Restriction map for
pFN21AE2645
(Circular) (Six-base) MAPSORT of: pFN21AE2645.seq Check: 7072 from: 1 to: 6619
pFN21AE2645 6619 bp
With 182 enzymes: SgfI *
November 16, 2016 15:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4429 278
Size: 53 83 186 936 2893 2468
Fragments arranged by size:
2893 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3962 1937
Size: 2025 4594
Acc65I G'GTAC_C
Cuts at: 3224 3940 3224
Size: 716 5903
AclI AA'CG_TT
Cuts at: 1563 4746 5119 1563
Size: 3183 373 3063
Fragments arranged by size:
3183 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3408 4682 6066 6334 1632
Size: 1776 1274 1384 268 1917
Fragments arranged by size:
1917 1776 1384 1274 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6422
AflIII A'CryG_T
Cuts at: 1203 2777 3754 5518 6495 1203
Size: 1574 977 1764 977 1327
Fragments arranged by size:
1764 1574 1327 977 977
AgeI A'CCGG_T
Cuts at: 5450 5450
Size: 6619
AhdI GACnn_n'nnGTC
Cuts at: 5348 5348
Size: 6619
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6619
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6619
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3741 3773 3741
Size: 32 6587
AlwNI CAG_nnn'CTG
Cuts at: 2023 3767 4362 5934 2023
Size: 1744 595 1572 2708
Fragments arranged by size:
2708 1744 1572 595
ApaLI G'TGCA_C
Cuts at: 4676 5832 4676
Size: 1156 5463
ApoI r'AATT_y
Cuts at: 1493 2140 2159 3008 3035 3928 4154 4306 6415
Size: 647 19 849 27 893 226 152 2109
Cuts at: 6415 1493
Size: 1697
Fragments arranged by size:
2109 1697 893 849 647 226 152 27
19
AseI AT'TA_AT
Cuts at: 160 1032 5173 160
Size: 872 4141 1606
Fragments arranged by size:
4141 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6619
AvaI C'yCGr_G
Cuts at: 1870 1943 3511 3944 1870
Size: 73 1568 433 4545
Fragments arranged by size:
4545 1568 433 73
AvrII C'CTAG_G
Cuts at: 2239 3133 2239
Size: 894 5725
BamHI G'GATC_C
Cuts at: 3949 4316 3949
Size: 367 6252
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3224 3940 5395 618
Size: 325 217 627 1437 716 1455 1842
Fragments arranged by size:
1842 1455 1437 716 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3938 1391
Size: 2547 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5648
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3446 3480 3826 3860 4810 4844 1903
Size: 34 1509 34 346 34 950 34 3678
Fragments arranged by size:
3678 1509 950 346 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2364 4511 5721 2364
Size: 2147 1210 3262
Fragments arranged by size:
3262 2147 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6619
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5230 136
Size: 107 122 71 631 605 3558 1525
Fragments arranged by size:
3558 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2938 3268 6614 2938
Size: 330 3346 2943
Fragments arranged by size:
3346 2943 330
BlpI GC'TnA_GC
Cuts at: 3240 3428 4036 3240
Size: 188 608 5823
Fragments arranged by size:
5823 608 188
Bme1580I G_kGCm'C
Cuts at: 1790 4680 5836 1790
Size: 2890 1156 2573
Fragments arranged by size:
2890 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6619
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2508 2897 3336
Size: 416 53 83 399 24 1091 389 439
Cuts at: 3336 4350 5308 442
Size: 1014 958 1753
Fragments arranged by size:
1753 1091 1014 958 439 416 399 389
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6619
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3244 3886 3244
Size: 642 5977
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2125 2594 3209 5279 1239
Size: 886 469 615 2070 2579
Fragments arranged by size:
2579 2070 886 615 469
Bpu10I CC'TnA_GC
Cuts at: 2090 2181 2090
Size: 91 6528
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2262 2274 2930 4744 5609 5907 2262
Size: 12 656 1814 865 298 2974
Fragments arranged by size:
2974 1814 865 656 298 12
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3571 5282 882
Size: 617 2072 1711 2219
Fragments arranged by size:
2219 2072 1711 617
BsaAI yAC'GTr
Cuts at: 493 1204 4410 493
Size: 711 3206 2702
Fragments arranged by size:
3206 2702 711
BsaBI GATnn'nnATC
Cuts at: 2937 6613 2937
Size: 3676 2943
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4426 4808 5443 275
Size: 53 83 186 936 2893 382 635 1451
Fragments arranged by size:
2893 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5052 5450 5724 5871 6282 5052
Size: 398 274 147 411 5389
Fragments arranged by size:
5389 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3741 3771 3776 3806 1178
Size: 30 165 30 2338 30 5 30 3991
Fragments arranged by size:
3991 2338 165 30 30 30 30 5
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2933 2978 3636 1186
Size: 1747 45 658 4169
Fragments arranged by size:
4169 1747 658 45
BseYI C'CCAG_C
Cuts at: 2324 3731 5822 6318 2324
Size: 1407 2091 496 2625
Fragments arranged by size:
2625 2091 1407 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6046
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4069 4830 5858 1540
Size: 275 186 2068 761 1028 2301
Fragments arranged by size:
2301 2068 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2101 2745 3893 4680 4765 5836 2101
Size: 644 1148 787 85 1071 2884
Fragments arranged by size:
2884 1148 1071 787 644 85
BsmI GAATG_Cn'
Cuts at: 2309 4130 4223 2309
Size: 1821 93 4705
Fragments arranged by size:
4705 1821 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2101 2745 3893 3938 4680 4765 5836
Size: 399 311 644 1148 45 742 85 1071
Cuts at: 5836 1391
Size: 2174
Fragments arranged by size:
2174 1148 1071 742 644 399 311 85
45
BspEI T'CCGG_A
Cuts at: 6282 6282
Size: 6619
BspHI T'CATG_A
Cuts at: 2989 3313 4508 6238 2989
Size: 324 1195 1730 3370
Fragments arranged by size:
3370 1730 1195 324
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3482 3974 844
Size: 2638 492 3489
Fragments arranged by size:
3489 2638 492
BsrBI CCG'CTC
Cuts at: 1394 1823 4506 1394
Size: 429 2683 3507
Fragments arranged by size:
3507 2683 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2404 2460 2548 5114 5288 65
Size: 1183 1156 56 88 2566 174 1396
Fragments arranged by size:
2566 1396 1183 1156 174 88 56
BsrFI r'CCGG_y
Cuts at: 1700 1810 5263 5450 1700
Size: 110 3453 187 2869
Fragments arranged by size:
3453 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6619
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6619
BssSI C'ACGA_G
Cuts at: 4679 5691 4679
Size: 1012 5607
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6619
BstBI TT'CG_AA
Cuts at: 5423 5423
Size: 6619
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6619
BstYI r'GATC_y
Cuts at: 1972 2008 2452 2696 2938 3268 3949 4316 4701
Size: 36 444 244 242 330 681 367 385
Cuts at: 4701 4718 6170 6256 6268 6614 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 681 444 385 367 346 330
244 242 86 36 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2801 513
Size: 552 939 797 4331
Fragments arranged by size:
4331 939 797 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5880
Fragments arranged by size:
5880 683 56
BtsI GCAGTG_nn'
Cuts at: 3112 3723 4135 4929 4949 3112
Size: 611 412 794 20 4782
Fragments arranged by size:
4782 794 611 412 20
ClaI AT'CG_AT
Cuts at: 1554 2931 1554
Size: 1377 5242
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6584
DraI TTT'AAA
Cuts at: 3923 3923
Size: 6619
DraIII CAC_nnn'GTG
Cuts at: 2169 2169
Size: 6619
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5626 809
Size: 507 4310 1802
Fragments arranged by size:
4310 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4066 4955 6584
Size: 54 1425 50 275 18 2236 889 1629
Cuts at: 6584 8
Size: 43
Fragments arranged by size:
2236 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4066 1537
Size: 275 2254 4090
Fragments arranged by size:
4090 2254 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4549 1353
Size: 3196 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5206 5580 5726 387
Size: 1064 138 3617 374 146 1280
Fragments arranged by size:
3617 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2125 2594 3209 3408 4682 5279 6066
Size: 393 493 469 615 199 1274 597 787
Cuts at: 6066 6334 1239
Size: 268 1524
Fragments arranged by size:
1524 1274 787 615 597 493 469 393
268 199
EcoNI CCTnn'n_nnAGG
Cuts at: 3387 3744 3387
Size: 357 6262
EcoO109I rG'GnC_Cy
Cuts at: 2243 3820 2243
Size: 1577 5042
EcoRI G'AATT_C
Cuts at: 3008 3928 4306 3008
Size: 920 378 5321
Fragments arranged by size:
5321 920 378
EcoRV GAT'ATC
Cuts at: 2515 2515
Size: 6619
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2073 2073
Size: 6619
FspI TGC'GCA
Cuts at: 5125 5125
Size: 6619
HaeII r_GCGC'y
Cuts at: 1678 5766 1678
Size: 4088 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3137 3169 3776 3808 5260
Size: 32 135 32 1565 32 607 32 1452
Cuts at: 5260 5292 5334 5366 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1565 1452 607 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2028 3402 3963 4209 669
Size: 988 281 90 1374 561 246 3079
Fragments arranged by size:
3079 1374 988 561 281 246 90
HindIII A'AGCT_T
Cuts at: 748 3679 748
Size: 2931 3688
HpaI GTT'AAC
Cuts at: 4209 4209
Size: 6619
KpnI G_GTAC'C
Cuts at: 3228 3944 3228
Size: 716 5903
MfeI C'AATT_G
Cuts at: 4218 4218
Size: 6619
MluI A'CGCG_T
Cuts at: 6495 6495
Size: 6619
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5733 5917 1305
Size: 4428 184 2007
Fragments arranged by size:
4428 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4797
Fragments arranged by size:
4797 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3018 3638 4577 4936 5095 518
Size: 648 1852 620 939 359 159 2042
Fragments arranged by size:
2042 1852 939 648 620 359 159
MspA1I CmG'CkG
Cuts at: 1606 2020 2254 2337 3168 4035 4712 5860 6105
Size: 414 234 83 831 867 677 1148 245
Cuts at: 6105 1606
Size: 2120
Fragments arranged by size:
2120 1148 867 831 677 414 245 234
83
NcoI C'CATG_G
Cuts at: 513 1065 2004 2801 513
Size: 552 939 797 4331
Fragments arranged by size:
4331 939 797 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6619
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6619
NotI GC'GGCC_GC
Cuts at: 4066 4066
Size: 6619
NspI r_CATG'y
Cuts at: 1132 1229 2781 3553 3758 3977 5522 1132
Size: 97 1552 772 205 219 1545 2229
Fragments arranged by size:
2229 1552 1545 772 219 205 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6619
PciI A'CATG_T
Cuts at: 2777 3754 5518 2777
Size: 977 1764 3878
Fragments arranged by size:
3878 1764 977
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6619
PmeI GTTT'AAAC
Cuts at: 3923 3923
Size: 6619
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3741 3773 4688 4720 6201 6233 3741
Size: 32 915 32 1481 32 4127
Fragments arranged by size:
4127 1481 915 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2243 2243
Size: 6619
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6619
PsiI TTA'TAA
Cuts at: 4189 4189
Size: 6619
PstI C_TGCA'G
Cuts at: 830 3971 830
Size: 3141 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6619
PvuII CAG'CTG
Cuts at: 2020 2254 2337 3168 2020
Size: 234 83 831 5471
Fragments arranged by size:
5471 831 234 83
SalI G'TCGA_C
Cuts at: 1936 3961 1936
Size: 2025 4594
SbfI CC_TGCA'GG
Cuts at: 3971 3971
Size: 6619
ScaI AGT'ACT
Cuts at: 4867 6579 4867
Size: 1712 4907
SfcI C'TryA_G
Cuts at: 826 1045 3967 5102 5783 5974 826
Size: 219 2922 1135 681 191 1471
Fragments arranged by size:
2922 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6619
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6619
SmaI CCC'GGG
Cuts at: 1872 3513 3946 1872
Size: 1641 433 4545
Fragments arranged by size:
4545 1641 433
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2277 2289 2945 4723 5624 5886
Size: 197 926 334 12 656 1778 901 262
Cuts at: 5886 820
Size: 1553
Fragments arranged by size:
1778 1553 926 901 656 334 262 197
12
SnaBI TAC'GTA
Cuts at: 493 4410 493
Size: 3917 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6619
SphI G_CATG'C
Cuts at: 1132 3977 1132
Size: 2845 3774
SspI AAT'ATT
Cuts at: 5 52 2829 4543 5
Size: 47 2777 1714 2081
Fragments arranged by size:
2777 2081 1714 47
StuI AGG'CCT
Cuts at: 1517 2130 3739 1517
Size: 613 1609 4397
Fragments arranged by size:
4397 1609 613
StyI C'CwwG_G
Cuts at: 513 1065 2004 2031 2239 2801 3133 4058 513
Size: 552 939 27 208 562 332 925 3074
Fragments arranged by size:
3074 939 925 562 552 332 208 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2473 4646 4663 4816 5001 5446 325
Size: 1255 893 2173 17 153 185 445 1498
Fragments arranged by size:
2173 1498 1255 893 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2655 3805 4865
Size: 274 80 33 51 1443 678 1150 1060
Cuts at: 4865 6577 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1150 1060 678 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2405 2437 6579 6611 2405
Size: 32 4142 32 2413
Fragments arranged by size:
4142 2413 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6619
XbaI T'CTAG_A
Cuts at: 3955 3955
Size: 6619
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6619
XmaI C'CCGG_G
Cuts at: 1870 3511 3944 1870
Size: 1641 433 4545
Fragments arranged by size:
4545 1641 433
XmnI GAAnn'nnTTC
Cuts at: 3756 4748 3756
Size: 992 5627
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4427 276
Size: 53 83 186 936 2893 2468
Fragments arranged by size:
2893 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI BaeI BbeI BbvCI BfrBI
BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI