Restriction map for
pFN21AE2707
(Circular) (Six-base) MAPSORT of: pFN21AE2707.seq Check: 5708 from: 1 to: 5416
pFN21AE2707 5416 bp
With 182 enzymes: SgfI *
December 15, 2017 10:10 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3226 278
Size: 53 83 186 936 1690 2468
Fragments arranged by size:
2468 1690 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2759 1937
Size: 822 4594
Acc65I G'GTAC_C
Cuts at: 2737 2737
Size: 5416
AclI AA'CG_TT
Cuts at: 1563 3543 3916 1563
Size: 1980 373 3063
Fragments arranged by size:
3063 1980 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2286 2513 2626 3479 4863 5131 1632
Size: 654 227 113 853 1384 268 1917
Fragments arranged by size:
1917 1384 853 654 268 227 113
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5219
AflIII A'CryG_T
Cuts at: 1203 4315 5292 1203
Size: 3112 977 1327
Fragments arranged by size:
3112 1327 977
AgeI A'CCGG_T
Cuts at: 4247 4247
Size: 5416
AhdI GACnn_n'nnGTC
Cuts at: 2194 4145 2194
Size: 1951 3465
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5416
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5416
AlwNI CAG_nnn'CTG
Cuts at: 2026 3159 4731 2026
Size: 1133 1572 2711
Fragments arranged by size:
2711 1572 1133
ApaLI G'TGCA_C
Cuts at: 3473 4629 3473
Size: 1156 4260
ApoI r'AATT_y
Cuts at: 1493 2054 2725 2951 3103 5212 1493
Size: 561 671 226 152 2109 1697
Fragments arranged by size:
2109 1697 671 561 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2345 3970 160
Size: 872 1313 1625 1606
Fragments arranged by size:
1625 1606 1313 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5416
AvaI C'yCGr_G
Cuts at: 1870 1943 2741 1870
Size: 73 798 4545
Fragments arranged by size:
4545 798 73
BamHI G'GATC_C
Cuts at: 2746 3113 2746
Size: 367 5049
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2565 2737 4192 618
Size: 325 217 627 778 172 1455 1842
Fragments arranged by size:
1842 1455 778 627 325 217 172
BanII G_rGCy'C
Cuts at: 1391 2735 1391
Size: 1344 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2144 928
Size: 971 245 4200
Fragments arranged by size:
4200 971 245
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3607 3641 1903
Size: 34 1670 34 3678
Fragments arranged by size:
3678 1670 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3308 4518 3308
Size: 1210 4206
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5416
BfrBI ATG'CAT
Cuts at: 2438 2438
Size: 5416
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4027 136
Size: 107 122 71 631 605 2355 1525
Fragments arranged by size:
2355 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5411 5411
Size: 5416
BlpI GC'TnA_GC
Cuts at: 2833 2833
Size: 5416
Bme1580I G_kGCm'C
Cuts at: 1790 3477 4633 1790
Size: 1687 1156 2573
Fragments arranged by size:
2573 1687 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5416
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3147 4105 442
Size: 416 53 83 399 24 1730 958 1753
Fragments arranged by size:
1753 1730 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5416
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4076 1239
Size: 2837 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3541 4406 4704 3541
Size: 865 298 4253
Fragments arranged by size:
4253 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4079 882
Size: 617 2580 2219
Fragments arranged by size:
2580 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3207 493
Size: 711 2003 2702
Fragments arranged by size:
2702 2003 711
BsaBI GATnn'nnATC
Cuts at: 5410 5410
Size: 5416
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3223 3605 4240 275
Size: 53 83 186 936 1690 382 635 1451
Fragments arranged by size:
1690 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3849 4247 4521 4668 5079 3849
Size: 398 274 147 411 4186
Fragments arranged by size:
4186 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5191
Fragments arranged by size:
5191 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5416
BseYI C'CCAG_C
Cuts at: 4619 5115 4619
Size: 496 4920
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4843
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2866 3627 4655 1540
Size: 275 186 865 761 1028 2301
Fragments arranged by size:
2301 1028 865 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3477 3562 4633 3477
Size: 85 1071 4260
Fragments arranged by size:
4260 1071 85
BsmI GAATG_Cn'
Cuts at: 2440 2927 3020 2440
Size: 487 93 4836
Fragments arranged by size:
4836 487 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2735 3477 3562 4633 1391
Size: 399 945 742 85 1071 2174
Fragments arranged by size:
2174 1071 945 742 399 85
BspEI T'CCGG_A
Cuts at: 5079 5079
Size: 5416
BspHI T'CATG_A
Cuts at: 3305 5035 3305
Size: 1730 3686
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2771 844
Size: 1927 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3303 1394
Size: 429 1480 3507
Fragments arranged by size:
3507 1480 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3911 4085 65
Size: 1183 2663 174 1396
Fragments arranged by size:
2663 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2154 4060 4247 1700
Size: 110 344 1906 187 2869
Fragments arranged by size:
2869 1906 344 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5416
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5416
BssSI C'ACGA_G
Cuts at: 2015 3476 4488 2015
Size: 1461 1012 2943
Fragments arranged by size:
2943 1461 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5416
BstBI TT'CG_AA
Cuts at: 4220 4220
Size: 5416
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5416
BstYI r'GATC_y
Cuts at: 1972 2022 2746 3113 3498 3515 4967 5053 5065
Size: 50 724 367 385 17 1452 86 12
Cuts at: 5065 5411 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 724 385 367 346 86 50
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3925
Fragments arranged by size:
3925 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4677
Fragments arranged by size:
4677 683 56
BtsI GCAGTG_nn'
Cuts at: 2932 3726 3746 2932
Size: 794 20 4602
Fragments arranged by size:
4602 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5416
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5381
DraI TTT'AAA
Cuts at: 2720 2720
Size: 5416
DraIII CAC_nnn'GTG
Cuts at: 2474 2474
Size: 5416
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4423 809
Size: 507 3107 1802
Fragments arranged by size:
3107 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2863 3752 5381
Size: 54 1425 50 275 18 1033 889 1629
Cuts at: 5381 8
Size: 43
Fragments arranged by size:
1629 1425 1033 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2863 1537
Size: 275 1051 4090
Fragments arranged by size:
4090 1051 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2508 2670 3346 1353
Size: 1155 162 676 3423
Fragments arranged by size:
3423 1155 676 162
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2028 4003 4377 4523 387
Size: 1064 138 439 1975 374 146 1280
Fragments arranged by size:
1975 1280 1064 439 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2286 2513 2626 3479 4076 4863 5131
Size: 393 654 227 113 853 597 787 268
Cuts at: 5131 1239
Size: 1524
Fragments arranged by size:
1524 853 787 654 597 393 268 227
113
EcoO109I rG'GnC_Cy
Cuts at: 2063 2063
Size: 5416
EcoRI G'AATT_C
Cuts at: 2725 3103 2725
Size: 378 5038
FspI TGC'GCA
Cuts at: 3922 3922
Size: 5416
HaeII r_GCGC'y
Cuts at: 1678 4563 1678
Size: 2885 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2180 2212 2637 2669 4057
Size: 32 135 32 608 32 425 32 1388
Cuts at: 4057 4089 4131 4163 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1388 608 425 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2199 2760 3006 669
Size: 988 281 261 561 246 3079
Fragments arranged by size:
3079 988 561 281 261 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5416
HpaI GTT'AAC
Cuts at: 3006 3006
Size: 5416
KpnI G_GTAC'C
Cuts at: 2741 2741
Size: 5416
MfeI C'AATT_G
Cuts at: 3015 3015
Size: 5416
MluI A'CGCG_T
Cuts at: 5292 5292
Size: 5416
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4530 4714 1305
Size: 3225 184 2007
Fragments arranged by size:
3225 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3594
Fragments arranged by size:
3594 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3374 3733 3892 518
Size: 648 2208 359 159 2042
Fragments arranged by size:
2208 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2832 3509 4657 4902 1606
Size: 1226 677 1148 245 2120
Fragments arranged by size:
2120 1226 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3925
Fragments arranged by size:
3925 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5416
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5416
NotI GC'GGCC_GC
Cuts at: 2863 2863
Size: 5416
NsiI A_TGCA'T
Cuts at: 2440 2440
Size: 5416
NspI r_CATG'y
Cuts at: 1132 1229 2774 4319 1132
Size: 97 1545 1545 2229
Fragments arranged by size:
2229 1545 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2192 1348
Size: 844 4572
PciI A'CATG_T
Cuts at: 4315 4315
Size: 5416
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5416
PmeI GTTT'AAAC
Cuts at: 2720 2720
Size: 5416
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3485 3517 4998 5030 3485
Size: 32 1481 32 3871
Fragments arranged by size:
3871 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5416
PsiI TTA'TAA
Cuts at: 2986 2986
Size: 5416
PstI C_TGCA'G
Cuts at: 830 2768 830
Size: 1938 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5416
SalI G'TCGA_C
Cuts at: 1936 2758 1936
Size: 822 4594
SbfI CC_TGCA'GG
Cuts at: 2768 2768
Size: 5416
ScaI AGT'ACT
Cuts at: 3664 5376 3664
Size: 1712 3704
SfcI C'TryA_G
Cuts at: 826 1045 2142 2222 2764 3899 4580 4771 826
Size: 219 1097 80 542 1135 681 191 1471
Fragments arranged by size:
1471 1135 1097 681 542 219 191 80
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5416
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5416
SmaI CCC'GGG
Cuts at: 1872 2743 1872
Size: 871 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3520 4421 4683 820
Size: 197 926 1577 901 262 1553
Fragments arranged by size:
1577 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3207 493
Size: 2714 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5416
SphI G_CATG'C
Cuts at: 1132 2774 1132
Size: 1642 3774
SspI AAT'ATT
Cuts at: 5 52 3340 5
Size: 47 3288 2081
Fragments arranged by size:
3288 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5416
StyI C'CwwG_G
Cuts at: 513 1065 2004 2855 513
Size: 552 939 851 3074
Fragments arranged by size:
3074 939 851 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3443 3460 3613 3798 4243 325
Size: 1255 1863 17 153 185 445 1498
Fragments arranged by size:
1863 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3662 5374 96
Size: 274 80 33 51 1443 1685 1712 138
Fragments arranged by size:
1712 1685 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2303 2335 5376 5408 2303
Size: 32 3041 32 2311
Fragments arranged by size:
3041 2311 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5416
XbaI T'CTAG_A
Cuts at: 2752 2752
Size: 5416
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5416
XmaI C'CCGG_G
Cuts at: 1870 2741 1870
Size: 871 4545
XmnI GAAnn'nnTTC
Cuts at: 3545 3545
Size: 5416
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3224 276
Size: 53 83 186 936 1690 2468
Fragments arranged by size:
2468 1690 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI