Restriction map for
pFN21AE2732
(Circular) (Six-base) MAPSORT of: pFN21AE2732.seq Check: 4450 from: 1 to: 9529
pFN21AE2732 9529 bp
With 182 enzymes: SgfI *
October 12, 2018 18:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4816 6089 4816
Size: 1273 8256
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7339 278
Size: 53 83 186 936 5803 2468
Fragments arranged by size:
5803 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2913 3559 3594 6093 6872 1937
Size: 976 646 35 2499 779 4594
Fragments arranged by size:
4594 2499 976 779 646 35
Acc65I G'GTAC_C
Cuts at: 2249 3415 5464 5928 6850 2249
Size: 1166 2049 464 922 4928
Fragments arranged by size:
4928 2049 1166 922 464
AclI AA'CG_TT
Cuts at: 1563 7656 8029 1563
Size: 6093 373 3063
Fragments arranged by size:
6093 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2949 3110 3626 3836 3917 4001 4427 4994
Size: 1317 161 516 210 81 84 426 567
Cuts at: 4994 6293 6558 6741 7592 8976 9244 1632
Size: 1299 265 183 851 1384 268 1917
Fragments arranged by size:
1917 1384 1317 1299 851 567 516 426
268 265 210 183 161 84 81
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 9332
AflIII A'CryG_T
Cuts at: 1203 5034 6316 8428 9405 1203
Size: 3831 1282 2112 977 1327
Fragments arranged by size:
3831 2112 1327 1282 977
AgeI A'CCGG_T
Cuts at: 8360 8360
Size: 9529
AhdI GACnn_n'nnGTC
Cuts at: 2655 3457 4938 8258 2655
Size: 802 1481 3320 3926
Fragments arranged by size:
3926 3320 1481 802
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 9529
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3936 1184
Size: 2752 6777
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3834 3866 5021 5053 3834
Size: 32 1155 32 8310
Fragments arranged by size:
8310 1155 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2606 4972 7272 8844 2606
Size: 2366 2300 1572 3291
Fragments arranged by size:
3291 2366 2300 1572
ApaLI G'TGCA_C
Cuts at: 7586 8742 7586
Size: 1156 8373
ApoI r'AATT_y
Cuts at: 1493 3256 3719 4195 4304 4331 4496 4566 5048
Size: 1763 463 476 109 27 165 70 482
Cuts at: 5048 6838 7064 7216 9325 1493
Size: 1790 226 152 2109 1697
Fragments arranged by size:
2109 1790 1763 1697 482 476 463 226
165 152 109 70 27
AseI AT'TA_AT
Cuts at: 160 1032 2889 6431 8083 160
Size: 872 1857 3542 1652 1606
Fragments arranged by size:
3542 1857 1652 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9529
AvaI C'yCGr_G
Cuts at: 1870 1943 2117 2135 6854 1870
Size: 73 174 18 4719 4545
Fragments arranged by size:
4719 4545 174 73 18
AvrII C'CTAG_G
Cuts at: 3410 3410
Size: 9529
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2952 2985 2952
Size: 33 9496
BamHI G'GATC_C
Cuts at: 4128 6859 7226 4128
Size: 2731 367 6431
Fragments arranged by size:
6431 2731 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2249 3415 4757 5464 5928
Size: 325 217 627 462 1166 1342 707 464
Cuts at: 5928 6401 6532 6850 8305 618
Size: 473 131 318 1455 1842
Fragments arranged by size:
1842 1455 1342 1166 707 627 473 464
462 325 318 217 131
BanII G_rGCy'C
Cuts at: 1391 4405 4416 4735 6234 6848 1391
Size: 3014 11 319 1499 614 4072
Fragments arranged by size:
4072 3014 1499 614 319 11
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2217 928
Size: 971 318 8240
Fragments arranged by size:
8240 971 318
BbvCI CC'TCA_GC
Cuts at: 2570 4614 5058 2570
Size: 2044 444 7041
Fragments arranged by size:
7041 2044 444
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4709 4743 6073 6107 7720 7754 1903
Size: 34 2772 34 1330 34 1613 34 3678
Fragments arranged by size:
3678 2772 1613 1330 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 7421 8631 7421
Size: 1210 8319
BclI T'GATC_A
Cuts at: 1548 2470 1548
Size: 922 8607
BfrBI ATG'CAT
Cuts at: 4985 5379 4985
Size: 394 9135
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 8140 136
Size: 107 122 71 631 605 6468 1525
Fragments arranged by size:
6468 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 9524 9524
Size: 9529
BlpI GC'TnA_GC
Cuts at: 2592 6946 2592
Size: 4354 5175
Bme1580I G_kGCm'C
Cuts at: 1790 2168 2613 3239 3676 4760 5668 7590 8746
Size: 378 445 626 437 1084 908 1922 1156
Cuts at: 8746 1790
Size: 2573
Fragments arranged by size:
2573 1922 1156 1084 908 626 445 437
378
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 9529
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4508 6449 7260
Size: 416 53 83 399 24 3091 1941 811
Cuts at: 7260 8218 442
Size: 958 1753
Fragments arranged by size:
3091 1941 1753 958 811 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9529
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3556 3945 4055 5499 6610 8189 1239
Size: 2317 389 110 1444 1111 1579 2579
Fragments arranged by size:
2579 2317 1579 1444 1111 389 110
Bpu10I CC'TnA_GC
Cuts at: 2570 4614 5058 2570
Size: 2044 444 7041
Fragments arranged by size:
7041 2044 444
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3506 3961 4137 5268 5371 5740 6031 6770 7654
Size: 455 176 1131 103 369 291 739 884
Cuts at: 7654 8519 8817 3506
Size: 865 298 4218
Fragments arranged by size:
4218 1131 884 865 739 455 369 298
291 176 103
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2114 3055 3910 4515 8192 882
Size: 617 615 941 855 605 3677 2219
Fragments arranged by size:
3677 2219 941 855 617 615 605
BsaAI yAC'GTr
Cuts at: 493 1204 3677 7320 493
Size: 711 2473 3643 2702
Fragments arranged by size:
3643 2702 2473 711
BsaBI GATnn'nnATC
Cuts at: 2550 4701 9523 2550
Size: 2151 4822 2556
Fragments arranged by size:
4822 2556 2151
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 7336 7718 8353 275
Size: 53 83 186 936 5803 382 635 1451
Fragments arranged by size:
5803 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2110 3418 7962 8360 8634 8781 9192 2110
Size: 1308 4544 398 274 147 411 2447
Fragments arranged by size:
4544 2447 1308 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 9304
Fragments arranged by size:
9304 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2025 2087 3797 3824 4245 4631 5783 5811
Size: 839 62 1710 27 421 386 1152 28
Cuts at: 5811 5956 6779 1186
Size: 145 823 3936
Fragments arranged by size:
3936 1710 1152 839 823 421 386 145
62 28 27
BseYI C'CCAG_C
Cuts at: 2261 3519 4752 5618 6301 8732 9228 2261
Size: 1258 1233 866 683 2431 496 2562
Fragments arranged by size:
2562 2431 1258 1233 866 683 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2616 3194 3773 1163
Size: 573 880 578 579 6919
Fragments arranged by size:
6919 880 579 578 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3700 6582 6979 7740 8768 1540
Size: 275 186 1699 2882 397 761 1028 2301
Fragments arranged by size:
2882 2301 1699 1028 761 397 275 186
BsiHKAI G_wGCw'C
Cuts at: 2495 3529 3542 4416 6234 6654 7590 7675 8746
Size: 1034 13 874 1818 420 936 85 1071
Cuts at: 8746 2495
Size: 3278
Fragments arranged by size:
3278 1818 1071 1034 936 874 420 85
13
BsmI GAATG_Cn'
Cuts at: 2362 4278 5381 7040 7133 2362
Size: 1916 1103 1659 93 4758
Fragments arranged by size:
4758 1916 1659 1103 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2168 2495 2613 3239 3529 3542 3676
Size: 399 378 327 118 626 290 13 134
Cuts at: 3676 4405 4416 4735 4760 5668 6234 6654 6848
Size: 729 11 319 25 908 566 420 194
Cuts at: 6848 7590 7675 8746 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 908 742 729 626 566 420
399 378 327 319 290 194 134 118
85 25 13 11
BspEI T'CCGG_A
Cuts at: 9192 9192
Size: 9529
BspHI T'CATG_A
Cuts at: 3025 7418 9148 3025
Size: 4393 1730 3406
Fragments arranged by size:
4393 3406 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2638 3052 3853 3928 4816 5323 6089 6884
Size: 1794 414 801 75 888 507 766 795
Cuts at: 6884 844
Size: 3489
Fragments arranged by size:
3489 1794 888 801 795 766 507 414
75
BsrBI CCG'CTC
Cuts at: 1394 1823 7416 1394
Size: 429 5593 3507
Fragments arranged by size:
5593 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2593 3113 4263 5017 5115 6348 8024
Size: 1183 1345 520 1150 754 98 1233 1676
Cuts at: 8024 8198 65
Size: 174 1396
Fragments arranged by size:
1676 1396 1345 1233 1183 1150 754 520
174 98
BsrFI r'CCGG_y
Cuts at: 1700 1810 4945 8173 8360 1700
Size: 110 3135 3228 187 2869
Fragments arranged by size:
3228 3135 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2485 6075 6249 96
Size: 2389 3590 174 3376
Fragments arranged by size:
3590 3376 2389 174
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9529
BssSI C'ACGA_G
Cuts at: 6549 7589 8601 6549
Size: 1040 1012 7477
Fragments arranged by size:
7477 1040 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 9529
BstBI TT'CG_AA
Cuts at: 3840 6186 8333 3840
Size: 2346 2147 5036
Fragments arranged by size:
5036 2346 2147
BstEII G'GTnAC_C
Cuts at: 1184 6019 1184
Size: 4835 4694
BstXI CCAn_nnnn'nTGG
Cuts at: 6374 6374
Size: 9529
BstYI r'GATC_y
Cuts at: 1972 2439 2519 2551 3727 4081 4128 6859 7226
Size: 467 80 32 1176 354 47 2731 367
Cuts at: 7226 7611 7628 9080 9166 9178 9524 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
2731 1977 1452 1176 467 385 367 354
346 86 80 47 32 17 12
Bsu36I CC'TnA_GG
Cuts at: 4791 4791
Size: 9529
BtgI C'CryG_G
Cuts at: 513 1065 2067 3822 5560 5763 6568 513
Size: 552 1002 1755 1738 203 805 3474
Fragments arranged by size:
3474 1755 1738 1002 805 552 203
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4143 4936 6285 487
Size: 683 56 2917 793 1349 3731
Fragments arranged by size:
3731 2917 1349 793 683 56
BtsI GCAGTG_nn'
Cuts at: 2984 3799 4363 5668 6742 7045 7839 7859 2984
Size: 815 564 1305 1074 303 794 20 4654
Fragments arranged by size:
4654 1305 1074 815 794 564 303 20
ClaI AT'CG_AT
Cuts at: 1554 4695 5704 1554
Size: 3141 1009 5379
Fragments arranged by size:
5379 3141 1009
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9494
DraI TTT'AAA
Cuts at: 2673 4170 6833 2673
Size: 1497 2663 5369
Fragments arranged by size:
5369 2663 1497
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 8536 809
Size: 507 7220 1802
Fragments arranged by size:
7220 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3275 5383 6111
Size: 54 1425 50 275 18 1445 2108 728
Cuts at: 6111 6579 6976 7865 9494 8
Size: 468 397 889 1629 43
Fragments arranged by size:
2108 1629 1445 1425 889 728 468 397
275 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6579 6976 1537
Size: 275 4767 397 4090
Fragments arranged by size:
4767 4090 397 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3129 4071 4421 4616 6282 7459 1353
Size: 1776 942 350 195 1666 1177 3423
Fragments arranged by size:
3423 1776 1666 1177 942 350 195
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 8116 8490 8636 387
Size: 1064 138 6527 374 146 1280
Fragments arranged by size:
6527 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4414 6232 4414
Size: 1818 7711
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2949 3110 3556 3626 3836 3917 3945
Size: 393 1317 161 446 70 210 81 28
Cuts at: 3945 4001 4055 4427 4994 5499 6293 6558 6610
Size: 56 54 372 567 505 794 265 52
Cuts at: 6610 6741 7592 8189 8976 9244 1239
Size: 131 851 597 787 268 1524
Fragments arranged by size:
1524 1317 851 794 787 597 567 505
446 393 372 268 265 210 161 131
81 70 56 54 52 28
EcoNI CCTnn'n_nnAGG
Cuts at: 3667 5415 3667
Size: 1748 7781
EcoO109I rG'GnC_Cy
Cuts at: 3976 4477 5739 5879 3976
Size: 501 1262 140 7626
Fragments arranged by size:
7626 1262 501 140
EcoRI G'AATT_C
Cuts at: 4496 6838 7216 4496
Size: 2342 378 6809
Fragments arranged by size:
6809 2342 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4432 6040 4432
Size: 1608 7921
FspI TGC'GCA
Cuts at: 4863 8035 4863
Size: 3172 6357
HaeII r_GCGC'y
Cuts at: 1678 8676 1678
Size: 6998 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2111 2143 2641 2673 3443
Size: 32 135 32 539 32 498 32 770
Cuts at: 3443 3458 3475 3490 4919 4924 4951 4956 8170
Size: 15 17 15 1429 5 27 5 3214
Cuts at: 8170 8202 8244 8276 1373
Size: 32 42 32 2626
Fragments arranged by size:
3214 2626 1429 770 539 498 135 42
32 32 32 32 32 32 27 17
15 15 5 5
HincII GTy'rAC
Cuts at: 669 1657 1938 2292 2914 4807 6094 6873 7119
Size: 988 281 354 622 1893 1287 779 246
Cuts at: 7119 669
Size: 3079
Fragments arranged by size:
3079 1893 1287 988 779 622 354 281
246
HindIII A'AGCT_T
Cuts at: 748 2594 2705 3482 4250 4884 4959 5206 748
Size: 1846 111 777 768 634 75 247 5071
Fragments arranged by size:
5071 1846 777 768 634 247 111 75
HpaI GTT'AAC
Cuts at: 7119 7119
Size: 9529
KpnI G_GTAC'C
Cuts at: 2253 3419 5468 5932 6854 2253
Size: 1166 2049 464 922 4928
Fragments arranged by size:
4928 2049 1166 922 464
MfeI C'AATT_G
Cuts at: 5307 7128 5307
Size: 1821 7708
MluI A'CGCG_T
Cuts at: 9405 9405
Size: 9529
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3061 3345 4389 5571 8643 8827 1305
Size: 1756 284 1044 1182 3072 184 2007
Fragments arranged by size:
3072 2007 1756 1182 1044 284 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3277 5385 6113 10
Size: 54 1425 343 1445 2108 728 3426
Fragments arranged by size:
3426 2108 1445 1425 728 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4826 6257 6372 6573 6614 6645 7487
Size: 648 3660 1431 115 201 41 31 842
Cuts at: 7487 7846 8005 518
Size: 359 159 2042
Fragments arranged by size:
3660 2042 1431 842 648 359 201 159
115 41 31
MspA1I CmG'CkG
Cuts at: 1606 2018 3955 4652 4752 4972 6945 7622 8770
Size: 412 1937 697 100 220 1973 677 1148
Cuts at: 8770 9015 1606
Size: 245 2120
Fragments arranged by size:
2120 1973 1937 1148 697 677 412 245
220 100
NcoI C'CATG_G
Cuts at: 513 1065 2067 3822 5560 6568 513
Size: 552 1002 1755 1738 1008 3474
Fragments arranged by size:
3474 1755 1738 1008 1002 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 9529
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9529
NotI GC'GGCC_GC
Cuts at: 6976 6976
Size: 9529
NsiI A_TGCA'T
Cuts at: 4987 5381 4987
Size: 394 9135
NspI r_CATG'y
Cuts at: 1132 1229 2290 2822 4825 5038 5542 6320 6887
Size: 97 1061 532 2003 213 504 778 567
Cuts at: 6887 8432 1132
Size: 1545 2229
Fragments arranged by size:
2229 2003 1545 1061 778 567 532 504
213 97
PasI CC'CwG_GG
Cuts at: 1348 6511 1348
Size: 5163 4366
PciI A'CATG_T
Cuts at: 5034 6316 8428 5034
Size: 1282 2112 6135
Fragments arranged by size:
6135 2112 1282
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 9529
PfoI T'CCnGG_A
Cuts at: 2992 3162 4527 2992
Size: 170 1365 7994
Fragments arranged by size:
7994 1365 170
PmeI GTTT'AAAC
Cuts at: 6833 6833
Size: 9529
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3921 3953 7598 7630 9111 9143 3921
Size: 32 3645 32 1481 32 4307
Fragments arranged by size:
4307 3645 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3976 4477 5739 5879 3976
Size: 501 1262 140 7626
Fragments arranged by size:
7626 1262 501 140
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 9529
PsiI TTA'TAA
Cuts at: 7099 7099
Size: 9529
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3998 4030 3998
Size: 32 9497
PstI C_TGCA'G
Cuts at: 830 4026 4657 6169 6881 830
Size: 3196 631 1512 712 3478
Fragments arranged by size:
3478 3196 1512 712 631
PvuI CG_AT'CG
Cuts at: 2001 3700 2001
Size: 1699 7830
PvuII CAG'CTG
Cuts at: 3955 4652 4752 4972 3955
Size: 697 100 220 8512
Fragments arranged by size:
8512 697 220 100
SacI G_AGCT'C
Cuts at: 4416 6234 4416
Size: 1818 7711
SalI G'TCGA_C
Cuts at: 1936 2912 6092 6871 1936
Size: 976 3180 779 4594
Fragments arranged by size:
4594 3180 976 779
SapI GCTCTTCn'nnn_
Cuts at: 3129 4421 3129
Size: 1292 8237
SbfI CC_TGCA'GG
Cuts at: 6881 6881
Size: 9529
ScaI AGT'ACT
Cuts at: 4007 5096 6266 7777 9489 4007
Size: 1089 1170 1511 1712 4047
Fragments arranged by size:
4047 1712 1511 1170 1089
SfcI C'TryA_G
Cuts at: 826 1045 2581 2619 4022 4139 4287 4653 6165
Size: 219 1536 38 1403 117 148 366 1512
Cuts at: 6165 6877 8012 8693 8884 826
Size: 712 1135 681 191 1471
Fragments arranged by size:
1536 1512 1471 1403 1135 712 681 366
219 191 148 117 38
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9529
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9529
SmaI CCC'GGG
Cuts at: 1872 6856 1872
Size: 4984 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3485 3940 4152 5283 5350 5755
Size: 197 926 1542 455 212 1131 67 405
Cuts at: 5755 6010 6749 7633 8534 8796 820
Size: 255 739 884 901 262 1553
Fragments arranged by size:
1553 1542 1131 926 901 884 739 455
405 262 255 212 197 67
SnaBI TAC'GTA
Cuts at: 493 7320 493
Size: 6827 2702
SpeI A'CTAG_T
Cuts at: 152 4340 4843 152
Size: 4188 503 4838
Fragments arranged by size:
4838 4188 503
SphI G_CATG'C
Cuts at: 1132 2822 4825 5542 6887 1132
Size: 1690 2003 717 1345 3774
Fragments arranged by size:
3774 2003 1690 1345 717
SspI AAT'ATT
Cuts at: 5 52 6183 7453 5
Size: 47 6131 1270 2081
Fragments arranged by size:
6131 2081 1270 47
StuI AGG'CCT
Cuts at: 1517 6634 1517
Size: 5117 4412
StyI C'CwwG_G
Cuts at: 513 1065 2067 2575 3410 3822 5560 6568 6635
Size: 552 1002 508 835 412 1738 1008 67
Cuts at: 6635 6968 513
Size: 333 3074
Fragments arranged by size:
3074 1738 1008 1002 835 552 508 412
333 67
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2138 2322 2504 7556 7573 7726 7911
Size: 1255 558 184 182 5052 17 153 185
Cuts at: 7911 8356 325
Size: 445 1498
Fragments arranged by size:
5052 1498 1255 558 445 185 184 182
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2485 3090 4005
Size: 274 80 33 51 1443 508 605 915
Cuts at: 4005 5094 6075 6249 6264 7775 9487 96
Size: 1089 981 174 15 1511 1712 138
Fragments arranged by size:
1712 1511 1443 1089 981 915 605 508
274 174 138 80 51 33 15
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2382 2414 9489 9521 2382
Size: 32 7075 32 2390
Fragments arranged by size:
7075 2390 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2125 3564 1316
Size: 809 1439 7281
Fragments arranged by size:
7281 1439 809
XbaI T'CTAG_A
Cuts at: 2206 6865 2206
Size: 4659 4870
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3732 5023 6488 6768 3732
Size: 1291 1465 280 6493
Fragments arranged by size:
6493 1465 1291 280
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 9529
XmaI C'CCGG_G
Cuts at: 1870 6854 1870
Size: 4984 4545
XmnI GAAnn'nnTTC
Cuts at: 4253 5154 6182 7658 4253
Size: 901 1028 1476 6124
Fragments arranged by size:
6124 1476 1028 901
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7337 276
Size: 53 83 186 936 5803 2468
Fragments arranged by size:
5803 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PsrI PstI PvuI PvuII SacI SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI BbeI BplI BsiWI BsmBI BstZ17I
DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PmlI PspOMI PspXI RsrII SacII SanDI
SexAI SfiI SfoI SrfI SwaI