Restriction map for
pFN21AE2669
(Circular) (Six-base) MAPSORT of: pFN21AE2669.seq Check: 5338 from: 1 to: 5767
pFN21AE2669 5767 bp
With 182 enzymes: SgfI *
January 26, 2017 18:09 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3577 278
Size: 53 83 186 936 2041 2468
Fragments arranged by size:
2468 2041 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2872 3110 1937
Size: 935 238 4594
Fragments arranged by size:
4594 935 238
Acc65I G'GTAC_C
Cuts at: 3088 3088
Size: 5767
AclI AA'CG_TT
Cuts at: 1563 3894 4267 1563
Size: 2331 373 3063
Fragments arranged by size:
3063 2331 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3830 5214 5482 1632
Size: 2198 1384 268 1917
Fragments arranged by size:
2198 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5570
AflIII A'CryG_T
Cuts at: 1203 4666 5643 1203
Size: 3463 977 1327
Fragments arranged by size:
3463 1327 977
AgeI A'CCGG_T
Cuts at: 4598 4598
Size: 5767
AhdI GACnn_n'nnGTC
Cuts at: 4496 4496
Size: 5767
AleI CACnn'nnGTG
Cuts at: 1166 2078 2261 1166
Size: 912 183 4672
Fragments arranged by size:
4672 912 183
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5767
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2025 2057 2025
Size: 32 5735
AlwNI CAG_nnn'CTG
Cuts at: 3510 5082 3510
Size: 1572 4195
ApaLI G'TGCA_C
Cuts at: 3824 4980 3824
Size: 1156 4611
ApoI r'AATT_y
Cuts at: 1493 2972 3076 3302 3454 5563 1493
Size: 1479 104 226 152 2109 1697
Fragments arranged by size:
2109 1697 1479 226 152 104
AseI AT'TA_AT
Cuts at: 160 1032 4321 160
Size: 872 3289 1606
Fragments arranged by size:
3289 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5767
AvaI C'yCGr_G
Cuts at: 1870 1943 3092 1870
Size: 73 1149 4545
Fragments arranged by size:
4545 1149 73
BamHI G'GATC_C
Cuts at: 2941 3097 3464 2941
Size: 156 367 5244
Fragments arranged by size:
5244 367 156
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2109 2323 3088 4543 618
Size: 325 217 627 322 214 765 1455 1842
Fragments arranged by size:
1842 1455 765 627 325 322 217 214
BanII G_rGCy'C
Cuts at: 1391 2412 3086 1391
Size: 1021 674 4072
Fragments arranged by size:
4072 1021 674
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2029 2785 928
Size: 971 130 756 3910
Fragments arranged by size:
3910 971 756 130
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2550 2584 3958 3992 1903
Size: 34 613 34 1374 34 3678
Fragments arranged by size:
3678 1374 613 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2051 3659 4869 2051
Size: 1608 1210 2949
Fragments arranged by size:
2949 1608 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5767
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2608 4378 136
Size: 107 122 71 631 605 936 1770 1525
Fragments arranged by size:
1770 1525 936 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2099 5762 2099
Size: 3663 2104
BlpI GC'TnA_GC
Cuts at: 3184 3184
Size: 5767
Bme1580I G_kGCm'C
Cuts at: 1790 2114 3828 4984 1790
Size: 324 1714 1156 2573
Fragments arranged by size:
2573 1714 1156 324
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5767
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3498 4456 442
Size: 416 53 83 399 24 2081 958 1753
Fragments arranged by size:
2081 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5767
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4427 1239
Size: 3188 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2417 2566 3892 4757 5055 2417
Size: 149 1326 865 298 3129
Fragments arranged by size:
3129 1326 865 298 149
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2372 2497 4430 882
Size: 617 873 125 1933 2219
Fragments arranged by size:
2219 1933 873 617 125
BsaAI yAC'GTr
Cuts at: 493 1204 3558 493
Size: 711 2354 2702
Fragments arranged by size:
2702 2354 711
BsaBI GATnn'nnATC
Cuts at: 5761 5761
Size: 5767
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3574 3956 4591 275
Size: 53 83 186 936 2041 382 635 1451
Fragments arranged by size:
2041 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4200 4598 4872 5019 5430 4200
Size: 398 274 147 411 4537
Fragments arranged by size:
4537 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5542
Fragments arranged by size:
5542 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2218 1186
Size: 1032 4735
BseYI C'CCAG_C
Cuts at: 2146 2222 4970 5466 2146
Size: 76 2748 496 2447
Fragments arranged by size:
2748 2447 496 76
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5194
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3217 3978 5006 1540
Size: 275 186 1216 761 1028 2301
Fragments arranged by size:
2301 1216 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3828 3913 4984 3828
Size: 85 1071 4611
Fragments arranged by size:
4611 1071 85
BsmI GAATG_Cn'
Cuts at: 3278 3371 3278
Size: 93 5674
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2114 2412 3086 3828 3913 4984 1391
Size: 399 324 298 674 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 674 399 324 298 85
BspEI T'CCGG_A
Cuts at: 5430 5430
Size: 5767
BspHI T'CATG_A
Cuts at: 3656 5386 3656
Size: 1730 4037
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3122 844
Size: 2278 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2107 3654 1394
Size: 429 284 1547 3507
Fragments arranged by size:
3507 1547 429 284
BsrDI GCAATG_nn'
Cuts at: 65 1248 2073 4262 4436 65
Size: 1183 825 2189 174 1396
Fragments arranged by size:
2189 1396 1183 825 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2174 4411 4598 1700
Size: 110 364 2237 187 2869
Fragments arranged by size:
2869 2237 364 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5767
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5767
BssSI C'ACGA_G
Cuts at: 3827 4839 3827
Size: 1012 4755
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5767
BstBI TT'CG_AA
Cuts at: 4571 4571
Size: 5767
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5767
BstXI CCAn_nnnn'nTGG
Cuts at: 2389 2389
Size: 5767
BstYI r'GATC_y
Cuts at: 1972 2099 2941 3097 3464 3849 3866 5318 5404
Size: 127 842 156 367 385 17 1452 86
Cuts at: 5404 5416 5762 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 842 385 367 346 156 127
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3061 3061
Size: 5767
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5215
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5028
Fragments arranged by size:
5028 683 56
BtsI GCAGTG_nn'
Cuts at: 3283 4077 4097 3283
Size: 794 20 4953
Fragments arranged by size:
4953 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5767
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5732
DraI TTT'AAA
Cuts at: 3071 3071
Size: 5767
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4774 809
Size: 507 3458 1802
Fragments arranged by size:
3458 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2265 3214 4103
Size: 54 1425 50 275 18 435 949 889
Cuts at: 4103 5732 8
Size: 1629 43
Fragments arranged by size:
1629 1425 949 889 435 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3214 1537
Size: 275 1402 4090
Fragments arranged by size:
4090 1402 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3697 1353
Size: 2344 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4354 4728 4874 387
Size: 1064 138 2765 374 146 1280
Fragments arranged by size:
2765 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3830 4427 5214 5482 1239
Size: 393 2198 597 787 268 1524
Fragments arranged by size:
2198 1524 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2048 2048
Size: 5767
EcoO109I rG'GnC_Cy
Cuts at: 2600 2600
Size: 5767
EcoRI G'AATT_C
Cuts at: 3076 3454 3076
Size: 378 5389
FspI TGC'GCA
Cuts at: 4273 4273
Size: 5767
HaeII r_GCGC'y
Cuts at: 1678 4914 1678
Size: 3236 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4408 4440 4482 4514 1373
Size: 32 135 32 2836 32 42 32 2626
Fragments arranged by size:
2836 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3111 3357 669
Size: 988 281 1173 246 3079
Fragments arranged by size:
3079 1173 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5767
HpaI GTT'AAC
Cuts at: 3357 3357
Size: 5767
KpnI G_GTAC'C
Cuts at: 3092 3092
Size: 5767
MfeI C'AATT_G
Cuts at: 3366 3366
Size: 5767
MluI A'CGCG_T
Cuts at: 5643 5643
Size: 5767
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4881 5065 1305
Size: 3576 184 2007
Fragments arranged by size:
3576 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3945
Fragments arranged by size:
3945 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2078 2081 2261 2387 2936 3725 4084
Size: 648 912 3 180 126 549 789 359
Cuts at: 4084 4243 518
Size: 159 2042
Fragments arranged by size:
2042 912 789 648 549 359 180 159
126 3
MspA1I CmG'CkG
Cuts at: 1606 2137 3183 3860 5008 5253 1606
Size: 531 1046 677 1148 245 2120
Fragments arranged by size:
2120 1148 1046 677 531 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5215
NdeI CA'TA_TG
Cuts at: 387 2639 387
Size: 2252 3515
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5767
NotI GC'GGCC_GC
Cuts at: 3214 3214
Size: 5767
NspI r_CATG'y
Cuts at: 1132 1229 3125 4670 1132
Size: 97 1896 1545 2229
Fragments arranged by size:
2229 1896 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5767
PciI A'CATG_T
Cuts at: 4666 4666
Size: 5767
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5767
PmeI GTTT'AAAC
Cuts at: 3071 3071
Size: 5767
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3836 3868 5349 5381 3836
Size: 32 1481 32 4222
Fragments arranged by size:
4222 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5767
PsiI TTA'TAA
Cuts at: 3337 3337
Size: 5767
PstI C_TGCA'G
Cuts at: 830 3119 830
Size: 2289 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5767
PvuII CAG'CTG
Cuts at: 2137 2137
Size: 5767
SalI G'TCGA_C
Cuts at: 1936 3109 1936
Size: 1173 4594
SbfI CC_TGCA'GG
Cuts at: 3119 3119
Size: 5767
ScaI AGT'ACT
Cuts at: 4015 5727 4015
Size: 1712 4055
SfcI C'TryA_G
Cuts at: 826 1045 2869 3115 4250 4931 5122 826
Size: 219 1824 246 1135 681 191 1471
Fragments arranged by size:
1824 1471 1135 681 246 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5767
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5767
SmaI CCC'GGG
Cuts at: 1872 3094 1872
Size: 1222 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2432 2545 3871 4772 5034 820
Size: 197 926 489 113 1326 901 262 1553
Fragments arranged by size:
1553 1326 926 901 489 262 197 113
SnaBI TAC'GTA
Cuts at: 493 3558 493
Size: 3065 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5767
SphI G_CATG'C
Cuts at: 1132 3125 1132
Size: 1993 3774
SspI AAT'ATT
Cuts at: 5 52 2458 3691 5
Size: 47 2406 1233 2081
Fragments arranged by size:
2406 2081 1233 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5767
StyI C'CwwG_G
Cuts at: 513 1065 3206 513
Size: 552 2141 3074
Fragments arranged by size:
3074 2141 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2194 3794 3811 3964 4149 4594 325
Size: 1255 614 1600 17 153 185 445 1498
Fragments arranged by size:
1600 1498 1255 614 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2131 4013 5725
Size: 274 80 33 51 1443 154 1882 1712
Cuts at: 5725 96
Size: 138
Fragments arranged by size:
1882 1712 1443 274 154 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5727 5759 5727
Size: 32 5735
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5767
XbaI T'CTAG_A
Cuts at: 3103 3103
Size: 5767
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5767
XmaI C'CCGG_G
Cuts at: 1870 3092 1870
Size: 1222 4545
XmnI GAAnn'nnTTC
Cuts at: 3896 3896
Size: 5767
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3575 276
Size: 53 83 186 936 2041 2468
Fragments arranged by size:
2468 2041 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI