Restriction map for
pFN21AE2715
(Circular) (Six-base) MAPSORT of: pFN21AE2715.seq Check: 5994 from: 1 to: 6196
pFN21AE2715 6196 bp
With 182 enzymes: SgfI *
February 13, 2018 16:03 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4006 278
Size: 53 83 186 936 2470 2468
Fragments arranged by size:
2470 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2009 3052 3539 1937
Size: 72 1043 487 4594
Fragments arranged by size:
4594 1043 487 72
Acc65I G'GTAC_C
Cuts at: 3517 3517
Size: 6196
AclI AA'CG_TT
Cuts at: 1563 4323 4696 1563
Size: 2760 373 3063
Fragments arranged by size:
3063 2760 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4259 5643 5911 1632
Size: 2627 1384 268 1917
Fragments arranged by size:
2627 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5999
AflIII A'CryG_T
Cuts at: 1203 5095 6072 1203
Size: 3892 977 1327
Fragments arranged by size:
3892 1327 977
AgeI A'CCGG_T
Cuts at: 5027 5027
Size: 6196
AhdI GACnn_n'nnGTC
Cuts at: 2021 4925 2021
Size: 2904 3292
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6196
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3324 1184
Size: 2140 4056
AlwNI CAG_nnn'CTG
Cuts at: 3228 3407 3939 5511 3228
Size: 179 532 1572 3913
Fragments arranged by size:
3913 1572 532 179
ApaLI G'TGCA_C
Cuts at: 4253 5409 4253
Size: 1156 5040
ApoI r'AATT_y
Cuts at: 1493 2288 3505 3731 3883 5992 1493
Size: 795 1217 226 152 2109 1697
Fragments arranged by size:
2109 1697 1217 795 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4750 160
Size: 872 3718 1606
Fragments arranged by size:
3718 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6196
AvaI C'yCGr_G
Cuts at: 1870 1943 3521 1870
Size: 73 1578 4545
Fragments arranged by size:
4545 1578 73
BamHI G'GATC_C
Cuts at: 2997 3526 3893 2997
Size: 529 367 5300
Fragments arranged by size:
5300 529 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3517 4972 618
Size: 325 217 627 1730 1455 1842
Fragments arranged by size:
1842 1730 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2581 3244 3515 1391
Size: 1190 663 271 4072
Fragments arranged by size:
4072 1190 663 271
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2689 3201 3382 928
Size: 971 790 512 181 3742
Fragments arranged by size:
3742 971 790 512 181
BbvCI CC'TCA_GC
Cuts at: 2345 2345
Size: 6196
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4387 4421 1903
Size: 34 2450 34 3678
Fragments arranged by size:
3678 2450 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4088 5298 4088
Size: 1210 4986
BclI T'GATC_A
Cuts at: 1548 2868 1548
Size: 1320 4876
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4807 136
Size: 107 122 71 631 605 3135 1525
Fragments arranged by size:
3135 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6191 6191
Size: 6196
BlpI GC'TnA_GC
Cuts at: 2823 3613 2823
Size: 790 5406
Bme1580I G_kGCm'C
Cuts at: 1790 4257 5413 1790
Size: 2467 1156 2573
Fragments arranged by size:
2573 2467 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6196
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2379 3475 3927
Size: 416 53 83 399 24 962 1096 452
Cuts at: 3927 4885 442
Size: 958 1753
Fragments arranged by size:
1753 1096 962 958 452 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6196
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2101 2101
Size: 6196
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2071 2338 3058 3342 4856 1239
Size: 832 267 720 284 1514 2579
Fragments arranged by size:
2579 1514 832 720 284 267
Bpu10I CC'TnA_GC
Cuts at: 2345 2345
Size: 6196
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2058 2194 4321 5186 5484 2058
Size: 136 2127 865 298 2770
Fragments arranged by size:
2770 2127 865 298 136
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2669 2853 4859 882
Size: 617 1170 184 2006 2219
Fragments arranged by size:
2219 2006 1170 617 184
BsaAI yAC'GTr
Cuts at: 493 1204 3987 493
Size: 711 2783 2702
Fragments arranged by size:
2783 2702 711
BsaBI GATnn'nnATC
Cuts at: 6190 6190
Size: 6196
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4003 4385 5020 275
Size: 53 83 186 936 2470 382 635 1451
Fragments arranged by size:
2470 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2012 2511 4629 5027 5301 5448 5859 2012
Size: 499 2118 398 274 147 411 2349
Fragments arranged by size:
2349 2118 499 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3231 3261 1178
Size: 30 165 30 1828 30 4113
Fragments arranged by size:
4113 1828 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2657 3258 1186
Size: 1471 601 4124
Fragments arranged by size:
4124 1471 601
BseYI C'CCAG_C
Cuts at: 2188 2632 5399 5895 2188
Size: 444 2767 496 2489
Fragments arranged by size:
2767 2489 496 444
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3479 1163
Size: 573 1743 3880
Fragments arranged by size:
3880 1743 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3646 4407 5435 1540
Size: 275 186 1645 761 1028 2301
Fragments arranged by size:
2301 1645 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4257 4342 5413 4257
Size: 85 1071 5040
Fragments arranged by size:
5040 1071 85
BsmI GAATG_Cn'
Cuts at: 2087 3707 3800 2087
Size: 1620 93 4483
Fragments arranged by size:
4483 1620 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2581 3244 3515 4257 4342 5413 1391
Size: 399 791 663 271 742 85 1071 2174
Fragments arranged by size:
2174 1071 791 742 663 399 271 85
BspEI T'CCGG_A
Cuts at: 2511 5859 2511
Size: 3348 2848
BspHI T'CATG_A
Cuts at: 4085 5815 4085
Size: 1730 4466
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3551 844
Size: 2707 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4083 1394
Size: 429 2260 3507
Fragments arranged by size:
3507 2260 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2178 2304 2610 4691 4865 65
Size: 1183 930 126 306 2081 174 1396
Fragments arranged by size:
2081 1396 1183 930 306 174 126
BsrFI r'CCGG_y
Cuts at: 1700 1810 4840 5027 1700
Size: 110 3030 187 2869
Fragments arranged by size:
3030 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2518 96
Size: 2422 3774
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6196
BssSI C'ACGA_G
Cuts at: 3149 4256 5268 3149
Size: 1107 1012 4077
Fragments arranged by size:
4077 1107 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2483 794
Size: 1689 4507
BstBI TT'CG_AA
Cuts at: 5000 5000
Size: 6196
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6196
BstXI CCAn_nnnn'nTGG
Cuts at: 2422 2422
Size: 6196
BstYI r'GATC_y
Cuts at: 1972 2997 3170 3526 3893 4278 4295 5747 5833
Size: 1025 173 356 367 385 17 1452 86
Cuts at: 5833 5845 6191 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1025 385 367 356 346 173
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5644
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5457
Fragments arranged by size:
5457 683 56
BtsI GCAGTG_nn'
Cuts at: 3712 4506 4526 3712
Size: 794 20 5382
Fragments arranged by size:
5382 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6196
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6161
DraI TTT'AAA
Cuts at: 2064 2293 3500 2064
Size: 229 1207 4760
Fragments arranged by size:
4760 1207 229
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5203 809
Size: 507 3887 1802
Fragments arranged by size:
3887 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3445 3643 4532
Size: 54 1425 50 275 18 1615 198 889
Cuts at: 4532 6161 8
Size: 1629 43
Fragments arranged by size:
1629 1615 1425 889 275 198 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3643 1537
Size: 275 1831 4090
Fragments arranged by size:
4090 1831 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2468 2792 2856 3116 3137 4126 1353
Size: 1115 324 64 260 21 989 3423
Fragments arranged by size:
3423 1115 989 324 260 64 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4783 5157 5303 387
Size: 1064 138 3194 374 146 1280
Fragments arranged by size:
3194 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2071 2338 3058 3342 4259 4856 5643
Size: 393 439 267 720 284 917 597 787
Cuts at: 5643 5911 1239
Size: 268 1524
Fragments arranged by size:
1524 917 787 720 597 439 393 284
268 267
EcoO109I rG'GnC_Cy
Cuts at: 2637 2637
Size: 6196
EcoRI G'AATT_C
Cuts at: 3505 3883 3505
Size: 378 5818
FspI TGC'GCA
Cuts at: 4702 4702
Size: 6196
HaeII r_GCGC'y
Cuts at: 1678 5343 1678
Size: 3665 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2007 2039 3339 3371 4837
Size: 32 135 32 435 32 1300 32 1466
Cuts at: 4837 4869 4911 4943 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1466 1300 435 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2600 3250 3412 3540 3786 669
Size: 988 281 662 650 162 128 246 3079
Fragments arranged by size:
3079 988 662 650 281 246 162 128
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6196
HpaI GTT'AAC
Cuts at: 3250 3412 3786 3250
Size: 162 374 5660
Fragments arranged by size:
5660 374 162
KpnI G_GTAC'C
Cuts at: 3521 3521
Size: 6196
MfeI C'AATT_G
Cuts at: 3795 3795
Size: 6196
MluI A'CGCG_T
Cuts at: 6072 6072
Size: 6196
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2134 3004 3359 5310 5494 1305
Size: 829 870 355 1951 184 2007
Fragments arranged by size:
2007 1951 870 829 355 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4374
Fragments arranged by size:
4374 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3188 4154 4513 4672 518
Size: 648 2022 966 359 159 2042
Fragments arranged by size:
2042 2022 966 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3404 3612 4289 5437 5682 1606
Size: 1798 208 677 1148 245 2120
Fragments arranged by size:
2120 1798 1148 677 245 208
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5644
NdeI CA'TA_TG
Cuts at: 387 2311 387
Size: 1924 4272
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6196
NotI GC'GGCC_GC
Cuts at: 3643 3643
Size: 6196
NspI r_CATG'y
Cuts at: 1132 1229 3554 5099 1132
Size: 97 2325 1545 2229
Fragments arranged by size:
2325 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2260 3162 1348
Size: 912 902 4382
Fragments arranged by size:
4382 912 902
PciI A'CATG_T
Cuts at: 5095 5095
Size: 6196
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2539 2685 1108
Size: 1431 146 4619
Fragments arranged by size:
4619 1431 146
PfoI T'CCnGG_A
Cuts at: 2087 2087
Size: 6196
PmeI GTTT'AAAC
Cuts at: 3500 3500
Size: 6196
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4265 4297 5778 5810 4265
Size: 32 1481 32 4651
Fragments arranged by size:
4651 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2637 2637
Size: 6196
PshAI GACnn'nnGTC
Cuts at: 1613 2319 1613
Size: 706 5490
PsiI TTA'TAA
Cuts at: 3766 3766
Size: 6196
PstI C_TGCA'G
Cuts at: 830 3548 830
Size: 2718 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6196
PvuII CAG'CTG
Cuts at: 3404 3404
Size: 6196
SalI G'TCGA_C
Cuts at: 1936 3538 1936
Size: 1602 4594
SbfI CC_TGCA'GG
Cuts at: 3548 3548
Size: 6196
ScaI AGT'ACT
Cuts at: 4444 6156 4444
Size: 1712 4484
SfcI C'TryA_G
Cuts at: 826 1045 2031 3544 4679 5360 5551 826
Size: 219 986 1513 1135 681 191 1471
Fragments arranged by size:
1513 1471 1135 986 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6196
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6196
SmaI CCC'GGG
Cuts at: 1872 3523 1872
Size: 1651 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2073 2173 4300 5201 5463 820
Size: 197 926 130 100 2127 901 262 1553
Fragments arranged by size:
2127 1553 926 901 262 197 130 100
SnaBI TAC'GTA
Cuts at: 493 3987 493
Size: 3494 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6196
SphI G_CATG'C
Cuts at: 1132 3554 1132
Size: 2422 3774
SspI AAT'ATT
Cuts at: 5 52 2406 4120 5
Size: 47 2354 1714 2081
Fragments arranged by size:
2354 2081 1714 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6196
StyI C'CwwG_G
Cuts at: 513 1065 3043 3635 513
Size: 552 1978 592 3074
Fragments arranged by size:
3074 1978 592 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2174 3503 4223 4240 4393 4578 5023
Size: 1255 594 1329 720 17 153 185 445
Cuts at: 5023 325
Size: 1498
Fragments arranged by size:
1498 1329 1255 720 594 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2518 4442 6154
Size: 274 80 33 51 1443 541 1924 1712
Cuts at: 6154 96
Size: 138
Fragments arranged by size:
1924 1712 1443 541 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6156 6188 6156
Size: 32 6164
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6196
XbaI T'CTAG_A
Cuts at: 3532 3532
Size: 6196
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2191 2392 3196 2191
Size: 201 804 5191
Fragments arranged by size:
5191 804 201
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6196
XmaI C'CCGG_G
Cuts at: 1870 3521 1870
Size: 1651 4545
XmnI GAAnn'nnTTC
Cuts at: 3110 4325 3110
Size: 1215 4981
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4004 276
Size: 53 83 186 936 2470 2468
Fragments arranged by size:
2470 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI