Restriction map for
pFN21AE2727
(Circular) (Six-base) MAPSORT of: pFN21AE2727.seq Check: 5989 from: 1 to: 8347
pFN21AE2727 8347 bp
With 182 enzymes: SgfI *
August 16, 2021 15:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4631 5042 4631
Size: 411 7936
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6157 278
Size: 53 83 186 936 4621 2468
Fragments arranged by size:
4621 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5690 1937
Size: 3753 4594
Acc65I G'GTAC_C
Cuts at: 2634 3558 5668 2634
Size: 924 2110 5313
Fragments arranged by size:
5313 2110 924
AclI AA'CG_TT
Cuts at: 1563 6474 6847 1563
Size: 4911 373 3063
Fragments arranged by size:
4911 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4117 5468 6410 7794 8062 1632
Size: 2485 1351 942 1384 268 1917
Fragments arranged by size:
2485 1917 1384 1351 942 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8150
AflIII A'CryG_T
Cuts at: 1203 4791 7246 8223 1203
Size: 3588 2455 977 1327
Fragments arranged by size:
3588 2455 1327 977
AgeI A'CCGG_T
Cuts at: 2637 7178 2637
Size: 4541 3806
AhdI GACnn_n'nnGTC
Cuts at: 3442 7076 3442
Size: 3634 4713
AleI CACnn'nnGTG
Cuts at: 1166 2549 4039 1166
Size: 1383 1490 5474
Fragments arranged by size:
5474 1490 1383
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3635 1184
Size: 2451 5896
AlwNI CAG_nnn'CTG
Cuts at: 2413 4027 4501 4570 4980 5518 6090 7662 2413
Size: 1614 474 69 410 538 572 1572 3098
Fragments arranged by size:
3098 1614 1572 572 538 474 410 69
ApaI G_GGCC'C
Cuts at: 2387 4081 4538 4758 5023 2387
Size: 1694 457 220 265 5711
Fragments arranged by size:
5711 1694 457 265 220
ApaLI G'TGCA_C
Cuts at: 6404 7560 6404
Size: 1156 7191
ApoI r'AATT_y
Cuts at: 1493 3119 5207 5656 5882 6034 8143 1493
Size: 1626 2088 449 226 152 2109 1697
Fragments arranged by size:
2109 2088 1697 1626 449 226 152
AscI GG'CGCG_CC
Cuts at: 4335 4335
Size: 8347
AseI AT'TA_AT
Cuts at: 160 1032 4194 6901 160
Size: 872 3162 2707 1606
Fragments arranged by size:
3162 2707 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8347
AvaI C'yCGr_G
Cuts at: 1870 1943 2997 3665 4538 4657 5321 5672 1870
Size: 73 1054 668 873 119 664 351 4545
Fragments arranged by size:
4545 1054 873 668 664 351 119 73
BamHI G'GATC_C
Cuts at: 2034 2296 5677 6044 2034
Size: 262 3381 367 4337
Fragments arranged by size:
4337 3381 367 262
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2011 2118 2543 2634 3403
Size: 325 217 627 224 107 425 91 769
Cuts at: 3403 3458 3558 3572 4299 4305 4426 5030 5668
Size: 55 100 14 727 6 121 604 638
Cuts at: 5668 7123 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 769 727 638 627 604 425
325 224 217 121 107 100 91 55
14 6
BanII G_rGCy'C
Cuts at: 1391 2387 2621 3084 3167 3498 4081 4155 4538
Size: 996 234 463 83 331 583 74 383
Cuts at: 4538 4758 4857 4995 5023 5300 5666 1391
Size: 220 99 138 28 277 366 4072
Fragments arranged by size:
4072 996 583 463 383 366 331 277
234 220 138 99 83 74 28
BbeI G_GCGC'C
Cuts at: 2122 3407 3576 4303 4430 2122
Size: 1285 169 727 127 6039
Fragments arranged by size:
6039 1285 727 169 127
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2063 2792 2958 4241 4894 928
Size: 971 164 729 166 1283 653 4381
Fragments arranged by size:
4381 1283 971 729 653 166 164
BbvCI CC'TCA_GC
Cuts at: 3175 3350 3524 3766 3822 3175
Size: 175 174 242 56 7700
Fragments arranged by size:
7700 242 175 174 56
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6538 6572 1903
Size: 34 4601 34 3678
Fragments arranged by size:
4601 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4898 6239 7449 4898
Size: 1341 1210 5796
Fragments arranged by size:
5796 1341 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8347
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2165 2736 2882
Size: 107 122 71 631 605 493 571 146
Cuts at: 2882 4263 6958 136
Size: 1381 2695 1525
Fragments arranged by size:
2695 1525 1381 631 605 571 493 146
122 107 71
BglII A'GATC_T
Cuts at: 2043 4923 8342 2043
Size: 2880 3419 2048
Fragments arranged by size:
3419 2880 2048
BlpI GC'TnA_GC
Cuts at: 2886 5764 2886
Size: 2878 5469
Bme1580I G_kGCm'C
Cuts at: 1790 2347 2387 2546 3703 3721 4081 4538 4758
Size: 557 40 159 1157 18 360 457 220
Cuts at: 4758 5008 5023 5033 6408 7564 1790
Size: 250 15 10 1375 1156 2573
Fragments arranged by size:
2573 1375 1157 1156 557 457 360 250
220 159 40 18 15 10
BmgBI CAC'GTC
Cuts at: 1318 3511 4792 4957 5457 1318
Size: 2193 1281 165 500 4208
Fragments arranged by size:
4208 2193 1281 500 165
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2478 2547 5038
Size: 416 53 83 399 24 1061 69 2491
Cuts at: 5038 6078 7036 442
Size: 1040 958 1753
Fragments arranged by size:
2491 1753 1061 1040 958 416 399 83
69 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8347
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2618 5452 2618
Size: 2834 5513
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2378 2783 3700 3830 3953 4463 5549 7007
Size: 1139 405 917 130 123 510 1086 1458
Cuts at: 7007 1239
Size: 2579
Fragments arranged by size:
2579 1458 1139 1086 917 510 405 130
123
Bpu10I CC'TnA_GC
Cuts at: 2914 3175 3350 3524 3766 3822 4502 5270 5582
Size: 261 175 174 242 56 680 768 312
Cuts at: 5582 2914
Size: 5679
Fragments arranged by size:
5679 768 680 312 261 242 175 174
56
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3056 6472 7337 7635 3056
Size: 3416 865 298 3768
Fragments arranged by size:
3768 3416 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2936 3119 7010 882
Size: 617 1437 183 3891 2219
Fragments arranged by size:
3891 2219 1437 617 183
BsaAI yAC'GTr
Cuts at: 493 1204 6138 493
Size: 711 4934 2702
Fragments arranged by size:
4934 2702 711
BsaBI GATnn'nnATC
Cuts at: 4766 8341 4766
Size: 3575 4772
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2119 3297 3404 3443
Size: 53 83 186 936 586 1178 107 39
Cuts at: 3443 3573 3990 4238 4300 4427 6154 6536 7171
Size: 130 417 248 62 127 1727 382 635
Cuts at: 7171 275
Size: 1451
Fragments arranged by size:
1727 1451 1178 936 635 586 417 382
248 186 130 127 107 83 62 53
39
BsaWI w'CCGG_w
Cuts at: 2586 2637 6780 7178 7452 7599 8010 2586
Size: 51 4143 398 274 147 411 2923
Fragments arranged by size:
4143 2923 411 398 274 147 51
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3599 3629 4157 4187 1178
Size: 30 165 30 2196 30 528 30 5338
Fragments arranged by size:
5338 2196 528 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2819 2891 3839 3917 3920 4163 4178 5063
Size: 1633 72 948 78 3 243 15 885
Cuts at: 5063 1186
Size: 4470
Fragments arranged by size:
4470 1633 948 885 243 78 72 15
3
BseYI C'CCAG_C
Cuts at: 2368 3469 3535 4225 4243 4750 5301 5488 7550
Size: 1101 66 690 18 507 551 187 2062
Cuts at: 7550 8046 2368
Size: 496 2669
Fragments arranged by size:
2669 2062 1101 690 551 507 496 187
66 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2047 2462 5302 1163
Size: 573 311 415 2840 4208
Fragments arranged by size:
4208 2840 573 415 311
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2257 2494 5797 6558 7586 1540
Size: 275 186 256 237 3303 761 1028 2301
Fragments arranged by size:
3303 2301 1028 761 275 256 237 186
BsiHKAI G_wGCw'C
Cuts at: 2450 3084 3167 3498 4974 6408 6493 7564 2450
Size: 634 83 331 1476 1434 85 1071 3233
Fragments arranged by size:
3233 1476 1434 1071 634 331 85 83
BsmI GAATG_Cn'
Cuts at: 5858 5951 5858
Size: 93 8254
BsmBI CGTCTCn'nnnn_
Cuts at: 5044 5044
Size: 8347
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2347 2387 2450 2546 2621 3084 3167
Size: 399 557 40 63 96 75 463 83
Cuts at: 3167 3498 3703 3721 4081 4155 4538 4758 4857
Size: 331 205 18 360 74 383 220 99
Cuts at: 4857 4974 4995 5008 5023 5033 5300 5666 6408
Size: 117 21 13 15 10 267 366 742
Cuts at: 6408 6493 7564 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 557 463 399 383 366
360 331 267 220 205 117 99 96
85 83 75 74 63 40 21 18
15 13 10
BspEI T'CCGG_A
Cuts at: 2586 8010 2586
Size: 5424 2923
BspHI T'CATG_A
Cuts at: 6236 7966 6236
Size: 1730 6617
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2069 2421 3696 4383 4631 4926 5042 5637
Size: 1225 352 1275 687 248 295 116 595
Cuts at: 5637 5702 844
Size: 65 3489
Fragments arranged by size:
3489 1275 1225 687 595 352 295 248
116 65
BsrBI CCG'CTC
Cuts at: 1394 1823 3646 3946 4506 5359 5533 5589 6234
Size: 429 1823 300 560 853 174 56 645
Cuts at: 6234 1394
Size: 3507
Fragments arranged by size:
3507 1823 853 645 560 429 300 174
56
BsrDI GCAATG_nn'
Cuts at: 65 1248 3225 5121 6842 7016 65
Size: 1183 1977 1896 1721 174 1396
Fragments arranged by size:
1977 1896 1721 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2252 2493 2637 3312 4302 4339 4423
Size: 110 442 241 144 675 990 37 84
Cuts at: 4423 4647 6991 7178 1700
Size: 224 2344 187 2869
Fragments arranged by size:
2869 2344 990 675 442 241 224 187
144 110 84 37
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8347
BssHII G'CGCG_C
Cuts at: 1925 3549 4335 1925
Size: 1624 786 5937
Fragments arranged by size:
5937 1624 786
BssSI C'ACGA_G
Cuts at: 2903 4409 5090 6407 7419 2903
Size: 1506 681 1317 1012 3831
Fragments arranged by size:
3831 1506 1317 1012 681
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2413 794
Size: 1619 6728
BstBI TT'CG_AA
Cuts at: 7151 7151
Size: 8347
BstEII G'GTnAC_C
Cuts at: 1184 2176 2648 4342 1184
Size: 992 472 1694 5189
Fragments arranged by size:
5189 1694 992 472
BstXI CCAn_nnnn'nTGG
Cuts at: 2554 2566 4354 5059 2554
Size: 12 1788 705 5842
Fragments arranged by size:
5842 1788 705 12
BstYI r'GATC_y
Cuts at: 1972 2034 2043 2089 2296 4923 5677 6044 6429
Size: 62 9 46 207 2627 754 367 385
Cuts at: 6429 6446 7898 7984 7996 8342 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
2627 1977 1452 754 385 367 346 207
86 62 46 17 12 9
Bsu36I CC'TnA_GG
Cuts at: 4859 4859
Size: 8347
BtgI C'CryG_G
Cuts at: 513 1065 2004 2148 2172 2205 2559 3343 4845
Size: 552 939 144 24 33 354 784 1502
Cuts at: 4845 4865 513
Size: 20 3995
Fragments arranged by size:
3995 1502 939 784 552 354 144 33
24 20
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2208 487
Size: 683 56 982 6626
Fragments arranged by size:
6626 982 683 56
BtsI GCAGTG_nn'
Cuts at: 2148 3910 5552 5863 6657 6677 2148
Size: 1762 1642 311 794 20 3818
Fragments arranged by size:
3818 1762 1642 794 311 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8347
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8312
DraI TTT'AAA
Cuts at: 5651 5651
Size: 8347
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2819 3038 7354 809
Size: 507 1503 219 4316 1802
Fragments arranged by size:
4316 1802 1503 507 219
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2145 2254 2491
Size: 54 1425 50 275 18 315 109 237
Cuts at: 2491 2529 2556 3726 3975 4056 4546 4842 5094
Size: 38 27 1170 249 81 490 296 252
Cuts at: 5094 5571 5794 6683 8312 8
Size: 477 223 889 1629 43
Fragments arranged by size:
1629 1425 1170 889 490 477 315 296
275 252 249 237 223 109 81 54
50 43 38 27 18
EagI C'GGCC_G
Cuts at: 1537 1812 2254 2491 5794 1537
Size: 275 442 237 3303 4090
Fragments arranged by size:
4090 3303 442 275 237
EarI CTCTTCn'nnn_
Cuts at: 1353 4140 4811 6277 1353
Size: 2787 671 1466 3423
Fragments arranged by size:
3423 2787 1466 671
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2954 4235 6934 7308 7454 387
Size: 1064 138 1365 1281 2699 374 146 1280
Fragments arranged by size:
2699 1365 1281 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3082 3165 3496 3082
Size: 83 331 7933
Fragments arranged by size:
7933 331 83
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2378 2783 3700 3830 3953 4117 4463
Size: 393 746 405 917 130 123 164 346
Cuts at: 4463 5468 5549 6410 7007 7794 8062 1239
Size: 1005 81 861 597 787 268 1524
Fragments arranged by size:
1524 1005 917 861 787 746 597 405
393 346 268 164 130 123 81
EcoNI CCTnn'n_nnAGG
Cuts at: 3149 3588 3668 5345 3149
Size: 439 80 1677 6151
Fragments arranged by size:
6151 1677 439 80
EcoO109I rG'GnC_Cy
Cuts at: 2100 2383 2384 2410 3031 3040 3142 3580 3673
Size: 283 1 26 621 9 102 438 93
Cuts at: 3673 3777 4078 4205 4249 4498 4534 4535 4754
Size: 104 301 127 44 249 36 1 219
Cuts at: 4754 5019 5578 2100
Size: 265 559 4869
Fragments arranged by size:
4869 621 559 438 301 283 265 249
219 127 104 102 93 44 36 26
9 1 1
EcoRI G'AATT_C
Cuts at: 3119 5207 5656 6034 3119
Size: 2088 449 378 5432
Fragments arranged by size:
5432 2088 449 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3050 5413 5488 3050
Size: 2363 75 5909
Fragments arranged by size:
5909 2363 75
FseI GG_CCGG'CC
Cuts at: 2256 2256
Size: 8347
FspI TGC'GCA
Cuts at: 6853 6853
Size: 8347
HaeII r_GCGC'y
Cuts at: 1678 2122 3407 3576 4303 4430 4570 5518 7494
Size: 444 1285 169 727 127 140 948 1976
Cuts at: 7494 1678
Size: 2531
Fragments arranged by size:
2531 1976 1285 948 727 444 169 140
127
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2991 3023 3095 3127 3428
Size: 32 135 32 1419 32 72 32 301
Cuts at: 3428 3460 3601 3633 6988 7020 7062 7094 1373
Size: 32 141 32 3355 32 42 32 2626
Fragments arranged by size:
3355 2626 1419 301 141 135 72 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2080 2859 3877 4741 5113 5691
Size: 988 281 142 779 1018 864 372 578
Cuts at: 5691 5937 669
Size: 246 3079
Fragments arranged by size:
3079 1018 988 864 779 578 372 281
246 142
HindIII A'AGCT_T
Cuts at: 748 3282 748
Size: 2534 5813
HpaI GTT'AAC
Cuts at: 5937 5937
Size: 8347
KasI G'GCGC_C
Cuts at: 2118 3403 3572 4299 4426 2118
Size: 1285 169 727 127 6039
Fragments arranged by size:
6039 1285 727 169 127
KpnI G_GTAC'C
Cuts at: 2638 3562 5672 2638
Size: 924 2110 5313
Fragments arranged by size:
5313 2110 924
MfeI C'AATT_G
Cuts at: 5276 5946 5276
Size: 670 7677
MluI A'CGCG_T
Cuts at: 8223 8223
Size: 8347
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3435 4968 7461 7645 1305
Size: 2130 1533 2493 184 2007
Fragments arranged by size:
2493 2130 2007 1533 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2147 2531 2558 3728 3977
Size: 54 1425 343 315 384 27 1170 249
Cuts at: 3977 4058 4548 4844 5096 10
Size: 81 490 296 252 3261
Fragments arranged by size:
3261 1425 1170 490 384 343 315 296
252 249 81 54 27
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2329 2502 2549 2564 4039 4352
Size: 648 843 320 173 47 15 1475 313
Cuts at: 4352 4955 5126 6305 6664 6823 518
Size: 603 171 1179 359 159 2042
Fragments arranged by size:
2042 1475 1179 843 648 603 359 320
313 173 171 159 47 15
MspA1I CmG'CkG
Cuts at: 1606 2303 3973 4243 4290 4317 4530 4593 4750
Size: 697 1670 270 47 27 213 63 157
Cuts at: 4750 5488 5763 6440 7588 7833 1606
Size: 738 275 677 1148 245 2120
Fragments arranged by size:
2120 1670 1148 738 697 677 275 270
245 213 157 63 47 27
NaeI GCC'GGC
Cuts at: 2254 2495 4425 4649 2254
Size: 241 1930 224 5952
Fragments arranged by size:
5952 1930 241 224
NarI GG'CG_CC
Cuts at: 2119 3404 3573 4300 4427 2119
Size: 1285 169 727 127 6039
Fragments arranged by size:
6039 1285 727 169 127
NcoI C'CATG_G
Cuts at: 513 1065 2004 2148 2559 513
Size: 552 939 144 411 6301
Fragments arranged by size:
6301 939 552 411 144
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8347
NgoMIV G'CCGG_C
Cuts at: 2252 2493 4423 4647 2252
Size: 241 1930 224 5952
Fragments arranged by size:
5952 1930 241 224
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8347
NotI GC'GGCC_GC
Cuts at: 5794 5794
Size: 8347
NspI r_CATG'y
Cuts at: 1132 1229 2501 3521 3616 5705 7250 1132
Size: 97 1272 1020 95 2089 1545 2229
Fragments arranged by size:
2229 2089 1545 1272 1020 97 95
PasI CC'CwG_GG
Cuts at: 1348 2574 2966 1348
Size: 1226 392 6729
Fragments arranged by size:
6729 1226 392
PciI A'CATG_T
Cuts at: 7246 7246
Size: 8347
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2527 2972 5467 1108
Size: 1419 445 2495 3988
Fragments arranged by size:
3988 2495 1419 445
PfoI T'CCnGG_A
Cuts at: 2092 5202 2092
Size: 3110 5237
PmeI GTTT'AAAC
Cuts at: 5651 5651
Size: 8347
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6416 6448 7929 7961 6416
Size: 32 1481 32 6802
Fragments arranged by size:
6802 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2100 2410 3031 3040 3580 4249 2100
Size: 310 621 9 540 669 6198
Fragments arranged by size:
6198 669 621 540 310 9
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8347
PsiI TTA'TAA
Cuts at: 5917 5917
Size: 8347
PspOMI G'GGCC_C
Cuts at: 2383 4077 4534 4754 5019 2383
Size: 1694 457 220 265 5711
Fragments arranged by size:
5711 1694 457 265 220
PspXI vC'TCGA_Gb
Cuts at: 5321 5321
Size: 8347
PstI C_TGCA'G
Cuts at: 830 2308 2409 3048 4923 5699 830
Size: 1478 101 639 1875 776 3478
Fragments arranged by size:
3478 1875 1478 776 639 101
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8347
PvuII CAG'CTG
Cuts at: 2303 3973 4593 4750 5488 2303
Size: 1670 620 157 738 5162
Fragments arranged by size:
5162 1670 738 620 157
RsrII CG'GwC_CG
Cuts at: 2127 2127
Size: 8347
SacI G_AGCT'C
Cuts at: 3084 3167 3498 3084
Size: 83 331 7933
Fragments arranged by size:
7933 331 83
SalI G'TCGA_C
Cuts at: 1936 5689 1936
Size: 3753 4594
SanDI GG'GwC_CC
Cuts at: 3580 4249 3580
Size: 669 7678
SbfI CC_TGCA'GG
Cuts at: 3048 5699 3048
Size: 2651 5696
ScaI AGT'ACT
Cuts at: 6595 8307 6595
Size: 1712 6635
SexAI A'CCwGG_T
Cuts at: 2352 2352
Size: 8347
SfcI C'TryA_G
Cuts at: 826 1045 2304 2405 3044 3394 4919 5695 6830
Size: 219 1259 101 639 350 1525 776 1135
Cuts at: 6830 7511 7702 826
Size: 681 191 1471
Fragments arranged by size:
1525 1471 1259 1135 776 681 639 350
219 191 101
SfoI GGC'GCC
Cuts at: 2120 3405 3574 4301 4428 2120
Size: 1285 169 727 127 6039
Fragments arranged by size:
6039 1285 727 169 127
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8347
SgrAI Cr'CCGG_yG
Cuts at: 1700 4302 4339 1700
Size: 2602 37 5708
Fragments arranged by size:
5708 2602 37
SmaI CCC'GGG
Cuts at: 1872 4540 4659 5674 1872
Size: 2668 119 1015 4545
Fragments arranged by size:
4545 2668 1015 119
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3035 5321 6451 7352 7614 820
Size: 197 926 1092 2286 1130 901 262 1553
Fragments arranged by size:
2286 1553 1130 1092 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 6138 493
Size: 5645 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8347
SphI G_CATG'C
Cuts at: 1132 5705 1132
Size: 4573 3774
SspI AAT'ATT
Cuts at: 5 52 6271 5
Size: 47 6219 2081
Fragments arranged by size:
6219 2081 47
StuI AGG'CCT
Cuts at: 1517 3155 3173 1517
Size: 1638 18 6691
Fragments arranged by size:
6691 1638 18
StyI C'CwwG_G
Cuts at: 513 1065 2004 2148 2547 2559 2613 5786 513
Size: 552 939 144 399 12 54 3173 3074
Fragments arranged by size:
3173 3074 939 552 399 144 54 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2304 2552 2894 3603 4084 5557 6374
Size: 1255 724 248 342 709 481 1473 817
Cuts at: 6374 6391 6544 6729 7174 325
Size: 17 153 185 445 1498
Fragments arranged by size:
1498 1473 1255 817 724 709 481 445
342 248 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3732 5152 6593
Size: 274 80 33 51 1443 1755 1420 1441
Cuts at: 6593 8305 96
Size: 1712 138
Fragments arranged by size:
1755 1712 1443 1441 1420 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 8307 8339 8307
Size: 32 8315
Tth111I GACn'n_nGTC
Cuts at: 1316 2663 1316
Size: 1347 7000
XbaI T'CTAG_A
Cuts at: 5683 5683
Size: 8347
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4078 5059 5104 4078
Size: 981 45 7321
Fragments arranged by size:
7321 981 45
XhoI C'TCGA_G
Cuts at: 1943 5321 1943
Size: 3378 4969
XmaI C'CCGG_G
Cuts at: 1870 4538 4657 5672 1870
Size: 2668 119 1015 4545
Fragments arranged by size:
4545 2668 1015 119
XmnI GAAnn'nnTTC
Cuts at: 2747 3243 6476 2747
Size: 496 3233 4618
Fragments arranged by size:
4618 3233 496
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6155 276
Size: 53 83 186 936 4621 2468
Fragments arranged by size:
4621 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI
PstI PvuI PvuII RsrII SacI SalI SanDI SbfI
ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AvrII BaeI BfrBI BsiWI BstZ17I DraIII
EcoRV FspAI NruI NsiI PacI PmlI PsrI SacII
SapI SfiI SrfI SwaI