Restriction map for
pFN21AE2651
(Circular) (Six-base) MAPSORT of: pFN21AE2651.seq Check: 9795 from: 1 to: 7186
pFN21AE2651 7186 bp
With 182 enzymes: SgfI *
November 16, 2016 15:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3196 3196
Size: 7186
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2412 4996 278
Size: 53 83 186 936 876 2584 2468
Fragments arranged by size:
2584 2468 936 876 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3498 4529 1937
Size: 1561 1031 4594
Fragments arranged by size:
4594 1561 1031
Acc65I G'GTAC_C
Cuts at: 3235 4507 3235
Size: 1272 5914
AclI AA'CG_TT
Cuts at: 1563 5313 5686 1563
Size: 3750 373 3063
Fragments arranged by size:
3750 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2291 2732 2903 4208 5249 6633 6901 1632
Size: 659 441 171 1305 1041 1384 268 1917
Fragments arranged by size:
1917 1384 1305 1041 659 441 268 171
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6989
AflIII A'CryG_T
Cuts at: 1203 2892 4257 6085 7062 1203
Size: 1689 1365 1828 977 1327
Fragments arranged by size:
1828 1689 1365 1327 977
AgeI A'CCGG_T
Cuts at: 6017 6017
Size: 7186
AhdI GACnn_n'nnGTC
Cuts at: 5915 5915
Size: 7186
AleI CACnn'nnGTG
Cuts at: 1166 2735 2990 1166
Size: 1569 255 5362
Fragments arranged by size:
5362 1569 255
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7186
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3935 3967 3935
Size: 32 7154
AlwNI CAG_nnn'CTG
Cuts at: 2419 2839 4929 6501 2419
Size: 420 2090 1572 3104
Fragments arranged by size:
3104 2090 1572 420
ApaI G_GGCC'C
Cuts at: 3543 3543
Size: 7186
ApaLI G'TGCA_C
Cuts at: 5243 6399 5243
Size: 1156 6030
ApoI r'AATT_y
Cuts at: 1493 4495 4721 4873 6982 1493
Size: 3002 226 152 2109 1697
Fragments arranged by size:
3002 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5740 160
Size: 872 4708 1606
Fragments arranged by size:
4708 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7186
AvaI C'yCGr_G
Cuts at: 1870 1943 2053 4346 4511 1870
Size: 73 110 2293 165 4545
Fragments arranged by size:
4545 2293 165 110 73
AvrII C'CTAG_G
Cuts at: 3076 3076
Size: 7186
BamHI G'GATC_C
Cuts at: 4147 4516 4883 4147
Size: 369 367 6450
Fragments arranged by size:
6450 369 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2092 3235 3509 3575 4507
Size: 325 217 627 305 1143 274 66 932
Cuts at: 4507 5962 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1143 932 627 325 305 274
217 66
BanII G_rGCy'C
Cuts at: 1391 3101 3543 4116 4344 4505 1391
Size: 1710 442 573 228 161 4072
Fragments arranged by size:
4072 1710 573 442 228 161
BbeI G_GCGC'C
Cuts at: 3579 3579
Size: 7186
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3970 928
Size: 971 2071 4144
Fragments arranged by size:
4144 2071 971
BbvCI CC'TCA_GC
Cuts at: 4233 4233
Size: 7186
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5377 5411 1903
Size: 34 3440 34 3678
Fragments arranged by size:
3678 3440 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5078 6288 5078
Size: 1210 5976
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7186
BfrBI ATG'CAT
Cuts at: 3145 3145
Size: 7186
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5797 136
Size: 107 122 71 631 605 4125 1525
Fragments arranged by size:
4125 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2886 7181 2886
Size: 4295 2891
BlpI GC'TnA_GC
Cuts at: 2015 4603 2015
Size: 2588 4598
Bme1580I G_kGCm'C
Cuts at: 1790 2727 3543 5247 6403 1790
Size: 937 816 1704 1156 2573
Fragments arranged by size:
2573 1704 1156 937 816
BmgBI CAC'GTC
Cuts at: 1318 4258 1318
Size: 2940 4246
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3649 3897 4917
Size: 416 53 83 399 24 2232 248 1020
Cuts at: 4917 5875 442
Size: 958 1753
Fragments arranged by size:
2232 1753 1020 958 416 399 248 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7186
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2416 2416
Size: 7186
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2475 4237 4331 4404 5846 1239
Size: 1236 1762 94 73 1442 2579
Fragments arranged by size:
2579 1762 1442 1236 94 73
Bpu10I CC'TnA_GC
Cuts at: 4233 4233
Size: 7186
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2735 3632 4034 4448 5311 6176 6474 2735
Size: 897 402 414 863 865 298 3447
Fragments arranged by size:
3447 897 865 863 414 402 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2387 2592 3527 4073 5849 882
Size: 617 888 205 935 546 1776 2219
Fragments arranged by size:
2219 1776 935 888 617 546 205
BsaAI yAC'GTr
Cuts at: 493 1204 2389 2689 2893 4977 493
Size: 711 1185 300 204 2084 2702
Fragments arranged by size:
2702 2084 1185 711 300 204
BsaBI GATnn'nnATC
Cuts at: 7180 7180
Size: 7186
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2409 3576 4993 5375
Size: 53 83 186 936 876 1167 1417 382
Cuts at: 5375 6010 275
Size: 635 1451
Fragments arranged by size:
1451 1417 1167 936 876 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2355 2542 3063 5619 6017 6291 6438 6849 2355
Size: 187 521 2556 398 274 147 411 2692
Fragments arranged by size:
2692 2556 521 411 398 274 187 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3719 3749 1178
Size: 30 165 30 2316 30 4615
Fragments arranged by size:
4615 2316 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4022 4025 4352 1186
Size: 2836 3 327 4020
Fragments arranged by size:
4020 2836 327 3
BseYI C'CCAG_C
Cuts at: 2692 3168 3604 4061 4361 6389 6885 2692
Size: 476 436 457 300 2028 496 2993
Fragments arranged by size:
2993 2028 496 476 457 436 300
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2306 2742 3020 3224 4020 1163
Size: 573 570 436 278 204 796 4329
Fragments arranged by size:
4329 796 573 570 436 278 204
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2721 3024 4636 5397 6425 1540
Size: 275 186 720 303 1612 761 1028 2301
Fragments arranged by size:
2301 1612 1028 761 720 303 275 186
BsiHKAI G_wGCw'C
Cuts at: 4344 5247 5332 6403 4344
Size: 903 85 1071 5127
Fragments arranged by size:
5127 1071 903 85
BsmI GAATG_Cn'
Cuts at: 4697 4790 4697
Size: 93 7093
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2727 3101 3543 4116 4344 4505 5247
Size: 399 937 374 442 573 228 161 742
Cuts at: 5247 5332 6403 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 937 742 573 442 399 374
228 161 85
BspEI T'CCGG_A
Cuts at: 2355 6849 2355
Size: 4494 2692
BspHI T'CATG_A
Cuts at: 5075 6805 5075
Size: 1730 5456
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2847 3196 3294 3336 4541 844
Size: 2003 349 98 42 1205 3489
Fragments arranged by size:
3489 2003 1205 349 98 42
BsrBI CCG'CTC
Cuts at: 1394 1823 2608 3703 5073 1394
Size: 429 785 1095 1370 3507
Fragments arranged by size:
3507 1370 1095 785 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5681 5855 65
Size: 1183 4433 174 1396
Fragments arranged by size:
4433 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2743 5830 6017 1700
Size: 110 933 3087 187 2869
Fragments arranged by size:
3087 2869 933 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7186
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7186
BssSI C'ACGA_G
Cuts at: 2849 4109 5246 6258 2849
Size: 1260 1137 1012 3777
Fragments arranged by size:
3777 1260 1137 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2092 3328 4034 794
Size: 1298 1236 706 3946
Fragments arranged by size:
3946 1298 1236 706
BstBI TT'CG_AA
Cuts at: 5990 5990
Size: 7186
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7186
BstXI CCAn_nnnn'nTGG
Cuts at: 2089 2257 2537 3269 3572 4093 2089
Size: 168 280 732 303 521 5182
Fragments arranged by size:
5182 732 521 303 280 168
BstYI r'GATC_y
Cuts at: 1972 2592 2886 3432 3909 4147 4516 4883 5268
Size: 620 294 546 477 238 369 367 385
Cuts at: 5268 5285 6737 6823 6835 7181 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 620 546 477 385 369 367
346 294 238 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3070 3156 3070
Size: 86 7100
BtgI C'CryG_G
Cuts at: 513 1065 3102 513
Size: 552 2037 4597
Fragments arranged by size:
4597 2037 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6447
Fragments arranged by size:
6447 683 56
BtsI GCAGTG_nn'
Cuts at: 4032 4227 4702 5496 5516 4032
Size: 195 475 794 20 5702
Fragments arranged by size:
5702 794 475 195 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7186
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7151
DraI TTT'AAA
Cuts at: 4490 4490
Size: 7186
DraIII CAC_nnn'GTG
Cuts at: 3094 3094
Size: 7186
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6193 809
Size: 507 4877 1802
Fragments arranged by size:
4877 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2203 2583 2718
Size: 54 1425 50 275 18 373 380 135
Cuts at: 2718 2822 3021 3105 3711 3900 4633 5522 7151
Size: 104 199 84 606 189 733 889 1629
Cuts at: 7151 8
Size: 43
Fragments arranged by size:
1629 1425 889 733 606 380 373 275
199 189 135 104 84 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2718 3021 4633 1537
Size: 275 906 303 1612 4090
Fragments arranged by size:
4090 1612 906 303 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2471 3044 3933 5116 1353
Size: 1118 573 889 1183 3423
Fragments arranged by size:
3423 1183 1118 889 573
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4208 5773 6147 6293 387
Size: 1064 138 2619 1565 374 146 1280
Fragments arranged by size:
2619 1565 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4342 4342
Size: 7186
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2291 2475 2732 2903 4208 4237 4331
Size: 393 659 184 257 171 1305 29 94
Cuts at: 4331 4404 5249 5846 6633 6901 1239
Size: 73 845 597 787 268 1524
Fragments arranged by size:
1524 1305 845 787 659 597 393 268
257 184 171 94 73 29
EcoNI CCTnn'n_nnAGG
Cuts at: 2870 2870
Size: 7186
EcoO109I rG'GnC_Cy
Cuts at: 2836 2878 3439 3540 3601 3808 4183 2836
Size: 42 561 101 61 207 375 5839
Fragments arranged by size:
5839 561 375 207 101 61 42
EcoRI G'AATT_C
Cuts at: 4495 4873 4495
Size: 378 6808
FspI TGC'GCA
Cuts at: 5692 5692
Size: 7186
HaeII r_GCGC'y
Cuts at: 1678 2022 3579 3895 6333 1678
Size: 344 1557 316 2438 2531
Fragments arranged by size:
2531 2438 1557 344 316
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3122 3154 3719 3751 5827
Size: 32 135 32 1550 32 565 32 2076
Cuts at: 5827 5859 5901 5933 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2076 1550 565 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4530 4776 669
Size: 988 281 2592 246 3079
Fragments arranged by size:
3079 2592 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2119 2519 748
Size: 1371 400 5415
Fragments arranged by size:
5415 1371 400
HpaI GTT'AAC
Cuts at: 4776 4776
Size: 7186
KasI G'GCGC_C
Cuts at: 3575 3575
Size: 7186
KpnI G_GTAC'C
Cuts at: 3239 4511 3239
Size: 1272 5914
MfeI C'AATT_G
Cuts at: 4785 4785
Size: 7186
MluI A'CGCG_T
Cuts at: 7062 7062
Size: 7186
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3084 3903 6300 6484 1305
Size: 1779 819 2397 184 2007
Fragments arranged by size:
2397 2007 1779 819 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2205 2585 3107 3713 3902
Size: 54 1425 343 373 380 522 606 189
Cuts at: 3902 10
Size: 3294
Fragments arranged by size:
3294 1425 606 522 380 373 343 189
54
MslI CAynn'nnrTG
Cuts at: 518 1166 2087 2255 2555 2735 2990 3140 3267
Size: 648 921 168 300 180 255 150 127
Cuts at: 3267 4091 4094 5144 5503 5662 518
Size: 824 3 1050 359 159 2042
Fragments arranged by size:
2042 1050 921 824 648 359 300 255
180 168 159 150 127 3
MspA1I CmG'CkG
Cuts at: 1606 2237 4065 4424 4602 5279 6427 6672 1606
Size: 631 1828 359 178 677 1148 245 2120
Fragments arranged by size:
2120 1828 1148 677 631 359 245 178
NaeI GCC'GGC
Cuts at: 2745 2745
Size: 7186
NarI GG'CG_CC
Cuts at: 3576 3576
Size: 7186
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6634
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7186
NgoMIV G'CCGG_C
Cuts at: 2743 2743
Size: 7186
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7186
NotI GC'GGCC_GC
Cuts at: 4633 4633
Size: 7186
NsiI A_TGCA'T
Cuts at: 3147 3147
Size: 7186
NspI r_CATG'y
Cuts at: 1132 1229 2429 3149 4001 4544 6089 1132
Size: 97 1200 720 852 543 1545 2229
Fragments arranged by size:
2229 1545 1200 852 720 543 97
PasI CC'CwG_GG
Cuts at: 1348 3796 1348
Size: 2448 4738
PciI A'CATG_T
Cuts at: 6085 6085
Size: 7186
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2692 3094 3786 1108
Size: 1584 402 692 4508
Fragments arranged by size:
4508 1584 692 402
PfoI T'CCnGG_A
Cuts at: 2471 2505 2865 3399 3737 2471
Size: 34 360 534 338 5920
Fragments arranged by size:
5920 534 360 338 34
PmeI GTTT'AAAC
Cuts at: 4490 4490
Size: 7186
PmlI CAC'GTG
Cuts at: 2389 2689 2389
Size: 300 6886
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5255 5287 6768 6800 5255
Size: 32 1481 32 5641
Fragments arranged by size:
5641 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2836 3439 3601 4183 2836
Size: 603 162 582 5839
Fragments arranged by size:
5839 603 582 162
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7186
PsiI TTA'TAA
Cuts at: 4756 4756
Size: 7186
PspOMI G'GGCC_C
Cuts at: 3539 3539
Size: 7186
PstI C_TGCA'G
Cuts at: 830 2064 4538 830
Size: 1234 2474 3478
Fragments arranged by size:
3478 2474 1234
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7186
PvuII CAG'CTG
Cuts at: 2237 4424 2237
Size: 2187 4999
SacI G_AGCT'C
Cuts at: 4344 4344
Size: 7186
SalI G'TCGA_C
Cuts at: 1936 4528 1936
Size: 2592 4594
SanDI GG'GwC_CC
Cuts at: 3439 3439
Size: 7186
SapI GCTCTTCn'nnn_
Cuts at: 3044 3044
Size: 7186
SbfI CC_TGCA'GG
Cuts at: 4538 4538
Size: 7186
ScaI AGT'ACT
Cuts at: 5434 7146 5434
Size: 1712 5474
SexAI A'CCwGG_T
Cuts at: 3846 3972 3846
Size: 126 7060
SfcI C'TryA_G
Cuts at: 826 1045 2060 3833 4332 4534 5669 6350 6541
Size: 219 1015 1773 499 202 1135 681 191
Cuts at: 6541 826
Size: 1471
Fragments arranged by size:
1773 1471 1135 1015 681 499 219 202
191
SfoI GGC'GCC
Cuts at: 3577 3577
Size: 7186
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7186
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7186
SmaI CCC'GGG
Cuts at: 1872 2055 4513 1872
Size: 183 2458 4545
Fragments arranged by size:
4545 2458 183
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2750 3647 4049 4463 5290 6191
Size: 197 926 807 897 402 414 827 901
Cuts at: 6191 6453 820
Size: 262 1553
Fragments arranged by size:
1553 926 901 897 827 807 414 402
262 197
SnaBI TAC'GTA
Cuts at: 493 4977 493
Size: 4484 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7186
SphI G_CATG'C
Cuts at: 1132 4544 1132
Size: 3412 3774
SspI AAT'ATT
Cuts at: 5 52 5110 5
Size: 47 5058 2081
Fragments arranged by size:
5058 2081 47
StuI AGG'CCT
Cuts at: 1517 2321 2346 3075 3593 4406 1517
Size: 804 25 729 518 813 4297
Fragments arranged by size:
4297 813 804 729 518 25
StyI C'CwwG_G
Cuts at: 513 1065 2512 2586 3076 3588 4625 513
Size: 552 1447 74 490 512 1037 3074
Fragments arranged by size:
3074 1447 1037 552 512 490 74
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3788 5213 5230 5383 5568 6013 325
Size: 1255 2208 1425 17 153 185 445 1498
Fragments arranged by size:
2208 1498 1425 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3411 5432 7144
Size: 274 80 33 51 1443 1434 2021 1712
Cuts at: 7144 96
Size: 138
Fragments arranged by size:
2021 1712 1443 1434 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3236 3268 3477 3509 3540 3572 7146 7178 3236
Size: 32 209 32 31 32 3574 32 3244
Fragments arranged by size:
3574 3244 209 32 32 32 32 31
Tth111I GACn'n_nGTC
Cuts at: 1316 3055 3655 3974 1316
Size: 1739 600 319 4528
Fragments arranged by size:
4528 1739 600 319
XbaI T'CTAG_A
Cuts at: 4522 4522
Size: 7186
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2198 3413 3783 3787 4306 2198
Size: 1215 370 4 519 5078
Fragments arranged by size:
5078 1215 519 370 4
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7186
XmaI C'CCGG_G
Cuts at: 1870 2053 4511 1870
Size: 183 2458 4545
Fragments arranged by size:
4545 2458 183
XmnI GAAnn'nnTTC
Cuts at: 2480 5315 2480
Size: 2835 4351
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2410 4994 276
Size: 53 83 186 936 876 2584 2468
Fragments arranged by size:
2584 2468 936 876 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SalI SanDI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AscI BaeI BsiWI BsmBI BstZ17I EcoRV FalI
FseI FspAI NruI PacI PspXI PsrI RsrII SacII
SfiI SrfI SwaI