Restriction map for
pFN21AE2675
(Circular) (Six-base) MAPSORT of: pFN21AE2675.seq Check: 656 from: 1 to: 5677
pFN21AE2675 5677 bp
With 182 enzymes: SgfI *
February 2, 2017 15:09 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3487 278
Size: 53 83 186 936 1951 2468
Fragments arranged by size:
2468 1951 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3020 1937
Size: 1083 4594
Acc65I G'GTAC_C
Cuts at: 2998 2998
Size: 5677
AclI AA'CG_TT
Cuts at: 1563 3804 4177 1563
Size: 2241 373 3063
Fragments arranged by size:
3063 2241 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2130 2937 3740 5124 5392 1632
Size: 498 807 803 1384 268 1917
Fragments arranged by size:
1917 1384 807 803 498 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5480
AflIII A'CryG_T
Cuts at: 1203 4576 5553 1203
Size: 3373 977 1327
Fragments arranged by size:
3373 1327 977
AgeI A'CCGG_T
Cuts at: 4508 4508
Size: 5677
AhdI GACnn_n'nnGTC
Cuts at: 4406 4406
Size: 5677
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5677
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5677
AlwNI CAG_nnn'CTG
Cuts at: 3420 4992 3420
Size: 1572 4105
ApaLI G'TGCA_C
Cuts at: 3734 4890 3734
Size: 1156 4521
ApoI r'AATT_y
Cuts at: 1493 2596 2647 2665 2986 3212 3364 5473 1493
Size: 1103 51 18 321 226 152 2109 1697
Fragments arranged by size:
2109 1697 1103 321 226 152 51 18
AseI AT'TA_AT
Cuts at: 160 1032 4231 160
Size: 872 3199 1606
Fragments arranged by size:
3199 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5677
AvaI C'yCGr_G
Cuts at: 1870 1943 3002 1870
Size: 73 1059 4545
Fragments arranged by size:
4545 1059 73
BamHI G'GATC_C
Cuts at: 3007 3374 3007
Size: 367 5310
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2011 2998 4453 618
Size: 325 217 627 224 987 1455 1842
Fragments arranged by size:
1842 1455 987 627 325 224 217
BanII G_rGCy'C
Cuts at: 1391 2052 2996 1391
Size: 661 944 4072
Fragments arranged by size:
4072 944 661
BbeI G_GCGC'C
Cuts at: 2015 2015
Size: 5677
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4706
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2103 2137 3868 3902 1903
Size: 34 166 34 1731 34 3678
Fragments arranged by size:
3678 1731 166 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3569 4779 3569
Size: 1210 4467
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5677
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4288 136
Size: 107 122 71 631 605 337 2279 1525
Fragments arranged by size:
2279 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 2632 2906 5672 2632
Size: 274 2766 2637
Fragments arranged by size:
2766 2637 274
BlpI GC'TnA_GC
Cuts at: 3094 3094
Size: 5677
Bme1580I G_kGCm'C
Cuts at: 1790 3738 4894 1790
Size: 1948 1156 2573
Fragments arranged by size:
2573 1948 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5677
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2054 3408 4366
Size: 416 53 83 399 24 637 1354 958
Cuts at: 4366 442
Size: 1753
Fragments arranged by size:
1753 1354 958 637 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5677
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2476 4337 1239
Size: 1237 1861 2579
Fragments arranged by size:
2579 1861 1237
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3802 4667 4965 3802
Size: 865 298 4514
Fragments arranged by size:
4514 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4340 882
Size: 617 2841 2219
Fragments arranged by size:
2841 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3468 493
Size: 711 2264 2702
Fragments arranged by size:
2702 2264 711
BsaBI GATnn'nnATC
Cuts at: 5671 5671
Size: 5677
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2012 2141 3484 3866
Size: 53 83 186 936 479 129 1343 382
Cuts at: 3866 4501 275
Size: 635 1451
Fragments arranged by size:
1451 1343 936 635 479 382 186 129
83 53
BsaWI w'CCGG_w
Cuts at: 4110 4508 4782 4929 5340 4110
Size: 398 274 147 411 4447
Fragments arranged by size:
4447 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2034 2064 1178
Size: 30 165 30 631 30 4791
Fragments arranged by size:
4791 631 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2213 1186
Size: 1027 4650
BseYI C'CCAG_C
Cuts at: 4880 5376 4880
Size: 496 5181
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5104
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3127 3888 4916 1540
Size: 275 186 1126 761 1028 2301
Fragments arranged by size:
2301 1126 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3738 3823 4894 3738
Size: 85 1071 4521
Fragments arranged by size:
4521 1071 85
BsmI GAATG_Cn'
Cuts at: 3188 3281 3188
Size: 93 5584
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2052 2996 3738 3823 4894 1391
Size: 399 262 944 742 85 1071 2174
Fragments arranged by size:
2174 1071 944 742 399 262 85
BspEI T'CCGG_A
Cuts at: 5340 5340
Size: 5677
BspHI T'CATG_A
Cuts at: 2923 3566 5296 2923
Size: 643 1730 3304
Fragments arranged by size:
3304 1730 643
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3032 844
Size: 2188 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3564 1394
Size: 429 1741 3507
Fragments arranged by size:
3507 1741 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4172 4346 65
Size: 1183 2924 174 1396
Fragments arranged by size:
2924 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4321 4508 1700
Size: 110 2511 187 2869
Fragments arranged by size:
2869 2511 187 110
BsrGI T'GTAC_A
Cuts at: 96 2303 96
Size: 2207 3470
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5677
BssSI C'ACGA_G
Cuts at: 3737 4749 3737
Size: 1012 4665
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2789 794
Size: 1995 3682
BstBI TT'CG_AA
Cuts at: 4481 4481
Size: 5677
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5677
BstXI CCAn_nnnn'nTGG
Cuts at: 2080 2080
Size: 5677
BstYI r'GATC_y
Cuts at: 1972 2632 2906 3007 3374 3759 3776 5228 5314
Size: 660 274 101 367 385 17 1452 86
Cuts at: 5314 5326 5672 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 660 385 367 346 274 101
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2190 2190
Size: 5677
BtgI C'CryG_G
Cuts at: 513 1065 2004 2073 513
Size: 552 939 69 4117
Fragments arranged by size:
4117 939 552 69
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4938
Fragments arranged by size:
4938 683 56
BtsI GCAGTG_nn'
Cuts at: 3193 3987 4007 3193
Size: 794 20 4863
Fragments arranged by size:
4863 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5677
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5642
DraI TTT'AAA
Cuts at: 2981 2981
Size: 5677
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4684 809
Size: 507 3368 1802
Fragments arranged by size:
3368 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2082 3124 4013
Size: 54 1425 50 275 18 252 1042 889
Cuts at: 4013 5642 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1042 889 275 252 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3124 1537
Size: 275 1312 4090
Fragments arranged by size:
4090 1312 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2190 2500 3607 1353
Size: 837 310 1107 3423
Fragments arranged by size:
3423 1107 837 310
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4264 4638 4784 387
Size: 1064 138 2675 374 146 1280
Fragments arranged by size:
2675 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2130 2476 2937 3740 4337 5124 5392
Size: 393 498 346 461 803 597 787 268
Cuts at: 5392 1239
Size: 1524
Fragments arranged by size:
1524 803 787 597 498 461 393 346
268
EcoO109I rG'GnC_Cy
Cuts at: 2037 2936 2037
Size: 899 4778
EcoRI G'AATT_C
Cuts at: 2986 3364 2986
Size: 378 5299
EcoRV GAT'ATC
Cuts at: 2879 2879
Size: 5677
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2411 2975 2411
Size: 564 5113
FspI TGC'GCA
Cuts at: 4183 4183
Size: 5677
HaeII r_GCGC'y
Cuts at: 1678 2015 4824 1678
Size: 337 2809 2531
Fragments arranged by size:
2809 2531 337
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2126 2158 2494 2526 4318
Size: 32 135 32 554 32 336 32 1792
Cuts at: 4318 4350 4392 4424 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1792 554 336 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2166 3021 3267 669
Size: 988 281 228 855 246 3079
Fragments arranged by size:
3079 988 855 281 246 228
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5677
HpaI GTT'AAC
Cuts at: 3267 3267
Size: 5677
KasI G'GCGC_C
Cuts at: 2011 2011
Size: 5677
KpnI G_GTAC'C
Cuts at: 3002 3002
Size: 5677
MfeI C'AATT_G
Cuts at: 3276 3276
Size: 5677
MluI A'CGCG_T
Cuts at: 5553 5553
Size: 5677
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4791 4975 1305
Size: 3486 184 2007
Fragments arranged by size:
3486 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3855
Fragments arranged by size:
3855 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3635 3994 4153 518
Size: 648 2469 359 159 2042
Fragments arranged by size:
2469 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2059 2365 2798 3093 3770 4918 5163 1606
Size: 453 306 433 295 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 453 433 306 295 245
NarI GG'CG_CC
Cuts at: 2012 2012
Size: 5677
NcoI C'CATG_G
Cuts at: 513 1065 2004 2073 513
Size: 552 939 69 4117
Fragments arranged by size:
4117 939 552 69
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5677
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5677
NotI GC'GGCC_GC
Cuts at: 3124 3124
Size: 5677
NspI r_CATG'y
Cuts at: 1132 1229 2214 2304 3035 4580 1132
Size: 97 985 90 731 1545 2229
Fragments arranged by size:
2229 1545 985 731 97 90
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5677
PciI A'CATG_T
Cuts at: 4576 4576
Size: 5677
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5677
PmeI GTTT'AAAC
Cuts at: 2981 2981
Size: 5677
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3746 3778 5259 5291 3746
Size: 32 1481 32 4132
Fragments arranged by size:
4132 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2037 2037
Size: 5677
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5677
PsiI TTA'TAA
Cuts at: 3247 3247
Size: 5677
PstI C_TGCA'G
Cuts at: 830 3029 830
Size: 2199 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5677
PvuII CAG'CTG
Cuts at: 2365 2798 2365
Size: 433 5244
SalI G'TCGA_C
Cuts at: 1936 3019 1936
Size: 1083 4594
SbfI CC_TGCA'GG
Cuts at: 3029 3029
Size: 5677
ScaI AGT'ACT
Cuts at: 3925 5637 3925
Size: 1712 3965
SfcI C'TryA_G
Cuts at: 826 1045 2793 3025 4160 4841 5032 826
Size: 219 1748 232 1135 681 191 1471
Fragments arranged by size:
1748 1471 1135 681 232 219 191
SfoI GGC'GCC
Cuts at: 2013 2013
Size: 5677
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5677
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5677
SmaI CCC'GGG
Cuts at: 1872 3004 1872
Size: 1132 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3781 4682 4944 820
Size: 197 926 1838 901 262 1553
Fragments arranged by size:
1838 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3468 493
Size: 2975 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5677
SphI G_CATG'C
Cuts at: 1132 3035 1132
Size: 1903 3774
SspI AAT'ATT
Cuts at: 5 52 3601 5
Size: 47 3549 2081
Fragments arranged by size:
3549 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5677
StyI C'CwwG_G
Cuts at: 513 1065 2004 2073 2079 3116 513
Size: 552 939 69 6 1037 3074
Fragments arranged by size:
3074 1037 939 552 69 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2020 2113 2120 2712 3704 3721 3874
Size: 1255 440 93 7 592 992 17 153
Cuts at: 3874 4059 4504 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 992 592 445 440 185 153
93 17 7
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2303 3923 5635
Size: 274 80 33 51 1443 326 1620 1712
Cuts at: 5635 96
Size: 138
Fragments arranged by size:
1712 1620 1443 326 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2764 2796 5637 5669 2764
Size: 32 2841 32 2772
Fragments arranged by size:
2841 2772 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2123 1316
Size: 807 4870
XbaI T'CTAG_A
Cuts at: 3013 3013
Size: 5677
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5677
XmaI C'CCGG_G
Cuts at: 1870 3002 1870
Size: 1132 4545
XmnI GAAnn'nnTTC
Cuts at: 3806 3806
Size: 5677
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3485 276
Size: 53 83 186 936 1951 2468
Fragments arranged by size:
2468 1951 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI
EcoNI FseI FspAI NaeI NgoMIV NruI NsiI PacI
PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SapI SexAI SfiI SrfI SwaI XcmI