Restriction map for
pFN21AE2699
(Circular) (Six-base) MAPSORT of: pFN21AE2699.seq Check: 4799 from: 1 to: 7459
pFN21AE2699 7459 bp
With 182 enzymes: SgfI *
July 6, 2017 14:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5269 278
Size: 53 83 186 936 3733 2468
Fragments arranged by size:
3733 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2560 4802 1937
Size: 623 2242 4594
Fragments arranged by size:
4594 2242 623
Acc65I G'GTAC_C
Cuts at: 3689 4780 3689
Size: 1091 6368
AclI AA'CG_TT
Cuts at: 1563 5586 5959 1563
Size: 4023 373 3063
Fragments arranged by size:
4023 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2982 5522 6906 7174 1632
Size: 1350 2540 1384 268 1917
Fragments arranged by size:
2540 1917 1384 1350 268
AflII C'TTAA_G
Cuts at: 820 1017 2723 820
Size: 197 1706 5556
Fragments arranged by size:
5556 1706 197
AflIII A'CryG_T
Cuts at: 1203 2871 6358 7335 1203
Size: 1668 3487 977 1327
Fragments arranged by size:
3487 1668 1327 977
AgeI A'CCGG_T
Cuts at: 6290 6290
Size: 7459
AhdI GACnn_n'nnGTC
Cuts at: 6188 6188
Size: 7459
AleI CACnn'nnGTG
Cuts at: 1166 3611 1166
Size: 2445 5014
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7459
AlwNI CAG_nnn'CTG
Cuts at: 2714 2734 5202 6774 2714
Size: 20 2468 1572 3399
Fragments arranged by size:
3399 2468 1572 20
ApaI G_GGCC'C
Cuts at: 3523 3523
Size: 7459
ApaLI G'TGCA_C
Cuts at: 5516 6672 5516
Size: 1156 6303
ApoI r'AATT_y
Cuts at: 1493 3268 4571 4768 4994 5146 7255 1493
Size: 1775 1303 197 226 152 2109 1697
Fragments arranged by size:
2109 1775 1697 1303 226 197 152
AseI AT'TA_AT
Cuts at: 160 1032 4709 6013 160
Size: 872 3677 1304 1606
Fragments arranged by size:
3677 1606 1304 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7459
AvaI C'yCGr_G
Cuts at: 1870 1943 2033 2927 4463 4784 1870
Size: 73 90 894 1536 321 4545
Fragments arranged by size:
4545 1536 894 321 90 73
AvrII C'CTAG_G
Cuts at: 3655 3655
Size: 7459
BamHI G'GATC_C
Cuts at: 4221 4789 5156 4221
Size: 568 367 6524
Fragments arranged by size:
6524 568 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3066 3689 4780 6235 618
Size: 325 217 627 1279 623 1091 1455 1842
Fragments arranged by size:
1842 1455 1279 1091 627 623 325 217
BanII G_rGCy'C
Cuts at: 1391 2040 2571 3523 3904 4778 1391
Size: 649 531 952 381 874 4072
Fragments arranged by size:
4072 952 874 649 531 381
BbeI G_GCGC'C
Cuts at: 3070 3070
Size: 7459
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2544 4476 928
Size: 971 645 1932 3911
Fragments arranged by size:
3911 1932 971 645
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2005 2039 2362 2396 5650 5684 1903
Size: 34 68 34 323 34 3254 34 3678
Fragments arranged by size:
3678 3254 323 68 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5351 6561 5351
Size: 1210 6249
BclI T'GATC_A
Cuts at: 1548 3093 1548
Size: 1545 5914
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4013 6070 136
Size: 107 122 71 631 605 2341 2057 1525
Fragments arranged by size:
2341 2057 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7454 7454
Size: 7459
BlpI GC'TnA_GC
Cuts at: 4876 4876
Size: 7459
Bme1580I G_kGCm'C
Cuts at: 1790 2065 3523 4492 5520 6676 1790
Size: 275 1458 969 1028 1156 2573
Fragments arranged by size:
2573 1458 1156 1028 969 275
BmgBI CAC'GTC
Cuts at: 1318 3850 1318
Size: 2532 4927
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3132 5190 6148
Size: 416 53 83 399 24 1715 2058 958
Cuts at: 6148 442
Size: 1753
Fragments arranged by size:
2058 1753 1715 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7459
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2569 3714 6119 1239
Size: 1330 1145 2405 2579
Fragments arranged by size:
2579 2405 1330 1145
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2636 3175 5584 6449 6747 2636
Size: 539 2409 865 298 3348
Fragments arranged by size:
3348 2409 865 539 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6122 882
Size: 617 4623 2219
Fragments arranged by size:
4623 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2872 3613 5250 493
Size: 711 1668 741 1637 2702
Fragments arranged by size:
2702 1668 1637 741 711
BsaBI GATnn'nnATC
Cuts at: 3168 7453 3168
Size: 4285 3174
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3067 5266 5648 6283
Size: 53 83 186 936 1534 2199 382 635
Cuts at: 6283 275
Size: 1451
Fragments arranged by size:
2199 1534 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3675 5892 6290 6564 6711 7122 3675
Size: 2217 398 274 147 411 4012
Fragments arranged by size:
4012 2217 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2278 2308 1178
Size: 30 165 30 875 30 6329
Fragments arranged by size:
6329 875 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2012 2057 2335 3585 4733 1186
Size: 826 45 278 1250 1148 3912
Fragments arranged by size:
3912 1250 1148 826 278 45
BseYI C'CCAG_C
Cuts at: 2028 2058 3021 6662 7158 2028
Size: 30 963 3641 496 2329
Fragments arranged by size:
3641 2329 963 496 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2381 1163
Size: 573 645 6241
Fragments arranged by size:
6241 645 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4909 5670 6698 1540
Size: 275 186 2908 761 1028 2301
Fragments arranged by size:
2908 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2013 2571 3753 3904 4236 5520 5605 6676 2013
Size: 558 1182 151 332 1284 85 1071 2796
Fragments arranged by size:
2796 1284 1182 1071 558 332 151 85
BsmI GAATG_Cn'
Cuts at: 3666 4970 5063 3666
Size: 1304 93 6062
Fragments arranged by size:
6062 1304 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2013 2040 2065 2571 3523 3753 3904
Size: 399 223 27 25 506 952 230 151
Cuts at: 3904 4236 4492 4778 5520 5605 6676 1391
Size: 332 256 286 742 85 1071 2174
Fragments arranged by size:
2174 1071 952 742 506 399 332 286
256 230 223 151 85 27 25
BspEI T'CCGG_A
Cuts at: 7122 7122
Size: 7459
BspHI T'CATG_A
Cuts at: 3648 4173 5348 7078 3648
Size: 525 1175 1730 4029
Fragments arranged by size:
4029 1730 1175 525
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4814 844
Size: 3970 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 5346 1394
Size: 429 3523 3507
Fragments arranged by size:
3523 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2380 3000 4643 5954 6128 65
Size: 1183 1132 620 1643 1311 174 1396
Fragments arranged by size:
1643 1396 1311 1183 1132 620 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6103 6290 1700
Size: 110 4293 187 2869
Fragments arranged by size:
4293 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2988 3387 96
Size: 2892 399 4168
Fragments arranged by size:
4168 2892 399
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7459
BssSI C'ACGA_G
Cuts at: 5519 6531 5519
Size: 1012 6447
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7459
BstBI TT'CG_AA
Cuts at: 4732 6263 4732
Size: 1531 5928
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7459
BstXI CCAn_nnnn'nTGG
Cuts at: 3367 3738 4492 3367
Size: 371 754 6334
Fragments arranged by size:
6334 754 371
BstYI r'GATC_y
Cuts at: 1972 2491 2800 4221 4789 5156 5541 5558 7010
Size: 519 309 1421 568 367 385 17 1452
Cuts at: 7010 7096 7108 7454 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1421 568 519 385 367 346
309 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2898 3062 513
Size: 552 939 894 164 4910
Fragments arranged by size:
4910 939 894 552 164
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6720
Fragments arranged by size:
6720 683 56
BtsI GCAGTG_nn'
Cuts at: 3050 3594 4975 5769 5789 3050
Size: 544 1381 794 20 4720
Fragments arranged by size:
4720 1381 794 544 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7459
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4255 4290 529
Size: 35 3691 35 3698
Fragments arranged by size:
3698 3691 35 35
DraI TTT'AAA
Cuts at: 4763 4763
Size: 7459
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6466 809
Size: 507 5150 1802
Fragments arranged by size:
5150 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2997 3336 4005
Size: 54 1425 50 275 18 1167 339 669
Cuts at: 4005 4906 5795 7424 8
Size: 901 889 1629 43
Fragments arranged by size:
1629 1425 1167 901 889 669 339 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4906 1537
Size: 275 3094 4090
Fragments arranged by size:
4090 3094 275
EarI CTCTTCn'nnn_
Cuts at: 1353 5389 1353
Size: 4036 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2600 6046 6420 6566 387
Size: 1064 138 1011 3446 374 146 1280
Fragments arranged by size:
3446 1280 1064 1011 374 146 138
EcoICRI GAG'CTC
Cuts at: 2569 3902 2569
Size: 1333 6126
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2569 2982 3714 5522 6119 6906 7174
Size: 393 937 413 732 1808 597 787 268
Cuts at: 7174 1239
Size: 1524
Fragments arranged by size:
1808 1524 937 787 732 597 413 393
268
EcoO109I rG'GnC_Cy
Cuts at: 2914 3519 4318 2914
Size: 605 799 6055
Fragments arranged by size:
6055 799 605
EcoRI G'AATT_C
Cuts at: 3268 4571 4768 5146 3268
Size: 1303 197 378 5581
Fragments arranged by size:
5581 1303 378 197
FspI TGC'GCA
Cuts at: 5965 5965
Size: 7459
HaeII r_GCGC'y
Cuts at: 1678 3070 6606 1678
Size: 1392 3536 2531
Fragments arranged by size:
3536 2531 1392
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2476 2508 2879 2911 6100
Size: 32 135 32 904 32 371 32 3189
Cuts at: 6100 6132 6174 6206 1373
Size: 32 42 32 2626
Fragments arranged by size:
3189 2626 904 371 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4803 5049 669
Size: 988 281 2865 246 3079
Fragments arranged by size:
3079 2865 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7459
HpaI GTT'AAC
Cuts at: 5049 5049
Size: 7459
KasI G'GCGC_C
Cuts at: 3066 3066
Size: 7459
KpnI G_GTAC'C
Cuts at: 3693 4784 3693
Size: 1091 6368
MfeI C'AATT_G
Cuts at: 5058 5058
Size: 7459
MluI A'CGCG_T
Cuts at: 7335 7335
Size: 7459
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3297 3877 3937 4679 6573 6757 1305
Size: 1992 580 60 742 1894 184 2007
Fragments arranged by size:
2007 1992 1894 742 580 184 60
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2999 3338 10
Size: 54 1425 343 1167 339 4131
Fragments arranged by size:
4131 1425 1167 343 339 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2744 3611 5417 5776 5935 518
Size: 648 1578 867 1806 359 159 2042
Fragments arranged by size:
2042 1806 1578 867 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2058 2302 2406 2617 2714 3025 3774 4875
Size: 452 244 104 211 97 311 749 1101
Cuts at: 4875 5552 6700 6945 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 1101 749 677 452 311 245
244 211 104 97
NarI GG'CG_CC
Cuts at: 3067 3067
Size: 7459
NcoI C'CATG_G
Cuts at: 513 1065 2004 2898 3062 513
Size: 552 939 894 164 4910
Fragments arranged by size:
4910 939 894 552 164
NdeI CA'TA_TG
Cuts at: 387 4425 387
Size: 4038 3421
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7459
NotI GC'GGCC_GC
Cuts at: 4906 4906
Size: 7459
NspI r_CATG'y
Cuts at: 1132 1229 2295 4817 6362 1132
Size: 97 1066 2522 1545 2229
Fragments arranged by size:
2522 2229 1545 1066 97
PasI CC'CwG_GG
Cuts at: 1348 4492 1348
Size: 3144 4315
PciI A'CATG_T
Cuts at: 6358 6358
Size: 7459
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7459
PfoI T'CCnGG_A
Cuts at: 2487 2487
Size: 7459
PmeI GTTT'AAAC
Cuts at: 4763 4763
Size: 7459
PmlI CAC'GTG
Cuts at: 2872 2872
Size: 7459
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5528 5560 7041 7073 5528
Size: 32 1481 32 5914
Fragments arranged by size:
5914 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7459
PsiI TTA'TAA
Cuts at: 5029 5029
Size: 7459
PspOMI G'GGCC_C
Cuts at: 3519 3519
Size: 7459
PstI C_TGCA'G
Cuts at: 830 2913 3045 4811 830
Size: 2083 132 1766 3478
Fragments arranged by size:
3478 2083 1766 132
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7459
PvuII CAG'CTG
Cuts at: 2058 2302 2617 3025 3774 2058
Size: 244 315 408 749 5743
Fragments arranged by size:
5743 749 408 315 244
SacI G_AGCT'C
Cuts at: 2571 3904 2571
Size: 1333 6126
SalI G'TCGA_C
Cuts at: 1936 4801 1936
Size: 2865 4594
SbfI CC_TGCA'GG
Cuts at: 4811 4811
Size: 7459
ScaI AGT'ACT
Cuts at: 2112 2759 5707 7419 2112
Size: 647 2948 1712 2152
Fragments arranged by size:
2948 2152 1712 647
SfcI C'TryA_G
Cuts at: 826 1045 2812 2909 3041 4807 5942 6623 6814
Size: 219 1767 97 132 1766 1135 681 191
Cuts at: 6814 826
Size: 1471
Fragments arranged by size:
1767 1766 1471 1135 681 219 191 132
97
SfoI GGC'GCC
Cuts at: 3068 3068
Size: 7459
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7459
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7459
SmaI CCC'GGG
Cuts at: 1872 2035 4786 1872
Size: 163 2751 4545
Fragments arranged by size:
4545 2751 163
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2651 2723 3154 5563 6464 6726
Size: 197 926 708 72 431 2409 901 262
Cuts at: 6726 820
Size: 1553
Fragments arranged by size:
2409 1553 926 901 708 431 262 197
72
SnaBI TAC'GTA
Cuts at: 493 5250 493
Size: 4757 2702
SpeI A'CTAG_T
Cuts at: 152 3496 152
Size: 3344 4115
SphI G_CATG'C
Cuts at: 1132 4817 1132
Size: 3685 3774
SrfI GCCC'GGGC
Cuts at: 2035 2035
Size: 7459
SspI AAT'ATT
Cuts at: 5 52 2596 4507 5383 5
Size: 47 2544 1911 876 2081
Fragments arranged by size:
2544 2081 1911 876 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7459
StyI C'CwwG_G
Cuts at: 513 1065 2004 2898 3062 3655 4898 513
Size: 552 939 894 164 593 1243 3074
Fragments arranged by size:
3074 1243 939 894 593 552 164
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2768 2935 3843 5486 5503 5656 5841
Size: 1255 1188 167 908 1643 17 153 185
Cuts at: 5841 6286 325
Size: 445 1498
Fragments arranged by size:
1643 1498 1255 1188 908 445 185 167
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2110 2757 2988
Size: 274 80 33 51 1443 133 647 231
Cuts at: 2988 3387 3778 5705 7417 96
Size: 399 391 1927 1712 138
Fragments arranged by size:
1927 1712 1443 647 399 391 274 231
138 133 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2278 2310 2861 2893 3002 3034 7419 7451 2278
Size: 32 551 32 109 32 4385 32 2286
Fragments arranged by size:
4385 2286 551 109 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7459
XbaI T'CTAG_A
Cuts at: 4795 4795
Size: 7459
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2306 2621 2306
Size: 315 7144
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7459
XmaI C'CCGG_G
Cuts at: 1870 2033 4784 1870
Size: 163 2751 4545
Fragments arranged by size:
4545 2751 163
XmnI GAAnn'nnTTC
Cuts at: 5588 5588
Size: 7459
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5267 276
Size: 53 83 186 936 3733 2468
Fragments arranged by size:
3733 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV
FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI
PpuMI PspXI PsrI RsrII SacII SanDI SapI SexAI
SfiI SwaI