2812 3076 1872
Size: 216 377 52 295 264 4545
Fragments arranged by size:
4545 377 295 264 216 52
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3853 4754 5016 820
Size: 197 926 1910 901 262 1553
Fragments arranged by size:
1910 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3540 493
Size: 3047 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5749
SphI G_CATG'C
Cuts at: 1132 3107 1132
Size: 1975 3774
SspI AAT'ATT
Cuts at: 5 52 3673 5
Size: 47 3621 2081
Fragments arranged by size:
3621 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5749
StyI C'CwwG_G
Cuts at: 513 1065 2913 3188 513
Size: 552 1848 275 3074
Fragments arranged by size:
3074 1848 552 275
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3776 3793 3946 4131 4576 325
Size: 1255 2196 17 153 185 445 1498
Fragments arranged by size:
2196 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2016 2750 3995
Size: 274 80 33 51 1443 39 734 1245
Cuts at: 3995 5707 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1245 734 274 138 80 51
39 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5709 5741 5709
Size: 32 5717
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5749
XbaI T'CTAG_A
Cuts at: 3085 3085
Size: 5749
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3016 3016
Size: 5749
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5749
XmaI C'CCGG_G
Cuts at: 1870 2086 2463 2515 2810 3074 1870
Size: 216 377 52 295 264 4545
Fragments arranged by size:
4545 377 295 264 216 52
XmnI GAAnn'nnTTC
Cuts at: 3878 3878
Size: 5749
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3557 276
Size: 53 83 186 936 2023 2468
Fragments arranged by size:
2468 2023 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BbeI BbvCI BfrBI BplI
BsiWI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI
KasI NarI NruI NsiI PacI PmlI PspXI PsrI
RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI