Restriction map for
pFN21AE2702
(Circular) (Six-base) MAPSORT of: pFN21AE2702.seq Check: 1583 from: 1 to: 5992
pFN21AE2702 5992 bp
With 182 enzymes: SgfI *
September 11, 2017 14:08 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3802 278
Size: 53 83 186 936 2266 2468
Fragments arranged by size:
2468 2266 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3335 1937
Size: 1398 4594
Acc65I G'GTAC_C
Cuts at: 3313 3313
Size: 5992
AclI AA'CG_TT
Cuts at: 1563 4119 4492 1563
Size: 2556 373 3063
Fragments arranged by size:
3063 2556 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4055 5439 5707 1632
Size: 2423 1384 268 1917
Fragments arranged by size:
2423 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5795
AflIII A'CryG_T
Cuts at: 1203 4891 5868 1203
Size: 3688 977 1327
Fragments arranged by size:
3688 1327 977
AgeI A'CCGG_T
Cuts at: 4823 4823
Size: 5992
AhdI GACnn_n'nnGTC
Cuts at: 4721 4721
Size: 5992
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5992
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5992
AlwNI CAG_nnn'CTG
Cuts at: 2085 3289 3735 5307 2085
Size: 1204 446 1572 2770
Fragments arranged by size:
2770 1572 1204 446
ApaLI G'TGCA_C
Cuts at: 4049 5205 4049
Size: 1156 4836
ApoI r'AATT_y
Cuts at: 1493 3301 3527 3679 5788 1493
Size: 1808 226 152 2109 1697
Fragments arranged by size:
2109 1808 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2237 4546 160
Size: 872 1205 2309 1606
Fragments arranged by size:
2309 1606 1205 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5992
AvaI C'yCGr_G
Cuts at: 1870 1943 3317 1870
Size: 73 1374 4545
Fragments arranged by size:
4545 1374 73
BamHI G'GATC_C
Cuts at: 2408 3322 3689 2408
Size: 914 367 4711
Fragments arranged by size:
4711 914 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2344 3313 4768 618
Size: 325 217 627 557 969 1455 1842
Fragments arranged by size:
1842 1455 969 627 557 325 217
BanII G_rGCy'C
Cuts at: 1391 3311 1391
Size: 1920 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5021
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4183 4217 1903
Size: 34 50 34 2162 34 3678
Fragments arranged by size:
3678 2162 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2604 3884 5094 2604
Size: 1280 1210 3502
Fragments arranged by size:
3502 1280 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5992
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4603 136
Size: 107 122 71 631 605 2931 1525
Fragments arranged by size:
2931 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5987 5987
Size: 5992
BlpI GC'TnA_GC
Cuts at: 3409 3409
Size: 5992
Bme1580I G_kGCm'C
Cuts at: 1790 4053 5209 1790
Size: 2263 1156 2573
Fragments arranged by size:
2573 2263 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5992
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3723 4681 442
Size: 416 53 83 399 24 2306 958 1753
Fragments arranged by size:
2306 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5992
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2107 2915 4652 1239
Size: 868 808 1737 2579
Fragments arranged by size:
2579 1737 868 808
Bpu10I CC'TnA_GC
Cuts at: 3224 3224
Size: 5992
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2163 2792 3066 4117 4982 5280 2163
Size: 629 274 1051 865 298 2875
Fragments arranged by size:
2875 1051 865 629 298 274
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2304 4655 882
Size: 617 805 2351 2219
Fragments arranged by size:
2351 2219 805 617
BsaAI yAC'GTr
Cuts at: 493 1204 3783 493
Size: 711 2579 2702
Fragments arranged by size:
2702 2579 711
BsaBI GATnn'nnATC
Cuts at: 5986 5986
Size: 5992
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2052 3799 4181 4816
Size: 53 83 186 936 519 1747 382 635
Cuts at: 4816 275
Size: 1451
Fragments arranged by size:
1747 1451 936 635 519 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4425 4823 5097 5244 5655 4425
Size: 398 274 147 411 4762
Fragments arranged by size:
4762 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2080 2110 2594 2624 2700
Size: 30 165 30 677 30 484 30 76
Cuts at: 2700 2730 1178
Size: 30 4440
Fragments arranged by size:
4440 677 484 165 76 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2708 1186
Size: 1522 4470
BseYI C'CCAG_C
Cuts at: 2154 5195 5691 2154
Size: 3041 496 2455
Fragments arranged by size:
3041 2455 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2142 1163
Size: 573 406 5013
Fragments arranged by size:
5013 573 406
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2136 3442 4203 5231 1540
Size: 275 186 135 1306 761 1028 2301
Fragments arranged by size:
2301 1306 1028 761 275 186 135
BsiHKAI G_wGCw'C
Cuts at: 2690 4053 4138 5209 2690
Size: 1363 85 1071 3473
Fragments arranged by size:
3473 1363 1071 85
BsmI GAATG_Cn'
Cuts at: 3178 3503 3596 3178
Size: 325 93 5574
Fragments arranged by size:
5574 325 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2690 3311 4053 4138 5209 1391
Size: 399 900 621 742 85 1071 2174
Fragments arranged by size:
2174 1071 900 742 621 399 85
BspEI T'CCGG_A
Cuts at: 5655 5655
Size: 5992
BspHI T'CATG_A
Cuts at: 2316 3881 5611 2316
Size: 1565 1730 2697
Fragments arranged by size:
2697 1730 1565
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3347 844
Size: 2503 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3235 3879 1394
Size: 429 1412 644 3507
Fragments arranged by size:
3507 1412 644 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4487 4661 65
Size: 1183 3239 174 1396
Fragments arranged by size:
3239 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4636 4823 1700
Size: 110 2826 187 2869
Fragments arranged by size:
2869 2826 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5992
BssHII G'CGCG_C
Cuts at: 1925 2041 1925
Size: 116 5876
BssSI C'ACGA_G
Cuts at: 4052 5064 4052
Size: 1012 4980
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5992
BstBI TT'CG_AA
Cuts at: 4796 4796
Size: 5992
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5992
BstXI CCAn_nnnn'nTGG
Cuts at: 2109 2109
Size: 5992
BstYI r'GATC_y
Cuts at: 1972 2408 3322 3689 4074 4091 5543 5629 5641
Size: 436 914 367 385 17 1452 86 12
Cuts at: 5641 5987 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 914 436 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2853 2853
Size: 5992
BtgI C'CryG_G
Cuts at: 513 1065 2046 2102 513
Size: 552 981 56 4403
Fragments arranged by size:
4403 981 552 56
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2338 487
Size: 683 56 1112 4141
Fragments arranged by size:
4141 1112 683 56
BtsI GCAGTG_nn'
Cuts at: 2813 3508 4302 4322 2813
Size: 695 794 20 4483
Fragments arranged by size:
4483 794 695 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5992
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3082 3117 529
Size: 35 2518 35 3404
Fragments arranged by size:
3404 2518 35 35
DraI TTT'AAA
Cuts at: 3296 3296
Size: 5992
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4999 809
Size: 507 3683 1802
Fragments arranged by size:
3683 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2133 3165 3439
Size: 54 1425 50 275 18 303 1032 274
Cuts at: 3439 4328 5957 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1032 889 303 275 274 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2133 3439 1537
Size: 275 321 1306 4090
Fragments arranged by size:
4090 1306 321 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2189 2303 2541 2771 3115 3922 1353
Size: 836 114 238 230 344 807 3423
Fragments arranged by size:
3423 836 807 344 238 230 114
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2421 4579 4953 5099 387
Size: 1064 138 832 2158 374 146 1280
Fragments arranged by size:
2158 1280 1064 832 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2107 2915 4055 4652 5439 5707 1239
Size: 393 475 808 1140 597 787 268 1524
Fragments arranged by size:
1524 1140 808 787 597 475 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2255 2957 3160 2255
Size: 702 203 5087
Fragments arranged by size:
5087 702 203
EcoRI G'AATT_C
Cuts at: 3301 3679 3301
Size: 378 5614
FspI TGC'GCA
Cuts at: 4498 4498
Size: 5992
HaeII r_GCGC'y
Cuts at: 1678 2868 5139 1678
Size: 1190 2271 2531
Fragments arranged by size:
2531 2271 1190
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2080 2112 2400 2432 2456
Size: 32 135 32 508 32 288 32 24
Cuts at: 2456 2488 3251 3283 4633 4665 4707 4739 1373
Size: 32 763 32 1350 32 42 32 2626
Fragments arranged by size:
2626 1350 763 508 288 135 42 32
32 32 32 32 32 32 32 24
HincII GTy'rAC
Cuts at: 669 1657 1938 2833 3336 3582 669
Size: 988 281 895 503 246 3079
Fragments arranged by size:
3079 988 895 503 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5992
HpaI GTT'AAC
Cuts at: 3582 3582
Size: 5992
KpnI G_GTAC'C
Cuts at: 3317 3317
Size: 5992
MfeI C'AATT_G
Cuts at: 2541 3591 2541
Size: 1050 4942
MluI A'CGCG_T
Cuts at: 5868 5868
Size: 5992
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5106 5290 1305
Size: 3801 184 2007
Fragments arranged by size:
3801 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4170
Fragments arranged by size:
4170 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2107 3950 4309 4468 518
Size: 648 941 1843 359 159 2042
Fragments arranged by size:
2042 1843 941 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2048 2071 2085 2126 2132 2607 3217 3289
Size: 442 23 14 41 6 475 610 72
Cuts at: 3289 3408 4085 5233 5478 1606
Size: 119 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 610 475 442 245 119
72 41 23 14 6
NcoI C'CATG_G
Cuts at: 513 1065 2102 513
Size: 552 1037 4403
Fragments arranged by size:
4403 1037 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5992
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5992
NotI GC'GGCC_GC
Cuts at: 3439 3439
Size: 5992
NspI r_CATG'y
Cuts at: 1132 1229 2162 3350 4895 1132
Size: 97 933 1188 1545 2229
Fragments arranged by size:
2229 1545 1188 933 97
PasI CC'CwG_GG
Cuts at: 1348 2164 1348
Size: 816 5176
PciI A'CATG_T
Cuts at: 4891 4891
Size: 5992
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2085 3289 1108
Size: 977 1204 3811
Fragments arranged by size:
3811 1204 977
PmeI GTTT'AAAC
Cuts at: 3296 3296
Size: 5992
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4061 4093 5574 5606 4061
Size: 32 1481 32 4447
Fragments arranged by size:
4447 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2957 2957
Size: 5992
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5992
PsiI TTA'TAA
Cuts at: 3562 3562
Size: 5992
PstI C_TGCA'G
Cuts at: 830 2131 3257 3344 830
Size: 1301 1126 87 3478
Fragments arranged by size:
3478 1301 1126 87
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5992
PvuII CAG'CTG
Cuts at: 2085 2126 2607 3217 3289 2085
Size: 41 481 610 72 4788
Fragments arranged by size:
4788 610 481 72 41
SacII CC_GC'GG
Cuts at: 2049 2049
Size: 5992
SalI G'TCGA_C
Cuts at: 1936 3334 1936
Size: 1398 4594
SapI GCTCTTCn'nnn_
Cuts at: 3115 3115
Size: 5992
SbfI CC_TGCA'GG
Cuts at: 3344 3344
Size: 5992
ScaI AGT'ACT
Cuts at: 2900 3077 4240 5952 2900
Size: 177 1163 1712 2940
Fragments arranged by size:
2940 1712 1163 177
SfcI C'TryA_G
Cuts at: 826 1045 2127 3253 3340 4475 5156 5347 826
Size: 219 1082 1126 87 1135 681 191 1471
Fragments arranged by size:
1471 1135 1126 1082 681 219 191 87
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5992
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5992
SmaI CCC'GGG
Cuts at: 1872 3319 1872
Size: 1447 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2178 2771 3081 4096 4997 5259
Size: 197 926 235 593 310 1015 901 262
Cuts at: 5259 820
Size: 1553
Fragments arranged by size:
1553 1015 926 901 593 310 262 235
197
SnaBI TAC'GTA
Cuts at: 493 3783 493
Size: 3290 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5992
SphI G_CATG'C
Cuts at: 1132 2162 3350 1132
Size: 1030 1188 3774
Fragments arranged by size:
3774 1188 1030
SspI AAT'ATT
Cuts at: 5 52 2235 3916 5
Size: 47 2183 1681 2081
Fragments arranged by size:
2183 2081 1681 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5992
StyI C'CwwG_G
Cuts at: 513 1065 2102 2931 3093 3431 513
Size: 552 1037 829 162 338 3074
Fragments arranged by size:
3074 1037 829 552 338 162
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2322 4019 4036 4189 4374 4819 325
Size: 1255 742 1697 17 153 185 445 1498
Fragments arranged by size:
1697 1498 1255 742 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2601 2898 3075
Size: 274 80 33 51 1443 624 297 177
Cuts at: 3075 4238 5950 96
Size: 1163 1712 138
Fragments arranged by size:
1712 1443 1163 624 297 274 177 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2104 2136 2578 2610 2700 2732 5952 5984 2104
Size: 32 442 32 90 32 3220 32 2112
Fragments arranged by size:
3220 2112 442 90 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5992
XbaI T'CTAG_A
Cuts at: 3328 3328
Size: 5992
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3286 3286
Size: 5992
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5992
XmaI C'CCGG_G
Cuts at: 1870 3317 1870
Size: 1447 4545
XmnI GAAnn'nnTTC
Cuts at: 4121 4121
Size: 5992
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3800 276
Size: 53 83 186 936 2266 2468
Fragments arranged by size:
2468 2266 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SacII SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI
PsrI RsrII SacI SanDI SexAI SfiI SfoI SrfI
SwaI