Restriction map for
pFN21AE2716
(Circular) (Six-base) MAPSORT of: pFN21AE2716.seq Check: 6445 from: 1 to: 5410
pFN21AE2716 5410 bp
With 182 enzymes: SgfI *
January 30, 2018 12:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2680 2680
Size: 5410
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3220 278
Size: 53 83 186 936 1684 2468
Fragments arranged by size:
2468 1684 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2753 1937
Size: 816 4594
Acc65I G'GTAC_C
Cuts at: 2731 2731
Size: 5410
AclI AA'CG_TT
Cuts at: 1563 3537 3910 1563
Size: 1974 373 3063
Fragments arranged by size:
3063 1974 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3473 4857 5125 1632
Size: 1841 1384 268 1917
Fragments arranged by size:
1917 1841 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5213
AflIII A'CryG_T
Cuts at: 1203 4309 5286 1203
Size: 3106 977 1327
Fragments arranged by size:
3106 1327 977
AgeI A'CCGG_T
Cuts at: 4241 4241
Size: 5410
AhdI GACnn_n'nnGTC
Cuts at: 2626 4139 2626
Size: 1513 3897
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5410
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5410
AlwNI CAG_nnn'CTG
Cuts at: 2394 3153 4725 2394
Size: 759 1572 3079
Fragments arranged by size:
3079 1572 759
ApaLI G'TGCA_C
Cuts at: 3467 4623 3467
Size: 1156 4254
ApoI r'AATT_y
Cuts at: 1493 2464 2719 2945 3097 5206 1493
Size: 971 255 226 152 2109 1697
Fragments arranged by size:
2109 1697 971 255 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3964 160
Size: 872 2932 1606
Fragments arranged by size:
2932 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5410
AvaI C'yCGr_G
Cuts at: 1870 1943 2735 1870
Size: 73 792 4545
Fragments arranged by size:
4545 792 73
BamHI G'GATC_C
Cuts at: 2740 3107 2740
Size: 367 5043
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2731 4186 618
Size: 325 217 627 944 1455 1842
Fragments arranged by size:
1842 1455 944 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2729 1391
Size: 1338 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2337 2633 928
Size: 971 438 296 3705
Fragments arranged by size:
3705 971 438 296
BbvCI CC'TCA_GC
Cuts at: 2414 2414
Size: 5410
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3601 3635 1903
Size: 34 50 34 1580 34 3678
Fragments arranged by size:
3678 1580 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2236 3302 4512 2236
Size: 1066 1210 3134
Fragments arranged by size:
3134 1210 1066
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5410
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2391 4021 136
Size: 107 122 71 631 605 719 1630 1525
Fragments arranged by size:
1630 1525 719 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2336 5405 2336
Size: 3069 2341
BlpI GC'TnA_GC
Cuts at: 2827 2827
Size: 5410
Bme1580I G_kGCm'C
Cuts at: 1790 3471 4627 1790
Size: 1681 1156 2573
Fragments arranged by size:
2573 1681 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5410
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3141 4099 442
Size: 416 53 83 399 24 1724 958 1753
Fragments arranged by size:
1753 1724 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5410
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4070 1239
Size: 2831 2579
Bpu10I CC'TnA_GC
Cuts at: 2414 2414
Size: 5410
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3535 4400 4698 3535
Size: 865 298 4247
Fragments arranged by size:
4247 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4073 882
Size: 617 2574 2219
Fragments arranged by size:
2574 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3201 493
Size: 711 1997 2702
Fragments arranged by size:
2702 1997 711
BsaBI GATnn'nnATC
Cuts at: 5404 5404
Size: 5410
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3217 3599 4234 275
Size: 53 83 186 936 1684 382 635 1451
Fragments arranged by size:
1684 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3843 4241 4515 4662 5073 3843
Size: 398 274 147 411 4180
Fragments arranged by size:
4180 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5185
Fragments arranged by size:
5185 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5410
BseYI C'CCAG_C
Cuts at: 4613 5109 4613
Size: 496 4914
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4837
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2860 3621 4649 1540
Size: 275 186 859 761 1028 2301
Fragments arranged by size:
2301 1028 859 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2308 3471 3556 4627 2308
Size: 1163 85 1071 3091
Fragments arranged by size:
3091 1163 1071 85
BsmI GAATG_Cn'
Cuts at: 2696 2921 3014 2696
Size: 225 93 5092
Fragments arranged by size:
5092 225 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2308 2729 3471 3556 4627 1391
Size: 399 518 421 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 518 421 399 85
BspEI T'CCGG_A
Cuts at: 5073 5073
Size: 5410
BspHI T'CATG_A
Cuts at: 3299 5029 3299
Size: 1730 3680
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2680 2765 844
Size: 1836 85 3489
Fragments arranged by size:
3489 1836 85
BsrBI CCG'CTC
Cuts at: 1394 1823 3297 1394
Size: 429 1474 3507
Fragments arranged by size:
3507 1474 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2525 3905 4079 65
Size: 1183 1277 1380 174 1396
Fragments arranged by size:
1396 1380 1277 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4054 4241 1700
Size: 110 2244 187 2869
Fragments arranged by size:
2869 2244 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5410
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5410
BssSI C'ACGA_G
Cuts at: 3470 4482 3470
Size: 1012 4398
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5410
BstBI TT'CG_AA
Cuts at: 4214 4214
Size: 5410
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5410
BstYI r'GATC_y
Cuts at: 1972 2336 2740 3107 3492 3509 4961 5047 5059
Size: 364 404 367 385 17 1452 86 12
Cuts at: 5059 5405 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 404 385 367 364 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2180 2229 2356 513
Size: 552 1115 49 127 3567
Fragments arranged by size:
3567 1115 552 127 49
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4671
Fragments arranged by size:
4671 683 56
BtsI GCAGTG_nn'
Cuts at: 2926 3720 3740 2926
Size: 794 20 4596
Fragments arranged by size:
4596 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5410
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5375
DraI TTT'AAA
Cuts at: 2714 2714
Size: 5410
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4417 809
Size: 507 3101 1802
Fragments arranged by size:
3101 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2183 2421 2857
Size: 54 1425 50 275 18 353 238 436
Cuts at: 2857 3746 5375 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 436 353 275 238 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2857 1537
Size: 275 1045 4090
Fragments arranged by size:
4090 1045 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2290 3340 1353
Size: 937 1050 3423
Fragments arranged by size:
3423 1050 937
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3997 4371 4517 387
Size: 1064 138 2408 374 146 1280
Fragments arranged by size:
2408 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3473 4070 4857 5125 1239
Size: 393 1841 597 787 268 1524
Fragments arranged by size:
1841 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2203 2203
Size: 5410
EcoRI G'AATT_C
Cuts at: 2719 3097 2719
Size: 378 5032
FspI TGC'GCA
Cuts at: 3916 3916
Size: 5410
HaeII r_GCGC'y
Cuts at: 1678 4557 1678
Size: 2879 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2058 2090 2612 2644 4051
Size: 32 135 32 486 32 522 32 1407
Cuts at: 4051 4083 4125 4157 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1407 522 486 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2754 3000 669
Size: 988 281 816 246 3079
Fragments arranged by size:
3079 988 816 281 246
HindIII A'AGCT_T
Cuts at: 748 2445 748
Size: 1697 3713
HpaI GTT'AAC
Cuts at: 3000 3000
Size: 5410
KpnI G_GTAC'C
Cuts at: 2735 2735
Size: 5410
MfeI C'AATT_G
Cuts at: 3009 3009
Size: 5410
MluI A'CGCG_T
Cuts at: 5286 5286
Size: 5410
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4524 4708 1305
Size: 3219 184 2007
Fragments arranged by size:
3219 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2185 2423 10
Size: 54 1425 343 353 238 2997
Fragments arranged by size:
2997 1425 353 343 238 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3368 3727 3886 518
Size: 648 2202 359 159 2042
Fragments arranged by size:
2202 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2554 2826 3503 4651 4896 1606
Size: 948 272 677 1148 245 2120
Fragments arranged by size:
2120 1148 948 677 272 245
NcoI C'CATG_G
Cuts at: 513 1065 2180 2229 513
Size: 552 1115 49 3694
Fragments arranged by size:
3694 1115 552 49
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5410
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5410
NotI GC'GGCC_GC
Cuts at: 2857 2857
Size: 5410
NspI r_CATG'y
Cuts at: 1132 1229 2768 4313 1132
Size: 97 1539 1545 2229
Fragments arranged by size:
2229 1545 1539 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5410
PciI A'CATG_T
Cuts at: 4309 4309
Size: 5410
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5410
PmeI GTTT'AAAC
Cuts at: 2714 2714
Size: 5410
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3479 3511 4992 5024 3479
Size: 32 1481 32 3865
Fragments arranged by size:
3865 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2203 2203
Size: 5410
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5410
PsiI TTA'TAA
Cuts at: 2980 2980
Size: 5410
PstI C_TGCA'G
Cuts at: 830 2762 830
Size: 1932 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5410
PvuII CAG'CTG
Cuts at: 2554 2554
Size: 5410
SalI G'TCGA_C
Cuts at: 1936 2752 1936
Size: 816 4594
SapI GCTCTTCn'nnn_
Cuts at: 2290 2290
Size: 5410
SbfI CC_TGCA'GG
Cuts at: 2762 2762
Size: 5410
ScaI AGT'ACT
Cuts at: 3658 5370 3658
Size: 1712 3698
SfcI C'TryA_G
Cuts at: 826 1045 2758 3893 4574 4765 826
Size: 219 1713 1135 681 191 1471
Fragments arranged by size:
1713 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5410
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5410
SmaI CCC'GGG
Cuts at: 1872 2737 1872
Size: 865 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3514 4415 4677 820
Size: 197 926 1571 901 262 1553
Fragments arranged by size:
1571 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3201 493
Size: 2708 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5410
SphI G_CATG'C
Cuts at: 1132 2768 1132
Size: 1636 3774
SspI AAT'ATT
Cuts at: 5 52 3334 5
Size: 47 3282 2081
Fragments arranged by size:
3282 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5410
StyI C'CwwG_G
Cuts at: 513 1065 2180 2229 2849 513
Size: 552 1115 49 620 3074
Fragments arranged by size:
3074 1115 620 552 49
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2369 3437 3454 3607 3792 4237 325
Size: 1255 789 1068 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1068 789 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2056 3656 5368
Size: 274 80 33 51 1443 79 1600 1712
Cuts at: 5368 96
Size: 138
Fragments arranged by size:
1712 1600 1443 274 138 80 79 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5370 5402 5370
Size: 32 5378
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5410
XbaI T'CTAG_A
Cuts at: 2746 2746
Size: 5410
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2193 2193
Size: 5410
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5410
XmaI C'CCGG_G
Cuts at: 1870 2735 1870
Size: 865 4545
XmnI GAAnn'nnTTC
Cuts at: 3539 3539
Size: 5410
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3218 276
Size: 53 83 186 936 1684 2468
Fragments arranged by size:
2468 1684 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI
SrfI SwaI