Restriction map for
pFN21AE2647
(Circular) (Six-base) MAPSORT of: pFN21AE2647.seq Check: 2550 from: 1 to: 6181
pFN21AE2647 6181 bp
With 182 enzymes: SgfI *
November 11, 2016 17:48 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2557 2557
Size: 6181
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3991 278
Size: 53 83 186 936 2455 2468
Fragments arranged by size:
2468 2455 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3524 1937
Size: 1587 4594
Acc65I G'GTAC_C
Cuts at: 3502 3502
Size: 6181
AclI AA'CG_TT
Cuts at: 1563 4308 4681 1563
Size: 2745 373 3063
Fragments arranged by size:
3063 2745 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2129 4244 5628 5896 1632
Size: 497 2115 1384 268 1917
Fragments arranged by size:
2115 1917 1384 497 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5984
AflIII A'CryG_T
Cuts at: 1203 5080 6057 1203
Size: 3877 977 1327
Fragments arranged by size:
3877 1327 977
AgeI A'CCGG_T
Cuts at: 5012 5012
Size: 6181
AhdI GACnn_n'nnGTC
Cuts at: 4910 4910
Size: 6181
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6181
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2543 2846 1184
Size: 1359 303 4519
Fragments arranged by size:
4519 1359 303
AlwNI CAG_nnn'CTG
Cuts at: 2628 3307 3924 5496 2628
Size: 679 617 1572 3313
Fragments arranged by size:
3313 1572 679 617
ApaI G_GGCC'C
Cuts at: 2768 2768
Size: 6181
ApaLI G'TGCA_C
Cuts at: 2288 2570 4238 5394 2288
Size: 282 1668 1156 3075
Fragments arranged by size:
3075 1668 1156 282
ApoI r'AATT_y
Cuts at: 1493 3490 3716 3868 5977 1493
Size: 1997 226 152 2109 1697
Fragments arranged by size:
2109 1997 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4735 160
Size: 872 3703 1606
Fragments arranged by size:
3703 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6181
AvaI C'yCGr_G
Cuts at: 1870 1943 2012 2054 2561 3101 3506 1870
Size: 73 69 42 507 540 405 4545
Fragments arranged by size:
4545 540 507 405 73 69 42
BamHI G'GATC_C
Cuts at: 3511 3878 3511
Size: 367 5814
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2453 2822 3241 3502 4957
Size: 325 217 627 666 369 419 261 1455
Cuts at: 4957 618
Size: 1842
Fragments arranged by size:
1842 1455 666 627 419 369 325 261
217
BanII G_rGCy'C
Cuts at: 1391 2013 2141 2562 2768 3061 3500 1391
Size: 622 128 421 206 293 439 4072
Fragments arranged by size:
4072 622 439 421 293 206 128
BbeI G_GCGC'C
Cuts at: 2457 2457
Size: 6181
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2616 928
Size: 971 717 4493
Fragments arranged by size:
4493 971 717
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4372 4406 1903
Size: 34 2435 34 3678
Fragments arranged by size:
3678 2435 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4073 5283 4073
Size: 1210 4971
BclI T'GATC_A
Cuts at: 1548 2436 1548
Size: 888 5293
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2399 2654 2763
Size: 107 122 71 631 605 727 255 109
Cuts at: 2763 2777 2939 2948 3026 3173 4792 136
Size: 14 162 9 78 147 1619 1525
Fragments arranged by size:
1619 1525 727 631 605 255 162 147
122 109 107 78 71 14 9
BglII A'GATC_T
Cuts at: 3327 6176 3327
Size: 2849 3332
BlpI GC'TnA_GC
Cuts at: 3598 3598
Size: 6181
Bme1580I G_kGCm'C
Cuts at: 1790 2267 2292 2574 2768 4242 5398 1790
Size: 477 25 282 194 1474 1156 2573
Fragments arranged by size:
2573 1474 1156 477 282 194 25
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6181
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3912 4870 442
Size: 416 53 83 399 24 2495 958 1753
Fragments arranged by size:
2495 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6181
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4841 1239
Size: 3602 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4306 5171 5469 4306
Size: 865 298 5018
Fragments arranged by size:
5018 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2180 4844 882
Size: 617 681 2664 2219
Fragments arranged by size:
2664 2219 681 617
BsaAI yAC'GTr
Cuts at: 493 1204 3972 493
Size: 711 2768 2702
Fragments arranged by size:
2768 2702 711
BsaBI GATnn'nnATC
Cuts at: 6175 6175
Size: 6181
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2454 2925 3988 4370
Size: 53 83 186 936 921 471 1063 382
Cuts at: 4370 5005 275
Size: 635 1451
Fragments arranged by size:
1451 1063 936 921 635 471 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2189 4614 5012 5286 5433 5844 2189
Size: 2425 398 274 147 411 2526
Fragments arranged by size:
2526 2425 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2478 2508 2674 2704 2870
Size: 30 165 30 1075 30 166 30 166
Cuts at: 2870 2900 1178
Size: 30 4459
Fragments arranged by size:
4459 1075 166 166 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6181
BseYI C'CCAG_C
Cuts at: 2123 2761 2956 5384 5880 2123
Size: 638 195 2428 496 2424
Fragments arranged by size:
2428 2424 638 496 195
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2050 2192 2516 2615 1163
Size: 573 314 142 324 99 4729
Fragments arranged by size:
4729 573 324 314 142 99
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2730 2742 2913 3168 3177 3631
Size: 275 186 729 12 171 255 9 454
Cuts at: 3631 4392 5420 1540
Size: 761 1028 2301
Fragments arranged by size:
2301 1028 761 729 454 275 255 186
171 12 9
BsiHKAI G_wGCw'C
Cuts at: 2292 2574 4242 4327 5398 2292
Size: 282 1668 85 1071 3075
Fragments arranged by size:
3075 1668 1071 282 85
BsiWI C'GTAC_G
Cuts at: 2694 2694
Size: 6181
BsmI GAATG_Cn'
Cuts at: 2287 3692 3785 2287
Size: 1405 93 4683
Fragments arranged by size:
4683 1405 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2013 2141 2267 2292 2562 2574 2768
Size: 399 223 128 126 25 270 12 194
Cuts at: 2768 3061 3500 4242 4327 5398 1391
Size: 293 439 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 439 399 293 270 223
194 128 126 85 25 12
BspEI T'CCGG_A
Cuts at: 5844 5844
Size: 6181
BspHI T'CATG_A
Cuts at: 4070 5800 4070
Size: 1730 4451
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2535 2557 2838 3536 844
Size: 1691 22 281 698 3489
Fragments arranged by size:
3489 1691 698 281 22
BsrBI CCG'CTC
Cuts at: 1394 1823 2245 3410 4068 1394
Size: 429 422 1165 658 3507
Fragments arranged by size:
3507 1165 658 429 422
BsrDI GCAATG_nn'
Cuts at: 65 1248 4676 4850 65
Size: 1183 3428 174 1396
Fragments arranged by size:
3428 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2684 3366 4825 5012 1700
Size: 110 874 682 1459 187 2869
Fragments arranged by size:
2869 1459 874 682 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6181
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6181
BssSI C'ACGA_G
Cuts at: 4241 5253 4241
Size: 1012 5169
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2628 794
Size: 1834 4347
BstBI TT'CG_AA
Cuts at: 4985 4985
Size: 6181
BstEII G'GTnAC_C
Cuts at: 1184 3373 3433 1184
Size: 2189 60 3932
Fragments arranged by size:
3932 2189 60
BstXI CCAn_nnnn'nTGG
Cuts at: 2890 2983 3226 2890
Size: 93 243 5845
Fragments arranged by size:
5845 243 93
BstYI r'GATC_y
Cuts at: 1972 3327 3511 3878 4263 4280 5732 5818 5830
Size: 1355 184 367 385 17 1452 86 12
Cuts at: 5830 6176 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1355 385 367 346 184 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2343 2343
Size: 6181
BtgI C'CryG_G
Cuts at: 513 1065 2004 2457 2664 2706 2724 2937 3162
Size: 552 939 453 207 42 18 213 225
Cuts at: 3162 3393 513
Size: 231 3301
Fragments arranged by size:
3301 939 552 453 231 225 213 207
42 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2345 487
Size: 683 56 1119 4323
Fragments arranged by size:
4323 1119 683 56
BtsI GCAGTG_nn'
Cuts at: 3697 4491 4511 3697
Size: 794 20 5367
Fragments arranged by size:
5367 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6181
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6146
DraI TTT'AAA
Cuts at: 3485 3485
Size: 6181
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5188 809
Size: 507 3872 1802
Fragments arranged by size:
3872 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2667 2727 2739
Size: 54 1425 50 275 18 837 60 12
Cuts at: 2739 2835 2910 2931 2940 3078 3150 3165 3174
Size: 96 75 21 9 138 72 15 9
Cuts at: 3174 3396 3628 4517 6146 8
Size: 222 232 889 1629 43
Fragments arranged by size:
1629 1425 889 837 275 232 222 138
96 75 72 60 54 50 43 21
18 15 12 9 9
EagI C'GGCC_G
Cuts at: 1537 1812 2727 2739 2910 3165 3174 3628 1537
Size: 275 915 12 171 255 9 454 4090
Fragments arranged by size:
4090 915 454 275 255 171 12 9
EarI CTCTTCn'nnn_
Cuts at: 1353 4111 1353
Size: 2758 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3144 3209 4768 5142 5288 387
Size: 1064 138 1555 65 1559 374 146 1280
Fragments arranged by size:
1559 1555 1280 1064 374 146 138 65
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2129 4244 4841 5628 5896 1239
Size: 393 497 2115 597 787 268 1524
Fragments arranged by size:
2115 1524 787 597 497 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2258 3272 2258
Size: 1014 5167
EcoO109I rG'GnC_Cy
Cuts at: 2017 2037 2660 2765 2790 3066 3456 2017
Size: 20 623 105 25 276 390 4742
Fragments arranged by size:
4742 623 390 276 105 25 20
EcoRI G'AATT_C
Cuts at: 3490 3868 3490
Size: 378 5803
FspI TGC'GCA
Cuts at: 4687 4687
Size: 6181
HaeII r_GCGC'y
Cuts at: 1678 2457 2589 2852 3318 5328 1678
Size: 779 132 263 466 2010 2531
Fragments arranged by size:
2531 2010 779 466 263 132
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2184 2216 3301 3333 4822
Size: 32 135 32 612 32 1085 32 1489
Cuts at: 4822 4854 4896 4928 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1489 1085 612 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2353 3525 3771 669
Size: 988 281 415 1172 246 3079
Fragments arranged by size:
3079 1172 988 415 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6181
HpaI GTT'AAC
Cuts at: 3771 3771
Size: 6181
KasI G'GCGC_C
Cuts at: 2453 2453
Size: 6181
KpnI G_GTAC'C
Cuts at: 3506 3506
Size: 6181
MfeI C'AATT_G
Cuts at: 3780 3780
Size: 6181
MluI A'CGCG_T
Cuts at: 6057 6057
Size: 6181
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2700 5295 5479 1305
Size: 1395 2595 184 2007
Fragments arranged by size:
2595 2007 1395 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2669 2837 10
Size: 54 1425 343 837 168 3354
Fragments arranged by size:
3354 1425 837 343 168 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4139 4498 4657 518
Size: 648 2973 359 159 2042
Fragments arranged by size:
2973 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2176 2500 2553 2628 2708 2726 3145 3155
Size: 570 324 53 75 80 18 419 10
Cuts at: 3155 3164 3395 3430 3597 4274 5422 5667 1606
Size: 9 231 35 167 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 570 419 324 245 231
167 80 75 53 35 18 10 9
NarI GG'CG_CC
Cuts at: 2454 2454
Size: 6181
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4690
Fragments arranged by size:
4690 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6181
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6181
NotI GC'GGCC_GC
Cuts at: 2727 3628 2727
Size: 901 5280
NspI r_CATG'y
Cuts at: 1132 1229 3539 5084 1132
Size: 97 2310 1545 2229
Fragments arranged by size:
2310 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2890 3061 1348
Size: 1542 171 4468
Fragments arranged by size:
4468 1542 171
PciI A'CATG_T
Cuts at: 5080 5080
Size: 6181
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3475 1108
Size: 2367 3814
PfoI T'CCnGG_A
Cuts at: 3052 3052
Size: 6181
PmeI GTTT'AAAC
Cuts at: 3485 3485
Size: 6181
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4250 4282 5763 5795 4250
Size: 32 1481 32 4636
Fragments arranged by size:
4636 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3066 3066
Size: 6181
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6181
PsiI TTA'TAA
Cuts at: 3751 3751
Size: 6181
PspOMI G'GGCC_C
Cuts at: 2764 2764
Size: 6181
PstI C_TGCA'G
Cuts at: 830 2181 2532 3160 3533 830
Size: 1351 351 628 373 3478
Fragments arranged by size:
3478 1351 628 373 351
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6181
PvuII CAG'CTG
Cuts at: 2176 2500 2628 2176
Size: 324 128 5729
Fragments arranged by size:
5729 324 128
SacII CC_GC'GG
Cuts at: 2709 2727 3165 3396 2709
Size: 18 438 231 5494
Fragments arranged by size:
5494 438 231 18
SalI G'TCGA_C
Cuts at: 1936 3523 1936
Size: 1587 4594
SbfI CC_TGCA'GG
Cuts at: 2532 3533 2532
Size: 1001 5180
ScaI AGT'ACT
Cuts at: 4429 6141 4429
Size: 1712 4469
SexAI A'CCwGG_T
Cuts at: 3252 3252
Size: 6181
SfcI C'TryA_G
Cuts at: 826 1045 2177 2528 3156 3529 4664 5345 5536
Size: 219 1132 351 628 373 1135 681 191
Cuts at: 5536 826
Size: 1471
Fragments arranged by size:
1471 1135 1132 681 628 373 351 219
191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2763 2939 2948 3173 2763
Size: 176 9 225 5771
Fragments arranged by size:
5771 225 176 9
SfoI GGC'GCC
Cuts at: 2455 2455
Size: 6181
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6181
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6181
SmaI CCC'GGG
Cuts at: 1872 3508 1872
Size: 1636 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4285 5186 5448 820
Size: 197 926 2342 901 262 1553
Fragments arranged by size:
2342 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3972 493
Size: 3479 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6181
SphI G_CATG'C
Cuts at: 1132 3539 1132
Size: 2407 3774
SspI AAT'ATT
Cuts at: 5 52 4105 5
Size: 47 4053 2081
Fragments arranged by size:
4053 2081 47
StuI AGG'CCT
Cuts at: 1517 2276 1517
Size: 759 5422
StyI C'CwwG_G
Cuts at: 513 1065 2004 3219 3620 513
Size: 552 939 1215 401 3074
Fragments arranged by size:
3074 1215 939 552 401
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2482 4208 4225 4378 4563 5008 325
Size: 1255 902 1726 17 153 185 445 1498
Fragments arranged by size:
1726 1498 1255 902 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2257 4427 6139
Size: 274 80 33 51 1443 280 2170 1712
Cuts at: 6139 96
Size: 138
Fragments arranged by size:
2170 1712 1443 280 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2478 2510 2674 2706 6141 6173 2478
Size: 32 164 32 3435 32 2486
Fragments arranged by size:
3435 2486 164 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6181
XbaI T'CTAG_A
Cuts at: 3517 3517
Size: 6181
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2344 3226 2344
Size: 882 5299
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6181
XmaI C'CCGG_G
Cuts at: 1870 3506 1870
Size: 1636 4545
XmnI GAAnn'nnTTC
Cuts at: 4310 4310
Size: 6181
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3989 276
Size: 53 83 186 936 2455 2468
Fragments arranged by size:
2468 2455 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacII SalI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsmBI BstZ17I DraIII EcoICRI EcoRV FalI FseI
FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI
PsrI RsrII SacI SanDI SapI SrfI SwaI