Restriction map for
pFN21AE2637
(Circular) (Six-base) MAPSORT of: pFN21AE2637.seq Check: 2285 from: 1 to: 6979
pFN21AE2637 6979 bp
With 182 enzymes: SgfI *
February 20, 2021 22:13 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2676 4789 278
Size: 53 83 186 936 1140 2113 2468
Fragments arranged by size:
2468 2113 1140 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3882 4215 4322 1937
Size: 1945 333 107 4594
Fragments arranged by size:
4594 1945 333 107
Acc65I G'GTAC_C
Cuts at: 4300 4300
Size: 6979
AclI AA'CG_TT
Cuts at: 1563 5106 5479 1563
Size: 3543 373 3063
Fragments arranged by size:
3543 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2334 2633 3734 3773 5042 6426 6694 1632
Size: 702 299 1101 39 1269 1384 268 1917
Fragments arranged by size:
1917 1384 1269 1101 702 299 268 39
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6782
AflIII A'CryG_T
Cuts at: 1203 3194 3850 4161 5878 6855 1203
Size: 1991 656 311 1717 977 1327
Fragments arranged by size:
1991 1717 1327 977 656 311
AgeI A'CCGG_T
Cuts at: 5810 5810
Size: 6979
AhdI GACnn_n'nnGTC
Cuts at: 4249 5708 4249
Size: 1459 5520
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6979
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3099 1184
Size: 1915 5064
AlwNI CAG_nnn'CTG
Cuts at: 2528 4722 6294 2528
Size: 2194 1572 3213
Fragments arranged by size:
3213 2194 1572
ApaI G_GGCC'C
Cuts at: 2492 2865 2995 3023 2492
Size: 373 130 28 6448
Fragments arranged by size:
6448 373 130 28
ApaLI G'TGCA_C
Cuts at: 5036 6192 5036
Size: 1156 5823
ApoI r'AATT_y
Cuts at: 1493 4288 4514 4666 6775 1493
Size: 2795 226 152 2109 1697
Fragments arranged by size:
2795 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3789 5533 160
Size: 872 2757 1744 1606
Fragments arranged by size:
2757 1744 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6979
AvaI C'yCGr_G
Cuts at: 1870 1943 3015 3615 3856 4304 1870
Size: 73 1072 600 241 448 4545
Fragments arranged by size:
4545 1072 600 448 241 73
BamHI G'GATC_C
Cuts at: 4309 4676 4309
Size: 367 6612
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2792 4300 5755 618
Size: 325 217 627 1005 1508 1455 1842
Fragments arranged by size:
1842 1508 1455 1005 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2016 2186 2257 2473 2492 2856 2865 2995
Size: 625 170 71 216 19 364 9 130
Cuts at: 2995 3023 3066 3624 4298 1391
Size: 28 43 558 674 4072
Fragments arranged by size:
4072 674 625 558 364 216 170 130
71 43 28 19 9
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2355 2670 3657 928
Size: 971 456 315 987 4250
Fragments arranged by size:
4250 987 971 456 315
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4235 4269 5170 5204 1903
Size: 34 2298 34 901 34 3678
Fragments arranged by size:
3678 2298 901 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4871 6081 4871
Size: 1210 5769
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6979
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2860 3444 4262
Size: 107 122 71 631 605 1188 584 818
Cuts at: 4262 5590 136
Size: 1328 1525
Fragments arranged by size:
1525 1328 1188 818 631 605 584 122
107 71
BglII A'GATC_T
Cuts at: 6974 6974
Size: 6979
BlpI GC'TnA_GC
Cuts at: 2010 4396 2010
Size: 2386 4593
Bme1580I G_kGCm'C
Cuts at: 1790 2492 2865 2995 3023 3302 3702 5040 6196
Size: 702 373 130 28 279 400 1338 1156
Cuts at: 6196 1790
Size: 2573
Fragments arranged by size:
2573 1338 1156 702 400 373 279 130
28
BmgBI CAC'GTC
Cuts at: 1318 3853 4042 4164 1318
Size: 2535 189 122 4133
Fragments arranged by size:
4133 2535 189 122
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2859 4710 5668
Size: 416 53 83 399 24 1442 1851 958
Cuts at: 5668 442
Size: 1753
Fragments arranged by size:
1851 1753 1442 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 4241 1056
Size: 3185 3794
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2921 3018 3268 3381 3417 5639 1239
Size: 1682 97 250 113 36 2222 2579
Fragments arranged by size:
2579 2222 1682 250 113 97 36
Bpu10I CC'TnA_GC
Cuts at: 2559 3233 3899 2559
Size: 674 666 5639
Fragments arranged by size:
5639 674 666
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2552 3365 5104 5969 6267 2552
Size: 813 1739 865 298 3264
Fragments arranged by size:
3264 1739 865 813 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2580 5642 882
Size: 617 1081 3062 2219
Fragments arranged by size:
3062 2219 1081 617
BsaAI yAC'GTr
Cuts at: 493 1204 2368 2917 4770 493
Size: 711 1164 549 1853 2702
Fragments arranged by size:
2702 1853 1164 711 549
BsaBI GATnn'nnATC
Cuts at: 6973 6973
Size: 6979
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2673 4786 5168 5803
Size: 53 83 186 936 1140 2113 382 635
Cuts at: 5803 275
Size: 1451
Fragments arranged by size:
2113 1451 1140 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5412 5810 6084 6231 6642 5412
Size: 398 274 147 411 5749
Fragments arranged by size:
5749 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2894 2924 4118 4148 1178
Size: 30 165 30 1491 30 1194 30 4009
Fragments arranged by size:
4009 1491 1194 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2666 2975 4180 1186
Size: 1480 309 1205 3985
Fragments arranged by size:
3985 1480 1205 309
BseYI C'CCAG_C
Cuts at: 2048 2339 2719 2782 2842 3503 4224 6182 6678
Size: 291 380 63 60 661 721 1958 496
Cuts at: 6678 2048
Size: 2349
Fragments arranged by size:
2349 1958 721 661 496 380 291 63
60
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2576 2957 3800 1163
Size: 573 840 381 843 4342
Fragments arranged by size:
4342 843 840 573 381
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2548 4429 5190 6218 1540
Size: 275 186 547 1881 761 1028 2301
Fragments arranged by size:
2301 1881 1028 761 547 275 186
BsiHKAI G_wGCw'C
Cuts at: 2210 2257 5040 5125 6196 2210
Size: 47 2783 85 1071 2993
Fragments arranged by size:
2993 2783 1071 85 47
BsiWI C'GTAC_G
Cuts at: 2055 2055
Size: 6979
BsmI GAATG_Cn'
Cuts at: 4490 4583 4490
Size: 93 6886
BsmBI CGTCTCn'nnnn_
Cuts at: 3334 4170 3334
Size: 836 6143
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2016 2186 2210 2257 2473 2492 2856
Size: 399 226 170 24 47 216 19 364
Cuts at: 2856 2865 2995 3023 3066 3302 3624 3702 4298
Size: 9 130 28 43 236 322 78 596
Cuts at: 4298 5040 5125 6196 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 596 399 364 322 236
226 216 170 130 85 78 47 43
28 24 19 9
BspEI T'CCGG_A
Cuts at: 6642 6642
Size: 6979
BspHI T'CATG_A
Cuts at: 4868 6598 4868
Size: 1730 5249
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2598 4334 844
Size: 1754 1736 3489
Fragments arranged by size:
3489 1754 1736
BsrBI CCG'CTC
Cuts at: 1394 1823 3488 3582 4866 1394
Size: 429 1665 94 1284 3507
Fragments arranged by size:
3507 1665 1284 429 94
BsrDI GCAATG_nn'
Cuts at: 65 1248 4189 5474 5648 65
Size: 1183 2941 1285 174 1396
Fragments arranged by size:
2941 1396 1285 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4196 5623 5810 1700
Size: 110 2386 1427 187 2869
Fragments arranged by size:
2869 2386 1427 187 110
BsrGI T'GTAC_A
Cuts at: 96 4134 96
Size: 4038 2941
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6979
BssSI C'ACGA_G
Cuts at: 5039 6051 5039
Size: 1012 5967
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6979
BstBI TT'CG_AA
Cuts at: 2028 5783 2028
Size: 3755 3224
BstEII G'GTnAC_C
Cuts at: 1184 3907 1184
Size: 2723 4256
BstXI CCAn_nnnn'nTGG
Cuts at: 3631 4046 3631
Size: 415 6564
BstYI r'GATC_y
Cuts at: 1972 2168 2292 2838 3802 4309 4676 5061 5078
Size: 196 124 546 964 507 367 385 17
Cuts at: 5078 6530 6616 6628 6974 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 964 546 507 385 367 346
196 124 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2484 2529 2484
Size: 45 6934
BtgI C'CryG_G
Cuts at: 513 1065 2004 3092 3416 4117 4169 513
Size: 552 939 1088 324 701 52 3323
Fragments arranged by size:
3323 1088 939 701 552 324 52
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3183 487
Size: 683 56 1957 4283
Fragments arranged by size:
4283 1957 683 56
BtsI GCAGTG_nn'
Cuts at: 3642 3874 4495 5289 5309 3642
Size: 232 621 794 20 5312
Fragments arranged by size:
5312 794 621 232 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6979
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6944
DraI TTT'AAA
Cuts at: 4283 4283
Size: 6979
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2672 5986 809
Size: 507 1356 3314 1802
Fragments arranged by size:
3314 1802 1356 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2545 3958 4263
Size: 54 1425 50 275 18 715 1413 305
Cuts at: 4263 4426 5315 6944 8
Size: 163 889 1629 43
Fragments arranged by size:
1629 1425 1413 889 715 305 275 163
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2545 4426 1537
Size: 275 733 1881 4090
Fragments arranged by size:
4090 1881 733 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3337 4909 1353
Size: 1984 1572 3423
Fragments arranged by size:
3423 1984 1572
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3658 4065 4139 5566 5940 6086
Size: 1064 138 2069 407 74 1427 374 146
Cuts at: 6086 387
Size: 1280
Fragments arranged by size:
2069 1427 1280 1064 407 374 146 138
74
EcoICRI GAG'CTC
Cuts at: 2255 2255
Size: 6979
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2334 2633 2921 3018 3268 3381 3417
Size: 393 702 299 288 97 250 113 36
Cuts at: 3417 3734 3773 5042 5639 6426 6694 1239
Size: 317 39 1269 597 787 268 1524
Fragments arranged by size:
1524 1269 787 702 597 393 317 299
288 268 250 113 97 39 36
EcoNI CCTnn'n_nnAGG
Cuts at: 2483 2594 3902 4050 2483
Size: 111 1308 148 5412
Fragments arranged by size:
5412 1308 148 111
EcoO109I rG'GnC_Cy
Cuts at: 2426 2488 2489 2525 2991 2992 3019 3020 3115
Size: 62 1 36 466 1 27 1 95
Cuts at: 3115 4020 2426
Size: 905 5385
Fragments arranged by size:
5385 905 466 95 62 36 27 1
1 1
EcoRI G'AATT_C
Cuts at: 4288 4666 4288
Size: 378 6601
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4006 4102 4006
Size: 96 6883
FspI TGC'GCA
Cuts at: 5485 5485
Size: 6979
HaeII r_GCGC'y
Cuts at: 1678 6126 1678
Size: 4448 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2026 2058 2153 2185 2669
Size: 32 135 32 454 32 95 32 484
Cuts at: 2669 2701 2804 2836 4235 4267 5620 5652 5694
Size: 32 103 32 1399 32 1353 32 42
Cuts at: 5694 5726 1373
Size: 32 2626
Fragments arranged by size:
2626 1399 1353 484 454 135 103 95
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3895 4323 4569 669
Size: 988 281 1957 428 246 3079
Fragments arranged by size:
3079 1957 988 428 281 246
HindIII A'AGCT_T
Cuts at: 748 3983 748
Size: 3235 3744
HpaI GTT'AAC
Cuts at: 4569 4569
Size: 6979
KpnI G_GTAC'C
Cuts at: 4304 4304
Size: 6979
MfeI C'AATT_G
Cuts at: 4578 4578
Size: 6979
MluI A'CGCG_T
Cuts at: 6855 6855
Size: 6979
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2237 3301 3626 6093 6277 1305
Size: 932 1064 325 2467 184 2007
Fragments arranged by size:
2467 2007 1064 932 325 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3960 4265 10
Size: 54 1425 343 2128 305 2724
Fragments arranged by size:
2724 2128 1425 343 305 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4208 4937 5296 5455 518
Size: 648 3042 729 359 159 2042
Fragments arranged by size:
3042 2042 729 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2219 2312 2611 2786 3094 3418 3540 4395
Size: 613 93 299 175 308 324 122 855
Cuts at: 4395 5072 6220 6465 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 855 677 613 324 308 299
245 175 122 93
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5488
Fragments arranged by size:
5488 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6979
NheI G'CTAG_C
Cuts at: 1052 4237 1052
Size: 3185 3794
NotI GC'GGCC_GC
Cuts at: 2545 4426 2545
Size: 1881 5098
NspI r_CATG'y
Cuts at: 1132 1229 2464 4337 5882 1132
Size: 97 1235 1873 1545 2229
Fragments arranged by size:
2229 1873 1545 1235 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6979
PciI A'CATG_T
Cuts at: 5878 5878
Size: 6979
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3956 1108
Size: 2848 4131
PfoI T'CCnGG_A
Cuts at: 2400 2400
Size: 6979
PmeI GTTT'AAAC
Cuts at: 4283 4283
Size: 6979
PmlI CAC'GTG
Cuts at: 2368 2917 2368
Size: 549 6430
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5048 5080 6561 6593 5048
Size: 32 1481 32 5434
Fragments arranged by size:
5434 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2525 3115 4020 2525
Size: 590 905 5484
Fragments arranged by size:
5484 905 590
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6979
PsiI TTA'TAA
Cuts at: 4549 4549
Size: 6979
PspOMI G'GGCC_C
Cuts at: 2488 2861 2991 3019 2488
Size: 373 130 28 6448
Fragments arranged by size:
6448 373 130 28
PstI C_TGCA'G
Cuts at: 830 2151 3687 4331 830
Size: 1321 1536 644 3478
Fragments arranged by size:
3478 1536 1321 644
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6979
PvuII CAG'CTG
Cuts at: 2312 2312
Size: 6979
SacI G_AGCT'C
Cuts at: 2257 2257
Size: 6979
SacII CC_GC'GG
Cuts at: 3095 3419 3095
Size: 324 6655
SalI G'TCGA_C
Cuts at: 1936 4321 1936
Size: 2385 4594
SanDI GG'GwC_CC
Cuts at: 3115 4020 3115
Size: 905 6074
SbfI CC_TGCA'GG
Cuts at: 4331 4331
Size: 6979
ScaI AGT'ACT
Cuts at: 5227 6939 5227
Size: 1712 5267
SfcI C'TryA_G
Cuts at: 826 1045 2147 3544 3683 4327 5462 6143 6334
Size: 219 1102 1397 139 644 1135 681 191
Cuts at: 6334 826
Size: 1471
Fragments arranged by size:
1471 1397 1135 1102 681 644 219 191
139
SfiI GGCCn_nnn'nGGCC
Cuts at: 3444 3444
Size: 6979
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6979
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6979
SmaI CCC'GGG
Cuts at: 1872 3017 3617 3858 4306 1872
Size: 1145 600 241 448 4545
Fragments arranged by size:
4545 1145 600 448 241
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2567 3380 5083 5984 6246 820
Size: 197 926 624 813 1703 901 262 1553
Fragments arranged by size:
1703 1553 926 901 813 624 262 197
SnaBI TAC'GTA
Cuts at: 493 4770 493
Size: 4277 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6979
SphI G_CATG'C
Cuts at: 1132 2464 4337 1132
Size: 1332 1873 3774
Fragments arranged by size:
3774 1873 1332
SspI AAT'ATT
Cuts at: 5 52 4903 5
Size: 47 4851 2081
Fragments arranged by size:
4851 2081 47
StuI AGG'CCT
Cuts at: 1517 2714 3226 1517
Size: 1197 512 5270
Fragments arranged by size:
5270 1197 512
StyI C'CwwG_G
Cuts at: 513 1065 2004 2709 4418 513
Size: 552 939 705 1709 3074
Fragments arranged by size:
3074 1709 939 705 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2944 3517 3625 3667 4052 5006 5023
Size: 1255 1364 573 108 42 385 954 17
Cuts at: 5023 5176 5361 5806 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1364 1255 954 573 445 385 185
153 108 42 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4134 5225 6937
Size: 274 80 33 51 1443 2157 1091 1712
Cuts at: 6937 96
Size: 138
Fragments arranged by size:
2157 1712 1443 1091 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3451 3483 6939 6971 3451
Size: 32 3456 32 3459
Fragments arranged by size:
3459 3456 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4001 1316
Size: 2685 4294
XbaI T'CTAG_A
Cuts at: 4315 4315
Size: 6979
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3824 4013 4273 3824
Size: 189 260 6530
Fragments arranged by size:
6530 260 189
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6979
XmaI C'CCGG_G
Cuts at: 1870 3015 3615 3856 4304 1870
Size: 1145 600 241 448 4545
Fragments arranged by size:
4545 1145 600 448 241
XmnI GAAnn'nnTTC
Cuts at: 5108 5108
Size: 6979
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2674 4787 276
Size: 53 83 186 936 1140 2113 2468
Fragments arranged by size:
2468 2113 1140 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SacII SalI SanDI SbfI ScaI
SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BstZ17I DraIII EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PspXI PsrI
RsrII SapI SexAI SfoI SrfI SwaI